FastQCFastQC Report
Fri 27 May 2016
SRR2139932_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139932_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10792087
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114750.045.4909131
TATCTCG145700.043.8298394-95
CCGTCTT61400.034.06160492-93
GTATCAA197550.033.391231
TATGCCG60850.032.75461288-89
TGCCGTC63700.031.65358590-91
CTATCTC186650.031.06485694-95
GCCGTCT63400.030.44682590-91
ATGCCGT64050.029.24530488-89
CGTATGC69700.028.58705786-87
CGCTATC196050.027.3400292-93
TCAACGC239150.027.0208634
CTCGTAT73000.026.98569984-85
ATCAACG241450.026.7808483
ATCTCGT118200.026.72800894-95
CAACGCA245800.026.2386135
TCGTATG71850.026.0732284-85
AACGCAG255800.025.36056
TATCAAC256750.025.2671342
TCTCGTA74250.024.70913982-83
Produced by FastQC (version 0.11.5)