FastQCFastQC Report
Fri 27 May 2016
SRR2139934_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139934_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14015043
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159250.046.4364131
GTATCAA275800.032.6768261
TCAACGC341600.025.6344284
ATCAACG343400.025.4760933
CAACGCA348150.025.1048725
TATCAAC363250.024.5268782
AACGCAG360750.024.3192836
ACGCAGA421450.020.767847
CGCAGAG426650.020.5629448
GTACATG384750.019.8085371
TACATGG378500.019.6789882
GTGGTAT80700.018.8676661
ACATGGG387650.018.714683
TGGTATC76850.017.3496692
CATGGGG291450.017.140544
GCAGAGT504450.017.1305399
AGAGTAC452000.017.05176210-11
GAGTACT294150.016.78735712-13
CAGAGTA492850.016.16025710-11
AGTACTT324600.015.23792512-13
Produced by FastQC (version 0.11.5)