FastQCFastQC Report
Fri 27 May 2016
SRR2139926_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139926_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14191782
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA231250.053.985541
GTATCAA407800.034.973681
TGGTATC87850.028.3269462
GTGGTAT91750.028.1643051
TCAACGC516000.027.7924354
ATCAACG517700.027.7011743
TATCAAC529400.027.1994022
CAACGCA529600.027.0708895
AACGCAG545250.026.3151686
ACGCAGA629500.022.7535677
CGCAGAG638800.022.4353148
GTACATG512650.022.3829561
TACATGG517950.022.177982
ACATGGG521600.021.4966533
GCAGAGT735050.019.4975599
GAGTACT451700.019.3588712-13
CATGGGG375700.019.3509524
CAGAGTA714350.018.79773110-11
AGAGTAC675100.017.6723510-11
GTACTTT502700.017.36227414-15
Produced by FastQC (version 0.11.5)