##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139932_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10792087
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.869533946492464	31.0	31.0	34.0	30.0	34.0
2	32.011687544772386	33.0	31.0	34.0	30.0	34.0
3	32.07990975239544	33.0	31.0	34.0	30.0	34.0
4	35.77257086604287	37.0	35.0	37.0	35.0	37.0
5	35.62846398476958	37.0	35.0	37.0	33.0	37.0
6	35.62596483886759	37.0	35.0	37.0	33.0	37.0
7	35.50336417784623	37.0	35.0	37.0	33.0	37.0
8	35.49893787920724	37.0	35.0	37.0	33.0	37.0
9	37.105365533098464	39.0	37.0	39.0	33.0	39.0
10-11	36.98078318864553	39.0	37.0	39.0	33.0	39.0
12-13	36.94111542095611	39.0	37.0	39.0	33.0	39.0
14-15	38.11211784152593	40.0	37.0	41.0	33.0	41.0
16-17	38.10398512354469	40.0	37.0	41.0	33.0	41.0
18-19	38.12736053740115	40.0	37.0	41.0	33.0	41.0
20-21	38.10812685209565	40.0	37.0	41.0	33.0	41.0
22-23	38.019706754352356	40.0	37.0	41.0	33.0	41.0
24-25	37.92431816312205	40.0	37.0	41.0	33.0	41.0
26-27	37.7581875664102	40.0	37.0	41.0	32.0	41.0
28-29	37.622390372688415	39.5	37.0	41.0	32.0	41.0
30-31	37.48617421772988	39.0	36.0	41.0	32.0	41.0
32-33	37.649362427841325	39.5	37.0	41.0	32.0	41.0
34-35	37.6183220661903	40.0	37.0	41.0	32.0	41.0
36-37	37.49245075197774	39.5	36.0	41.0	31.5	41.0
38-39	37.33644293195228	39.0	36.0	41.0	31.0	41.0
40-41	37.28165856326645	39.0	36.0	41.0	31.0	41.0
42-43	37.169553542524355	39.0	36.0	40.5	31.0	41.0
44-45	37.23299215696463	39.0	36.0	40.5	31.0	41.0
46-47	37.37791227912744	39.0	36.0	41.0	31.0	41.0
48-49	37.299321072800296	39.0	36.0	41.0	31.0	41.0
50-51	37.15684694923683	39.0	36.0	41.0	31.0	41.0
52-53	37.01699230277876	39.0	35.0	41.0	31.0	41.0
54-55	36.892562272565016	39.0	35.0	40.0	31.0	41.0
56-57	36.74328595462274	39.0	35.0	40.0	30.0	41.0
58-59	36.6103068616255	38.0	35.0	40.0	30.0	41.0
60-61	36.37594644371681	38.0	35.0	40.0	30.0	41.0
62-63	36.11529061527547	38.0	34.0	40.0	29.5	41.0
64-65	35.9109364594938	37.5	34.0	40.0	29.0	41.0
66-67	35.652373884650856	37.0	34.0	40.0	29.0	41.0
68-69	35.360082071434405	36.5	34.0	39.0	29.0	41.0
70-71	35.04721902006211	36.0	34.0	39.0	29.0	40.0
72-73	34.71912504077678	35.5	33.0	38.5	29.0	40.0
74-75	34.36990401566858	35.0	33.0	37.5	28.0	40.0
76-77	33.2239809212771	34.5	31.5	36.0	26.5	39.0
78-79	33.569781655152795	35.0	32.0	36.5	27.5	39.0
80-81	33.428875137265415	35.0	32.0	36.0	27.5	38.5
82-83	33.19780170714263	35.0	32.0	36.0	27.0	37.0
84-85	33.0209196119593	34.5	32.0	35.0	27.0	37.0
86-87	32.83217698132147	34.0	32.0	35.0	27.0	36.5
88-89	32.61260442175573	34.0	32.0	35.0	27.0	36.0
90-91	32.50314937612758	34.0	32.0	35.0	27.0	36.0
92-93	32.453707637203074	34.0	32.0	35.0	27.0	35.5
94-95	32.416331524015966	34.0	31.5	35.0	27.0	35.0
96-97	32.30528238292804	34.0	31.0	35.0	27.0	35.0
98-99	32.45636559822821	34.0	31.0	35.0	27.0	35.0
100-101	31.65000485476429	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	2.0
19	11.0
20	97.0
21	564.0
22	2712.0
23	9074.0
24	23030.0
25	48599.0
26	85142.0
27	133378.0
28	187037.0
29	246358.0
30	307610.0
31	377803.0
32	461066.0
33	572051.0
34	740989.0
35	984093.0
36	1418086.0
37	2104504.0
38	2159156.0
39	895597.0
40	35126.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.927419691853856	21.44084828078202	14.65533960206214	24.97639242530198
2	14.5300749092823	21.961735478681803	40.359053051784144	23.149136560251748
3	17.803257145721677	26.07652254841904	30.89621127035021	25.22400903550907
4	12.635832161100998	18.23154316676654	37.93050408136999	31.202120590762473
5	13.092120180276531	37.852465422119	35.81544514976575	13.239969247838717
6	30.86575376940531	37.91564133980758	17.995870492889836	13.222734397897273
7	26.52099635594116	32.46743655791507	23.204538658741352	17.80702842740241
8	24.309783640550712	37.456573506125366	20.87644400939318	17.357198843930743
9	25.39966551418646	16.26419431199915	21.156139679007406	37.18000049480698
10-11	24.446272532828914	26.683036376559976	28.911613666568847	19.959077424042263
12-13	24.823196847838606	24.28369508140548	29.08305409324443	21.810053977511487
14-15	21.94662626422489	25.787903674238354	26.440803340447495	25.824666721089258
16-17	21.79789692206892	28.76467267174551	27.308800420159695	22.12862998602587
18-19	21.828544472284317	28.039842753138633	28.809509347854355	21.32210342672269
20-21	22.473925141095233	27.31972732128875	28.63923400354255	21.567113534073464
22-23	22.259238033030456	27.209946466654632	28.52790065581221	22.0029148445027
24-25	22.04694632087391	27.360670737741337	28.585969999922593	22.006412941462152
26-27	22.056806589561052	27.464167979626747	28.487885007372316	21.991140423439887
28-29	22.001246327123333	27.53303274928433	28.509340888192042	21.956380035400294
30-31	22.214132062534024	27.41594523085783	28.394286197730718	21.975636508877425
32-33	22.02251214627253	27.59080450794568	28.334375972159563	22.052307373622227
34-35	22.19188878647989	27.56482345284003	28.344372378757793	21.898915381922286
36-37	22.14529393348192	27.5148685107994	28.312245410110343	22.02759214560833
38-39	21.998394583067	27.58139865512781	28.397162337603522	22.023044424201668
40-41	22.16721375022141	27.382149763020518	28.317952295140476	22.132684191617592
42-43	22.21595106749221	27.511198410457	28.08612210606563	22.186728415985165
44-45	22.149869878448968	27.527618656149457	27.981276366441403	22.341235098960173
46-47	22.19232831176548	27.511776990161763	27.80894883258691	22.48694586548585
48-49	22.083083639589002	27.7942567921997	27.795235366489486	22.32742420172181
50-51	22.104294670056547	27.871608310655905	27.700068250628675	22.324028768658877
52-53	22.210342804184187	27.69360491666244	27.3964525062587	22.69959977289467
54-55	22.138131804502255	27.382355462248043	27.640797352411482	22.838715380838217
56-57	22.133106275235896	27.08727703054975	27.965473247220945	22.81414344699341
58-59	21.947971087766604	26.870035187731677	28.136080125770498	23.04591359873122
60-61	22.03430704186549	26.609325551434367	28.47768667318708	22.878680733513065
62-63	21.716953635374672	27.22006189040693	28.462272370866927	22.600712103351466
64-65	21.29473036558564	27.50091703835263	28.249520950895974	22.954831645165758
66-67	21.37693545239843	28.169605538542157	27.67337298635769	22.78008602270172
68-69	21.501188801030167	28.534331468891576	27.123944689388512	22.840535040689744
70-71	21.47866811972022	28.127917958786853	27.083624047854173	23.30978987363876
72-73	21.385553306424704	27.61096677755985	27.06844780976954	23.935032106245906
74-75	21.869033209139698	27.21410766793876	27.11270949378098	23.804149629140568
76-77	22.37467201124342	27.036017747705156	26.996161319948015	23.593148921103406
78-79	22.608880417286812	26.873529155610644	26.2377779826631	24.279812444439443
80-81	22.503423597021506	27.350521812360917	26.11712997536804	24.028924615249533
82-83	22.41231316880613	27.50148451869365	26.203234360218957	23.882967952281263
84-85	22.38823234621504	27.526380990487738	26.211419661242903	23.87396700205432
86-87	22.764861665696152	27.88624994819132	26.15071662512755	23.19817176098498
88-89	22.996165177601146	27.48332076700506	26.523892381508148	22.99662167388565
90-91	22.640558207916012	27.499259079032	26.873533526512027	22.986649186539957
92-93	22.36601765541279	26.86619933334861	26.793759975562566	23.974023035676037
94-95	22.15681511568292	26.86092302949537	27.593428355523674	23.388833499298038
96-97	21.62081633205596	27.223087405649256	27.92497176951288	23.23112449278191
98-99	21.859620650651387	27.23272443604151	28.34876270631364	22.558892206993463
100-101	25.69803183723257	34.925769553565125	29.49192172147159	9.884276887730715
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2314.0
1	2119.5
2	2790.5
3	3893.5
4	5297.0
5	8280.5
6	10807.5
7	12856.0
8	14367.5
9	14528.0
10	13767.5
11	12774.0
12	13698.5
13	17610.5
14	27253.0
15	40648.5
16	50795.0
17	55460.5
18	56292.5
19	54827.5
20	52392.5
21	50176.0
22	49251.5
23	52675.0
24	61574.0
25	74946.5
26	92368.0
27	111353.5
28	131644.0
29	154058.5
30	175992.5
31	198398.0
32	222235.0
33	245468.5
34	267490.0
35	288540.5
36	312560.5
37	335675.0
38	355957.0
39	374890.5
40	391884.5
41	408289.5
42	421651.5
43	434229.5
44	447054.0
45	457525.5
46	464355.5
47	468759.0
48	470346.0
49	466042.5
50	458270.5
51	447732.0
52	432012.5
53	411541.5
54	388062.5
55	360684.0
56	329610.5
57	296324.0
58	262118.5
59	226760.5
60	190671.0
61	156345.5
62	124729.5
63	97120.0
64	73001.5
65	54669.5
66	41607.0
67	31682.0
68	24185.5
69	17787.0
70	12905.0
71	9506.5
72	6884.0
73	4917.5
74	3419.5
75	2334.5
76	1610.0
77	1094.0
78	777.0
79	549.5
80	383.5
81	275.5
82	200.0
83	141.5
84	106.0
85	77.5
86	64.5
87	57.5
88	38.5
89	21.0
90	13.0
91	9.5
92	7.0
93	3.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003400639746510568
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	1.0655955608956821E-4
20-21	0.001899628011136268
22-23	3.150972937034849E-4
24-25	3.708026472898799E-5
26-27	3.014114774893852E-4
28-29	0.003224515747328616
30-31	4.1813437640205684E-4
32-33	6.988711460517694E-5
34-35	0.0023246120002739018
36-37	0.055854542991393204
38-39	0.03337853016710882
40-41	0.04128301038266281
42-43	9.135000108658423E-4
44-45	0.002185318293551961
46-47	0.0015079900138641753
48-49	6.209189172783504E-4
50-51	1.0038332376011037E-5
52-53	9.663317374356258E-4
54-55	0.0021137195299979887
56-57	0.001254383422556221
58-59	0.0033480958239272027
60-61	0.0014567208243097573
62-63	1.448476975604906E-4
64-65	7.332063280314835E-4
66-67	3.4503235421467376E-4
68-69	1.776817984973181E-4
70-71	0.001207463743169293
72-73	0.0013582193112841303
74-75	7.763448402265443E-4
76-77	0.0012508524645220543
78-79	1.856729527598689E-4
80-81	0.002271725112056529
82-83	0.002262586960309243
84-85	0.0040712432160697285
86-87	0.0038214238090734645
88-89	0.06839592949497954
90-91	6.470883612098999E-4
92-93	7.947989416987158E-5
94-95	4.081376387047262E-5
96-97	0.008572759605156742
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	313.0
20-21	1035.0
22-23	2066.0
24-25	4688.0
26-27	5584.0
28-29	9847.0
30-31	28469.0
32-33	37953.0
34-35	54265.0
36-37	78397.0
38-39	57539.0
40-41	89282.0
42-43	98374.0
44-45	115597.0
46-47	115662.0
48-49	106069.0
50-51	99358.0
52-53	18870.0
54-55	18650.0
56-57	18684.0
58-59	19992.0
60-61	117094.0
62-63	116864.0
64-65	125116.0
66-67	132555.0
68-69	134744.0
70-71	133385.0
72-73	130641.0
74-75	134137.0
76-77	133862.0
78-79	143105.0
80-81	144199.0
82-83	138929.0
84-85	142693.0
86-87	152522.0
88-89	162009.0
90-91	174966.0
92-93	189081.0
94-95	245705.0
96-97	304498.0
98-99	1018698.0
100-101	5836590.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.99544468121272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.52239531945334	77.84513639104918
2	6.6481194585537455	11.43415978264305
3	1.5714312140850082	4.054077781235348
4	0.5856958387313924	2.0146869639856777
5	0.25888190460646415	1.113133225327609
6	0.1331590550195046	0.6870643289839421
7	0.08096922723459157	0.4874089291073003
8	0.048696140963452705	0.3350117037128907
9	0.0329752936901962	0.25521525399438094
>10	0.114595776154527	1.5493351423954789
>50	0.0025751231049991204	0.14824760745164173
>100	5.05648402790719E-4	0.07652289011349842
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.266048355614628E-6	9.266048355614628E-6
2	0.0	2.7798145066843883E-5	0.0	9.266048355614628E-6	1.8532096711229257E-5
3	0.0	2.7798145066843883E-5	0.0	1.8532096711229257E-5	1.8532096711229257E-5
4	0.0	6.48623384893024E-5	0.0	2.7798145066843883E-5	1.8532096711229257E-5
5	0.0	6.48623384893024E-5	0.0	3.706419342245851E-5	1.8532096711229257E-5
6	4.633024177807314E-5	6.48623384893024E-5	0.0	3.706419342245851E-5	2.7798145066843883E-5
7	4.633024177807314E-5	7.412838684491703E-5	0.0	3.706419342245851E-5	2.7798145066843883E-5
8	4.633024177807314E-5	1.019265319117609E-4	0.0	3.706419342245851E-5	2.7798145066843883E-5
9	4.633024177807314E-5	1.389907253342194E-4	0.0	4.633024177807314E-5	2.7798145066843883E-5
10-11	5.559629013368777E-5	1.389907253342194E-4	0.0	8.339443520053165E-5	2.7798145066843883E-5
12-13	5.559629013368777E-5	1.389907253342194E-4	0.0	1.1119258026737553E-4	4.169721760026583E-5
14-15	5.559629013368777E-5	1.667888704010633E-4	0.0	1.4825677368983405E-4	5.096326595588045E-5
16-17	8.802745937833896E-5	1.7605491875667792E-4	0.0	1.8995399129009987E-4	5.559629013368777E-5
18-19	9.266048355614628E-5	2.1311911217913643E-4	0.0	2.2238516053475107E-4	5.559629013368777E-5
20-21	9.266048355614628E-5	2.4091725724598032E-4	0.0	3.150456440908974E-4	5.559629013368777E-5
22-23	9.266048355614628E-5	2.9651354737966805E-4	0.0	6.022931431149507E-4	7.876141102272435E-5
24-25	9.266048355614628E-5	3.24311692446512E-4	0.0	0.001464035640187111	1.0655955608956821E-4
26-27	1.019265319117609E-4	3.335777408021266E-4	0.0	0.004058529179759207	1.1119258026737553E-4
28-29	1.019265319117609E-4	3.335777408021266E-4	0.0	0.02152503033009278	1.1582560444518285E-4
30-31	1.019265319117609E-4	3.7990798258019973E-4	0.0	0.035118323267779444	1.2045862862299016E-4
32-33	1.019265319117609E-4	3.7990798258019973E-4	0.0	0.05073624777116789	1.2045862862299016E-4
34-35	1.019265319117609E-4	4.3550427271388747E-4	0.0	0.07133930628987702	1.2045862862299016E-4
36-37	1.019265319117609E-4	4.911005628475753E-4	0.0	0.09820621349698164	1.297246769786048E-4
38-39	1.019265319117609E-4	4.911005628475753E-4	0.0	0.1566564465241987	1.297246769786048E-4
40-41	1.019265319117609E-4	5.652289496924923E-4	0.0	0.486296116775189	1.297246769786048E-4
42-43	1.019265319117609E-4	5.976601189371434E-4	0.0	0.5378616758741845	1.343577011564121E-4
44-45	1.0655955608956821E-4	6.949536266710971E-4	0.0	0.6273856020619553	1.4825677368983403E-4
46-47	1.1119258026737553E-4	7.644489893382068E-4	0.0	0.7329027277115168	1.667888704010633E-4
48-49	1.2045862862299016E-4	7.829810860494361E-4	0.0	0.8207772972919881	1.9458701546790717E-4
50-51	1.389907253342194E-4	8.478434245387384E-4	0.0	0.90786888578641	1.992200396457145E-4
52-53	1.389907253342194E-4	8.617424970721603E-4	0.0	1.1610914552486464	2.316512088903657E-4
54-55	1.389907253342194E-4	8.617424970721603E-4	0.0	2.005789056370654	2.594493539572096E-4
56-57	1.389907253342194E-4	8.71008545427775E-4	0.0	2.9372215031253917	2.594493539572096E-4
58-59	1.389907253342194E-4	8.941736663168116E-4	0.0	3.8868802669956235	2.594493539572096E-4
60-61	1.667888704010633E-4	9.127057630280408E-4	0.0	4.601135072391466	2.733484264906315E-4
62-63	1.8995399129009987E-4	9.219718113836554E-4	0.0	4.621599140184841	2.965135473796681E-4
64-65	1.9458701546790717E-4	0.001019265319117609	0.0	4.634599406027768	2.965135473796681E-4
66-67	2.038530638235218E-4	0.001033164391651031	0.0	4.645218297443303	2.965135473796681E-4
68-69	2.038530638235218E-4	0.0010794946334291042	0.0	4.655058840796965	2.965135473796681E-4
70-71	2.316512088903657E-4	0.001093393705962526	0.0	4.664422182660314	3.335777408021266E-4
72-73	2.4091725724598032E-4	0.001093393705962526	0.0	4.672284424690053	3.6137588586897045E-4
74-75	2.4091725724598032E-4	0.0011165588268515626	0.0	4.680373684904504	3.6137588586897045E-4
76-77	2.455502814237876E-4	0.001167522092807443	0.0	4.688226660885888	3.6600891004677776E-4
78-79	2.5018330560159493E-4	0.0011860541895186724	0.0	4.695194729249311	3.706419342245851E-4
80-81	2.5018330560159493E-4	0.0011860541895186724	0.0	4.699943579031563	3.891740309358144E-4
82-83	2.5018330560159493E-4	0.001195320237874287	0.0	4.703914080751943	4.1697217600265824E-4
84-85	2.5018330560159493E-4	0.0012138523345855163	0.0	4.707458344247966	4.1697217600265824E-4
86-87	2.5018330560159493E-4	0.0012231183829411309	0.0	4.708014307149303	4.2160520018046555E-4
88-89	2.5018330560159493E-4	0.0012972467697860478	0.0	4.708051371342726	4.2623822435827285E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11475	0.0	45.490913	1
TATCTCG	14570	0.0	43.82983	94-95
CCGTCTT	6140	0.0	34.061604	92-93
GTATCAA	19755	0.0	33.39123	1
TATGCCG	6085	0.0	32.754612	88-89
TGCCGTC	6370	0.0	31.653585	90-91
CTATCTC	18665	0.0	31.064856	94-95
GCCGTCT	6340	0.0	30.446825	90-91
ATGCCGT	6405	0.0	29.245304	88-89
CGTATGC	6970	0.0	28.587057	86-87
CGCTATC	19605	0.0	27.34002	92-93
TCAACGC	23915	0.0	27.020863	4
CTCGTAT	7300	0.0	26.985699	84-85
ATCAACG	24145	0.0	26.780848	3
ATCTCGT	11820	0.0	26.728008	94-95
CAACGCA	24580	0.0	26.238613	5
TCGTATG	7185	0.0	26.07322	84-85
AACGCAG	25580	0.0	25.3605	6
TATCAAC	25675	0.0	25.267134	2
TCTCGTA	7425	0.0	24.709139	82-83
>>END_MODULE