##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139925_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11172135
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.795269749246675	31.0	31.0	34.0	30.0	34.0
2	31.974721304388105	31.0	31.0	34.0	30.0	34.0
3	32.0021140990509	31.0	31.0	34.0	30.0	34.0
4	35.736660092274214	37.0	35.0	37.0	35.0	37.0
5	35.55089577775421	37.0	35.0	37.0	33.0	37.0
6	35.55804123383758	37.0	35.0	37.0	33.0	37.0
7	35.445524065006374	37.0	35.0	37.0	33.0	37.0
8	35.41271180486093	37.0	35.0	37.0	33.0	37.0
9	36.99004066814445	39.0	37.0	39.0	33.0	39.0
10-11	36.82824272173582	39.0	37.0	39.0	32.5	39.0
12-13	36.78903002872772	39.0	37.0	39.0	32.0	39.0
14-15	37.92255593939744	40.0	37.0	41.0	33.0	41.0
16-17	37.930371544919566	40.0	37.0	41.0	33.0	41.0
18-19	37.90087534746044	40.0	37.0	41.0	33.0	41.0
20-21	37.86459879794122	40.0	37.0	41.0	32.0	41.0
22-23	37.78493047878989	39.5	37.0	41.0	32.0	41.0
24-25	37.68852178502746	39.0	37.0	41.0	32.0	41.0
26-27	37.52529472393538	39.0	36.0	41.0	32.0	41.0
28-29	37.3813060764862	39.0	36.0	40.5	32.0	41.0
30-31	37.24938571851912	39.0	36.0	40.0	31.0	41.0
32-33	37.423507684549804	39.0	36.0	40.5	32.0	41.0
34-35	37.39551238454615	39.0	36.0	41.0	32.0	41.0
36-37	37.28585159214523	39.0	36.0	40.5	31.0	41.0
38-39	37.12000512730103	39.0	36.0	40.0	31.0	41.0
40-41	37.088973624329356	39.0	36.0	40.0	31.0	41.0
42-43	36.962155736840224	39.0	35.0	40.0	31.0	41.0
44-45	37.007637123262214	39.0	35.5	40.0	31.0	41.0
46-47	37.15494248929498	39.0	36.0	40.5	31.0	41.0
48-49	37.08303595426891	39.0	36.0	40.5	31.0	41.0
50-51	36.956611369704156	39.0	35.0	40.0	31.0	41.0
52-53	36.80511068557492	39.0	35.0	40.0	30.0	41.0
54-55	36.65613407480976	38.5	35.0	40.0	30.0	41.0
56-57	36.47767281357561	38.0	35.0	40.0	30.0	41.0
58-59	36.31155025966143	38.0	34.5	40.0	30.0	41.0
60-61	36.09909044810259	38.0	34.0	40.0	29.0	41.0
62-63	35.82284083945807	37.5	34.0	40.0	29.0	41.0
64-65	35.58416397474396	37.0	34.0	40.0	29.0	41.0
66-67	35.30217087761059	36.5	34.0	39.0	28.5	41.0
68-69	34.96936702089766	36.0	33.0	39.0	28.0	40.0
70-71	34.62058646219336	35.5	33.0	39.0	28.0	40.0
72-73	34.323221686832454	35.0	33.0	38.0	28.0	40.0
74-75	33.96732044131575	35.0	32.0	37.5	27.5	39.5
76-77	32.90312345572486	34.0	31.0	36.0	26.0	39.0
78-79	33.26266299307399	35.0	32.0	36.0	26.5	39.0
80-81	33.13334352967138	34.5	32.0	36.0	27.0	38.0
82-83	32.69709632516822	34.0	31.5	35.5	26.0	37.0
84-85	32.462549357284985	34.0	31.0	35.0	26.0	37.0
86-87	32.381388605782846	34.0	31.0	35.0	26.0	36.0
88-89	32.22060635843786	34.0	31.0	35.0	26.0	36.0
90-91	32.083036750640034	34.0	31.0	35.0	26.0	36.0
92-93	32.003964732714984	34.0	31.0	35.0	26.0	35.0
94-95	31.940418205808122	34.0	31.0	35.0	26.0	35.0
96-97	31.852310365682243	34.0	31.0	35.0	26.0	35.0
98-99	32.068810336144345	34.0	31.0	35.0	26.5	35.0
100-101	31.18607541169758	33.0	29.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	15.0
20	113.0
21	679.0
22	2761.0
23	9161.0
24	23232.0
25	48380.0
26	86701.0
27	137177.0
28	195953.0
29	263742.0
30	336451.0
31	421597.0
32	525620.0
33	662436.0
34	859949.0
35	1132939.0
36	1564520.0
37	2085824.0
38	1897212.0
39	889918.0
40	27750.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.917001987534164	21.761614946471735	14.392190928591535	24.929192137402563
2	15.011915490244728	22.594211320216985	39.11394832014144	23.279924869396844
3	17.743084512151796	26.37040751305055	31.17596254895999	24.710545425837662
4	12.79296556825501	18.348995518491904	38.001529162025655	30.856509751227428
5	12.940928479650488	37.98060979392032	35.96388693835153	13.11457478807766
6	30.825101916509247	38.04581666798691	17.971229312928998	13.157852102574843
7	26.462677008467946	32.531534930431825	23.348491581958147	17.65729647914208
8	24.777860274692348	36.790855105134334	21.058785988533078	17.372498631640237
9	25.581466747403248	15.841403635025891	21.069168963676148	37.50796065389471
10-11	24.42936824519217	26.582202058961872	29.085062971401616	19.903366724444343
12-13	24.792741047257305	24.2212880528207	29.207935636295122	21.778035263626872
14-15	22.00216878868721	25.69915687556586	26.43389558038817	25.86477875535876
16-17	21.905655808142434	28.70462793263376	27.248453060069817	22.14126319915399
18-19	21.94064242472198	27.989687777983253	28.739912491834485	21.32975730546028
20-21	22.57745661656079	27.240616874444573	28.54430864704109	21.637617861953547
22-23	22.361296549598958	27.179518536803528	28.415488968447328	22.043695945150183
24-25	22.149486592260242	27.303161909667516	28.506358667543836	22.04099283052841
26-27	22.168202353667382	27.3653756951355	28.427547445258742	22.03887450593837
28-29	22.084454103003008	27.478002019357433	28.429958558712315	22.007585318927244
30-31	22.322100420939666	27.355050501763582	28.32161163990078	22.001237437395968
32-33	22.17225664277133	27.505799393975654	28.26880611420954	22.053137849043477
34-35	22.2238499535554	27.501334739693206	28.326128813593655	21.94868649315774
36-37	22.242781850417504	27.45080167381655	28.257572041340673	22.04884443442527
38-39	22.11155404992759	27.533796905682784	28.329508365138224	22.025140679251397
40-41	22.31786248260919	27.391461398257665	28.18993203455417	22.100744084578974
42-43	22.186294500403598	27.552754692124786	28.006599457723397	22.254351349748216
44-45	22.273855961866357	27.584153749510993	27.876961734064025	22.265028554558626
46-47	22.376958319600824	27.532378381071364	27.666040400401627	22.42462289892618
48-49	22.27181285659814	27.684889016949594	27.643963722934306	22.399334403517965
50-51	22.27733905707139	27.767622809696007	27.570708054733394	22.38433007849921
52-53	22.400522941266463	27.63885163528423	27.46179270306195	22.49883272038736
54-55	22.335066834299646	27.514189547575285	27.53527341400281	22.61547020412226
56-57	22.309762054947868	27.606487205517922	27.578291470675797	22.50545926885841
58-59	22.31568647670918	27.64919446101614	27.511618187285663	22.523500874989015
60-61	22.256609068886057	27.43000618734714	27.695527674550007	22.6178570692168
62-63	22.489227227803813	27.557397022663434	27.61690983400882	22.336465915523938
64-65	22.152885381939363	27.577022050386823	27.58677287748404	22.683319690189776
66-67	22.269169178529328	27.57823587707808	27.403995162766044	22.748599781626556
68-69	22.14627661071218	27.882116094051028	27.448296972905105	22.523310322331685
70-71	22.137826757675942	27.704807202399785	27.511157435459104	22.646208604465173
72-73	22.21897411288566	27.639329812438618	27.134926715500168	23.00676935917555
74-75	22.13179960581028	27.719594177879927	27.13566401174385	23.01294220456595
76-77	22.35732324515313	27.345742932417338	27.379735953273244	22.91719786915629
78-79	22.286945909429807	27.331075568536527	27.30172639105956	23.080252130974106
80-81	22.631596872670972	27.33463040391956	27.014380178323172	23.019392545086294
82-83	22.52947069286796	27.66653929962152	27.062153239462443	22.741836768048078
84-85	22.327520594834755	27.33028471704595	27.077210848954476	23.264983839164824
86-87	22.236955383940245	27.648558903468313	27.174150516273514	22.94033519631793
88-89	22.576639841308307	27.273591401492492	27.157368815233674	22.992399941965523
90-91	22.69531080455508	27.580184339810483	27.200733284526198	22.523771571108238
92-93	22.312172409706086	27.455143277211768	27.25980831581713	22.972875997265017
94-95	22.455590649008098	27.159581076610838	27.436789025514386	22.94803924886668
96-97	22.324047090831662	27.566358100135645	27.540226263537406	22.569368545495283
98-99	22.470545892620226	27.95022125101363	27.800643215693952	21.77858964067219
100-101	25.069522739947224	35.82059492238158	29.09101808658196	10.018864251089232
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2225.0
1	2088.5
2	2860.0
3	4004.0
4	5277.0
5	8046.0
6	10479.5
7	12543.0
8	14076.5
9	14288.5
10	13737.0
11	12942.0
12	13922.0
13	17830.0
14	27682.5
15	41297.5
16	51611.5
17	56419.5
18	57542.0
19	57034.5
20	55520.0
21	54284.0
22	55395.0
23	61234.5
24	72297.5
25	88504.0
26	109360.0
27	131695.5
28	154859.5
29	179344.0
30	202098.0
31	224870.0
32	249128.5
33	272104.5
34	294479.5
35	316060.5
36	339584.5
37	362200.0
38	381405.5
39	397823.5
40	412786.5
41	429044.5
42	441595.5
43	453623.0
44	466072.5
45	476792.0
46	484264.5
47	487376.0
48	488643.0
49	485940.0
50	479715.5
51	469789.5
52	453299.0
53	433630.0
54	413447.0
55	388303.0
56	359713.5
57	328912.0
58	294243.5
59	256563.5
60	218270.0
61	181078.0
62	146448.5
63	115499.5
64	87830.0
65	66199.5
66	50215.0
67	38683.0
68	29909.5
69	21994.0
70	15746.5
71	11464.0
72	8239.5
73	5754.5
74	4039.0
75	2813.0
76	1935.0
77	1298.5
78	881.5
79	615.5
80	431.5
81	304.5
82	231.5
83	174.5
84	147.0
85	119.0
86	94.0
87	74.5
88	50.0
89	33.5
90	26.5
91	20.5
92	16.5
93	9.0
94	3.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001100953398790831
3	1.7901681281151722E-5
4	2.685252192172758E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.00142765908217185
18-19	0.0013336752554458033
20-21	1.3426969437666436E-5
22-23	0.002627540926076128
24-25	1.2536921794368422E-4
26-27	0.001142261247241775
28-29	4.033678888145995E-4
30-31	1.2118523468328599E-4
32-33	9.678505264251554E-4
34-35	0.016586543579956568
36-37	0.03255272007635787
38-39	0.007721683901514074
40-41	0.004619130048412926
42-43	0.007123025446167105
44-45	0.0049170070669684075
46-47	0.0028885337807319274
48-49	0.001261612781729052
50-51	1.9154617348209236E-4
52-53	1.0430998423876138E-4
54-55	0.004514897605236035
56-57	0.011470204085741606
58-59	0.009631317294322962
60-61	0.00796661996620886
62-63	0.0018434463218452918
64-65	0.0012983124502714274
66-67	0.0013296084037329326
68-69	0.0
70-71	1.1223794894127347E-5
72-73	5.022432007685234E-4
74-75	6.733279308441135E-4
76-77	7.089258969212293E-5
78-79	6.738593126153683E-4
80-81	0.0023686676569661135
82-83	0.004552194035423584
84-85	0.012328898996740636
86-87	0.007938576681693212
88-89	0.07447150562134047
90-91	6.845960079152991E-6
92-93	0.0
94-95	9.46175841103014E-5
96-97	0.008491935626110338
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	378.0
20-21	1143.0
22-23	2303.0
24-25	4811.0
26-27	5753.0
28-29	11037.0
30-31	30184.0
32-33	42578.0
34-35	61534.0
36-37	89418.0
38-39	95592.0
40-41	102300.0
42-43	116494.0
44-45	135880.0
46-47	136125.0
48-49	126438.0
50-51	117433.0
52-53	107041.0
54-55	119943.0
56-57	125107.0
58-59	78823.0
60-61	135877.0
62-63	133091.0
64-65	141829.0
66-67	150959.0
68-69	152663.0
70-71	150423.0
72-73	146054.0
74-75	150150.0
76-77	150581.0
78-79	161113.0
80-81	163895.0
82-83	158675.0
84-85	159677.0
86-87	171074.0
88-89	183805.0
90-91	200029.0
92-93	218614.0
94-95	280141.0
96-97	340605.0
98-99	980718.0
100-101	5331847.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.39728423277482
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.87696717066358	78.51523162856365
2	6.350825831635526	10.97388208977326
3	1.5414815257657588	3.9953945256346697
4	0.5539803139908351	1.914495785889121
5	0.2644046587870211	1.1421922228846053
6	0.13561320551723285	0.7029967597674045
7	0.08102130198750326	0.49000143197066615
8	0.051884573694261714	0.3586149008607585
9	0.032634726163596875	0.25375965409935464
>10	0.10853397210793417	1.4595661925226342
>50	0.0022043114860676835	0.12744974297376344
>100	4.319040981105972E-4	0.057517388909851835
>500	1.6504102555070315E-5	0.008897676150213396
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.6852521921727584E-5	0.0	1.7901681281151722E-5	0.0
3	0.0	2.6852521921727584E-5	0.0	1.7901681281151722E-5	0.0
4	0.0	1.163609283274862E-4	0.0	2.6852521921727584E-5	0.0
5	8.950840640575861E-6	1.163609283274862E-4	0.0	3.5803362562303445E-5	0.0
6	8.950840640575861E-6	1.163609283274862E-4	0.0	3.5803362562303445E-5	1.7901681281151722E-5
7	8.950840640575861E-6	1.163609283274862E-4	0.0	3.5803362562303445E-5	2.6852521921727584E-5
8	8.950840640575861E-6	1.163609283274862E-4	0.0	3.5803362562303445E-5	2.6852521921727584E-5
9	8.950840640575861E-6	2.327218566549724E-4	0.0	7.160672512460689E-5	3.5803362562303445E-5
10-11	4.4754203202879306E-5	2.327218566549724E-4	0.0	1.1188550800719828E-4	3.5803362562303445E-5
12-13	4.4754203202879306E-5	2.3719727697526033E-4	0.0	1.4321345024921378E-4	3.5803362562303445E-5
14-15	5.370504384345517E-5	2.9985316145929135E-4	0.0	1.4321345024921378E-4	4.9229623523167233E-5
16-17	6.265588448403104E-5	3.043285817795793E-4	0.0	1.7006597217094136E-4	6.265588448403104E-5
18-19	6.265588448403104E-5	3.3118110370130687E-4	0.0	2.4167269729554825E-4	6.713130480431897E-5
20-21	6.265588448403104E-5	3.7145988658389824E-4	0.0	4.2964035074764134E-4	7.160672512460689E-5
22-23	6.265588448403104E-5	4.1621408978677755E-4	0.0	6.936901496446292E-4	8.503298608547068E-5
24-25	6.265588448403104E-5	4.52017452349081E-4	0.0	0.0016961843013891258	1.0741008768691033E-4
26-27	6.265588448403104E-5	4.7439455395052065E-4	0.0	0.00426060014491411	1.0741008768691033E-4
28-29	6.265588448403104E-5	4.788699742708086E-4	0.0	0.023701826016244883	1.0741008768691033E-4
30-31	7.160672512460689E-5	5.012470758722483E-4	0.0	0.035803362562303445	1.0741008768691033E-4
32-33	8.055756576518276E-5	5.012470758722483E-4	0.0	0.04374275821049424	1.2978718928835E-4
34-35	8.055756576518276E-5	5.639029603562793E-4	0.0	0.054734390517121395	1.5663971121007757E-4
36-37	8.055756576518276E-5	6.355096854808862E-4	0.0	0.0711905110348201	1.6111513153036552E-4
38-39	8.950840640575861E-5	6.534113667620378E-4	0.0	0.10140407361708392	1.745413924912293E-4
40-41	8.950840640575861E-5	6.892147293243413E-4	0.0	0.16002312897221524	1.879676534520931E-4
42-43	8.950840640575861E-5	7.160672512460689E-4	0.0	0.1853361063037638	1.9244307377238104E-4
44-45	9.398382672604655E-5	8.771823827764345E-4	0.0	0.2247063788613367	2.1034475505353275E-4
46-47	9.845924704633448E-5	0.0010114449923850724	0.0	0.2714879474693065	2.461481176158362E-4
48-49	1.163609283274862E-4	0.0010114449923850724	0.0	0.3109835317958474	2.5062353793612414E-4
50-51	1.2531176896806207E-4	0.0011725601239154378	0.0	0.3513294459832431	2.5062353793612414E-4
52-53	1.2978718928835E-4	0.0011949372255168774	0.0	0.4018837939212156	2.685252192172759E-4
54-55	1.3873802992892583E-4	0.0011994126458371654	0.0	0.5292363545553289	2.8642690049842756E-4
56-57	1.4321345024921378E-4	0.0011994126458371654	0.0	0.6477096812739911	2.8642690049842756E-4
58-59	1.5216429088978965E-4	0.0012038880661574534	0.0	0.753871126691541	2.8642690049842756E-4
60-61	1.5216429088978965E-4	0.001226265167758893	0.0	1.277638517615478	2.953777411390034E-4
62-63	1.5216429088978965E-4	0.0012352160083994688	0.0	1.2918703542339938	3.1327942242015514E-4
64-65	1.5216429088978965E-4	0.001351576936726955	0.0	1.3063707160717266	3.1327942242015514E-4
66-67	1.5216429088978965E-4	0.0013605277773675309	0.0	1.3172325611890654	3.2223026306073103E-4
68-69	1.5216429088978965E-4	0.0013739540383283949	0.0	1.327915389493593	3.3118110370130687E-4
70-71	1.5216429088978965E-4	0.0013784294586486826	0.0	1.3387727591906113	3.3118110370130687E-4
72-73	1.5216429088978965E-4	0.0013829048789689704	0.0	1.3473610907852438	3.5803362562303445E-4
74-75	1.5216429088978965E-4	0.0014321345024921378	0.0	1.3554839786665664	3.5803362562303445E-4
76-77	1.5216429088978965E-4	0.0015305937495384723	0.0	1.3625506673523011	3.625090459433224E-4
78-79	1.5216429088978965E-4	0.0015574462714602	0.0	1.3691340106434446	3.669844662636103E-4
80-81	1.5216429088978965E-4	0.0015574462714602	0.0	1.3731529380910632	3.7145988658389824E-4
82-83	1.5216429088978965E-4	0.0015574462714602	0.0	1.3760798629805315	3.8936156786505E-4
84-85	1.5216429088978965E-4	0.0015798233730616395	0.0	1.3789754599277577	3.938369881853379E-4
86-87	1.5216429088978965E-4	0.001611151315303655	0.0	1.379382723176904	3.938369881853379E-4
88-89	1.5663971121007757E-4	0.0016290529965848068	0.0	1.379521461206833	3.938369881853379E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11730	0.0	43.507168	1
GTATCAA	19585	0.0	30.842482	1
ACCGAGG	12970	0.0	26.278973	92-93
GAGACCG	12985	0.0	25.94064	88-89
GACCGAG	13190	0.0	25.844254	90-91
AGACCGA	12870	0.0	25.51978	90-91
CGAGACC	13405	0.0	24.905725	88-89
ACGAGAC	13920	0.0	24.90192	86-87
TCAACGC	23575	0.0	24.830921	4
ATCAACG	24045	0.0	24.48053	3
CAACGCA	24335	0.0	24.122112	5
TATCAAC	25620	0.0	23.323933	2
AACGCAG	25085	0.0	23.239183	6
CACGAGA	14290	0.0	23.137249	86-87
CCACGAG	14905	0.0	22.686419	84-85
GCCCACG	14325	0.0	22.628164	82-83
CCCACGA	14390	0.0	22.330986	84-85
GTACATG	28370	0.0	20.662682	1
ATCTCCG	14855	0.0	20.521326	74-75
TACATGG	28560	0.0	20.241135	2
>>END_MODULE