##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139934_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14015043
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86243124619739	31.0	31.0	34.0	30.0	34.0
2	32.00080963005251	33.0	31.0	34.0	30.0	34.0
3	32.06600579106322	33.0	31.0	34.0	30.0	34.0
4	35.7632863488182	37.0	35.0	37.0	35.0	37.0
5	35.62031568508209	37.0	35.0	37.0	33.0	37.0
6	35.6190416968396	37.0	35.0	37.0	33.0	37.0
7	35.49524757077092	37.0	35.0	37.0	33.0	37.0
8	35.49226142224465	37.0	35.0	37.0	33.0	37.0
9	37.09326435887496	39.0	37.0	39.0	33.0	39.0
10-11	36.96874044553413	39.0	37.0	39.0	33.0	39.0
12-13	36.92759076800549	39.0	37.0	39.0	33.0	39.0
14-15	38.092710061610234	40.0	37.0	41.0	33.0	41.0
16-17	38.08910086825991	40.0	37.0	41.0	33.0	41.0
18-19	38.11465194933758	40.0	37.0	41.0	33.0	41.0
20-21	38.099029208598495	40.0	37.0	41.0	33.0	41.0
22-23	38.01127626024186	40.0	37.0	41.0	33.0	41.0
24-25	37.920534287286834	40.0	37.0	41.0	33.0	41.0
26-27	37.75090438123042	40.0	37.0	41.0	32.0	41.0
28-29	37.61434688934288	39.5	37.0	41.0	32.0	41.0
30-31	37.47696019481031	39.0	36.0	41.0	32.0	41.0
32-33	37.62872588625281	39.5	37.0	41.0	32.0	41.0
34-35	37.59410403923114	40.0	36.5	41.0	32.0	41.0
36-37	37.466961857159646	39.5	36.0	41.0	31.5	41.0
38-39	37.30695060715041	39.0	36.0	41.0	31.0	41.0
40-41	37.24847454225305	39.0	36.0	41.0	31.0	41.0
42-43	37.138852898766956	39.0	36.0	40.0	31.0	41.0
44-45	37.20372157018459	39.0	36.0	40.5	31.0	41.0
46-47	37.35559075265929	39.0	36.0	41.0	31.0	41.0
48-49	37.28453263000037	39.0	36.0	41.0	31.0	41.0
50-51	37.143308501719304	39.0	36.0	41.0	31.0	41.0
52-53	37.004286789888084	39.0	35.0	41.0	31.0	41.0
54-55	36.87156120620975	39.0	35.0	40.0	31.0	41.0
56-57	36.71643421647674	39.0	35.0	40.0	30.0	41.0
58-59	36.57590692498522	38.0	35.0	40.0	30.0	41.0
60-61	36.335607505409726	38.0	35.0	40.0	30.0	41.0
62-63	36.072467349776474	38.0	34.0	40.0	29.0	41.0
64-65	35.84643591544351	37.0	34.0	40.0	29.0	41.0
66-67	35.58634584876019	37.0	34.0	39.5	29.0	41.0
68-69	35.29778262019311	36.0	34.0	39.0	29.0	41.0
70-71	34.98809958144689	36.0	33.5	39.0	29.0	40.0
72-73	34.65815734146603	35.5	33.0	38.5	28.0	40.0
74-75	34.32354664525239	35.0	33.0	37.5	28.0	40.0
76-77	33.2021333639404	34.5	31.5	36.0	26.5	39.0
78-79	33.58858693358292	35.0	32.0	36.5	27.5	39.0
80-81	33.47713356873929	35.0	32.0	36.0	28.0	38.5
82-83	33.24946916657229	35.0	32.0	36.0	27.0	37.5
84-85	33.045235189943654	34.5	32.0	35.0	27.0	37.0
86-87	32.8421196110585	34.0	32.0	35.0	27.0	36.5
88-89	32.62228183053875	34.0	32.0	35.0	27.0	36.0
90-91	32.49608488964337	34.0	32.0	35.0	27.0	36.0
92-93	32.427534596372695	34.0	31.0	35.0	27.0	35.5
94-95	32.396594538363786	34.0	31.0	35.0	27.0	35.0
96-97	32.30268907427528	34.0	31.0	35.0	27.0	35.0
98-99	32.46152889268634	34.0	31.0	35.0	27.0	35.0
100-101	31.689106849019417	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	6.0
19	15.0
20	175.0
21	755.0
22	3491.0
23	11814.0
24	30514.0
25	62868.0
26	110992.0
27	171757.0
28	241268.0
29	316581.0
30	395772.0
31	485906.0
32	594855.0
33	740806.0
34	957573.0
35	1272367.0
36	1828012.0
37	2693945.0
38	2745634.0
39	1300707.0
40	49228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.38754543956804	21.838505953923935	15.077106791609559	24.696841814898463
2	14.21692563656819	22.164736781303848	41.00617336409276	22.612164218035197
3	17.49097737338373	26.642073092462148	31.286161590799257	24.580787943354864
4	12.51037902630766	18.721283980363097	38.28985041287422	30.478486580455016
5	12.905447382501785	38.111613357162014	35.938441287693514	13.044497972642683
6	30.188555254521876	38.35968965632143	18.293764778317126	13.157990310839573
7	25.988939170575502	32.810916099222815	23.572763922308337	17.62738080789335
8	23.928132079223733	37.86977321439542	21.010395758329103	17.191698948051748
9	25.245994607365812	16.541954241595977	21.45387638125691	36.7581747697813
10-11	24.20265496152955	26.877991027212687	29.121662345238615	19.797691666019148
12-13	24.680106225860314	24.502818150468748	29.2722683762012	21.544807247469734
14-15	21.829676155827705	25.95724465490402	26.709222369135794	25.50385682013248
16-17	21.566455415085063	28.9391548780835	27.537496674109384	21.956893032722054
18-19	21.593240396153483	28.199540885503392	29.10375991190881	21.10345880643431
20-21	22.292847940132848	27.455707005849934	28.90207220294738	21.34937285106984
22-23	22.069891951313437	27.39506351867452	28.755980700759288	21.77906382925276
24-25	21.861402865004134	27.526891023331352	28.832697822559595	21.77900828910492
26-27	21.865700543881488	27.62862186242613	28.73899866016773	21.766678933524652
28-29	21.789521279056867	27.73811040167126	28.752154237991512	21.720214081280357
30-31	22.08074726275417	27.56402249868434	28.619926059845437	21.735304178716053
32-33	21.89154176967676	27.761074451345337	28.56344890043368	21.783934878544226
34-35	21.99687069223956	27.725797163305643	28.594206364136177	21.68312578031862
36-37	21.937432003246084	27.716207294304994	28.56653871357373	21.77982198887519
38-39	21.823329802388866	27.76275340614384	28.63009825766154	21.78381853380575
40-41	22.030436583405802	27.606707442809707	28.489188960688523	21.87366701309597
42-43	21.957032080885106	27.76993875409651	28.258933630233646	22.014095534784733
44-45	22.027496709007746	27.84369938495952	28.09958433147243	22.02921957456031
46-47	22.126714569415107	27.762783000306428	27.89683942610614	22.213663004172325
48-49	22.04175432417774	27.946592603637672	27.841520832206562	22.17013223997802
50-51	22.036486732369358	28.02129374642515	27.764639295218064	22.177580225987427
52-53	22.187925515073818	27.880718279749715	27.66612079038802	22.265235414788435
54-55	22.1225807034845	27.814939537610133	27.698239020802056	22.364240738103312
56-57	22.15091285468726	27.85376927369285	27.69591981374108	22.299398057878804
58-59	22.08843857794932	27.92986971768969	27.71899481312946	22.262696891231528
60-61	22.180067270042944	27.872094146862608	27.702441262681482	22.245397320412966
62-63	22.12799767346018	28.02820971090175	27.614661273480507	22.22913134215757
64-65	22.09809184954606	28.001144834722382	27.59031901768097	22.31044429805059
66-67	22.14814729159508	28.025306838288422	27.433218954578148	22.39332691553835
68-69	22.104894461516146	27.985080329609552	27.48280785079464	22.427217358079666
70-71	22.11811304311295	27.793396522748242	27.540853603767108	22.5476368303717
72-73	22.13668003565937	27.792899489568633	27.500294812425256	22.570125662346737
74-75	22.065228817502568	27.848030492883595	27.513807207318308	22.57293348229553
76-77	22.27198744621544	27.800376690784063	27.39406525794012	22.533570605060376
78-79	22.249285997724705	27.773998183333948	27.355765980407458	22.62094983853389
80-81	22.202337275242208	27.818681728931793	27.357950860728813	22.621030135097183
82-83	22.261819583404765	27.76046269767212	27.460852462282737	22.516865256640372
84-85	22.3164572852143	27.78223856548547	27.412748542518194	22.488555606782032
86-87	22.193373918771282	27.783597727107214	27.481695478297834	22.541332875823667
88-89	22.213281866905838	27.783591709317708	27.504531523562083	22.49859490021438
90-91	22.275752304607753	27.74281834342085	27.55726864361628	22.42416070835512
92-93	22.293213661862428	27.63308195971848	27.625972763427036	22.447731614992055
94-95	22.10558824812936	27.65216666805162	27.725644475822076	22.516600607996942
96-97	22.01705898789827	27.677684653194	27.802627162732335	22.50262919617539
98-99	22.144411274327027	28.316137047948043	27.905581950366997	21.633869727357936
100-101	25.706358572139383	35.66634471794522	28.8459395782684	9.781357131646997
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3054.0
1	2812.0
2	3873.0
3	5511.0
4	7365.5
5	11483.5
6	15054.0
7	17767.0
8	19416.5
9	19304.5
10	18225.0
11	17124.5
12	18928.5
13	24787.5
14	38496.5
15	57555.0
16	72203.0
17	79007.5
18	80298.0
19	78627.5
20	75532.5
21	72777.0
22	73170.5
23	79759.5
24	93589.5
25	114699.0
26	141382.5
27	169374.0
28	200104.0
29	233067.5
30	262703.5
31	292598.0
32	324929.5
33	355273.0
34	384373.5
35	413430.0
36	446220.5
37	476276.5
38	498405.5
39	516074.0
40	532459.0
41	549740.0
42	562528.5
43	573975.5
44	585883.5
45	595207.0
46	601847.0
47	605518.5
48	602065.0
49	593114.0
50	582471.0
51	566009.0
52	542034.5
53	513391.0
54	483156.5
55	448634.5
56	411460.5
57	372790.5
58	331301.5
59	287612.0
60	241954.0
61	197902.0
62	157493.0
63	122669.0
64	93095.5
65	69425.5
66	52011.0
67	39356.5
68	29816.5
69	21519.5
70	15108.5
71	10793.5
72	7455.0
73	5066.0
74	3502.0
75	2374.5
76	1592.5
77	1055.5
78	735.5
79	518.5
80	366.0
81	272.0
82	211.0
83	148.0
84	96.0
85	71.0
86	57.5
87	48.0
88	33.0
89	21.5
90	12.5
91	7.5
92	8.0
93	5.5
94	2.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0034248913827806308
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	1.462714028062561E-4
20-21	0.001958703816154419
22-23	3.17570954717843E-4
24-25	1.0707546078853797E-5
26-27	2.356716628106974E-4
28-29	0.003479887696092476
30-31	3.5420393559928444E-4
32-33	6.099956762071187E-5
34-35	0.0020028782368907835
36-37	0.055381659407250684
38-39	0.033808925855846175
40-41	0.04267418513617717
42-43	8.282377522206798E-4
44-45	0.0024847683139341864
46-47	0.001398024060146034
48-49	5.420004197620273E-4
50-51	3.88623059920818E-6
52-53	9.539494449191945E-4
54-55	0.0022282549869099932
56-57	0.001254698454872926
58-59	0.003848466919202877
60-61	0.0014449164596098644
62-63	1.3298295096232903E-4
64-65	7.238279184973131E-4
66-67	4.224274318741893E-4
68-69	1.4720488872630927E-4
70-71	0.0011161702038276202
72-73	0.0016992681595458486
74-75	6.654904236607107E-4
76-77	0.0010943243191049714
78-79	1.4016744309306936E-4
80-81	0.0020959155596018504
82-83	0.002538882379140736
84-85	0.0038332271609781213
86-87	0.004044646887290877
88-89	0.07236191922299089
90-91	6.427958420829345E-4
92-93	4.81797709308851E-5
94-95	3.301877629223246E-5
96-97	0.008350127827777644
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	428.0
20-21	1448.0
22-23	2760.0
24-25	6044.0
26-27	7495.0
28-29	13157.0
30-31	37935.0
32-33	49878.0
34-35	70599.0
36-37	103787.0
38-39	109212.0
40-41	118111.0
42-43	134804.0
44-45	157439.0
46-47	157650.0
48-49	144557.0
50-51	133037.0
52-53	122279.0
54-55	136169.0
56-57	141595.0
58-59	148515.0
60-61	150747.0
62-63	147378.0
64-65	159612.0
66-67	169639.0
68-69	170024.0
70-71	169101.0
72-73	164951.0
74-75	170804.0
76-77	169349.0
78-79	180602.0
80-81	182221.0
82-83	177781.0
84-85	182315.0
86-87	194216.0
88-89	207411.0
90-91	223865.0
92-93	241850.0
94-95	315423.0
96-97	389856.0
98-99	1169341.0
100-101	7281658.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.8910943057758
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.01408415023006	76.41394106445219
2	6.861928952723995	11.65033315690452
3	1.6788915760872043	4.275688293443743
4	0.6397618211139752	2.1724032435768534
5	0.30490932491813716	1.2942043128168008
6	0.16426923424955325	0.8366997033729842
7	0.09687867200015161	0.5756895536688235
8	0.0641978339208711	0.4359859498882561
9	0.03868729914060402	0.29557864438026976
>10	0.13239940322612276	1.7448094797527334
>50	0.0032588736407600533	0.18814615536871282
>100	6.892486203713297E-4	0.0854519434818454
>500	2.9079393310870313E-5	0.01791906926939317
>1k	1.4530734860642767E-5	0.013149429622905941
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.281114228475788E-5	0.0	3.5675951903964906E-5	0.0
3	0.0	4.281114228475788E-5	0.0	4.281114228475788E-5	0.0
4	0.0	7.848709418872279E-5	0.0	7.848709418872279E-5	0.0
5	0.0	7.848709418872279E-5	0.0	7.848709418872279E-5	0.0
6	7.1351903807929805E-6	7.848709418872279E-5	0.0	7.848709418872279E-5	0.0
7	7.1351903807929805E-6	7.848709418872279E-5	0.0	9.275747495030876E-5	0.0
8	7.1351903807929805E-6	8.562228456951577E-5	0.0	9.275747495030876E-5	0.0
9	3.5675951903964906E-5	1.2843342685427364E-4	0.0	1.1416304609268769E-4	0.0
10-11	3.5675951903964906E-5	1.2843342685427364E-4	0.0	1.462714028062561E-4	0.0
12-13	4.994633266555087E-5	1.2843342685427364E-4	0.0	1.7481216432942804E-4	0.0
14-15	5.7081523046343844E-5	1.6767697394863505E-4	0.0	2.0335292585259995E-4	0.0
16-17	6.778430861753332E-5	1.81947354710221E-4	0.0	2.532992585181508E-4	0.0
18-19	7.135190380792981E-5	2.0335292585259995E-4	0.0	2.925428056125122E-4	0.0
20-21	8.562228456951578E-5	2.3546128256616837E-4	0.0	3.389215430876666E-4	0.0
22-23	9.989266533110173E-5	2.640020440893403E-4	0.0	5.101661122266981E-4	7.1351903807929805E-6
24-25	1.1416304609268769E-4	3.353539478972701E-4	0.0	0.0011844416032116348	7.1351903807929805E-6
26-27	1.2843342685427364E-4	3.8530028056282096E-4	0.0	0.003499810881778957	7.1351903807929805E-6
28-29	1.2843342685427364E-4	3.8530028056282096E-4	0.0	0.019083066673430828	7.1351903807929805E-6
30-31	1.462714028062561E-4	4.209762324667859E-4	0.0	0.02905449523058902	7.1351903807929805E-6
32-33	1.6410937875823857E-4	4.209762324667859E-4	0.0	0.03719574745507381	7.1351903807929805E-6
34-35	1.6767697394863505E-4	4.709225651323368E-4	0.0	0.048783296633481604	7.1351903807929805E-6
36-37	1.8551494990061751E-4	5.31571683369077E-4	0.0	0.06541185781591966	7.1351903807929805E-6
38-39	1.8551494990061751E-4	5.494096593210595E-4	0.0	0.09496938396835458	7.1351903807929805E-6
40-41	1.926501402814105E-4	6.171939679385928E-4	0.0	0.15039197525116405	7.1351903807929805E-6
42-43	2.0335292585259995E-4	6.421671342713682E-4	0.0	0.17667444901881502	7.1351903807929805E-6
44-45	2.1048811623339291E-4	7.063838476985051E-4	0.0	0.21582523863822609	7.1351903807929805E-6
46-47	2.1405571142378942E-4	7.49194989983263E-4	0.0	0.26078407322760266	7.1351903807929805E-6
48-49	2.1762330661418593E-4	7.527625851736594E-4	0.0	0.29851495996123595	7.1351903807929805E-6
50-51	2.2832609218537538E-4	8.740608216471402E-4	0.0	0.33492226887923215	7.1351903807929805E-6
52-53	2.3546128256616837E-4	8.847636072183297E-4	0.0	0.3814900888994775	7.1351903807929805E-6
54-55	2.3546128256616837E-4	8.883312024087261E-4	0.0	0.48044447669550494	7.1351903807929805E-6
56-57	2.4616406813735784E-4	8.990339879799156E-4	0.0	0.5800124908642806	7.1351903807929805E-6
58-59	2.497316633277543E-4	9.061691783607085E-4	0.0	0.6621135589808751	7.1351903807929805E-6
60-61	2.497316633277543E-4	9.31142344693484E-4	0.0	0.7075147753738608	7.1351903807929805E-6
62-63	2.6043444889894377E-4	9.703858917878453E-4	0.0	0.7324058870172571	7.1351903807929805E-6
64-65	2.7827242485092623E-4	0.001084548937880533	0.0	0.7511250589812675	7.1351903807929805E-6
66-67	2.8540761523171925E-4	0.001105954509022912	0.0	0.7618813584803129	7.1351903807929805E-6
68-69	2.925428056125122E-4	0.0011309276753556875	0.0	0.7722523576987955	7.1351903807929805E-6
70-71	2.996779959933052E-4	0.0011737388176404455	0.0	0.7820311361156722	7.1351903807929805E-6
72-73	2.996779959933052E-4	0.0011951443887828243	0.0	0.7904934719072927	7.1351903807929805E-6
74-75	2.996779959933052E-4	0.0012094147695444102	0.0	0.7984991555145424	7.1351903807929805E-6
76-77	2.996779959933052E-4	0.0012308203406867892	0.0	0.8060303489614695	7.1351903807929805E-6
78-79	3.0324559118370166E-4	0.0012415231262579787	0.0	0.8128515909655075	7.1351903807929805E-6
80-81	3.068131863740982E-4	0.0012415231262579787	0.0	0.8176000601639253	7.1351903807929805E-6
82-83	3.068131863740982E-4	0.0012415231262579787	0.0	0.8214209546128399	7.1351903807929805E-6
84-85	3.068131863740982E-4	0.001259361102209961	0.0	0.824895792328286	7.1351903807929805E-6
86-87	3.103807815644947E-4	0.0012736314829715472	0.0	0.8254559047731784	7.1351903807929805E-6
88-89	3.1394837675489116E-4	0.0012914694589235296	0.0	0.8255201214866055	7.1351903807929805E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15925	0.0	46.436413	1
GTATCAA	27580	0.0	32.676826	1
TCAACGC	34160	0.0	25.634428	4
ATCAACG	34340	0.0	25.476093	3
CAACGCA	34815	0.0	25.104872	5
TATCAAC	36325	0.0	24.526878	2
AACGCAG	36075	0.0	24.319283	6
ACGCAGA	42145	0.0	20.76784	7
CGCAGAG	42665	0.0	20.562944	8
GTACATG	38475	0.0	19.808537	1
TACATGG	37850	0.0	19.678988	2
GTGGTAT	8070	0.0	18.867666	1
ACATGGG	38765	0.0	18.71468	3
TGGTATC	7685	0.0	17.349669	2
CATGGGG	29145	0.0	17.14054	4
GCAGAGT	50445	0.0	17.130539	9
AGAGTAC	45200	0.0	17.051762	10-11
GAGTACT	29415	0.0	16.787357	12-13
CAGAGTA	49285	0.0	16.160257	10-11
AGTACTT	32460	0.0	15.237925	12-13
>>END_MODULE