FastQCFastQC Report
Fri 27 May 2016
SRR2139924_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139924_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10450063
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG168650.045.38839394-95
GGTATCA118550.044.120851
CCGTCTT70950.033.63286692-93
TATGCCG68100.032.459788-89
GTATCAA198100.031.9215411
TGCCGTC73600.031.06565590-91
GCCGTCT71950.030.47148590-91
ATCTCGT134950.029.3983994-95
ATGCCGT72400.029.19663488-89
CGCTATC238850.028.43936792-93
CGTATGC81600.027.44057786-87
TCGTATG82200.025.53573684-85
TCAACGC240300.025.4400834
CTCGTAT87300.025.22873184-85
ATCAACG243900.025.1322353
CAACGCA246950.024.7049085
TATCAAC257450.024.1781182
AACGCAG253600.023.9757586
GCTATCT266750.023.62189192-93
TCTCGTA89500.023.0058782-83
Produced by FastQC (version 0.11.5)