FastQCFastQC Report
Fri 27 May 2016
SRR2139925_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139925_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11172135
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117300.043.5071681
GTATCAA195850.030.8424821
ACCGAGG129700.026.27897392-93
GAGACCG129850.025.9406488-89
GACCGAG131900.025.84425490-91
AGACCGA128700.025.5197890-91
CGAGACC134050.024.90572588-89
ACGAGAC139200.024.9019286-87
TCAACGC235750.024.8309214
ATCAACG240450.024.480533
CAACGCA243350.024.1221125
TATCAAC256200.023.3239332
AACGCAG250850.023.2391836
CACGAGA142900.023.13724986-87
CCACGAG149050.022.68641984-85
GCCCACG143250.022.62816482-83
CCCACGA143900.022.33098684-85
GTACATG283700.020.6626821
ATCTCCG148550.020.52132674-75
TACATGG285600.020.2411352
Produced by FastQC (version 0.11.5)