##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139936_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8022745
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37779463263509	33.0	31.0	34.0	31.0	34.0
2	32.533325065174076	34.0	31.0	34.0	31.0	34.0
3	32.65012124902387	34.0	31.0	34.0	31.0	34.0
4	36.16895439154554	37.0	35.0	37.0	35.0	37.0
5	36.0116456399898	37.0	35.0	37.0	35.0	37.0
6	36.06049512978413	37.0	35.0	37.0	35.0	37.0
7	36.03843123519444	37.0	35.0	37.0	35.0	37.0
8	36.03027018807154	37.0	35.0	37.0	35.0	37.0
9	37.873864618656086	39.0	38.0	39.0	35.0	39.0
10-11	37.75171577807845	39.0	37.5	39.0	35.0	39.0
12-13	37.76644109964856	39.0	37.5	39.0	35.0	39.0
14-15	39.07826336247756	40.0	38.5	41.0	36.0	41.0
16-17	39.03272732711809	40.0	38.0	41.0	36.0	41.0
18-19	39.11208457952982	40.0	38.5	41.0	36.0	41.0
20-21	39.11745121346438	40.0	39.0	41.0	36.0	41.0
22-23	39.07813098169791	40.0	39.0	41.0	36.0	41.0
24-25	39.058102101085666	40.0	39.0	41.0	36.0	41.0
26-27	38.960371753693295	40.0	38.0	41.0	35.0	41.0
28-29	38.88110928983766	40.0	38.0	41.0	35.0	41.0
30-31	38.73110088891515	40.0	38.0	41.0	35.0	41.0
32-33	38.63485153406623	40.0	38.0	41.0	35.0	41.0
34-35	38.592608673253864	40.0	38.0	41.0	35.0	41.0
36-37	38.49756134317663	40.0	38.0	41.0	34.5	41.0
38-39	38.46563178838616	40.0	38.0	41.0	34.0	41.0
40-41	38.46858839078634	40.0	38.0	41.0	34.0	41.0
42-43	38.43584941787209	40.0	38.0	41.0	34.0	41.0
44-45	38.331792060449985	40.0	38.0	41.0	34.0	41.0
46-47	38.28420282133303	40.0	38.0	41.0	34.0	41.0
48-49	38.2624792578152	40.0	38.0	41.0	34.0	41.0
50-51	38.19486556855241	40.0	37.5	41.0	33.5	41.0
52-53	38.06990547095582	40.0	37.0	41.0	33.0	41.0
54-55	37.90278343947179	40.0	37.0	41.0	33.0	41.0
56-57	37.78364574815522	39.5	37.0	41.0	33.0	41.0
58-59	37.56631123732318	39.0	36.0	41.0	33.0	41.0
60-61	37.41125566028977	39.0	36.0	40.5	32.5	41.0
62-63	37.2042290932838	39.0	35.0	40.0	32.0	41.0
64-65	36.890036731883555	38.5	35.0	40.0	31.5	41.0
66-67	36.82702250251798	38.0	35.0	40.0	32.0	41.0
68-69	36.59712382466158	37.5	35.0	40.0	32.0	41.0
70-71	36.29442655256143	37.0	35.0	39.5	31.0	41.0
72-73	35.988778121827124	36.5	35.0	39.0	31.0	41.0
74-75	35.593173400125224	36.0	34.0	39.0	31.0	40.0
76-77	33.5419709091155	34.5	32.0	36.5	28.0	39.0
78-79	34.73901732620787	35.0	34.0	37.0	30.0	39.0
80-81	34.746209954037326	35.0	34.0	37.0	31.0	39.0
82-83	34.456031746321216	35.0	34.0	36.5	30.0	39.0
84-85	34.19575870291469	35.0	34.0	36.0	30.0	37.0
86-87	33.98576635800232	35.0	34.0	36.0	30.0	37.0
88-89	33.73989973433013	35.0	34.0	35.0	30.0	37.0
90-91	33.565804119092675	35.0	33.5	35.0	30.0	36.0
92-93	33.501135957544534	35.0	33.0	35.0	30.0	36.0
94-95	33.47232515207075	35.0	33.0	35.0	30.0	36.0
96-97	33.49962776021849	35.0	33.0	35.0	30.0	36.0
98-99	33.52378102163203	35.0	33.0	35.0	30.0	35.0
100-101	32.267342060213494	34.0	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	6.0
20	37.0
21	179.0
22	595.0
23	2184.0
24	5510.0
25	12096.0
26	22373.0
27	36916.0
28	55907.0
29	78449.0
30	106103.0
31	139865.0
32	185346.0
33	255033.0
34	373923.0
35	568180.0
36	1013018.0
37	1866568.0
38	2262610.0
39	1021393.0
40	16449.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.53941761828401	20.410520339360154	13.412828152957623	23.637233889398203
2	14.749950949754847	21.963303685462154	41.08351305958103	22.20323230520197
3	18.000845097809474	27.38681547700661	30.653953636787435	23.958385788396484
4	11.98065150776343	18.29761737380627	39.80702861764005	29.914702500790252
5	12.366161551950134	38.95710339071528	35.94269383546652	12.734041221868061
6	28.713401709763925	38.83074932582302	19.51024244195721	12.945606522455844
7	25.872485258470824	33.217392106415474	23.9686351663016	16.9414874688121
8	24.570238520519137	36.43978727377022	21.865041545832543	17.1249326598781
9	26.181379428973145	16.06194392439491	21.468625432208945	36.288051214423
10-11	23.918722037687743	27.026550341494904	29.45746531375407	19.59726230706328
12-13	24.888039799982973	24.793539395693504	29.483079111478066	20.835341692845454
14-15	21.944430733424873	26.007735009368364	27.371146330981023	24.67668792622574
16-17	21.19670248684594	29.055409875400624	27.93018570714082	21.81770193061262
18-19	21.10424438321851	28.30663049948995	29.81437523121765	20.77474988607389
20-21	22.319010736976818	27.469703174847627	29.498244076457514	20.71304201171804
22-23	21.835616421279124	27.457865667539096	29.366721248020205	21.339796663161575
24-25	21.465689812796278	27.564118271168915	29.560173222035985	21.410018693998822
26-27	21.423920627952008	27.80360892503877	29.40184644359185	21.370624003417372
28-29	21.31753920124513	27.98642110726004	29.395707930057373	21.30033176143745
30-31	21.676523389544787	27.81129949200849	29.30718852344861	21.204988594998117
32-33	21.388607940734406	27.974711464030406	29.209729483813778	21.42695111142141
34-35	21.443365544763758	28.095012262962825	29.25175430685723	21.209867885416188
36-37	21.56662090283377	27.972057575099058	29.17578262247072	21.28553889959645
38-39	21.329007320806294	28.108914706930715	29.214076120087977	21.34800185217501
40-41	21.54663083345117	28.008917902156366	29.101816730558355	21.342634533834108
42-43	21.570219097005616	28.075333769114774	28.87353770553414	21.48090942834547
44-45	21.633271077881684	28.392828739515735	28.485896982068137	21.488003200534447
46-47	21.64553229731232	28.23074053549075	28.306384477932987	21.817342689263945
48-49	21.700150860777388	28.31496115447606	28.299883267531676	21.685004717214877
50-51	21.59891868124935	28.389528767995863	28.106103883580175	21.90544866717461
52-53	21.741404806393906	28.3540321803001	28.021534578567113	21.88302843473888
54-55	21.808155749307563	28.32328058281977	28.01589779040272	21.852665877469946
56-57	21.831826641316187	28.4219269770703	27.93143857456963	21.814807807043888
58-59	21.768531719173854	28.499583736730106	27.993817679161648	21.738066864934392
60-61	21.83016156606719	28.44435219487829	27.91438433814796	21.81110190090656
62-63	21.795153622786298	28.36664367752	27.932583075418627	21.905619624275076
64-65	21.727697543087178	28.391483013089765	27.968251423243778	21.912568020579286
66-67	21.856212545920734	28.441233660673504	27.757395840509545	21.945157952896217
68-69	21.880063909051785	28.391744120982043	27.846470410155717	21.88172155981045
70-71	21.80022387464284	28.14207745241084	27.93094486316709	22.126753809779228
72-73	21.903834968670903	28.206719451485913	28.03202230582918	21.857423274014003
74-75	21.754783973044137	28.31262031254521	27.887387295125272	22.04520841928538
76-77	21.767956256582433	28.257662414750417	27.837103997790567	22.13727733087658
78-79	21.84295830207773	28.248597921156325	27.823630481776718	22.084813294989225
80-81	21.789533672319966	28.227617319026027	27.854280555211197	22.128568453442814
82-83	21.889235578311858	28.1017427460116	27.989537273506297	22.019484402170246
84-85	22.118908818576255	28.072930064282264	27.884215678841983	21.9239454382995
86-87	21.789195703141058	28.136187512113548	27.95779243950879	22.1168243452366
88-89	22.0011392772807	28.05755213486959	27.857482164648538	22.08382642320117
90-91	22.06245570178731	27.984570060372217	27.96798140154364	21.984992836296833
92-93	22.064435694256552	27.913405064175418	27.966408843265057	22.05575039830298
94-95	21.869792226159777	28.006767437180237	28.0471323219517	22.07630801470829
96-97	21.853566023583156	28.05722038980363	28.10557275435756	21.983640832255656
98-99	22.06306745771283	28.645221926246776	28.054460616678885	21.23724999936151
100-101	26.017531154274533	35.56259210145364	28.861467471882936	9.558409272388896
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1346.0
1	1414.0
2	2643.0
3	4086.0
4	5866.0
5	10061.5
6	13422.5
7	15329.0
8	16219.0
9	15331.0
10	13465.0
11	11689.0
12	12553.0
13	17091.0
14	27540.0
15	41607.0
16	52587.5
17	58273.5
18	58794.0
19	55032.0
20	49585.0
21	44693.5
22	42607.0
23	45712.5
24	54645.5
25	68841.0
26	87920.0
27	108164.5
28	127092.5
29	146728.5
30	163342.0
31	178395.0
32	195842.0
33	215206.0
34	235987.5
35	255537.5
36	275416.0
37	290579.0
38	299666.5
39	308977.0
40	319397.0
41	324812.0
42	326889.5
43	332776.5
44	339165.0
45	344413.0
46	346104.0
47	346585.5
48	345022.0
49	338857.5
50	331293.0
51	319060.5
52	301925.5
53	286819.5
54	271718.5
55	249647.5
56	226046.0
57	201704.5
58	175630.5
59	151378.5
60	128281.5
61	104577.0
62	81223.0
63	62217.5
64	47072.5
65	35391.5
66	26912.0
67	20569.0
68	15778.5
69	11213.5
70	7580.0
71	5417.5
72	3794.0
73	2555.0
74	1779.0
75	1268.0
76	886.0
77	596.0
78	414.5
79	288.0
80	199.5
81	148.0
82	113.5
83	85.0
84	64.5
85	43.5
86	32.0
87	29.5
88	23.0
89	15.0
90	8.0
91	4.0
92	3.5
93	3.5
94	3.5
95	2.5
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004474777647800098
3	6.232280846518243E-5
4	1.2464561693036487E-5
5	1.620393020094743E-4
6	0.0
7	2.4929123386072973E-5
8	4.8611790602842296E-4
9	6.232280846518243E-5
10-11	5.6090527618664186E-5
12-13	3.490077274050216E-4
14-15	1.2464561693036487E-5
16-17	4.0509825502368577E-4
18-19	1.2464561693036487E-5
20-21	4.986026349528799E-5
22-23	0.0
24-25	0.0
26-27	5.614107578652243E-5
28-29	0.0
30-31	5.0052097977482316E-5
32-33	3.5159816749546515E-4
34-35	0.0
36-37	2.8560002756992264E-4
38-39	0.0010570200835737734
40-41	0.0
42-43	0.0
44-45	2.850981705714508E-4
46-47	0.0
48-49	0.0
50-51	8.992995494163373E-5
52-53	5.252035373788758E-4
54-55	0.003224240620471448
56-57	0.0035744734362791256
58-59	0.004569155551662234
60-61	9.957543248376311E-4
62-63	7.474524205424441E-6
64-65	5.299072457927068E-4
66-67	0.0
68-69	0.0
70-71	7.899598629293244E-6
72-73	1.763480970357007E-4
74-75	1.6255703516775156E-5
76-77	0.0
78-79	0.0
80-81	5.692890157978552E-4
82-83	0.0
84-85	0.0
86-87	8.898840561164445E-6
88-89	0.0
90-91	0.0012434431401084098
92-93	1.5023192052731406E-4
94-95	2.8722632716514795E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	217.0
20-21	618.0
22-23	1355.0
24-25	3908.0
26-27	5792.0
28-29	12973.0
30-31	26796.0
32-33	32914.0
34-35	44260.0
36-37	67974.0
38-39	83387.0
40-41	81532.0
42-43	93347.0
44-45	108746.0
46-47	107989.0
48-49	100029.0
50-51	90113.0
52-53	83267.0
54-55	88216.0
56-57	93608.0
58-59	93367.0
60-61	91260.0
62-63	85030.0
64-65	85815.0
66-67	92026.0
68-69	94964.0
70-71	93685.0
72-73	86623.0
74-75	87076.0
76-77	86325.0
78-79	91380.0
80-81	94076.0
82-83	88032.0
84-85	85532.0
86-87	88989.0
88-89	95691.0
90-91	105778.0
92-93	100954.0
94-95	107919.0
96-97	155350.0
98-99	615939.0
100-101	4369893.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.82855562020896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.78553187831976	74.42762897331563
2	7.5578290628363884	12.671237879240241
3	2.0085419065432477	5.051195007845435
4	0.7997024526050241	2.6815160611127116
5	0.3662545040323562	1.5351293031214213
6	0.182909337654734	0.9199815351027272
7	0.09987238008255328	0.5860510158071077
8	0.06210571889592851	0.41649861686403244
9	0.03654759873373467	0.2757359171912359
>10	0.09890853796122319	1.176501509492318
>50	0.0011814521508742904	0.06728674153117437
>100	4.7421368331502367E-4	0.07065336508601505
>500	9.397104429660954E-5	0.05689492394629438
>1k	4.6985456636291434E-5	0.0636891503436579
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2464561693036485E-5
2	0.0	1.2464561693036485E-5	0.0	0.0	1.2464561693036485E-5
3	0.0	1.2464561693036485E-5	0.0	0.0	1.2464561693036485E-5
4	0.0	4.985824677214594E-5	0.0	0.0	1.2464561693036485E-5
5	0.0	4.985824677214594E-5	0.0	0.0	1.2464561693036485E-5
6	0.0	4.985824677214594E-5	0.0	0.0	1.2464561693036485E-5
7	0.0	4.985824677214594E-5	0.0	1.2464561693036485E-5	2.492912338607297E-5
8	0.0	4.985824677214594E-5	0.0	1.2464561693036485E-5	3.7393685079109455E-5
9	0.0	6.232280846518243E-5	0.0	1.2464561693036485E-5	3.7393685079109455E-5
10-11	0.0	6.232280846518243E-5	0.0	1.8696842539554728E-5	3.7393685079109455E-5
12-13	0.0	6.232280846518243E-5	0.0	2.492912338607297E-5	3.7393685079109455E-5
14-15	0.0	8.101965100473716E-5	0.0	2.492912338607297E-5	7.478737015821891E-5
16-17	0.0	8.72519318512554E-5	0.0	3.1161404232591216E-5	7.478737015821891E-5
18-19	0.0	9.971649354429188E-5	0.0	6.232280846518243E-5	8.101965100473716E-5
20-21	0.0	1.1218105523732837E-4	0.0	6.232280846518243E-5	8.72519318512554E-5
22-23	0.0	1.1841333608384662E-4	0.0	8.101965100473716E-5	8.72519318512554E-5
24-25	0.0	1.2464561693036486E-4	0.0	2.181298296281385E-4	8.72519318512554E-5
26-27	0.0	1.2464561693036486E-4	0.0	4.237950975632405E-4	8.72519318512554E-5
28-29	0.0	1.2464561693036486E-4	0.0	0.0011592042374523933	8.72519318512554E-5
30-31	0.0	1.3711017862340136E-4	0.0	0.0027609004150075814	8.72519318512554E-5
32-33	0.0	1.3711017862340136E-4	0.0	0.005228883630228806	8.72519318512554E-5
34-35	0.0	1.745038637025108E-4	0.0	0.008613012129888211	9.971649354429188E-5
36-37	0.0	2.1189754878162025E-4	0.0	0.013829431198423982	1.1218105523732837E-4
38-39	0.0	2.1189754878162025E-4	0.0	0.022218081217837537	1.1218105523732837E-4
40-41	0.0	2.3682667216769324E-4	0.0	0.03147301827491713	1.1218105523732837E-4
42-43	0.0	2.6798807640028446E-4	0.0	0.03931322757983707	1.1841333608384662E-4
44-45	0.0	3.178463231724304E-4	0.0	0.04782029093533448	1.2464561693036486E-4
46-47	0.0	3.365431657119851E-4	0.0	0.05822819994901994	1.2464561693036486E-4
48-49	0.0	3.365431657119851E-4	0.0	0.0679505580695884	1.2464561693036486E-4
50-51	0.0	3.614722890980581E-4	0.0	0.07752957373068695	1.2464561693036486E-4
52-53	0.0	3.614722890980581E-4	0.0	0.08636694797104981	1.2464561693036486E-4
54-55	0.0	3.614722890980581E-4	0.0	0.09509837343702186	1.2464561693036486E-4
56-57	0.0	3.614722890980581E-4	0.0	0.10343716520966328	1.308778977768831E-4
58-59	0.0	3.614722890980581E-4	0.0	0.1121561261139423	1.3711017862340136E-4
60-61	0.0	3.614722890980581E-4	0.0	0.12068188631197926	1.3711017862340136E-4
62-63	0.0	3.614722890980581E-4	0.0	0.12905807176969977	1.3711017862340136E-4
64-65	0.0	3.988659741771675E-4	0.0	0.13711641090424787	1.3711017862340136E-4
66-67	0.0	4.0509825502368577E-4	0.0	0.14502517529847953	1.3711017862340136E-4
68-69	0.0	4.1756281671672226E-4	0.0	0.15285915232255293	1.433424594699196E-4
70-71	0.0	4.3002737840975875E-4	0.0	0.1599577202067372	1.4957474031643782E-4
72-73	0.0	4.36259659256277E-4	0.0	0.16595317438108775	1.4957474031643782E-4
74-75	0.0	4.487242209493135E-4	0.0	0.17079565659883245	1.4957474031643782E-4
76-77	0.0	4.798856251819047E-4	0.0	0.17508969810208352	1.4957474031643782E-4
78-79	0.0	4.8611790602842296E-4	0.0	0.17910951924808777	1.4957474031643782E-4
80-81	0.0	4.8611790602842296E-4	0.0	0.18077977051495467	1.5580702116295607E-4
82-83	0.0	4.923501868749412E-4	0.0	0.18155880562076943	1.620393020094743E-4
84-85	0.0	4.985824677214595E-4	0.0	0.18223189195219341	1.6827158285599256E-4
86-87	0.0	5.11047029414496E-4	0.0	0.18254973827536586	1.745038637025108E-4
88-89	0.0	5.359761528005689E-4	0.0	0.1826432224880636	1.745038637025108E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11535	0.0	48.89909	1
GTATCAA	20315	0.0	34.53089	1
ATCAACG	25265	0.0	27.149864	3
TCAACGC	25605	0.0	26.805277	4
CAACGCA	25930	0.0	26.422125	5
TATCAAC	26180	0.0	26.421356	2
AACGCAG	26875	0.0	25.750854	6
TACATGG	33790	0.0	23.886719	2
GTACATG	34740	0.0	23.609043	1
ACATGGG	34300	0.0	23.1847	3
ACGCAGA	30750	0.0	22.293633	7
CGCAGAG	31170	0.0	21.980154	8
CATGGGG	21430	0.0	20.38113	4
GCAGAGT	36685	0.0	18.75349	9
GTGGTAT	5625	0.0	18.198994	1
GAGTACT	19585	0.0	17.949127	12-13
CAGAGTA	35765	0.0	17.29189	10-11
AGAGTAC	33865	0.0	17.069872	10-11
ATGGGGG	13735	0.0	16.360022	5
ACCGAAC	945	0.0	15.967177	8
>>END_MODULE