FastQCFastQC Report
Fri 27 May 2016
SRR2139936_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139936_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8022745
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115350.048.899091
GTATCAA203150.034.530891
ATCAACG252650.027.1498643
TCAACGC256050.026.8052774
CAACGCA259300.026.4221255
TATCAAC261800.026.4213562
AACGCAG268750.025.7508546
TACATGG337900.023.8867192
GTACATG347400.023.6090431
ACATGGG343000.023.18473
ACGCAGA307500.022.2936337
CGCAGAG311700.021.9801548
CATGGGG214300.020.381134
GCAGAGT366850.018.753499
GTGGTAT56250.018.1989941
GAGTACT195850.017.94912712-13
CAGAGTA357650.017.2918910-11
AGAGTAC338650.017.06987210-11
ATGGGGG137350.016.3600225
ACCGAAC9450.015.9671778
Produced by FastQC (version 0.11.5)