##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139928_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12513615
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81737755236996	31.0	31.0	34.0	30.0	34.0
2	31.961700276059315	31.0	31.0	34.0	30.0	34.0
3	32.02583945566489	33.0	31.0	34.0	30.0	34.0
4	35.73241657186992	37.0	35.0	37.0	35.0	37.0
5	35.59185167515542	37.0	35.0	37.0	33.0	37.0
6	35.58965654609	37.0	35.0	37.0	33.0	37.0
7	35.461870770356924	37.0	35.0	37.0	33.0	37.0
8	35.45890408167424	37.0	35.0	37.0	33.0	37.0
9	37.048815789841704	39.0	37.0	39.0	33.0	39.0
10-11	36.927153664228925	39.0	37.0	39.0	33.0	39.0
12-13	36.88204779354327	39.0	37.0	39.0	33.0	39.0
14-15	38.02862506158293	40.0	37.0	41.0	33.0	41.0
16-17	38.02264073970631	40.0	37.0	41.0	33.0	41.0
18-19	38.05563140627228	40.0	37.0	41.0	33.0	41.0
20-21	38.04410351291676	40.0	37.0	41.0	33.0	41.0
22-23	37.95183025839617	40.0	37.0	41.0	33.0	41.0
24-25	37.848918072447816	40.0	37.0	41.0	32.0	41.0
26-27	37.6716700098797	40.0	37.0	41.0	32.0	41.0
28-29	37.534530660241	39.0	36.5	41.0	32.0	41.0
30-31	37.390530442664684	39.0	36.0	41.0	31.5	41.0
32-33	37.55088743887714	39.0	36.0	41.0	32.0	41.0
34-35	37.521843726234806	40.0	36.0	41.0	32.0	41.0
36-37	37.39385760227235	39.0	36.0	41.0	31.5	41.0
38-39	37.23066533570092	39.0	36.0	41.0	31.0	41.0
40-41	37.18939538515075	39.0	36.0	40.5	31.0	41.0
42-43	37.08608250137029	39.0	36.0	40.0	31.0	41.0
44-45	37.15372585667636	39.0	36.0	40.5	31.0	41.0
46-47	37.30090812857832	39.0	36.0	41.0	31.0	41.0
48-49	37.223671440992106	39.0	36.0	41.0	31.0	41.0
50-51	37.07881766655959	39.0	35.5	41.0	31.0	41.0
52-53	36.950319009010244	39.0	35.0	40.5	31.0	41.0
54-55	36.82593075798718	39.0	35.0	40.0	31.0	41.0
56-57	36.67727931739576	38.5	35.0	40.0	30.0	41.0
58-59	36.54510477736481	38.0	35.0	40.0	30.0	41.0
60-61	36.31412314957391	38.0	35.0	40.0	30.0	41.0
62-63	36.05932103311798	38.0	34.0	40.0	29.0	41.0
64-65	35.84146232540138	37.0	34.0	40.0	29.0	41.0
66-67	35.59524125783898	37.0	34.0	40.0	29.0	41.0
68-69	35.31293844946434	36.5	34.0	39.0	29.0	41.0
70-71	35.0222470300349	36.0	34.0	39.0	29.0	40.0
72-73	34.706503520510005	35.5	33.0	38.5	28.0	40.0
74-75	34.381235858803244	35.0	33.0	38.0	28.0	40.0
76-77	33.25811546443942	34.5	31.5	36.5	26.5	39.0
78-79	33.65397819947944	35.0	32.0	37.0	28.0	39.0
80-81	33.549896369091286	35.0	32.0	36.0	28.0	39.0
82-83	33.31682944925548	35.0	32.0	36.0	27.5	37.5
84-85	33.11219458041526	35.0	32.0	35.5	27.0	37.0
86-87	32.9048638540266	34.0	32.0	35.0	27.0	37.0
88-89	32.675914329998996	34.0	32.0	35.0	27.0	36.0
90-91	32.5444110370578	34.0	32.0	35.0	27.0	36.0
92-93	32.473074556332605	34.0	32.0	35.0	27.0	36.0
94-95	32.43987244181053	34.0	31.5	35.0	27.0	35.0
96-97	32.343052558429946	34.0	31.0	35.0	27.0	35.0
98-99	32.49285882077275	34.0	31.0	35.0	27.0	35.0
100-101	31.718777535102873	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	3.0
18	3.0
19	19.0
20	147.0
21	740.0
22	3273.0
23	10529.0
24	26983.0
25	55630.0
26	98478.0
27	152126.0
28	214341.0
29	282006.0
30	356039.0
31	436829.0
32	537079.0
33	667559.0
34	862431.0
35	1136926.0
36	1630391.0
37	2371351.0
38	2413764.0
39	1212982.0
40	43985.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.55291216806654	22.36433676439622	14.220974514558742	24.861776552978494
2	14.506328403787142	22.440677982355734	39.99376817432681	23.059225439530312
3	17.312015752442438	27.129930080156694	31.5629816004408	23.99507256696007
4	11.92968618580642	18.643189837628853	39.018756770125975	30.408367206438747
5	12.143389420243471	38.84676810018528	36.529412164270674	12.480430315300575
6	29.47510371703141	39.286648981928884	18.277404251289493	12.960843049750212
7	25.50244673501622	33.063091680541554	23.826839806083214	17.60762177835901
8	24.430238584134162	37.15367621586568	21.258940761722332	17.157144438277825
9	25.17157512037888	15.667886537982827	21.55918173924961	37.60135660238868
10-11	24.043511806939883	27.064353506161087	29.27584075425047	19.61629393264856
12-13	24.493581590931157	24.498979711298453	29.33468466146673	21.672754036303658
14-15	21.605367433791116	26.097298822122944	26.802750444216162	25.494583299869785
16-17	21.283785700614892	29.039270426651292	27.699241985629254	21.977701887104566
18-19	21.072551378296303	28.382761285100905	29.37310770896403	21.17157962763876
20-21	22.02783865348182	27.699890975170437	28.992097338186195	21.280173033161546
22-23	21.675301213365785	27.671102200036007	28.891745423976655	21.761851162621547
24-25	21.45993971099493	27.719746867725952	29.210502646887992	21.609810774391125
26-27	21.588675822066982	27.95245503201548	28.974414051353627	21.484455094563913
28-29	21.365869648877638	28.08042715921551	29.013053843778536	21.540649348128316
30-31	21.651234002443363	27.952242193580428	28.931653183146857	21.464870620829355
32-33	21.387573109227073	28.035617512171353	29.005437051723508	21.57137232687807
34-35	21.44125465371362	28.086913214022154	28.990916144897366	21.48091598736686
36-37	21.503084346030608	27.9989283018303	29.02471805274428	21.473269299394815
38-39	21.4204344366554	28.07803699601004	29.028446215204873	21.473082352129687
40-41	21.58303766266864	27.95804645646507	28.964471569158395	21.494444311707895
42-43	21.617932625950647	28.03237100837763	28.7421580848285	21.607538280843215
44-45	21.55713375045431	28.448339418475694	28.434994987986407	21.55953184308359
46-47	21.797243749360614	28.080976121725705	28.037812290854554	22.083967838059124
48-49	21.85552157201718	28.122799039976442	28.145504542017363	21.876174845989016
50-51	21.60477391145683	28.286601400710282	28.062432184232712	22.046192503600174
52-53	21.662301961557116	28.330702892755927	28.04651585971774	21.960479285969217
54-55	21.759039624144556	28.227432331870826	27.921424437229287	22.092103606755327
56-57	21.989089935700033	28.349887075189866	27.688922189847197	21.972100799262904
58-59	21.67165719119134	28.49936603439332	28.07919056221247	21.74978621220287
60-61	21.926690294639027	28.65237228941663	27.668126143839416	21.75281127210493
62-63	21.89435008170165	28.545338401434435	27.613446349731475	21.946865167132444
64-65	21.717973405755856	28.508480796532854	27.747782874610248	22.02576292310104
66-67	21.939918988807648	28.506657050827027	27.524859779514	22.028564180851323
68-69	21.854017087145333	28.315411932325198	27.753075373775925	22.077495606753548
70-71	21.856798559356193	27.940430031415087	27.89422940710932	22.3085420021194
72-73	21.97493935632095	28.04199846381563	27.96863440330442	22.014427776559
74-75	21.838044585728976	28.219109236919838	27.824806398077754	22.118039779273428
76-77	21.791032319062765	28.286941584848012	27.74476152412731	22.177264571961913
78-79	21.87513685794378	28.202407025151345	27.70054911745184	22.221906999453036
80-81	21.78507757522457	28.090320285125042	27.865923595430935	22.25867854421946
82-83	21.99582707382007	27.960356571786456	28.08553634531259	21.958280009080884
84-85	22.24024000597496	27.948742660512828	27.70028731475533	22.11073001875688
86-87	21.708698096108655	28.159117451166015	27.917318363210196	22.21486608951513
88-89	22.001999741478752	27.929647701898624	27.90612311895253	22.162229437670096
90-91	21.97517201116307	27.89296022310231	28.087672483584598	22.044195282150024
92-93	21.98459441319523	27.88080022536674	27.98108791483606	22.15351744660197
94-95	21.634459684662204	27.971705828658582	28.108180463684523	22.285654022994684
96-97	21.551144160825604	28.0648656819159	28.20701894503051	22.176971212227986
98-99	21.616334269308542	28.681630254948114	28.325294039195615	21.376741436547732
100-101	25.231183914351107	36.06360300376416	29.09941929748236	9.605793784402367
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2319.0
1	2258.5
2	3879.5
3	5887.0
4	8338.5
5	13679.0
6	17827.5
7	20266.0
8	21190.5
9	19809.5
10	17739.0
11	16231.0
12	18320.5
13	25697.5
14	41857.0
15	62985.5
16	77910.0
17	82934.5
18	82163.5
19	79423.5
20	75617.5
21	72665.5
22	73074.5
23	80434.5
24	94960.0
25	116940.0
26	147737.5
27	178821.5
28	204569.5
29	230920.0
30	253185.0
31	272932.0
32	300669.5
33	331840.0
34	364306.5
35	398602.0
36	431585.0
37	458192.0
38	471567.5
39	477193.5
40	489807.0
41	499834.0
42	504249.0
43	517055.0
44	530920.0
45	543697.5
46	552738.0
47	558008.0
48	564482.5
49	553388.0
50	527584.0
51	509601.5
52	490118.5
53	461906.0
54	428294.5
55	392666.5
56	361450.0
57	326689.0
58	288931.5
59	250798.5
60	208099.0
61	163820.5
62	124938.0
63	94938.5
64	69587.0
65	49314.0
66	35158.0
67	25951.5
68	19336.5
69	13937.0
70	10456.5
71	7805.5
72	5527.5
73	3807.0
74	2623.0
75	1752.5
76	1210.5
77	801.5
78	509.5
79	342.5
80	249.0
81	171.5
82	118.0
83	75.5
84	44.5
85	25.5
86	19.0
87	16.5
88	14.0
89	11.0
90	7.0
91	3.0
92	1.5
93	2.5
94	2.5
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0034202746368655265
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	1.1187814232737702E-4
20-21	0.0020099100954827174
22-23	3.59674350842747E-4
24-25	2.3984481081361118E-5
26-27	3.3593674982874223E-4
28-29	0.0033819696591294766
30-31	3.4877616648088655E-4
32-33	5.62962194474888E-5
34-35	0.0022296127962325362
36-37	0.054954115996608106
38-39	0.03205966060266662
40-41	0.040986344146223354
42-43	8.969233475476125E-4
44-45	0.0024065235377950253
46-47	0.0015026384652283215
48-49	5.763671144136753E-4
50-51	8.848900510838179E-6
52-53	9.273017802850266E-4
54-55	0.002237227935725213
56-57	0.0012430920912087054
58-59	0.0037443309147105926
60-61	0.0014528879019591243
62-63	1.5428572723469495E-4
64-65	7.441687766952189E-4
66-67	4.2809152517163233E-4
68-69	1.9745457759959837E-4
70-71	0.0011998011934571803
72-73	0.001743491736451276
74-75	7.184088106933714E-4
76-77	0.0011203954790364535
78-79	2.093319638615908E-4
80-81	0.0023746008649609958
82-83	0.0024435611762422217
84-85	0.004197689486764248
86-87	0.004250983537873023
88-89	0.06834531522135921
90-91	7.272752136837143E-4
92-93	9.564559405064E-5
94-95	5.246751441053076E-5
96-97	0.008496608811432677
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	389.0
20-21	1358.0
22-23	2827.0
24-25	5006.0
26-27	8093.0
28-29	15791.0
30-31	34647.0
32-33	50426.0
34-35	72839.0
36-37	109318.0
38-39	123930.0
40-41	125705.0
42-43	142655.0
44-45	165144.0
46-47	165526.0
48-49	153414.0
50-51	140497.0
52-53	140126.0
54-55	154710.0
56-57	164700.0
58-59	163808.0
60-61	163769.0
62-63	156398.0
64-65	163352.0
66-67	172813.0
68-69	165357.0
70-71	162815.0
72-73	153995.0
74-75	157076.0
76-77	158859.0
78-79	168625.0
80-81	171840.0
82-83	161453.0
84-85	163865.0
86-87	173532.0
88-89	186071.0
90-91	200743.0
92-93	209364.0
94-95	265935.0
96-97	325652.0
98-99	970661.0
100-101	6120531.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.47419268521375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.24889026873528	73.60730172965174
2	6.799742307119999	11.216065144944293
3	1.9712709637330714	4.877369438930651
4	0.8292591588933695	2.7356991862620017
5	0.43594711394325536	1.7977193137959437
6	0.23548045491182198	1.165263624719964
7	0.14171512104071776	0.8181488139384383
8	0.09034570963775573	0.5960951571957313
9	0.06181885138194363	0.45886138744077104
>10	0.18128275447017367	2.1634781197175417
>50	0.0028646919102373217	0.16020262581264672
>100	0.0012290003854605225	0.20593469087740768
>500	6.827174491783267E-5	0.042243246433837194
>1k	8.5332091979292E-5	0.1556175202790394
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	5.593907116368851E-5	0.0
8	0.0	0.0	0.0	5.593907116368851E-5	0.0
9	0.0	0.0	0.0	9.589555056632317E-5	0.0
10-11	0.0	0.0	0.0	9.589555056632317E-5	0.0
12-13	0.0	3.995647940263465E-6	0.0	1.0388684644685009E-4	0.0
14-15	0.0	7.99129588052693E-6	0.0	1.1187814232737702E-4	0.0
16-17	0.0	7.99129588052693E-6	0.0	1.1187814232737702E-4	0.0
18-19	0.0	7.99129588052693E-6	0.0	1.238650861481674E-4	0.0
20-21	0.0	7.99129588052693E-6	0.0	1.3585202996895782E-4	0.0
22-23	0.0	7.99129588052693E-6	0.0	1.598259176105386E-4	0.0
24-25	0.0	7.99129588052693E-6	0.0	2.197606367144906E-4	0.0
26-27	0.0	7.99129588052693E-6	0.0	3.356344269821311E-4	0.0
28-29	0.0	7.99129588052693E-6	0.0	8.390860674553277E-4	0.0
30-31	0.0	7.99129588052693E-6	0.0	0.0020577586892356844	0.0
32-33	0.0	7.99129588052693E-6	0.0	0.0045909994833627215	0.0
34-35	0.0	7.99129588052693E-6	0.0	0.008514725760701444	0.0
36-37	0.0	7.99129588052693E-6	0.0	0.01466802358870718	0.0
38-39	0.0	7.99129588052693E-6	0.0	0.02359430108725576	0.0
40-41	0.0	7.99129588052693E-6	7.99129588052693E-6	0.03350350797910916	0.0
42-43	0.0	7.99129588052693E-6	7.99129588052693E-6	0.04211812493831718	0.0
44-45	0.0	7.99129588052693E-6	7.99129588052693E-6	0.052678622444433526	0.0
46-47	0.0	7.99129588052693E-6	7.99129588052693E-6	0.064317944894421	0.0
48-49	0.0	7.99129588052693E-6	7.99129588052693E-6	0.07573750670769397	0.0
50-51	0.0	7.99129588052693E-6	7.99129588052693E-6	0.08698525565953563	0.0
52-53	0.0	7.99129588052693E-6	7.99129588052693E-6	0.0976856008435612	0.0
54-55	0.0	7.99129588052693E-6	7.99129588052693E-6	0.10956865781790474	0.0
56-57	0.0	7.99129588052693E-6	7.99129588052693E-6	0.12149566691959118	0.0
58-59	0.0	7.99129588052693E-6	7.99129588052693E-6	0.13268747680026916	0.0
60-61	0.0	7.99129588052693E-6	7.99129588052693E-6	0.1428404182164786	0.0
62-63	0.0	7.99129588052693E-6	7.99129588052693E-6	0.15153095248655163	0.0
64-65	0.0	7.99129588052693E-6	7.99129588052693E-6	0.16114448143082555	0.0
66-67	0.0	7.99129588052693E-6	7.99129588052693E-6	0.17078997555862155	0.0
68-69	7.99129588052693E-6	7.99129588052693E-6	7.99129588052693E-6	0.181610190180855	0.0
70-71	7.99129588052693E-6	7.99129588052693E-6	7.99129588052693E-6	0.1918790053873321	0.0
72-73	7.99129588052693E-6	7.99129588052693E-6	7.99129588052693E-6	0.20058152660122597	0.0
74-75	7.99129588052693E-6	7.99129588052693E-6	7.99129588052693E-6	0.20892843514843631	0.0
76-77	7.99129588052693E-6	7.99129588052693E-6	7.99129588052693E-6	0.21725536545594537	0.0
78-79	7.99129588052693E-6	7.99129588052693E-6	7.99129588052693E-6	0.22458737942632884	0.0
80-81	7.99129588052693E-6	7.99129588052693E-6	7.99129588052693E-6	0.2294261090819879	0.0
82-83	7.99129588052693E-6	7.99129588052693E-6	7.99129588052693E-6	0.23315005296231345	0.0
84-85	7.99129588052693E-6	7.99129588052693E-6	7.99129588052693E-6	0.23665024055798423	0.0
86-87	7.99129588052693E-6	7.99129588052693E-6	7.99129588052693E-6	0.23713371395875613	0.0
88-89	7.99129588052693E-6	7.99129588052693E-6	7.99129588052693E-6	0.23720563562168087	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16595	0.0	39.3931	1
GTATCAA	26980	0.0	28.432383	1
CTTATAC	12675	0.0	24.265562	1
TATACAC	14885	0.0	23.578787	3
ATCAACG	32420	0.0	22.891523	3
CAACGCA	32880	0.0	22.485676	5
AACGCAG	33875	0.0	22.110043	6
TCAACGC	33690	0.0	22.100191	4
TATCAAC	35300	0.0	21.53742	2
TACATGG	36995	0.0	20.365892	2
GTACATG	39080	0.0	19.78343	1
ACATGGG	37310	0.0	19.686539	3
TAACGCC	1365	0.0	19.144178	4
ACGCAGA	39020	0.0	18.95774	7
CGCAGAG	39875	0.0	18.541168	8
GGCGTCG	1780	0.0	18.294521	8
GTACTAG	3925	0.0	18.130527	1
CATGGGG	27010	0.0	17.727327	4
ACACATC	17995	0.0	17.426043	6
ACATCTC	18655	0.0	17.262087	8
>>END_MODULE