##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139929_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6611626
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.847907610019078	31.0	31.0	34.0	30.0	34.0
2	31.98259550676339	33.0	31.0	34.0	30.0	34.0
3	32.048946658507305	33.0	31.0	34.0	30.0	34.0
4	35.74938056084842	37.0	35.0	37.0	35.0	37.0
5	35.604169231592955	37.0	35.0	37.0	33.0	37.0
6	35.601672417647336	37.0	35.0	37.0	33.0	37.0
7	35.47390203257111	37.0	35.0	37.0	33.0	37.0
8	35.471837487480386	37.0	35.0	37.0	33.0	37.0
9	37.06998021364185	39.0	37.0	39.0	33.0	39.0
10-11	36.949108056021316	39.0	37.0	39.0	33.0	39.0
12-13	36.905670329809944	39.0	37.0	39.0	33.0	39.0
14-15	38.067437268835235	40.0	37.0	41.0	33.0	41.0
16-17	38.06052618523794	40.0	37.0	41.0	33.0	41.0
18-19	38.07829178480452	40.0	37.0	41.0	33.0	41.0
20-21	38.05774054587921	40.0	37.0	41.0	33.0	41.0
22-23	37.96763274901685	40.0	37.0	41.0	33.0	41.0
24-25	37.86964744577996	40.0	37.0	41.0	32.5	41.0
26-27	37.699041232674645	40.0	37.0	41.0	32.0	41.0
28-29	37.563855839682205	39.5	36.5	41.0	32.0	41.0
30-31	37.427132927656416	39.0	36.0	41.0	32.0	41.0
32-33	37.59491040514631	39.5	37.0	41.0	32.0	41.0
34-35	37.563957336813594	40.0	36.0	41.0	32.0	41.0
36-37	37.43171065813516	39.5	36.0	41.0	31.5	41.0
38-39	37.2761927114766	39.0	36.0	41.0	31.0	41.0
40-41	37.21877994346161	39.0	36.0	41.0	31.0	41.0
42-43	37.10613283112702	39.0	36.0	40.0	31.0	41.0
44-45	37.16973405609939	39.0	36.0	40.5	31.0	41.0
46-47	37.31817699472836	39.0	36.0	41.0	31.0	41.0
48-49	37.24163084583829	39.0	36.0	41.0	31.0	41.0
50-51	37.09786265547005	39.0	35.5	41.0	31.0	41.0
52-53	36.9542237773873	39.0	35.0	40.5	31.0	41.0
54-55	36.819210467637234	39.0	35.0	40.0	31.0	41.0
56-57	36.663576883124115	38.5	35.0	40.0	30.0	41.0
58-59	36.51257731791029	38.0	35.0	40.0	30.0	41.0
60-61	36.26586885824537	38.0	34.5	40.0	30.0	41.0
62-63	35.99146846773168	38.0	34.0	40.0	29.0	41.0
64-65	35.751691742406024	37.0	34.0	40.0	29.0	41.0
66-67	35.480713165988426	37.0	34.0	39.5	29.0	41.0
68-69	35.185684351697546	36.0	34.0	39.0	29.0	40.5
70-71	34.87328674071965	36.0	33.5	39.0	28.5	40.0
72-73	34.54685994162156	35.0	33.0	38.0	28.0	40.0
74-75	34.21504771159167	35.0	33.0	37.5	28.0	39.5
76-77	33.110794294661204	34.0	31.5	36.0	26.5	39.0
78-79	33.50217088892262	35.0	32.0	36.0	27.5	39.0
80-81	33.4002066868884	35.0	32.0	36.0	28.0	38.5
82-83	33.191126358541354	35.0	32.0	36.0	27.0	37.0
84-85	32.999576761860226	34.5	32.0	35.0	27.0	37.0
86-87	32.79957365926826	34.0	32.0	35.0	27.0	36.5
88-89	32.590016917033736	34.0	32.0	35.0	27.0	36.0
90-91	32.466668315177884	34.0	32.0	35.0	27.0	36.0
92-93	32.40441927005213	34.0	31.0	35.0	27.0	35.5
94-95	32.37614278837812	34.0	31.0	35.0	27.0	35.0
96-97	32.285939130851226	34.0	31.0	35.0	27.0	35.0
98-99	32.446246565527005	34.0	31.0	35.0	27.0	35.0
100-101	31.66196200452593	33.0	30.0	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	2.0
19	14.0
20	81.0
21	392.0
22	1825.0
23	5666.0
24	14507.0
25	29696.0
26	52607.0
27	81436.0
28	114355.0
29	150144.0
30	188088.0
31	231989.0
32	284491.0
33	354843.0
34	457326.0
35	608063.0
36	870636.0
37	1266809.0
38	1259620.0
39	616633.0
40	22401.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.36124033634086	21.339259056698005	14.19767240312746	25.10182820383367
2	14.949429192474902	22.28447642814565	39.11099017563995	23.655104203739498
3	17.910722717830684	26.070682158972698	30.85330295452284	25.165292168673787
4	12.782680085050183	18.062757935793705	37.68340798466217	31.47115399449394
5	13.136632350347705	37.85888675493744	35.73803176404715	13.266449130667707
6	31.10593840394759	37.73430888775452	17.84393397992173	13.315818728376156
7	26.741213129720283	32.32818976753978	23.08159898941652	17.848998113323407
8	24.90059177575985	36.70879145311608	20.884605390565046	17.506011380559034
9	25.57659795033778	15.885245172670082	20.96984917174686	37.568307705245275
10-11	24.643710941907482	26.488378199250835	28.79634298733776	20.071567871503923
12-13	24.96519010603443	24.08069512703834	28.967768291793877	21.98634647513335
14-15	22.125820486518748	25.559491719586074	26.247832227654737	26.066855566240438
16-17	22.029006177905405	28.56653567518792	27.107711476722972	22.296746670183705
18-19	22.06601240951443	27.863479157643727	28.56902479505592	21.501483637785928
20-21	22.676464624562414	27.088374251477305	28.44901658277878	21.7861445411815
22-23	22.454972309183198	27.02919314544216	28.298964155673072	22.21687038970157
24-25	22.264299663679743	27.137652222519144	28.404837830566375	22.19321028323474
26-27	22.278737361870757	27.230672200717958	28.298600177309257	22.191990260102028
28-29	22.215368145243335	27.315182713969037	28.322555269928333	22.14689387085929
30-31	22.39520638250704	27.220772524556303	28.181151495774902	22.202869597161754
32-33	22.263717939117427	27.388611472847828	28.143890342938583	22.20378024509616
34-35	22.378150492992706	27.408498941253217	28.132191825580595	22.081158740173485
36-37	22.36276749725569	27.33093317670903	28.117056041825585	22.1892432842097
38-39	22.24037390464658	27.422536052079977	28.146321881652188	22.190768161621254
40-41	22.46258103018792	27.271880158982377	28.033633479246124	22.231905331583583
42-43	22.332003050382745	27.44275507986999	27.80915390174748	22.416087967999783
44-45	22.426942320489434	27.473392181911926	27.709081611717696	22.390583885880943
46-47	22.49590535301576	27.4604346369413	27.49161804062117	22.55204196942177
48-49	22.39203387433006	27.601970210782508	27.45910387415006	22.546892040737372
50-51	22.472352115706514	27.66926742071727	27.369113212592715	22.4892672509835
52-53	22.606371896061383	27.533881945671535	27.27020253444387	22.58954362382321
54-55	22.497354598354445	27.46487221071722	27.34910260016756	22.688670590760776
56-57	22.52411773418308	27.574693619169306	27.322895881255377	22.578292765392234
58-59	22.511504464760236	27.60026119806136	27.33444016216134	22.55379417501706
60-61	22.61045831254184	27.555515501721622	27.314807744556774	22.51921844117976
62-63	22.613446907608743	27.67285611182731	27.23078631931171	22.48291066125223
64-65	22.56428262541412	27.59503091440222	27.240689490381044	22.59999696980262
66-67	22.578591742494037	27.572695456528756	27.150351446811534	22.698361354165673
68-69	22.512539245893297	27.533500043163308	27.23931176322899	22.714648947714412
70-71	22.52462103780064	27.382648825077016	27.28590982214222	22.80682031498013
72-73	22.57289400597156	27.438574799412585	27.181982764412847	22.80654843020301
74-75	22.409412196169225	27.531347607757183	27.24460174207277	22.814638454000818
76-77	22.62159099003538	27.406233357006716	27.208926134923416	22.76324951803449
78-79	22.53268460589665	27.406573864950172	27.153170673065617	22.907570856087556
80-81	22.539263060867963	27.47806733731681	27.16499569837548	22.817673903439747
82-83	22.550916615228243	27.393175589301897	27.279058131059834	22.776849664410022
84-85	22.564015438407186	27.366794455453764	27.24316658539369	22.82602352074536
86-87	22.51598310051705	27.358035669314066	27.301429256808284	22.8245519733606
88-89	22.540104572129646	27.286735157168014	27.295049554030626	22.878110716671717
90-91	22.523068088623678	27.34587304560987	27.32335972484436	22.807699140922097
92-93	22.55162328871305	27.22395948872324	27.41395507920876	22.81046214335496
94-95	22.3900938896237	27.248953046433645	27.461638857741498	22.899314206201154
96-97	22.300083299019995	27.32715453350844	27.541936454098227	22.830825713373336
98-99	22.492533652126035	27.93213496843138	27.59570743913909	21.979623940303497
100-101	26.19271472578414	35.266957144936114	28.515468135832922	10.024859993446821
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1626.0
1	1430.5
2	1828.5
3	2576.0
4	3396.0
5	5151.0
6	6655.0
7	7935.5
8	8902.0
9	9001.5
10	8488.0
11	7842.5
12	8527.0
13	11118.5
14	16882.5
15	24589.5
16	30509.0
17	33156.0
18	33790.0
19	33532.0
20	32708.0
21	31955.5
22	32268.5
23	35273.0
24	41598.5
25	51190.5
26	62648.5
27	74353.5
28	87415.5
29	102013.5
30	115934.5
31	129757.0
32	143406.5
33	156211.0
34	168831.5
35	181335.0
36	195442.0
37	208959.0
38	220471.0
39	230578.5
40	240574.5
41	251183.0
42	259154.5
43	266700.0
44	274555.0
45	281250.0
46	286132.5
47	288624.5
48	289562.5
49	288424.0
50	286412.5
51	282895.0
52	274791.0
53	262912.5
54	250450.5
55	236395.5
56	220197.0
57	201853.0
58	181412.5
59	160038.0
60	137380.5
61	114504.5
62	93067.0
63	73716.5
64	56430.5
65	42733.5
66	32447.0
67	24681.0
68	18872.5
69	13823.0
70	9974.0
71	7322.5
72	5286.5
73	3741.0
74	2576.0
75	1746.5
76	1187.5
77	790.0
78	534.0
79	380.5
80	267.0
81	182.0
82	136.0
83	104.0
84	75.0
85	52.0
86	37.5
87	30.5
88	20.5
89	11.0
90	6.5
91	5.5
92	5.0
93	3.5
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0030703491092811362
3	0.0
4	0.0
5	0.0
6	1.5124872459512987E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	8.318679852732143E-5
20-21	0.002049539564508388
22-23	3.7819025223701424E-4
24-25	3.0263669191463705E-5
26-27	3.557678253116394E-4
28-29	0.0038936172856003128
30-31	3.641723038769025E-4
32-33	4.566099541883233E-5
34-35	0.002254634611621992
36-37	0.05607070354196096
38-39	0.031717428521317495
40-41	0.04197328327665478
42-43	8.387057978307586E-4
44-45	0.002145277727414448
46-47	0.0014601796832221297
48-49	6.410943974514032E-4
50-51	8.32028227722465E-6
52-53	8.755002452663427E-4
54-55	0.0024116311864305095
56-57	0.001096788379086681
58-59	0.0036947408603317912
60-61	0.0012874280871526663
62-63	9.001250093613001E-5
64-65	7.392896776998197E-4
66-67	4.8181879925381094E-4
68-69	2.3535007004959484E-4
70-71	0.0012246504531876407
72-73	0.001575173553497221
74-75	7.805101671329144E-4
76-77	0.0012357293354910708
78-79	2.044476774437171E-4
80-81	0.002103134159240365
82-83	0.002927634911197726
84-85	0.004074290416933918
86-87	0.004221055462244147
88-89	0.06951992970324841
90-91	8.525954618878308E-4
92-93	1.1818572153387575E-4
94-95	9.727217215812661E-5
96-97	0.008269872060660205
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	270.0
20-21	670.0
22-23	1296.0
24-25	3029.0
26-27	4234.0
28-29	7697.0
30-31	18529.0
32-33	25563.0
34-35	36929.0
36-37	55466.0
38-39	60321.0
40-41	61916.0
42-43	69715.0
44-45	81536.0
46-47	81937.0
48-49	75217.0
50-51	70453.0
52-53	70714.0
54-55	76696.0
56-57	78818.0
58-59	79452.0
60-61	77662.0
62-63	75578.0
64-65	80509.0
66-67	85128.0
68-69	84881.0
70-71	84828.0
72-73	81209.0
74-75	83946.0
76-77	83941.0
78-79	88790.0
80-81	89195.0
82-83	86938.0
84-85	88787.0
86-87	94012.0
88-89	99694.0
90-91	108001.0
92-93	113470.0
94-95	144671.0
96-97	179329.0
98-99	533754.0
100-101	3286845.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.5072695934333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.13029643291381	81.54200984110828
2	5.682328739221098	10.058548032815114
3	1.2442081871650075	3.3036440835531073
4	0.43858956396299975	1.552734591141583
5	0.19184203422856022	0.8489707321409921
6	0.10367906574840063	0.5505810614033396
7	0.05710287556222273	0.3537813721362145
8	0.03686466162171341	0.26102324356989465
9	0.027676496567069962	0.22046140287570537
>10	0.08546827845377752	1.1719994693531008
>50	0.0016537542463720086	0.09473721641156965
>100	2.89910309000333E-4	0.041508953491167726
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.024974491902597E-5
2	0.0	4.5374617378538954E-5	0.0	1.5124872459512985E-5	3.024974491902597E-5
3	0.0	4.5374617378538954E-5	0.0	1.5124872459512985E-5	3.024974491902597E-5
4	0.0	1.2099897967610388E-4	0.0	1.5124872459512985E-5	3.024974491902597E-5
5	0.0	1.2099897967610388E-4	0.0	3.024974491902597E-5	3.024974491902597E-5
6	0.0	1.2099897967610388E-4	0.0	3.024974491902597E-5	3.024974491902597E-5
7	0.0	1.2099897967610388E-4	0.0	4.5374617378538954E-5	3.024974491902597E-5
8	0.0	1.3612385213561685E-4	0.0	4.5374617378538954E-5	6.049948983805194E-5
9	0.0	2.4199795935220776E-4	0.0	6.049948983805194E-5	6.049948983805194E-5
10-11	0.0	2.4199795935220776E-4	0.0	6.049948983805194E-5	6.049948983805194E-5
12-13	0.0	2.4199795935220776E-4	0.0	6.049948983805194E-5	6.049948983805194E-5
14-15	0.0	3.024974491902597E-4	0.0	6.049948983805194E-5	8.318679852732141E-5
16-17	0.0	3.1762232164977267E-4	0.0	7.562436229756492E-5	9.074923475707791E-5
18-19	0.0	3.3274719410928566E-4	0.0	1.4368628836537335E-4	9.074923475707791E-5
20-21	1.5124872459512985E-5	3.4787206656879864E-4	0.0	1.7393603328439932E-4	9.074923475707791E-5
22-23	3.024974491902597E-5	3.705593752580681E-4	0.0	2.041857782034253E-4	9.83116709868344E-5
24-25	3.024974491902597E-5	3.932466839473376E-4	0.0	2.4956039558196423E-4	1.058741072165909E-4
26-27	3.024974491902597E-5	3.932466839473376E-4	0.0	3.4787206656879864E-4	1.058741072165909E-4
28-29	3.024974491902597E-5	4.159339926366071E-4	0.0	7.033065693673537E-4	1.058741072165909E-4
30-31	3.024974491902597E-5	5.293705360829544E-4	0.0	0.001966233419736688	1.058741072165909E-4
32-33	3.024974491902597E-5	5.596202810019804E-4	0.0	0.004295463778501688	1.3612385213561685E-4
34-35	3.024974491902597E-5	6.881816969078408E-4	0.0	0.007834683934027725	1.3612385213561685E-4
36-37	3.024974491902597E-5	8.167431128137012E-4	0.0	0.013037640060100192	1.3612385213561685E-4
38-39	3.024974491902597E-5	8.243055490434576E-4	0.0	0.0210840722085611	1.3612385213561685E-4
40-41	3.024974491902597E-5	9.226172200302921E-4	0.0	0.029531313477199103	1.5124872459512984E-4
42-43	3.024974491902597E-5	9.528669649493181E-4	0.0	0.03770630704156587	1.5124872459512984E-4
44-45	3.781218114878246E-5	0.001134365434463474	0.0	0.04783240915320981	1.5124872459512984E-4
46-47	4.5374617378538954E-5	0.0012704892865990907	0.0	0.05941806145719676	1.6637359705464283E-4
48-49	4.5374617378538954E-5	0.0012780517228288472	0.0	0.06960466305867877	1.966233419736688E-4
50-51	4.5374617378538954E-5	0.0013536760851264123	0.0	0.0800105753108237	1.966233419736688E-4
52-53	4.5374617378538954E-5	0.0013612385213561686	0.0	0.08987199215442615	2.117482144331818E-4
54-55	5.293705360829545E-5	0.001383925830045438	0.0	0.10076946276150527	2.2687308689269478E-4
56-57	6.049948983805194E-5	0.0014066131387347077	0.0	0.11200724299892342	2.2687308689269478E-4
58-59	7.562436229756492E-5	0.0014217380111942205	0.0	0.12322233592765228	2.2687308689269478E-4
60-61	7.562436229756492E-5	0.001489799937262029	0.0	0.13363581061602697	2.2687308689269478E-4
62-63	7.562436229756492E-5	0.0015124872459512985	0.0	0.14336110360749382	2.2687308689269478E-4
64-65	7.562436229756492E-5	0.0016788608430059414	0.0	0.15327545750470462	2.2687308689269478E-4
66-67	7.562436229756492E-5	0.0016788608430059414	0.0	0.16368893219307928	2.2687308689269478E-4
68-69	9.074923475707791E-5	0.0017317978966142368	0.0	0.17473008908852378	2.2687308689269478E-4
70-71	1.058741072165909E-4	0.0017696100777630191	0.0	0.18523431301165552	2.2687308689269478E-4
72-73	1.058741072165909E-4	0.0017998598226820453	0.0	0.19402186391063259	2.571228318117207E-4
74-75	1.058741072165909E-4	0.0018603593125200973	0.0	0.20217417016631006	2.571228318117207E-4
76-77	1.058741072165909E-4	0.001966233419736688	0.0	0.2098349180670534	2.571228318117207E-4
78-79	1.058741072165909E-4	0.00202673290957474	0.0	0.21683773401580791	2.571228318117207E-4
80-81	1.058741072165909E-4	0.00202673290957474	0.0	0.22169281807531158	2.646852680414772E-4
82-83	1.058741072165909E-4	0.00202673290957474	0.0	0.22533034990182443	2.722477042712337E-4
84-85	1.058741072165909E-4	0.0020645450907235225	0.0	0.22862000966176854	2.722477042712337E-4
86-87	1.058741072165909E-4	0.002087232399412792	0.0	0.22907375583555392	2.722477042712337E-4
88-89	1.058741072165909E-4	0.0021174821443318177	0.0	0.2291266928891622	2.722477042712337E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6905	0.0	45.18527	1
GTATCAA	11520	0.0	31.27481	1
TCAACGC	13960	0.0	25.052217	4
ATCAACG	14275	0.0	24.584667	3
CAACGCA	14425	0.0	24.329021	5
TATCAAC	14935	0.0	23.716095	2
AACGCAG	14990	0.0	23.493212	6
GTACATG	17120	0.0	21.115828	1
TACATGG	17320	0.0	20.450342	2
ACATGGG	17660	0.0	19.803452	3
ACGCAGA	18095	0.0	19.46191	7
CGCAGAG	18520	0.0	18.99339	8
CATGGGG	13455	0.0	18.062069	4
GTGGTAT	3600	0.0	17.468813	1
TGGTATC	3375	0.0	16.950384	2
GAGTACT	12460	0.0	16.297102	12-13
AGAGTAC	18955	0.0	16.0211	10-11
GCAGAGT	21815	0.0	15.882798	9
CAGAGTA	20955	0.0	15.634327	10-11
GTACTTT	13695	0.0	14.57723	14-15
>>END_MODULE