##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139927_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15334074
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.839599248053712	31.0	31.0	34.0	30.0	34.0
2	32.01204526598737	31.0	31.0	34.0	30.0	34.0
3	32.04247520913229	33.0	31.0	34.0	30.0	34.0
4	35.76799825017148	37.0	35.0	37.0	35.0	37.0
5	35.58326678220022	37.0	35.0	37.0	33.0	37.0
6	35.591766089038046	37.0	35.0	37.0	33.0	37.0
7	35.484296802010995	37.0	35.0	37.0	33.0	37.0
8	35.451711593409556	37.0	35.0	37.0	33.0	37.0
9	37.03273657085521	39.0	37.0	39.0	33.0	39.0
10-11	36.874867077072935	39.0	37.0	39.0	33.0	39.0
12-13	36.835030436138496	39.0	37.0	39.0	32.5	39.0
14-15	37.976587370062255	40.0	37.0	41.0	33.0	41.0
16-17	37.98928755006661	40.0	37.0	41.0	33.0	41.0
18-19	37.96674523678443	40.0	37.0	41.0	33.0	41.0
20-21	37.92926195464893	40.0	37.0	41.0	33.0	41.0
22-23	37.85165268291995	40.0	37.0	41.0	32.5	41.0
24-25	37.758017801822675	39.5	37.0	41.0	32.0	41.0
26-27	37.59675130202426	39.0	37.0	41.0	32.0	41.0
28-29	37.45904999716923	39.0	36.0	41.0	32.0	41.0
30-31	37.32798077721894	39.0	36.0	40.0	31.5	41.0
32-33	37.49675452140836	39.0	36.0	41.0	32.0	41.0
34-35	37.46980609128575	39.0	36.0	41.0	32.0	41.0
36-37	37.36236763321723	39.0	36.0	40.5	31.5	41.0
38-39	37.20021566836694	39.0	36.0	40.0	31.0	41.0
40-41	37.17076923055495	39.0	36.0	40.0	31.0	41.0
42-43	37.0474329705423	39.0	35.5	40.0	31.0	41.0
44-45	37.09220436992939	39.0	35.5	40.0	31.0	41.0
46-47	37.23836151583582	39.0	36.0	41.0	31.0	41.0
48-49	37.16836585088756	39.0	36.0	41.0	31.0	41.0
50-51	37.04738549465583	39.0	35.5	40.5	31.0	41.0
52-53	36.8948769366701	39.0	35.0	40.0	31.0	41.0
54-55	36.74881332356487	39.0	35.0	40.0	30.0	41.0
56-57	36.56912129177838	38.0	35.0	40.0	30.0	41.0
58-59	36.40445408339444	38.0	35.0	40.0	30.0	41.0
60-61	36.19191049791884	38.0	34.0	40.0	29.5	41.0
62-63	35.92068301130655	37.5	34.0	40.0	29.0	41.0
64-65	35.67661233242025	37.0	34.0	40.0	29.0	41.0
66-67	35.38963743629209	36.5	34.0	39.0	29.0	41.0
68-69	35.05670191004931	36.0	33.5	39.0	28.0	40.5
70-71	34.699972852234716	36.0	33.0	39.0	28.0	40.0
72-73	34.404802343981075	35.0	33.0	38.0	28.0	40.0
74-75	34.04356665015342	35.0	32.5	37.5	28.0	39.5
76-77	32.97320099774406	34.0	31.0	36.0	26.0	39.0
78-79	33.33061473813905	35.0	32.0	36.0	27.0	39.0
80-81	33.21146192284115	35.0	32.0	36.0	27.0	38.5
82-83	32.78324597589229	34.0	31.5	35.5	26.5	37.0
84-85	32.539504511572915	34.0	31.0	35.0	26.0	37.0
86-87	32.453950642476784	34.0	31.0	35.0	26.0	36.0
88-89	32.29006555956656	34.0	31.0	35.0	26.0	36.0
90-91	32.157703607402965	34.0	31.0	35.0	26.0	36.0
92-93	32.07340100483124	34.0	31.0	35.0	26.0	35.0
94-95	32.01982682010594	34.0	31.0	35.0	26.0	35.0
96-97	31.927039789573776	34.0	31.0	35.0	26.0	35.0
98-99	32.13327410796412	34.0	31.0	35.0	26.5	35.0
100-101	31.228762425904165	33.0	29.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	1.0
17	0.0
18	5.0
19	26.0
20	134.0
21	813.0
22	3559.0
23	11616.0
24	30186.0
25	63162.0
26	111934.0
27	178157.0
28	256132.0
29	343239.0
30	440712.0
31	553147.0
32	692683.0
33	879615.0
34	1143246.0
35	1514000.0
36	2111327.0
37	2870415.0
38	2702495.0
39	1382368.0
40	45100.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.5445316097992	21.9468159603247	14.749635354570481	24.75901707530562
2	14.61522253095741	22.60582426284682	40.06450027126191	22.714452934933856
3	17.514185565700647	26.919749240792058	31.229771763836002	24.336293429671294
4	12.767157527834586	18.56855886476592	38.326110528639134	30.33817307876036
5	12.923499651821166	38.0841908027834	35.908245910382334	13.08406363501311
6	30.312577075081286	38.18340774930394	18.31802820307245	13.185986972542327
7	26.03798573034146	32.760980545678855	23.744805196583766	17.456228527395915
8	24.27787292535565	37.099162296986435	21.343884214984225	17.27908056267369
9	25.57187998440597	16.29105872320689	21.342410373133717	36.79465091925343
10-11	24.264018159818455	26.720315814310013	29.222762326567615	19.792903699303917
12-13	24.708293438521295	24.422462680172274	29.305897441214906	21.563346440091525
14-15	22.01576371680481	25.801284120580085	26.62494324730662	25.558008915308484
16-17	21.765850122596554	28.745621813724654	27.44121536478242	22.04731269889638
18-19	21.8366863548765	28.079195997412526	28.90056666019516	21.183550987515815
20-21	22.506118947733057	27.383303199057192	28.63099820981302	21.479579643396736
22-23	22.32017431585849	27.277946044100588	28.465476254499823	21.9364033855411
24-25	22.07805968667596	27.398033388812692	28.57458319279718	21.949323731714166
26-27	22.044673324171008	27.489455426484867	28.536706160756914	21.92916508858721
28-29	22.009072609614574	27.593939488939924	28.49441444406998	21.90257345737552
30-31	22.261402594595253	27.45255913304261	28.361272329325622	21.924765943036515
32-33	22.084566584392466	27.627966267412667	28.296057093798012	21.99141005439686
34-35	22.18957647142323	27.599868284337475	28.348888050764998	21.8616671934743
36-37	22.154174111686608	27.589157301352678	28.29909622003643	21.95757236692429
38-39	22.044863426272762	27.633481308081542	28.36321831975312	21.958436945892576
40-41	22.255415444328555	27.48476198879893	28.217531668469416	22.04229089840311
42-43	22.13157446292242	27.657331066114583	27.988877973693683	22.22221649726932
44-45	22.212552614101067	27.65545091137439	27.906171113657596	22.225825360866942
46-47	22.298895432344693	27.618929211189357	27.69619008634295	22.385985270122998
48-49	22.187294036746955	27.800979264479516	27.647028481773006	22.364698217000527
50-51	22.210496207711223	27.843039336452836	27.574198905293684	22.372265550542256
52-53	22.36886343247533	27.67670232098084	27.451370735859037	22.503063510684793
54-55	22.257978492873377	27.567056143568262	27.54997362718839	22.624991736369967
56-57	22.261561284338484	27.639523544931127	27.580302133466837	22.518613037263552
58-59	22.254391357069082	27.7021699642393	27.524111810174105	22.519326868517513
60-61	22.327906045751604	27.64770991711761	27.52924538454457	22.49513865258622
62-63	22.293381470342798	27.75614931813029	27.483744780975094	22.46672443055182
64-65	22.21666680025328	27.757532281205165	27.46175148080761	22.564049437733942
66-67	22.306068048223633	27.832907223465988	27.23911657201204	22.621908156298346
68-69	22.260578675217758	27.803082907052847	27.290546716468857	22.645791701260542
70-71	22.198571795990404	27.612008941373023	27.406955367885637	22.782463894750933
72-73	22.302159232259598	27.58950875399116	27.282942461991787	22.825389551757453
74-75	22.260236147551137	27.631405049342927	27.250580930575463	22.857777872530473
76-77	22.47102245049257	27.60336770322865	27.162468113567574	22.763141732711215
78-79	22.43108578537469	27.578858486688222	27.129342832303287	22.860712895633796
80-81	22.422137766788687	27.597761981556317	27.10432527784217	22.875774973812824
82-83	22.38325025273135	27.596021082550248	27.158941161599852	22.861787503118546
84-85	22.532868255274995	27.58672068591605	27.171252438785874	22.709158620023082
86-87	22.412011463378395	27.57470414764955	27.272731772249347	22.74055261672271
88-89	22.40868965144396	27.53068266783704	27.336731905050087	22.723895775668908
90-91	22.500117552918578	27.48941191499738	27.414136435454296	22.596334096629747
92-93	22.527576203682294	27.407870586878413	27.4431938717715	22.621359337667794
94-95	22.36819760308388	27.463771979847536	27.528644184759983	22.639386232308606
96-97	22.15975488669366	27.472020771623594	27.682541324908577	22.68568301677417
98-99	22.212658925709434	28.108808074091087	27.820049648829947	21.858483351369536
100-101	25.169299963314256	35.81116259588904	29.020630207560917	9.998907233235787
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2957.0
1	2848.0
2	4158.5
3	5918.5
4	7862.0
5	12022.5
6	15536.5
7	18216.0
8	19922.0
9	19790.5
10	18724.0
11	17638.0
12	19328.5
13	25206.0
14	38600.5
15	57061.0
16	71517.0
17	78725.5
18	80711.5
19	79726.5
20	77543.0
21	76184.0
22	77974.5
23	86231.0
24	101862.0
25	124704.5
26	154489.5
27	187218.5
28	221877.0
29	259354.0
30	294313.0
31	328647.0
32	365043.5
33	399207.0
34	431867.0
35	464240.0
36	500394.5
37	533393.5
38	559514.5
39	581701.0
40	601939.5
41	621686.0
42	636496.5
43	652208.0
44	667650.0
45	678257.5
46	684223.5
47	684436.5
48	681260.5
49	672542.0
50	659739.0
51	644264.0
52	620828.5
53	591780.0
54	560457.5
55	523254.5
56	481527.5
57	436834.5
58	389676.0
59	341256.5
60	290727.5
61	240391.5
62	194197.0
63	153467.5
64	117043.5
65	88713.0
66	67772.0
67	52116.5
68	40031.5
69	28834.0
70	20236.5
71	14496.5
72	10152.5
73	6993.0
74	4790.5
75	3273.5
76	2277.0
77	1526.5
78	1053.0
79	735.0
80	517.0
81	363.0
82	260.5
83	184.5
84	124.0
85	89.0
86	71.5
87	61.0
88	42.5
89	27.0
90	19.0
91	14.0
92	11.5
93	7.0
94	2.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0012064634616997414
3	6.5214241172959E-6
4	1.9564272351887697E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0015749239243269596
18-19	0.0012586348546381086
20-21	1.6304365193780315E-5
22-23	0.00256662562239856
24-25	1.7942488517459803E-4
26-27	0.0014328168654098982
28-29	6.433275487192442E-4
30-31	2.2244591121352783E-4
32-33	0.0010803400634688295
34-35	0.01697160378718269
36-37	0.03249584624746459
38-39	0.007954661653438234
40-41	0.004705399071990738
42-43	0.00743959527777357
44-45	0.005294198543444503
46-47	0.0030560003046814638
48-49	0.0013835986849790613
50-51	1.7077278938348444E-4
52-53	1.1409367517663172E-4
54-55	0.004661699776473172
56-57	0.012178271153891192
58-59	0.009924133258196022
60-61	0.008442981910318188
62-63	0.0019258642503279396
64-65	0.0014467220944239844
66-67	0.0015608773548972954
68-69	0.0
70-71	0.0
72-73	6.535176901004496E-4
74-75	8.799082023729329E-4
76-77	7.582179113817205E-5
78-79	9.048432713694539E-4
80-81	0.002724710520395108
82-83	0.004665154284332619
84-85	0.013048413438810347
86-87	0.008438141567864113
88-89	0.07818248922038913
90-91	0.0
92-93	0.0
94-95	1.439633748315144E-4
96-97	0.009015131070053238
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	476.0
20-21	1551.0
22-23	3173.0
24-25	7316.0
26-27	8149.0
28-29	16576.0
30-31	53952.0
32-33	71470.0
34-35	99859.0
36-37	145653.0
38-39	158019.0
40-41	166807.0
42-43	184684.0
44-45	210360.0
46-47	207564.0
48-49	191940.0
50-51	180480.0
52-53	163505.0
54-55	179402.0
56-57	186430.0
58-59	191925.0
60-61	189157.0
62-63	189135.0
64-65	204484.0
66-67	213462.0
68-69	215963.0
70-71	211931.0
72-73	206713.0
74-75	211413.0
76-77	210657.0
78-79	223026.0
80-81	226705.0
82-83	220515.0
84-85	221613.0
86-87	232736.0
88-89	248855.0
90-91	270190.0
92-93	293815.0
94-95	371547.0
96-97	450535.0
98-99	1230246.0
100-101	7062085.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.11145368968558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.70148448613736	78.10436680913753
2	6.508008065572595	11.208280703013093
3	1.5592223191967565	4.02800701594307
4	0.5610432404430611	1.9324899606929518
5	0.25518289475936856	1.0987085012235638
6	0.13304123903944642	0.6873824696618208
7	0.08053381381040654	0.48544186448720095
8	0.05178751419439961	0.3567598504203981
9	0.0308559224206286	0.2391343501118842
>10	0.11542851411312743	1.6035376867679252
>50	0.002738613324461308	0.15847241630090816
>100	6.480112646754801E-4	0.08104862950169338
>500	2.5365723793262987E-5	0.016369742737975965
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.521424117295899E-6	0.0
2	0.0	0.0	0.0	6.521424117295899E-6	0.0
3	0.0	0.0	0.0	1.3042848234591798E-5	0.0
4	0.0	3.912854470377539E-5	0.0	2.6085696469183597E-5	0.0
5	0.0	4.56499688210713E-5	0.0	2.6085696469183597E-5	0.0
6	0.0	5.217139293836719E-5	0.0	2.6085696469183597E-5	6.521424117295899E-6
7	0.0	5.86928170556631E-5	0.0	3.26071205864795E-5	6.521424117295899E-6
8	0.0	7.17356652902549E-5	0.0	5.217139293836719E-5	6.521424117295899E-6
9	1.3042848234591798E-5	1.8912129940158108E-4	0.0	8.477851352484669E-5	1.9564272351887697E-5
10-11	1.3042848234591798E-5	1.8912129940158108E-4	0.0	8.477851352484669E-5	1.9564272351887697E-5
12-13	1.3042848234591798E-5	1.9238201146022904E-4	0.0	8.803922558349464E-5	1.9564272351887697E-5
14-15	1.3042848234591798E-5	2.673783888091319E-4	0.0	9.782136175943848E-5	2.9346408527831545E-5
16-17	1.3042848234591798E-5	2.836819491023716E-4	0.0	1.1086420999403029E-4	3.26071205864795E-5
18-19	1.3042848234591798E-5	3.1954978174749906E-4	0.0	1.5325346675645364E-4	4.2389256762423345E-5
20-21	1.3042848234591798E-5	3.554176143926265E-4	0.0	2.445534043985962E-4	5.5432104997015145E-5
22-23	1.3042848234591798E-5	4.1084971938964163E-4	0.0	5.347567776182638E-4	6.847495323160694E-5
24-25	1.3042848234591798E-5	4.499782640934171E-4	0.0	0.0016140524690307352	7.825708940755079E-5
26-27	1.3042848234591798E-5	4.695425364453048E-4	0.0	0.005109535795901337	7.825708940755079E-5
28-29	1.3042848234591798E-5	4.728032485039527E-4	0.0	0.029649654749285805	8.151780146619875E-5
30-31	1.3042848234591798E-5	5.021496570317843E-4	0.0	0.047189024912753126	8.477851352484669E-5
32-33	1.3042848234591798E-5	5.151925052663761E-4	0.0	0.06332954960306048	9.12999376421426E-5
34-35	1.9564272351887697E-5	5.83667458497983E-4	0.0	0.08640560884211201	9.782136175943848E-5
36-37	1.9564272351887697E-5	6.586638358468858E-4	0.0	0.1154683354208412	9.782136175943848E-5
38-39	1.9564272351887697E-5	6.749673961401255E-4	0.0	0.1624878033065446	9.782136175943848E-5
40-41	1.9564272351887697E-5	7.173566529025489E-4	0.0	0.23921887947064818	9.782136175943848E-5
42-43	1.9564272351887697E-5	7.564851976063243E-4	0.0	0.2801799443513837	9.782136175943848E-5
44-45	1.9564272351887697E-5	8.575672714244108E-4	0.0	0.33465992142727363	9.782136175943848E-5
46-47	1.9564272351887697E-5	9.293029367146656E-4	0.0	0.3981329423609147	1.0108207381808644E-4
48-49	1.9564272351887697E-5	9.358243608319616E-4	0.0	0.448775061343776	1.0434278587673439E-4
50-51	2.6085696469183597E-5	0.0010206028743568083	0.0	0.5016442466626938	1.1086420999403029E-4
52-53	2.6085696469183597E-5	0.0010303850105327522	0.0	0.5602001138118937	1.173856341113262E-4
54-55	2.6085696469183597E-5	0.001040167146708696	0.0	0.6988945012264842	1.239070582286221E-4
56-57	2.6085696469183597E-5	0.0010532099949432877	0.0	0.8225341810662972	1.30428482345918E-4
58-59	2.6085696469183597E-5	0.0010792956914124714	0.0	0.9246205541984471	1.3694990646321388E-4
60-61	2.6085696469183597E-5	0.001108642099940303	0.0	0.9884848605791259	1.3694990646321388E-4
62-63	2.6085696469183597E-5	0.0011282063722921907	0.0	1.0416083814386183	1.3694990646321388E-4
64-65	2.6085696469183597E-5	0.0012781991269899963	0.0	1.065026815443828	1.3694990646321388E-4
66-67	2.6085696469183597E-5	0.00128798126316594	0.0	1.076305618454691	1.4021061852186184E-4
68-69	2.6085696469183597E-5	0.0013238490958110676	0.0	1.0870040147191151	1.434713305805098E-4
70-71	2.6085696469183597E-5	0.001349934792280251	0.0	1.0969654900582846	1.434713305805098E-4
72-73	2.6085696469183597E-5	0.0013662383525734909	0.0	1.1052150915666639	1.5651417881510157E-4
74-75	2.6085696469183597E-5	0.0013890633369840265	0.0	1.1136733786467965	1.5651417881510157E-4
76-77	2.6085696469183597E-5	0.0014118883213945623	0.0	1.1215316947081382	1.5651417881510157E-4
78-79	2.6085696469183597E-5	0.001421670457570506	0.0	1.1287704754783365	1.5651417881510157E-4
80-81	2.6085696469183597E-5	0.0014281918816878019	0.0	1.13310396180428	1.630356029323975E-4
82-83	2.6085696469183597E-5	0.001437974017863746	0.0	1.1361233811705878	1.6955702704969338E-4
84-85	2.6085696469183597E-5	0.0014412347299223938	0.0	1.1391851897936582	1.6955702704969338E-4
86-87	2.6085696469183597E-5	0.0014607990022742815	0.0	1.1396156037853997	1.6955702704969338E-4
88-89	2.6085696469183597E-5	0.0015064489710953526	0.0	1.1397166858592178	1.6955702704969338E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15975	0.0	44.11031	1
GTATCAA	27910	0.0	32.59009	1
TCAACGC	34000	0.0	26.012186	4
ATCAACG	34420	0.0	25.659964	3
CAACGCA	34885	0.0	25.386633	5
AACGCAG	36170	0.0	24.330114	6
TATCAAC	36825	0.0	24.298958	2
ACGCAGA	43215	0.0	20.326792	7
CGCAGAG	43770	0.0	20.09643	8
GTACATG	41815	0.0	19.431273	1
TACATGG	41990	0.0	18.941214	2
ACATGGG	42870	0.0	18.058367	3
GTGGTAT	7965	0.0	16.801243	1
GCAGAGT	52470	0.0	16.520638	9
CATGGGG	32810	0.0	16.387661	4
AGAGTAC	46450	0.0	16.348719	10-11
GAGTACT	28170	0.0	16.209042	12-13
CAGAGTA	50420	0.0	15.370438	10-11
TGGTATC	8160	0.0	15.029018	2
AGTACTT	31500	0.0	14.635017	12-13
>>END_MODULE