FastQCFastQC Report
Fri 27 May 2016
SRR2139923_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139923_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14360367
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142250.042.5653151
GTATCAA236350.031.6622751
TCAACGC289750.025.2037564
ATCAACG295450.024.7175123
CAACGCA296050.024.667425
TATCAAC312700.023.687692
AACGCAG308550.023.603076
GTACATG359800.020.3414821
TACATGG358450.020.0934642
ACGCAGA367600.019.7897227
CGCAGAG374500.019.4358188
ACATGGG371800.019.069733
CATGGGG279400.017.2908944
AGAGTAC398000.016.2419210-11
TATACCG10150.015.8128925
GCAGAGT455800.015.7578199
GAGTACT252150.015.49223412-13
GTGGTAT77050.015.1023411
ATGGGGG174800.014.9895695
CAGAGTA437200.014.90496110-11
Produced by FastQC (version 0.11.5)