FastQCFastQC Report
Fri 27 May 2016
SRR2139928_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139928_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12513615
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA165950.039.39311
GTATCAA269800.028.4323831
CTTATAC126750.024.2655621
TATACAC148850.023.5787873
ATCAACG324200.022.8915233
CAACGCA328800.022.4856765
AACGCAG338750.022.1100436
TCAACGC336900.022.1001914
TATCAAC353000.021.537422
TACATGG369950.020.3658922
GTACATG390800.019.783431
ACATGGG373100.019.6865393
TAACGCC13650.019.1441784
ACGCAGA390200.018.957747
CGCAGAG398750.018.5411688
GGCGTCG17800.018.2945218
GTACTAG39250.018.1305271
CATGGGG270100.017.7273274
ACACATC179950.017.4260436
ACATCTC186550.017.2620878
Produced by FastQC (version 0.11.5)