##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139923_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14360367
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.817887244803703	31.0	31.0	34.0	30.0	34.0
2	31.99258020355608	31.0	31.0	34.0	30.0	34.0
3	32.02279210552209	31.0	31.0	34.0	30.0	34.0
4	35.749423534927764	37.0	35.0	37.0	35.0	37.0
5	35.56695654087392	37.0	35.0	37.0	33.0	37.0
6	35.57345588730427	37.0	35.0	37.0	33.0	37.0
7	35.45978483697527	37.0	35.0	37.0	33.0	37.0
8	35.42817937730979	37.0	35.0	37.0	33.0	37.0
9	37.00747856931512	39.0	37.0	39.0	33.0	39.0
10-11	36.84662105084083	39.0	37.0	39.0	32.5	39.0
12-13	36.80719966975774	39.0	37.0	39.0	32.5	39.0
14-15	37.94457074112381	40.0	37.0	41.0	33.0	41.0
16-17	37.95438835233111	40.0	37.0	41.0	33.0	41.0
18-19	37.924779916836385	40.0	37.0	41.0	33.0	41.0
20-21	37.885861795235506	40.0	37.0	41.0	32.5	41.0
22-23	37.80511075317656	40.0	37.0	41.0	32.0	41.0
24-25	37.70929567868709	39.0	37.0	41.0	32.0	41.0
26-27	37.54779952153234	39.0	36.5	41.0	32.0	41.0
28-29	37.406442048358926	39.0	36.0	40.5	32.0	41.0
30-31	37.27296187452019	39.0	36.0	40.0	31.0	41.0
32-33	37.45659323054403	39.0	36.0	40.5	32.0	41.0
34-35	37.43083578176412	39.0	36.0	41.0	32.0	41.0
36-37	37.32299522825052	39.0	36.0	40.5	31.0	41.0
38-39	37.16074700618062	39.0	36.0	40.0	31.0	41.0
40-41	37.133145963083024	39.0	36.0	40.0	31.0	41.0
42-43	37.00719894992304	39.0	35.5	40.0	31.0	41.0
44-45	37.05336093421026	39.0	35.5	40.0	31.0	41.0
46-47	37.19735341964393	39.0	36.0	40.5	31.0	41.0
48-49	37.127093369544276	39.0	36.0	41.0	31.0	41.0
50-51	37.002045713907904	39.0	35.0	40.0	31.0	41.0
52-53	36.84784467399537	39.0	35.0	40.0	30.5	41.0
54-55	36.699917783614865	39.0	35.0	40.0	30.0	41.0
56-57	36.523306062839744	38.0	35.0	40.0	30.0	41.0
58-59	36.358310514805	38.0	35.0	40.0	30.0	41.0
60-61	36.14514172673755	38.0	34.0	40.0	29.0	41.0
62-63	35.87567328403656	37.5	34.0	40.0	29.0	41.0
64-65	35.633148025520136	37.0	34.0	40.0	29.0	41.0
66-67	35.34656561382702	36.5	34.0	39.0	29.0	41.0
68-69	35.0139667801339	36.0	33.5	39.0	28.0	40.5
70-71	34.659010160687885	36.0	33.0	39.0	28.0	40.0
72-73	34.36484862147843	35.0	33.0	38.0	28.0	40.0
74-75	34.003569302113235	35.0	32.0	37.5	27.5	39.5
76-77	32.9330102198893	34.0	31.0	36.0	26.0	39.0
78-79	33.29102953300527	35.0	32.0	36.0	27.0	39.0
80-81	33.176987640159155	35.0	32.0	36.0	27.0	38.5
82-83	32.750065579343	34.0	31.5	35.5	26.0	37.0
84-85	32.51135449301157	34.0	31.0	35.0	26.0	37.0
86-87	32.42646648681831	34.0	31.0	35.0	26.0	36.0
88-89	32.2719461730825	34.0	31.0	35.0	26.0	36.0
90-91	32.143416131583066	34.0	31.0	35.0	26.0	36.0
92-93	32.06120388154674	34.0	31.0	35.0	26.0	35.0
94-95	32.01034353840387	34.0	31.0	35.0	26.0	35.0
96-97	31.92054224692526	34.0	31.0	35.0	26.0	35.0
98-99	32.130018439696144	34.0	31.0	35.0	26.5	35.0
100-101	31.233415645082722	33.0	29.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	19.0
20	136.0
21	800.0
22	3500.0
23	11364.0
24	29260.0
25	61022.0
26	109150.0
27	172267.0
28	245737.0
29	329477.0
30	422363.0
31	527120.0
32	658111.0
33	831094.0
34	1082227.0
35	1425666.0
36	1978974.0
37	2669483.0
38	2505186.0
39	1256578.0
40	40829.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.0456873421132	21.661974237845037	14.250471453828442	25.041866966213327
2	15.026234678912001	22.627623824780603	39.11470954669057	23.231431949616827
3	17.78076671449507	26.4710959644828	31.036251515891134	24.711885805130997
4	12.799172388781734	18.298579576592964	38.049183426104875	30.853064608520427
5	12.974745004775992	37.95368878803724	35.9807238909702	13.09084231621657
6	30.783106030646714	37.978945802708246	18.05659284334446	13.181355323300581
7	26.407995004584496	32.48667669844371	23.471572836543803	17.633755460427995
8	24.712251434799683	36.710440617569176	21.20904013107743	17.368267816553715
9	25.646635632640862	15.910164412929001	21.070645339356577	37.372554615073554
10-11	24.416593949165787	26.589696488954633	29.09239366932614	19.90131589255344
12-13	24.783092242698253	24.201105027469005	29.237887861779576	21.777914868053163
14-15	22.00682614866319	25.687734860815187	26.448171554389937	25.857267436131682
16-17	21.912652159806978	28.635026697842758	27.313156708889302	22.139164433460962
18-19	21.973505591371463	27.995735702703072	28.700856578080387	21.329902127845077
20-21	22.59019017831171	27.276094667905415	28.49865062669795	21.63506452708493
22-23	22.367859641282966	27.19090751303211	28.36077127589184	22.080461569793083
24-25	22.184657814552867	27.272471724401033	28.4518509998599	22.091019461186203
26-27	22.137136699526057	27.428678084659573	28.371153688062634	22.063031527751733
28-29	22.078717984594665	27.516215441520703	28.378867969736316	22.026198604148323
30-31	22.329741285314704	27.397447880512647	28.25303606875839	22.019774765414247
32-33	22.156840943645502	27.52698606668344	28.189791230403223	22.12638175926784
34-35	22.24729288733635	27.55317915138272	28.24212830294671	21.957399658334214
36-37	22.239809397832403	27.49683664767592	28.199540365793403	22.063813588698274
38-39	22.097006915954243	27.561490995625082	28.28280526907433	22.058696819346345
40-41	22.28320936355359	27.43222857984744	28.14471793407012	22.139844122528856
42-43	22.180412034165517	27.577443697775717	27.951165774972438	22.29097849308633
44-45	22.248026931854554	27.577899360573166	27.839484745668518	22.334588961903766
46-47	22.289025801374063	27.574846822271116	27.681760280681463	22.454367095673362
48-49	22.19975236932763	27.725858118248546	27.65517706246614	22.419212449957683
50-51	22.24176383865023	27.788752081322	27.55064861341556	22.418835466612204
52-53	22.344089446960027	27.660383586802652	27.4472676855105	22.54825928072682
54-55	22.264233681917002	27.53387514001246	27.5215342901553	22.680356887915238
56-57	22.241027452208737	27.604711686286045	27.559029919679357	22.595230941825864
58-59	22.275971936985343	27.60910822089079	27.50929523149297	22.605624610630898
60-61	22.319393649853073	27.548965104494282	27.557437930791934	22.574203314860707
62-63	22.276121548171915	27.703389457851458	27.471368602216717	22.54912039175991
64-65	22.234225985506733	27.710689660182332	27.396529454827757	22.65855489948318
66-67	22.28835348070886	27.821690105097524	27.199478186807706	22.690478227385906
68-69	22.282914970050744	27.800582012574772	27.181235081877553	22.735267935496932
70-71	22.206412907068163	27.60551080879454	27.325635332822294	22.862440951315
72-73	22.308399664723677	27.578082260531232	27.18737205861535	22.92614601612974
74-75	22.24619291513867	27.658070657876543	27.171784515054803	22.923951911929986
76-77	22.520737007615484	27.602904289681714	27.077146904768977	22.799211797933825
78-79	22.458761666793432	27.569104753542256	27.016332151758625	22.955801427905687
80-81	22.473383990257048	27.577811697534244	27.010807541845512	22.937996770363196
82-83	22.376883523387082	27.61637273367446	27.10139371858619	22.905350024352266
84-85	22.54170581230869	27.610176373059563	27.108683138380258	22.739434676251484
86-87	22.425956437629427	27.573603159242328	27.181209214869227	22.81923118825902
88-89	22.39354531798477	27.576031342086633	27.211300616727673	22.819122723200923
90-91	22.484341595935447	27.534796958985368	27.30055163486163	22.680309810217555
92-93	22.497859404044913	27.47542786648769	27.354260879105478	22.67245185036192
94-95	22.446471726904978	27.448611647771965	27.467208928762542	22.637707696560515
96-97	22.21040237837013	27.44333747624968	27.653281814345554	22.692978331034634
98-99	22.267066490269784	28.07161657455345	27.814389345642272	21.846927589534495
100-101	25.172789335482083	35.83866550997505	28.97807954148161	10.010465613061255
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2703.0
1	2510.5
2	3456.5
3	4925.0
4	6690.5
5	10465.0
6	13686.5
7	16208.0
8	17760.5
9	17571.5
10	16568.0
11	15513.5
12	17108.0
13	22513.5
14	34605.5
15	51169.0
16	63937.5
17	69855.0
18	71130.5
19	70184.5
20	68291.5
21	67028.5
22	68584.0
23	76139.5
24	90755.0
25	111675.0
26	137987.0
27	166307.0
28	197623.5
29	230829.0
30	262582.0
31	294431.5
32	326217.5
33	356969.5
34	386365.0
35	415537.0
36	448323.5
37	479120.0
38	505114.0
39	527790.5
40	549336.5
41	572249.5
42	590500.0
43	605676.0
44	620234.0
45	632241.0
46	639580.5
47	642575.5
48	642357.0
49	636791.0
50	626458.5
51	612145.0
52	590802.0
53	563859.0
54	533271.5
55	497135.0
56	458689.5
57	418213.5
58	373682.5
59	327141.0
60	279781.5
61	232227.5
62	187835.5
63	148371.5
64	113325.0
65	86086.0
66	65567.0
67	50168.0
68	38537.5
69	28383.5
70	20683.5
71	15218.0
72	11066.5
73	7934.0
74	5652.5
75	3993.0
76	2753.5
77	1881.0
78	1296.5
79	900.0
80	636.0
81	429.5
82	299.0
83	214.0
84	163.5
85	125.5
86	99.0
87	82.5
88	60.5
89	42.5
90	27.5
91	15.0
92	12.0
93	9.5
94	7.0
95	4.5
96	3.0
97	2.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0012255954182786553
3	1.3927220662257447E-5
4	6.963610331128724E-6
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0015180670521860618
18-19	0.0014275401178813884
20-21	1.7409917517730174E-5
22-23	0.002535176267184177
24-25	1.2539902142783644E-4
26-27	0.0012684665114560821
28-29	5.543739758027962E-4
30-31	1.466600734815855E-4
32-33	0.0010579156259742806
34-35	0.016308035923710346
36-37	0.03374166906214919
38-39	0.007506942043760858
40-41	0.00449192354673906
42-43	0.007544025795823551
44-45	0.00532069884127742
46-47	0.003039159438201447
48-49	0.0013526564615069308
50-51	1.501159299137207E-4
52-53	8.961644977732455E-5
54-55	0.004537914890006621
56-57	0.011956373975723647
58-59	0.00990415572297967
60-61	0.008065184117013407
62-63	0.0018982519435909611
64-65	0.0012537786495304493
66-67	0.0014387390925054099
68-69	0.0
70-71	4.46450674723137E-6
72-73	6.137057402807308E-4
74-75	8.564562636026086E-4
76-77	6.604513788903889E-5
78-79	8.947963407447967E-4
80-81	0.002746147545545889
82-83	0.005014262288581586
84-85	0.013278954221343658
86-87	0.008613333513673365
88-89	0.07857506053034702
90-91	0.0
92-93	0.0
94-95	1.7034751421564692E-4
96-97	0.008904522090921803
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	471.0
20-21	1388.0
22-23	2712.0
24-25	6012.0
26-27	7169.0
28-29	14430.0
30-31	41456.0
32-33	59888.0
34-35	85504.0
36-37	125772.0
38-39	133597.0
40-41	144380.0
42-43	159725.0
44-45	187049.0
46-47	187018.0
48-49	172348.0
50-51	162636.0
52-53	146192.0
54-55	163404.0
56-57	171154.0
58-59	179561.0
60-61	180985.0
62-63	176216.0
64-65	192882.0
66-67	204024.0
68-69	204132.0
70-71	202304.0
72-73	197645.0
74-75	201878.0
76-77	201920.0
78-79	214156.0
80-81	217582.0
82-83	210523.0
84-85	211440.0
86-87	224128.0
88-89	238934.0
90-91	257885.0
92-93	279976.0
94-95	352935.0
96-97	428762.0
98-99	1162200.0
100-101	6647994.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.65489409559018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.070677233753	78.91719890904541
2	6.286206316478091	10.89461085234878
3	1.4961953071478054	3.8895793766163624
4	0.532203842232173	1.8447227034358051
5	0.23503464817631858	1.018345127325659
6	0.11878874551792602	0.6176175697562336
7	0.06895085235852708	0.4182450166250186
8	0.045136313437711885	0.31290259686482297
9	0.030731730103527977	0.23967493357459294
>10	0.11199781539477781	1.532177382225131
>50	0.003110635426469113	0.17973310449332494
>100	9.399009388638492E-4	0.1190336865851011
>500	2.665903481069601E-5	0.016158741103790782
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.089083099338617E-5
2	0.0	2.7854441324514894E-5	0.0	0.0	2.089083099338617E-5
3	0.0	2.7854441324514894E-5	0.0	0.0	2.089083099338617E-5
4	0.0	5.570888264902979E-5	0.0	1.3927220662257447E-5	2.7854441324514894E-5
5	0.0	5.570888264902979E-5	0.0	2.089083099338617E-5	2.7854441324514894E-5
6	2.7854441324514894E-5	5.570888264902979E-5	0.0	2.7854441324514894E-5	3.481805165564362E-5
7	2.7854441324514894E-5	6.267249298015851E-5	0.0	2.7854441324514894E-5	4.178166198677234E-5
8	2.7854441324514894E-5	6.267249298015851E-5	0.0	2.7854441324514894E-5	4.8745272317901066E-5
9	2.7854441324514894E-5	1.2534498596031702E-4	0.0	6.963610331128724E-5	5.570888264902979E-5
10-11	2.7854441324514894E-5	1.2534498596031702E-4	0.0	9.400873947023778E-5	5.570888264902979E-5
12-13	2.7854441324514894E-5	1.357904014570101E-4	0.0	1.183813756291883E-4	6.267249298015851E-5
14-15	3.481805165564362E-5	1.8453567377491118E-4	0.0	1.3230859629144575E-4	9.749054463580213E-5
16-17	8.356332397354469E-5	1.9149928410603989E-4	0.0	1.6364484278152502E-4	1.0793596013249521E-4
18-19	8.356332397354469E-5	2.1587192026499043E-4	0.0	2.2631733576168353E-4	1.3230859629144575E-4
20-21	8.704512913910905E-5	2.2979914092724789E-4	0.0	3.8996217854320853E-4	1.3230859629144575E-4
22-23	9.052693430467342E-5	2.8898982874184205E-4	0.0	8.913421223844766E-4	1.566812324503963E-4
24-25	9.052693430467342E-5	3.307714907286144E-4	0.0	0.002026410606358459	1.8801747894047553E-4
26-27	9.052693430467342E-5	3.481805165564362E-4	0.0	0.005543033823578464	1.8801747894047553E-4
28-29	9.052693430467342E-5	3.481805165564362E-4	0.0	0.02829663058054157	1.9498108927160426E-4
30-31	9.052693430467342E-5	4.0040759403990166E-4	0.0	0.04664922560823132	1.9846289443716864E-4
32-33	9.052693430467342E-5	4.03889399205466E-4	0.0	0.06646069700029254	2.1587192026499045E-4
34-35	9.749054463580213E-5	4.700436973511889E-4	0.0	0.09626843102268903	2.193537254305548E-4
36-37	9.749054463580213E-5	5.431616058280404E-4	0.0	0.137325877535024	2.2979914092724789E-4
38-39	9.749054463580213E-5	5.67534241986991E-4	0.0	0.1991000647824669	2.576535822517628E-4
40-41	9.749054463580213E-5	6.336885401327139E-4	0.0	0.29575497617853364	2.715808029140202E-4
42-43	1.009723498013665E-4	6.510975659605357E-4	0.0	0.3468504669831906	2.7854441324514896E-4
44-45	1.0445415496693086E-4	7.520699157619022E-4	0.0	0.42411172360706384	2.8550802357627767E-4
46-47	1.0445415496693086E-4	8.321514345698825E-4	0.0	0.5039286252224613	2.994352442385351E-4
48-49	1.0445415496693086E-4	8.530422655632687E-4	0.0	0.5730180851227549	3.0639885456966383E-4
50-51	1.2534498596031702E-4	9.261601740401203E-4	0.0	0.6420587997507305	3.098806597352282E-4
52-53	1.3230859629144575E-4	9.33123784371249E-4	0.0	0.7197169821634781	3.3425329589417875E-4
54-55	1.3230859629144575E-4	9.470510050335065E-4	0.0	0.8868262210847397	3.586259320531293E-4
56-57	1.3230859629144575E-4	9.609782256957639E-4	0.0	1.0383160820332795	3.6907134754982237E-4
58-59	1.3230859629144575E-4	9.888326670202789E-4	0.0	1.1621186283052514	3.7951676304651545E-4
60-61	1.357904014570101E-4	9.992780825169717E-4	0.0	1.231497078034287	3.829985682120798E-4
62-63	1.462358169537032E-4	0.0010132053031792292	0.0	1.2698108620761572	3.8996217854320853E-4
64-65	1.462358169537032E-4	0.0010863232116560808	0.0	1.29721963233948	3.8996217854320853E-4
66-67	1.462358169537032E-4	0.0010863232116560808	0.0	1.310669845694055	3.8996217854320853E-4
68-69	1.462358169537032E-4	0.0011141776529805959	0.0	1.322354783829689	3.934439837087729E-4
70-71	1.462358169537032E-4	0.0011385502891395464	0.0	1.3334199606458526	4.1781661986772345E-4
72-73	1.6712664794708937E-4	0.001152477509801804	0.0	1.3422811547922138	4.665618921856245E-4
74-75	1.6712664794708937E-4	0.001183813756291883	0.0	1.351351257248509	4.665618921856245E-4
76-77	1.6712664794708937E-4	0.001232559028609784	0.0	1.359279327610499	4.665618921856245E-4
78-79	1.740902582782181E-4	0.0012813043009276852	0.0	1.3668801082869262	4.665618921856245E-4
80-81	1.740902582782181E-4	0.0012813043009276852	0.0	1.3713194098730206	4.665618921856245E-4
82-83	1.810538686093468E-4	0.0012917497164243782	0.0	1.3743799166135517	4.8048911284788193E-4
84-85	1.8801747894047553E-4	0.0013161223525833287	0.0	1.3773951598869303	4.839709180134463E-4
86-87	1.9498108927160426E-4	0.0013300495732455862	0.0	1.377799049286136	4.874527231790107E-4
88-89	2.2283553059611916E-4	0.0013718312352323586	0.0	1.377861721779116	4.874527231790107E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14225	0.0	42.565315	1
GTATCAA	23635	0.0	31.662275	1
TCAACGC	28975	0.0	25.203756	4
ATCAACG	29545	0.0	24.717512	3
CAACGCA	29605	0.0	24.66742	5
TATCAAC	31270	0.0	23.68769	2
AACGCAG	30855	0.0	23.60307	6
GTACATG	35980	0.0	20.341482	1
TACATGG	35845	0.0	20.093464	2
ACGCAGA	36760	0.0	19.789722	7
CGCAGAG	37450	0.0	19.435818	8
ACATGGG	37180	0.0	19.06973	3
CATGGGG	27940	0.0	17.290894	4
AGAGTAC	39800	0.0	16.24192	10-11
TATACCG	1015	0.0	15.812892	5
GCAGAGT	45580	0.0	15.757819	9
GAGTACT	25215	0.0	15.492234	12-13
GTGGTAT	7705	0.0	15.102341	1
ATGGGGG	17480	0.0	14.989569	5
CAGAGTA	43720	0.0	14.904961	10-11
>>END_MODULE