FastQCFastQC Report
Fri 27 May 2016
SRR2139927_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139927_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15334074
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159750.044.110311
GTATCAA279100.032.590091
TCAACGC340000.026.0121864
ATCAACG344200.025.6599643
CAACGCA348850.025.3866335
AACGCAG361700.024.3301146
TATCAAC368250.024.2989582
ACGCAGA432150.020.3267927
CGCAGAG437700.020.096438
GTACATG418150.019.4312731
TACATGG419900.018.9412142
ACATGGG428700.018.0583673
GTGGTAT79650.016.8012431
GCAGAGT524700.016.5206389
CATGGGG328100.016.3876614
AGAGTAC464500.016.34871910-11
GAGTACT281700.016.20904212-13
CAGAGTA504200.015.37043810-11
TGGTATC81600.015.0290182
AGTACTT315000.014.63501712-13
Produced by FastQC (version 0.11.5)