##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139924_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10450063
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.843696923166874	31.0	31.0	34.0	30.0	34.0
2	32.022999669954146	31.0	31.0	34.0	30.0	34.0
3	32.0517133724457	33.0	31.0	34.0	30.0	34.0
4	35.7726885474279	37.0	35.0	37.0	35.0	37.0
5	35.59137538213884	37.0	35.0	37.0	33.0	37.0
6	35.59767314321454	37.0	35.0	37.0	33.0	37.0
7	35.48628826448223	37.0	35.0	37.0	33.0	37.0
8	35.45530280535151	37.0	35.0	37.0	33.0	37.0
9	37.038181779382576	39.0	37.0	39.0	33.0	39.0
10-11	36.87983670529068	39.0	37.0	39.0	33.0	39.0
12-13	36.83833552008251	39.0	37.0	39.0	32.5	39.0
14-15	37.98243072792958	40.0	37.0	41.0	33.0	41.0
16-17	37.99278688559102	40.0	37.0	41.0	33.0	41.0
18-19	37.96488265190363	40.0	37.0	41.0	33.0	41.0
20-21	37.9242162569315	40.0	37.0	41.0	33.0	41.0
22-23	37.84377198804148	40.0	37.0	41.0	32.5	41.0
24-25	37.747487211945284	39.5	37.0	41.0	32.0	41.0
26-27	37.5868979959636	39.0	36.5	41.0	32.0	41.0
28-29	37.443409741929784	39.0	36.0	41.0	32.0	41.0
30-31	37.3062233760854	39.0	36.0	40.0	31.5	41.0
32-33	37.48818833499499	39.0	36.0	41.0	32.0	41.0
34-35	37.461194196170176	39.0	36.0	41.0	32.0	41.0
36-37	37.35563762521669	39.0	36.0	41.0	31.5	41.0
38-39	37.193207035269836	39.0	36.0	40.0	31.0	41.0
40-41	37.16519449683159	39.0	36.0	40.0	31.0	41.0
42-43	37.03634523103057	39.0	35.5	40.0	31.0	41.0
44-45	37.07599555319756	39.0	35.5	40.0	31.0	41.0
46-47	37.21008948510523	39.0	36.0	41.0	31.0	41.0
48-49	37.13177851857404	39.0	36.0	41.0	31.0	41.0
50-51	37.00315521837567	39.0	35.0	40.5	31.0	41.0
52-53	36.847666795493154	39.0	35.0	40.0	31.0	41.0
54-55	36.70824837031978	39.0	35.0	40.0	30.0	41.0
56-57	36.53541635324298	38.0	35.0	40.0	30.0	41.0
58-59	36.378011866547595	38.0	35.0	40.0	30.0	41.0
60-61	36.170002681692395	38.0	34.0	40.0	29.5	41.0
62-63	35.901355122304864	37.5	34.0	40.0	29.0	41.0
64-65	35.68187307643769	37.0	34.0	40.0	29.0	41.0
66-67	35.39957843967217	36.5	34.0	39.0	29.0	41.0
68-69	35.06583647585105	36.0	33.5	39.0	28.5	40.5
70-71	34.70453905064295	36.0	33.0	39.0	28.0	40.0
72-73	34.41335190176147	35.0	33.0	38.0	28.0	40.0
74-75	34.03620802347773	35.0	32.5	37.0	28.0	39.5
76-77	32.949283809471865	34.0	31.0	36.0	26.0	39.0
78-79	33.258554375838344	35.0	32.0	36.0	27.0	39.0
80-81	33.10925308224836	35.0	32.0	36.0	27.0	37.5
82-83	32.68585828693377	34.0	31.5	35.0	26.0	37.0
84-85	32.48282122108115	34.0	31.0	35.0	26.0	37.0
86-87	32.408757874199715	34.0	31.0	35.0	26.0	36.0
88-89	32.24854187100989	34.0	31.0	35.0	26.0	36.0
90-91	32.141602648880564	34.0	31.0	35.0	26.0	35.5
92-93	32.07676762422318	34.0	31.0	35.0	26.0	35.0
94-95	32.019383712933404	34.0	31.0	35.0	26.0	35.0
96-97	31.90258239577191	34.0	31.0	35.0	26.0	35.0
98-99	32.105715239985294	34.0	31.0	35.0	26.5	35.0
100-101	31.17202231961396	33.0	29.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	4.0
19	21.0
20	98.0
21	536.0
22	2640.0
23	8101.0
24	21499.0
25	44598.0
26	79928.0
27	126123.0
28	180553.0
29	242532.0
30	312291.0
31	388157.0
32	485221.0
33	613056.0
34	794940.0
35	1051673.0
36	1472054.0
37	1991754.0
38	1824378.0
39	781090.0
40	28814.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.433331645943184	21.270943534024628	14.206584209109552	25.089140610922634
2	15.125197453419078	22.28439167983182	39.00104431291569	23.589366553833408
3	17.854234124419776	26.163220705261093	30.93516505504897	25.047380115270162
4	12.895338525710324	18.083096723914487	37.62504589685249	31.396518853522704
5	13.10928938897306	37.812336633760005	35.7566265389979	13.321747438269032
6	31.21474961442816	37.57106536104136	17.83505037242359	13.379134652106883
7	26.74671913461192	32.39601522019532	23.09042538786608	17.766840257326677
8	25.040423201276397	36.51087079570716	20.90647683176647	17.542229171249975
9	25.765270506024702	15.785340241489454	20.880438711230738	37.568950541255106
10-11	24.66578909619971	26.395410247765973	28.839022310200424	20.099778345833897
12-13	25.016447269265267	24.026118311439845	29.01373417557387	21.94370024372102
14-15	22.255899318501715	25.447430316927278	26.225722275549916	26.07094808902109
16-17	22.09590805742956	28.471869315294395	27.08613548943391	22.346087137842133
18-19	22.193848781619103	27.728114687265116	28.556289006602782	21.521747524513003
20-21	22.780644229313975	26.999884061710176	28.371558916388974	21.84791279258688
22-23	22.61466218019226	26.91686520553798	28.259448031947944	22.209024582321817
24-25	22.39537591202633	27.034868066162154	28.335113972984665	22.234642048826846
26-27	22.39024964694063	27.125528990425718	28.27324910736046	22.21097225527319
28-29	22.302533915156538	27.23138349343949	28.286779379140164	22.179303212263807
30-31	22.492363423998103	27.118097419459946	28.156062317243435	22.233476839298515
32-33	22.349340562145606	27.306049233110034	28.07627029794012	22.26833990680424
34-35	22.440846377569613	27.266059672206744	28.1254327983591	22.167661151864543
36-37	22.4051301321512	27.240649076916107	28.100819940795006	22.253400850137687
38-39	22.298657456995485	27.27591843832407	28.197016476184356	22.22840762849609
40-41	22.41292637719191	27.034897622049296	28.14198148067643	22.41019452008236
42-43	22.445311326828605	27.23849430225314	27.88734190967656	22.428852461241693
44-45	22.363571270421033	27.243750603881377	27.783177583680924	22.609500542016665
46-47	22.478106323428342	27.20381819459471	27.590706977264656	22.72736850471229
48-49	22.37519870475015	27.487237464440877	27.5633003425342	22.574263488274777
50-51	22.319888970535054	27.553499493176485	27.512990300645402	22.61362123564306
52-53	22.403841627540054	27.36011873121354	27.2144392134586	23.0216004277878
54-55	22.319723047251852	27.013407346811412	27.461318764667965	23.205550841268774
56-57	22.29629513981046	26.674778824739683	27.883429839125856	23.145496196323997
58-59	22.114640553918097	26.41703346992002	28.022993473178566	23.445332502983316
60-61	22.13395741596588	26.150767342242748	28.464549637695345	23.25072560409603
62-63	21.841330178741806	26.865276266403054	28.380866539267334	22.912527015587806
64-65	21.34036671736886	27.232744189485864	28.130005731918956	23.296883361226325
66-67	21.423160381239224	27.980622366416036	27.437808965453105	23.15840828689164
68-69	21.559076355381745	28.411751391599154	26.820271019237662	23.20890123378144
70-71	21.506028671606835	27.90218435089721	26.82519863699297	23.766588340502988
72-73	21.428721750146227	27.348321542047422	26.71133932744112	24.51161738036523
74-75	22.00765977108697	26.85918294818438	26.828319533085914	24.304837747642733
76-77	22.654296184339927	26.61978292567469	26.668136880028335	24.057784009957047
78-79	22.923103239477385	26.43970471663792	25.733383493409672	24.903808550475024
80-81	22.736606046402684	27.048795412897732	25.627618884014275	24.586979656685312
82-83	22.540442470589213	27.254325060607044	25.678454881967717	24.52677758683603
84-85	22.605276164850803	27.28693606429448	25.691767818755274	24.41601995209944
86-87	23.117921980618515	27.700280863328285	25.614292442268944	23.567504713784256
88-89	23.322465064388876	27.204081300322542	26.09146537076536	23.38198826452322
90-91	22.850810983518528	27.191949773704387	26.568913717715663	23.388325525061422
92-93	22.527814606086906	26.481828379010526	26.422499618616325	24.567857396286243
94-95	22.299393455363056	26.407114564299167	27.442471146718212	23.85102083361956
96-97	21.640746804807623	26.835908652133433	27.812173515897804	23.71117102716114
98-99	21.77663876152564	26.811156892997534	28.359308011892914	23.052896333583913
100-101	24.998703064590114	34.858028803797794	29.973690413781558	10.169577717830542
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2379.0
1	2169.0
2	2935.5
3	4188.0
4	5598.5
5	8546.5
6	11049.5
7	13182.0
8	14687.0
9	14703.0
10	13830.5
11	12836.0
12	13898.5
13	18036.0
14	27951.0
15	41414.0
16	51156.5
17	55055.5
18	55514.0
19	53839.5
20	50470.0
21	47698.5
22	47285.0
23	50700.5
24	59118.5
25	71840.0
26	88144.0
27	105745.0
28	125057.5
29	146299.0
30	165967.0
31	185648.5
32	205792.0
33	225380.0
34	245028.0
35	264047.0
36	285613.5
37	307031.5
38	326226.0
39	343960.5
40	361802.5
41	380055.0
42	395148.5
43	410624.5
44	426671.5
45	440160.0
46	450908.0
47	459025.5
48	464047.5
49	464894.0
50	462759.5
51	456374.0
52	444094.0
53	428659.0
54	409069.5
55	383750.5
56	352834.5
57	318591.5
58	284237.0
59	247709.5
60	209736.0
61	173220.0
62	139720.0
63	110192.5
64	83883.5
65	63565.5
66	48642.5
67	37249.0
68	28723.0
69	21141.5
70	15132.5
71	11100.5
72	7909.0
73	5559.5
74	3969.5
75	2753.0
76	1934.0
77	1328.5
78	901.5
79	617.5
80	435.0
81	323.0
82	246.0
83	180.5
84	131.0
85	99.0
86	79.0
87	61.0
88	44.0
89	26.5
90	13.5
91	7.5
92	8.0
93	5.5
94	2.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0012440116389728943
3	3.8277281199165976E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.001421044064519037
18-19	0.0014306133848188283
20-21	2.3924527875759353E-5
22-23	0.0023305361635326217
24-25	1.531799316099474E-4
26-27	0.0012499215805904897
28-29	5.366577749181586E-4
30-31	1.5835777801579432E-4
32-33	9.24227853358543E-4
34-35	0.01607623685127005
36-37	0.034293813156677615
38-39	0.007717115674016663
40-41	0.004628628626255133
42-43	0.007560126207698973
44-45	0.005396149289694622
46-47	0.0031043015134927417
48-49	0.0013468693007355045
50-51	1.6771794370253713E-4
52-53	1.375181444613298E-4
54-55	0.004594540297695475
56-57	0.011777067023936225
58-59	0.009289493662474387
60-61	0.007813258313218606
62-63	0.0018169613723775008
64-65	0.001353458416365623
66-67	0.0012966544080660846
68-69	0.0
70-71	0.0
72-73	5.996031332673222E-4
74-75	8.011748332091513E-4
76-77	7.302378914234837E-5
78-79	8.428805044590239E-4
80-81	0.0025027653661261326
82-83	0.0046230540939884305
84-85	0.012422479519020533
86-87	0.008001967195022509
88-89	0.07572252914483181
90-91	0.0
92-93	0.0
94-95	1.717365409139535E-4
96-97	0.009765822735520648
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	342.0
20-21	1030.0
22-23	2168.0
24-25	4520.0
26-27	5466.0
28-29	10524.0
30-31	29614.0
32-33	41653.0
34-35	59522.0
36-37	85542.0
38-39	64664.0
40-41	99397.0
42-43	109063.0
44-45	127453.0
46-47	126781.0
48-49	114299.0
50-51	110136.0
52-53	18209.0
54-55	18385.0
56-57	18701.0
58-59	20415.0
60-61	131425.0
62-63	129548.0
64-65	138137.0
66-67	147419.0
68-69	148120.0
70-71	146703.0
72-73	141825.0
74-75	146508.0
76-77	146255.0
78-79	155248.0
80-81	157813.0
82-83	152133.0
84-85	152822.0
86-87	162833.0
88-89	174789.0
90-91	189026.0
92-93	205510.0
94-95	263529.0
96-97	319926.0
98-99	993803.0
100-101	5178807.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.73457631423945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.75115942450941	76.05026467547565
2	7.012855967752615	11.88462758360607
3	1.7642816779018664	4.484869814279705
4	0.6621831593523902	2.244392378005972
5	0.30931720049585004	1.3104930965361257
6	0.16363994178783095	0.8319576681287199
7	0.09552958048391796	0.5666260969447279
8	0.06070385565309064	0.41149723915243086
9	0.04675000227369731	0.35652074718163257
>10	0.13140471912865692	1.710100451272382
>50	0.0019037642382759323	0.10573264211345028
>100	2.707064224612995E-4	0.04291760730304287
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.569320299791494E-6
2	0.0	1.9138640599582988E-5	0.0	9.569320299791494E-6	9.569320299791494E-6
3	0.0	1.9138640599582988E-5	0.0	9.569320299791494E-6	9.569320299791494E-6
4	0.0	7.655456239833195E-5	0.0	9.569320299791494E-6	9.569320299791494E-6
5	0.0	7.655456239833195E-5	0.0	1.9138640599582988E-5	9.569320299791494E-6
6	0.0	7.655456239833195E-5	0.0	1.9138640599582988E-5	9.569320299791494E-6
7	0.0	9.569320299791494E-5	0.0	2.8707960899374482E-5	9.569320299791494E-6
8	0.0	1.0526252329770643E-4	0.0	2.8707960899374482E-5	9.569320299791494E-6
9	0.0	2.4880232779457887E-4	0.0	3.8277281199165976E-5	1.9138640599582988E-5
10-11	0.0	2.4880232779457887E-4	0.0	4.784660149895747E-5	1.9138640599582988E-5
12-13	0.0	2.631563082442661E-4	0.0	6.22005819486447E-5	1.9138640599582988E-5
14-15	0.0	3.2057223004301505E-4	0.0	7.176990224843621E-5	3.349262104927023E-5
16-17	0.0	3.3971087064259807E-4	0.0	8.612388269812345E-5	4.784660149895747E-5
18-19	0.0	3.9234213229145124E-4	0.0	1.2918582404718517E-4	5.7415921798748963E-5
20-21	0.0	4.4018873379040875E-4	0.0	3.492801909423895E-4	5.7415921798748963E-5
22-23	0.0	5.645898976876982E-4	0.0	8.660234871311302E-4	1.0047786314781069E-4
24-25	0.0	6.554984405357173E-4	0.0	0.002133958426853503	1.2440116389728943E-4
26-27	0.0	6.985603818847791E-4	0.0	0.00546886655133084	1.2440116389728943E-4
28-29	0.0	7.081297021845705E-4	0.0	0.028540497794128133	1.2440116389728943E-4
30-31	0.0	7.990382450325898E-4	0.0	0.047650430432811745	1.2440116389728943E-4
32-33	0.0	8.038229051824855E-4	0.0	0.0690904925644946	1.4353980449687242E-4
34-35	0.0	9.521473698292538E-4	0.0	0.0987697394742979	1.531091247966639E-4
36-37	0.0	0.0011100411547758132	0.0	0.1412096750038732	1.531091247966639E-4
38-39	0.0	0.0011387491156751878	0.0	0.22199866163486287	1.5789378494655967E-4
40-41	0.0	0.0012057343577737282	0.0	0.6065083052609348	1.6746310524635115E-4
42-43	0.0	0.0012487962991227898	0.0	0.6746227271548506	1.722477653962469E-4
44-45	0.0	0.0013971207637695582	0.0	0.7887416563900141	1.818170856960384E-4
46-47	0.0	0.0015119526073670562	0.0	0.9321044284613405	1.9617106614572565E-4
48-49	0.0	0.0015263065878167435	0.0	1.0535438877258443	2.1052504659541287E-4
50-51	0.0	0.0016554924118639285	0.0	1.1985765061894842	2.1052504659541287E-4
52-53	0.0	0.00166506173216372	0.0	1.5129382473579347	2.2966368719499585E-4
54-55	0.0	0.0016794157126134072	0.0	2.5217216393815045	2.535869879444746E-4
56-57	0.0	0.0017129083336626775	0.0	3.628389608751641	2.5837164809437035E-4
58-59	0.0	0.0017272623141123646	0.0	4.7542871272642095	2.6794096839416183E-4
60-61	0.0	0.0017655395953115305	0.0	5.5952007179286865	2.8707960899374484E-4
62-63	0.0	0.001799032216360801	0.0	5.6202627677938395	2.966489292935363E-4
64-65	0.0	0.0019521413411574648	0.0	5.634865550571321	2.966489292935363E-4
66-67	0.0	0.001976064641906944	0.0	5.647004233371607	2.966489292935363E-4
68-69	0.0	0.002004772602806318	0.0	5.656889341241293	2.966489292935363E-4
70-71	0.0	0.0020286959035557967	0.0	5.666836649692925	3.062182495933278E-4
72-73	0.0	0.0020286959035557967	0.0	5.67489401738535	3.253568901929108E-4
74-75	0.0	0.0020478345441553797	0.0	5.682932246437175	3.253568901929108E-4
76-77	0.0	0.0021387430870033987	0.0	5.6905350714153595	3.3971087064259807E-4
78-79	0.0	0.0021722357080526692	0.0	5.697381920089859	3.5406485109228526E-4
80-81	0.0	0.0021818050283524608	0.0	5.701649836943567	3.5406485109228526E-4
82-83	0.0	0.0021913743486522523	0.0	5.704802927982348	3.5406485109228526E-4
84-85	0.0	0.0022057283291019394	0.0	5.708046927563977	3.5406485109228526E-4
86-87	0.0	0.0022200823095516265	0.0	5.708501470278218	3.5406485109228526E-4
88-89	0.0	0.002263144250900688	0.0	5.708635440762414	3.5406485109228526E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	16865	0.0	45.388393	94-95
GGTATCA	11855	0.0	44.12085	1
CCGTCTT	7095	0.0	33.632866	92-93
TATGCCG	6810	0.0	32.4597	88-89
GTATCAA	19810	0.0	31.921541	1
TGCCGTC	7360	0.0	31.065655	90-91
GCCGTCT	7195	0.0	30.471485	90-91
ATCTCGT	13495	0.0	29.39839	94-95
ATGCCGT	7240	0.0	29.196634	88-89
CGCTATC	23885	0.0	28.439367	92-93
CGTATGC	8160	0.0	27.440577	86-87
TCGTATG	8220	0.0	25.535736	84-85
TCAACGC	24030	0.0	25.440083	4
CTCGTAT	8730	0.0	25.228731	84-85
ATCAACG	24390	0.0	25.132235	3
CAACGCA	24695	0.0	24.704908	5
TATCAAC	25745	0.0	24.178118	2
AACGCAG	25360	0.0	23.975758	6
GCTATCT	26675	0.0	23.621891	92-93
TCTCGTA	8950	0.0	23.00587	82-83
>>END_MODULE