FastQCFastQC Report
Fri 27 May 2016
SRR2139933_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139933_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10854103
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120650.046.7357751
GTATCAA206250.033.0022351
ACCGAGG110050.026.39722492-93
AGACCGA107200.026.24095590-91
TCAACGC253900.026.02994
GACCGAG110550.025.71456790-91
GAGACCG109050.025.70667688-89
ATCAACG258650.025.5677723
CGAGACC111250.025.25552288-89
CAACGCA262250.025.2324755
TATCAAC269300.024.8479462
AACGCAG270650.024.4797426
ACGAGAC118300.024.25707286-87
CACGAGA123250.022.98145586-87
CCCACGA124800.022.33969584-85
GCCCACG126850.021.88884482-83
CCACGAG131350.021.54763884-85
ATCTCCG127750.021.29560774-75
TCTCCGA127450.020.83932776-77
GTACATG292050.020.77181
Produced by FastQC (version 0.11.5)