FastQCFastQC Report
Fri 27 May 2016
SRR2139935_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139935_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24817234
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA343250.041.1615371
GTATCAA558000.031.2869281
ATCAACG709450.023.9844023
TCAACGC724150.023.5311284
CAACGCA722900.023.4820615
TATCAAC739850.023.3117922
AACGCAG743900.022.9936146
CTTATAC443650.021.5996741
TATACAC507950.020.821013
TACATGG784200.020.0593532
ACGCAGA851450.019.951087
CGCAGAG865650.019.6238068
GTACATG820000.019.5297761
ACATGGG795000.019.4038813
CGCTACG8000.019.17717694-95
TTATACA579300.017.4939022
CATGGGG556100.017.377464
GCAGAGT994600.016.8186349
TATGCCG306450.016.62020142-43
ACACATC583750.016.5821486
Produced by FastQC (version 0.11.5)