FastQCFastQC Report
Fri 27 May 2016
SRR2139931_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139931_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11802954
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122750.044.427261
GTATCAA202950.030.477991
TCAACGC243050.024.5694164
ATCAACG245600.024.3980453
CAACGCA248200.024.0264765
AACGCAG259850.022.9809346
TATCAAC263000.022.8778972
GTACATG289100.020.1154081
TACATGG286300.019.6369782
ACGCAGA306400.019.4224347
CGCAGAG311600.019.1643038
ACATGGG290250.018.5335833
CATGGGG217100.016.4241564
AGAGTAC327500.016.18068710-11
GTGGTAT68150.016.1732181
GAGTACT220950.016.14727812-13
TGGTATC62750.015.9264892
GCAGAGT373600.015.7527579
CAGAGTA364150.015.3596910-11
GTACTTT254400.014.10568714-15
Produced by FastQC (version 0.11.5)