FastQCFastQC Report
Fri 27 May 2016
SRR2139922_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139922_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15053027
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150000.044.75191
GTATCAA252300.031.2597031
TCAACGC309650.024.7218914
ATCAACG315600.024.2811163
CAACGCA320100.023.9272965
TATCAAC332650.023.3489342
AACGCAG330250.023.0468046
GTACATG382650.020.2144951
ACGCAGA391600.019.4259837
TACATGG389350.019.4256152
CGCAGAG400300.018.9738568
ACATGGG397550.018.5124653
GTGGTAT76200.017.3987521
CATGGGG308350.016.3176364
GAGTACT265400.015.97231212-13
AGAGTAC424500.015.95928110-11
GCAGAGT481200.015.6345719
TGGTATC76750.015.2969172
CAGAGTA464550.015.15933610-11
GTACTTT303400.013.98600914-15
Produced by FastQC (version 0.11.5)