FastQCFastQC Report
Fri 27 May 2016
SRR2139929_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139929_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6611626
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69050.045.185271
GTATCAA115200.031.274811
TCAACGC139600.025.0522174
ATCAACG142750.024.5846673
CAACGCA144250.024.3290215
TATCAAC149350.023.7160952
AACGCAG149900.023.4932126
GTACATG171200.021.1158281
TACATGG173200.020.4503422
ACATGGG176600.019.8034523
ACGCAGA180950.019.461917
CGCAGAG185200.018.993398
CATGGGG134550.018.0620694
GTGGTAT36000.017.4688131
TGGTATC33750.016.9503842
GAGTACT124600.016.29710212-13
AGAGTAC189550.016.021110-11
GCAGAGT218150.015.8827989
CAGAGTA209550.015.63432710-11
GTACTTT136950.014.5772314-15
Produced by FastQC (version 0.11.5)