FastQCFastQC Report
Fri 27 May 2016
SRR2139930_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139930_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9805386
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104650.043.6472631
GTATCAA174300.031.8682291
TCAACGC213200.025.4024624
ATCAACG215550.025.1065983
CAACGCA216850.024.9372775
AACGCAG225300.024.0743946
TATCAAC229500.023.9899462
GTACATG247150.020.4615841
ACGCAGA268350.020.075497
CGCAGAG273700.019.7128758
TACATGG252350.019.6197552
ACATGGG256100.018.917853
CATGGGG198050.016.6791954
GAGTACT186850.016.47879412-13
AGAGTAC289800.016.45057510-11
GCAGAGT331500.016.1035279
GTGGTAT52250.015.8448441
CAGAGTA315850.015.44887110-11
AGTACTT203600.014.69243912-13
GTACTTT211600.014.53339614-15
Produced by FastQC (version 0.11.5)