##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139926_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14191782
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.530888932764046	31.0	18.0	33.0	18.0	33.0
2	28.998937060899046	31.0	27.0	33.0	18.0	33.0
3	31.145700589256514	33.0	30.0	33.0	27.0	33.0
4	31.426992536948497	33.0	31.0	33.0	29.0	33.0
5	32.56097852968711	33.0	33.0	33.0	31.0	34.0
6	36.75504217863549	38.0	37.0	38.0	35.0	38.0
7	36.96614153176817	38.0	38.0	38.0	35.0	38.0
8	37.19667748560399	38.0	38.0	38.0	36.0	38.0
9	37.24509839567716	38.0	38.0	38.0	36.0	38.0
10-11	37.2953070305054	38.0	38.0	38.0	36.5	38.0
12-13	37.31672005672015	38.0	38.0	38.0	37.0	38.0
14-15	37.32908605135	38.0	38.0	38.0	37.0	38.0
16-17	37.34714276896305	38.0	38.0	38.0	37.0	38.0
18-19	37.346422845277644	38.0	38.0	38.0	37.0	38.0
20-21	37.31122097996158	38.0	38.0	38.0	37.0	38.0
22-23	37.325685728177135	38.0	38.0	38.0	37.0	38.0
24-25	37.347510182366086	38.0	38.0	38.0	37.0	38.0
26-27	37.31929647547165	38.0	38.0	38.0	37.0	38.0
28-29	37.29104687485851	38.0	38.0	38.0	37.0	38.0
30-31	37.28970034152232	38.0	38.0	38.0	37.0	38.0
32-33	37.22643976714686	38.0	38.0	38.0	37.0	38.0
34-35	37.22955148039468	38.0	38.0	38.0	37.0	38.0
36-37	37.22161518256364	38.0	38.0	38.0	37.0	38.0
38-39	37.20830811129121	38.0	38.0	38.0	37.0	38.0
40-41	37.198084252435976	38.0	38.0	38.0	37.0	38.0
42-43	37.164085091984575	38.0	38.0	38.0	36.0	38.0
44-45	37.12537087298229	38.0	38.0	38.0	36.0	38.0
46-47	37.07881064557	38.0	38.0	38.0	36.0	38.0
48-49	37.02193120802893	38.0	38.0	38.0	36.0	38.0
50-51	36.987955222111225	38.0	38.0	38.0	36.0	38.0
52-53	36.96058292666808	38.0	38.0	38.0	36.0	38.0
54-55	36.94519225828114	38.0	38.0	38.0	36.0	38.0
56-57	36.92791489979834	38.0	38.0	38.0	36.0	38.0
58-59	36.94757347603252	38.0	38.0	38.0	36.0	38.0
60-61	36.98738900844823	38.0	38.0	38.0	36.0	38.0
62-63	37.01014357588164	38.0	38.0	38.0	36.0	38.0
64-65	37.019113389521664	38.0	38.0	38.0	36.0	38.0
66-67	37.03523609063024	38.0	38.0	38.0	36.0	38.0
68-69	37.04538397185412	38.0	38.0	38.0	36.0	38.0
70-71	37.041294343034636	38.0	38.0	38.0	36.0	38.0
72-73	37.028411756479784	38.0	38.0	38.0	36.0	38.0
74-75	37.03285687494542	38.0	38.0	38.0	36.0	38.0
76-77	37.03881170885595	38.0	38.0	38.0	36.0	38.0
78-79	37.021198405275655	38.0	38.0	38.0	36.0	38.0
80-81	37.005494650364454	38.0	38.0	38.0	36.0	38.0
82-83	37.01048790387827	38.0	38.0	38.0	36.0	38.0
84-85	36.965663026681526	38.0	38.0	38.0	36.0	38.0
86-87	36.959992373590126	38.0	38.0	38.0	36.0	38.0
88-89	36.972909111253976	38.0	38.0	38.0	36.0	38.0
90-91	36.95837014164505	38.0	38.0	38.0	36.0	38.0
92-93	36.966154453186306	38.0	38.0	38.0	36.0	38.0
94-95	36.91864712694611	38.0	38.0	38.0	35.5	38.0
96-97	36.89369520200991	38.0	38.0	38.0	35.0	38.0
98-99	36.969621510361186	38.0	38.0	38.0	35.0	38.0
100	35.469240384505255	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	32.0
19	76.0
20	173.0
21	396.0
22	1015.0
23	2719.0
24	6398.0
25	13046.0
26	23503.0
27	39604.0
28	62359.0
29	94030.0
30	131446.0
31	174455.0
32	224214.0
33	291060.0
34	427190.0
35	791900.0
36	2424810.0
37	9483353.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.791333886047575	20.831985722441342	13.22477332304005	24.151907068471033
2	15.732080027216536	20.678240271624198	40.63106090094742	22.95861880021185
3	18.556859173851457	26.186535277951705	30.126977711467102	25.129627836729735
4	12.768368341621933	17.281860727567548	37.94035167676617	32.009419254044346
5	13.284041001591543	37.4509046205501	35.55745672651621	13.707597651342157
6	30.722431091645653	37.122587013241784	18.179966273358293	13.975015621754276
7	26.671294697170516	32.765483573521635	22.776857761766635	17.786363967541217
8	24.9820987949223	36.33082864435207	20.8234667077045	17.863605853021134
9	26.07358258462538	15.844930537969088	20.948355886526443	37.13313099087909
10-11	24.779551997064218	26.230824994352364	28.567885273322265	20.421737735261154
12-13	25.41753037074555	24.143440901220156	28.79751112298653	21.641517605047767
14-15	22.5696920936356	25.282885546015294	26.61496984663378	25.53245251371533
16-17	21.802526278940867	28.39392896536883	27.41596862184044	22.38757613384986
18-19	21.860404845564847	27.309611294762	29.52154986597173	21.308433993701424
20-21	22.761129007925074	26.317202101900293	29.467875189575988	21.453793700598645
22-23	22.581845046720527	26.242071399281976	29.328520426970268	21.84756312702723
24-25	22.146957762016857	26.440955035667145	29.468435803541293	21.943651398774698
26-27	22.085677577566752	26.461911843212793	29.460822299156458	21.991588280063993
28-29	22.04997225816297	26.600705407077257	29.427107794313812	21.92221454044596
30-31	22.218116471677256	26.541327038989433	29.31300876186624	21.927547727467072
32-33	21.97294056231502	26.647253128423117	29.42916168477424	21.950644624487623
34-35	22.129849765709018	26.659106971066844	29.35802420085786	21.85301906236628
36-37	22.07151234539996	26.633002662475107	29.265762888657022	22.029722103467908
38-39	21.97713886353588	26.648217085874165	29.46586288604383	21.908781164546124
40-41	22.201168568538392	26.565980151528223	29.299091219849515	21.93376006008387
42-43	22.104884267842973	26.685017520900466	29.019429483010363	22.190668728246198
44-45	22.213185446360797	26.771166269951056	28.900420123809578	22.115228159878566
46-47	22.375833800341212	26.862057600993328	28.463502730831625	22.29860586783383
48-49	22.250499957269255	27.07023261345088	28.3584074611436	22.320859968136265
50-51	22.227505510952568	27.149868263129456	28.25353920307561	22.36908702284236
52-53	22.40886280871079	27.151231120707457	28.08200690646584	22.35789916411591
54-55	22.344577386649764	26.98996511460881	28.157435846761548	22.508021651979877
56-57	22.399902498054196	27.07109628454974	28.04976615875215	22.479235058643916
58-59	22.3036665552709	27.13415367106895	28.068481011267636	22.493698762392516
60-61	22.329475944800745	27.12435737580171	28.17683195637299	22.369334723024554
62-63	22.285830880517047	27.24356113860357	28.0515118674437	22.41909611343568
64-65	22.296611459187297	27.24386533800064	27.993288767501635	22.46623443531043
66-67	22.388255553054666	27.238489807109122	27.823490474861114	22.549764164975098
68-69	22.37964482161125	27.26947703941283	27.80670073951827	22.54417739945765
70-71	22.436821498145576	27.028048490874575	27.86448219992233	22.670647811057517
72-73	22.41532566559852	27.0447219631408	27.829529498682525	22.710422872578153
74-75	22.37636184309138	27.16321927886063	27.770499368322433	22.689919509725563
76-77	22.60422783855736	27.11360869881804	27.56516750246176	22.71699596016284
78-79	22.51210058339281	27.146688366811222	27.55730362275588	22.78390742704009
80-81	22.50203842889487	27.237353627025442	27.49076791609923	22.76984002798046
82-83	22.587780927879997	27.189908944811613	27.552234436134253	22.670075691174134
84-85	22.605846113027305	27.2479316428131	27.43591409252197	22.71030815163763
86-87	22.574244660846666	27.299418736043847	27.50781375610105	22.61852284700844
88-89	22.640263520898387	27.304998021899856	27.40973244298769	22.64500601421407
90-91	22.65531883642993	27.26793647972723	27.417768355573358	22.658976328269482
92-93	22.71780951383687	27.24754736305373	27.42146838708229	22.613174736027116
94-95	22.662814597304024	27.315910435281232	27.42309412183113	22.59818084558361
96-97	22.727806893418226	27.340135195391657	27.496375817731927	22.435682093458183
98-99	23.8269681804304	29.334801727061233	27.60221957300459	19.236010519503775
100	30.456792687800444	40.50148633141584	29.041720980783715	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2983.0
1	3238.5
2	5386.0
3	8293.5
4	11091.5
5	15192.5
6	18451.0
7	21624.0
8	24826.0
9	26704.5
10	27461.0
11	28515.5
12	32496.0
13	40740.5
14	55350.5
15	71064.5
16	80960.5
17	84217.5
18	81755.5
19	74532.0
20	64942.5
21	56413.0
22	52720.0
23	56545.5
24	67466.5
25	84468.0
26	106325.5
27	130774.5
28	157213.0
29	186211.5
30	214685.5
31	243560.0
32	273131.5
33	302644.0
34	331588.0
35	361122.5
36	393074.5
37	423391.0
38	451607.0
39	476618.0
40	504316.5
41	532484.5
42	553244.5
43	579892.0
44	608005.5
45	623368.5
46	627438.5
47	629146.5
48	630223.0
49	625293.0
50	619139.0
51	607604.5
52	587176.0
53	561688.0
54	528969.0
55	490851.5
56	450216.5
57	405575.5
58	359809.0
59	310062.0
60	259138.5
61	213319.5
62	171321.5
63	133686.0
64	101189.5
65	75545.5
66	56058.5
67	42547.0
68	32134.5
69	23426.5
70	16932.5
71	12036.0
72	8470.0
73	5568.5
74	3610.0
75	2397.5
76	1549.5
77	999.0
78	646.5
79	412.0
80	273.5
81	191.0
82	144.0
83	107.5
84	71.0
85	42.5
86	25.0
87	17.0
88	9.0
89	3.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0028889958991760163
3	0.0
4	0.0
5	0.003720463011621796
6	2.3957526968776716E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	6.222361733265946E-5
44-45	3.3274779580465666E-5
46-47	0.0
48-49	0.0
50-51	1.5301557939597787E-5
52-53	0.0011815950127114139
54-55	0.002459594437434232
56-57	0.002638310992680262
58-59	0.0018269314775318276
60-61	0.004539444756687773
62-63	0.001806071083540957
64-65	0.0018421297289433571
66-67	2.2466371586130825E-4
68-69	2.1905613094299286E-4
70-71	2.1759969361963136E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	8.05697787325595E-5
80-81	3.626277968348758E-4
82-83	0.0
84-85	4.643376172812346E-6
86-87	0.0
88-89	0.0
90-91	2.891297060919436E-5
92-93	2.3920368799364443E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	149.0
20-21	559.0
22-23	1125.0
24-25	1604.0
26-27	4895.0
28-29	13531.0
30-31	35089.0
32-33	49461.0
34-35	69887.0
36-37	102777.0
38-39	112648.0
40-41	109600.0
42-43	128803.0
44-45	156975.0
46-47	157472.0
48-49	143617.0
50-51	125995.0
52-53	118404.0
54-55	129709.0
56-57	132979.0
58-59	137452.0
60-61	134639.0
62-63	130618.0
64-65	139222.0
66-67	147920.0
68-69	150404.0
70-71	148643.0
72-73	133303.0
74-75	131170.0
76-77	137584.0
78-79	140063.0
80-81	136992.0
82-83	129256.0
84-85	125669.0
86-87	128836.0
88-89	134445.0
90-91	134949.0
92-93	132031.0
94-95	150388.0
96-97	424203.0
98-99	3107842.0
100-101	6460874.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.1934953922289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.75216313212246	73.83602674582578
2	7.564927035713724	12.58705444976395
3	2.012164526794962	5.021970008649694
4	0.7464777432414842	2.484083707710474
5	0.35526580463075175	1.477790204028247
6	0.19479713689613742	0.9723512826472914
7	0.11273236070786052	0.6565019391773137
8	0.06633557282643364	0.44149505378214177
9	0.0427246431908042	0.3198971165785914
>10	0.1482364241577215	1.882639335535354
>50	0.003427543367316474	0.19552748024279995
>100	7.17445310270864E-4	0.0893817158677833
>500	1.531582012504732E-5	0.010015028162470461
>1k	1.531521992388483E-5	0.025265932028085025
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.818532584561967E-5
2	0.0	2.113899438421475E-5	0.0	0.0	2.818532584561967E-5
3	0.0	2.818532584561967E-5	0.0	0.0	2.818532584561967E-5
4	7.046331461404918E-6	9.864864045966884E-5	0.0	0.0	3.523165730702459E-5
5	7.046331461404918E-6	1.0569497192107375E-4	0.0	0.0	3.523165730702459E-5
6	7.046331461404918E-6	1.0569497192107375E-4	0.0	0.0	5.637065169123934E-5
7	7.046331461404918E-6	1.1274130338247868E-4	0.0	0.0	5.637065169123934E-5
8	7.046331461404918E-6	1.197876348438836E-4	0.0	0.0	7.046331461404917E-5
9	7.046331461404918E-6	2.113899438421475E-4	0.0	7.046331461404918E-6	8.4555977536859E-5
10-11	7.046331461404918E-6	2.113899438421475E-4	0.0	1.4092662922809835E-5	8.4555977536859E-5
12-13	1.0569497192107377E-5	2.1843627530355244E-4	0.0	1.4092662922809835E-5	8.4555977536859E-5
14-15	2.113899438421475E-5	2.924227556483041E-4	0.0	2.1138994384214754E-5	1.0921813765177622E-4
16-17	2.113899438421475E-5	2.99469087109709E-4	0.0	3.523165730702459E-5	1.4092662922809835E-4
18-19	2.113899438421475E-5	3.417470758781385E-4	0.0	4.22779887684295E-5	1.69111955073718E-4
20-21	3.523165730702459E-5	4.0868722476148517E-4	0.0	4.932432022983442E-5	1.7615828653512293E-4
22-23	4.932432022983442E-5	4.5801154499131963E-4	0.0	7.750964607545409E-5	2.466216011491721E-4
24-25	4.932432022983442E-5	4.932432022983443E-4	0.0	1.1274130338247867E-4	3.0299225284041145E-4
26-27	4.932432022983442E-5	5.14382196682559E-4	0.0	1.9377411518863523E-4	3.0299225284041145E-4
28-29	5.637065169123934E-5	5.14382196682559E-4	0.0	5.566601854509884E-4	3.0299225284041145E-4
30-31	5.637065169123934E-5	5.707528483737983E-4	0.0	0.0016840732192757752	3.1003858430181635E-4
32-33	6.341698315264426E-5	5.707528483737983E-4	0.0	0.0037451251717367136	3.311775786860311E-4
34-35	6.341698315264426E-5	6.905404832176819E-4	0.0	0.006891312169254009	3.4527024160884096E-4
36-37	6.341698315264426E-5	8.06804952330863E-4	0.0	0.0118942075068515	3.5231657307024586E-4
38-39	6.694014888334671E-5	8.4555977536859E-4	0.0	0.025539428381862125	3.558397388009483E-4
40-41	7.046331461404917E-5	8.807914326756146E-4	0.0	0.04005487119235625	3.628860702623532E-4
42-43	7.046331461404917E-5	9.019304270598293E-4	0.0	0.04781640529709377	3.6993240172375816E-4
44-45	7.046331461404917E-5	9.900095703273908E-4	0.0	0.057420555078988675	3.8050189891586557E-4
46-47	7.046331461404917E-5	0.0010569497192107376	0.0	0.06775400016713898	4.122103904921876E-4
48-49	7.398648034475164E-5	0.001067519216402845	0.0	0.07777036033952607	4.721042079141295E-4
50-51	8.455597753685901E-5	0.0012190153428230507	0.0	0.08768454870572279	4.721042079141295E-4
52-53	9.160230899826393E-5	0.0012472006686686703	0.0	0.1185016793521772	4.967663680290467E-4
54-55	9.864864045966884E-5	0.001268339663052885	0.0	0.15811967799392634	5.249516938746663E-4
56-57	9.864864045966884E-5	0.0012965249888985048	0.0	0.2160933700926353	5.319980253360712E-4
58-59	9.864864045966884E-5	0.0013035713203599097	0.0	0.2561447181192609	5.531370197202859E-4
60-61	9.864864045966884E-5	0.0013176639832827195	0.0	0.2710829408174393	6.024613399501205E-4
62-63	9.864864045966884E-5	0.0013458493091283391	0.0	0.2829806714900215	6.200771686036327E-4
64-65	9.864864045966884E-5	0.0014867759383564375	0.0	0.2881244934568471	6.236003343343352E-4
66-67	9.864864045966884E-5	0.0014938222698178424	0.0	0.2933634408984016	6.271235000650376E-4
68-69	9.864864045966884E-5	0.001536100258586272	0.0	0.29905335355348606	6.341698315264425E-4
70-71	9.864864045966884E-5	0.0015607624187011892	0.0	0.30454949209338195	6.76447820294872E-4
72-73	9.864864045966884E-5	0.0015783782473547015	0.0	0.30859056318649764	7.469111349089212E-4
74-75	9.864864045966884E-5	0.001606563573200321	0.0	0.3118213061615518	7.469111349089212E-4
76-77	9.864864045966884E-5	0.001666457390622263	0.0	0.31482304336411027	7.504343006396237E-4
78-79	9.864864045966884E-5	0.0016840732192757752	0.0	0.31710605475760545	7.539574663703261E-4
80-81	9.864864045966884E-5	0.00169111955073718	0.0	0.3178952438812829	7.68050129293136E-4
82-83	9.864864045966884E-5	0.00169111955073718	0.0	0.31834620909481276	8.244207809843753E-4
84-85	9.864864045966884E-5	0.0017052122136599899	0.0	0.31850122838696365	8.244207809843753E-4
86-87	9.864864045966884E-5	0.0017298743737749072	0.0	0.3186210160218076	8.244207809843753E-4
88	9.864864045966884E-5	0.0017686291968126342	0.0	0.31862806235326896	8.244207809843753E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	23125	0.0	53.98554	1
GTATCAA	40780	0.0	34.97368	1
TGGTATC	8785	0.0	28.326946	2
GTGGTAT	9175	0.0	28.164305	1
TCAACGC	51600	0.0	27.792435	4
ATCAACG	51770	0.0	27.701174	3
TATCAAC	52940	0.0	27.199402	2
CAACGCA	52960	0.0	27.070889	5
AACGCAG	54525	0.0	26.315168	6
ACGCAGA	62950	0.0	22.753567	7
CGCAGAG	63880	0.0	22.435314	8
GTACATG	51265	0.0	22.382956	1
TACATGG	51795	0.0	22.17798	2
ACATGGG	52160	0.0	21.496653	3
GCAGAGT	73505	0.0	19.497559	9
GAGTACT	45170	0.0	19.35887	12-13
CATGGGG	37570	0.0	19.350952	4
CAGAGTA	71435	0.0	18.797731	10-11
AGAGTAC	67510	0.0	17.67235	10-11
GTACTTT	50270	0.0	17.362274	14-15
>>END_MODULE