##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139922_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15053027
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.793960377537356	31.0	31.0	34.0	30.0	34.0
2	31.97371990364463	31.0	31.0	34.0	30.0	34.0
3	32.000631168734365	31.0	31.0	34.0	30.0	34.0
4	35.73367795062083	37.0	35.0	37.0	35.0	37.0
5	35.54530746540214	37.0	35.0	37.0	33.0	37.0
6	35.55573646416764	37.0	35.0	37.0	33.0	37.0
7	35.4404897433586	37.0	35.0	37.0	33.0	37.0
8	35.410563602921854	37.0	35.0	37.0	33.0	37.0
9	36.98433464578254	39.0	37.0	39.0	33.0	39.0
10-11	36.82105868142003	39.0	37.0	39.0	32.5	39.0
12-13	36.77998265730872	39.0	37.0	39.0	32.0	39.0
14-15	37.91172855134054	40.0	37.0	41.0	33.0	41.0
16-17	37.9219034816054	40.0	37.0	41.0	33.0	41.0
18-19	37.89132810297889	40.0	37.0	41.0	32.5	41.0
20-21	37.85156264773248	40.0	37.0	41.0	32.0	41.0
22-23	37.77029532594939	39.5	37.0	41.0	32.0	41.0
24-25	37.6724998440401	39.0	37.0	41.0	32.0	41.0
26-27	37.50881811259471	39.0	36.0	41.0	32.0	41.0
28-29	37.365090201891974	39.0	36.0	40.5	32.0	41.0
30-31	37.2309718717335	39.0	36.0	40.0	31.0	41.0
32-33	37.408938280797	39.0	36.0	40.5	32.0	41.0
34-35	37.3813930586667	39.0	36.0	41.0	31.5	41.0
36-37	37.27328167755785	39.0	36.0	40.5	31.0	41.0
38-39	37.1082483628402	39.0	36.0	40.0	31.0	41.0
40-41	37.07736583372757	39.0	36.0	40.0	31.0	41.0
42-43	36.94869229850404	39.0	35.0	40.0	31.0	41.0
44-45	36.99386869631819	39.0	35.5	40.0	31.0	41.0
46-47	37.13872357299636	39.0	36.0	40.0	31.0	41.0
48-49	37.06495075741258	39.0	35.5	40.0	31.0	41.0
50-51	36.93959873863136	39.0	35.0	40.0	31.0	41.0
52-53	36.78095809201814	39.0	35.0	40.0	30.0	41.0
54-55	36.6282760499538	38.5	35.0	40.0	30.0	41.0
56-57	36.444264380633435	38.0	35.0	40.0	30.0	41.0
58-59	36.27483840872222	38.0	34.5	40.0	30.0	41.0
60-61	36.05425466301713	38.0	34.0	40.0	29.0	41.0
62-63	35.77695274787499	37.5	34.0	40.0	29.0	41.0
64-65	35.5271300006135	37.0	34.0	40.0	29.0	41.0
66-67	35.232902773492825	36.5	34.0	39.0	28.0	41.0
68-69	34.893644709921745	36.0	33.0	39.0	28.0	40.0
70-71	34.532904421064224	35.5	33.0	38.5	28.0	40.0
72-73	34.24028621340197	35.0	33.0	38.0	28.0	40.0
74-75	33.87985207362654	35.0	32.0	37.0	27.5	39.0
76-77	32.819406090431045	34.0	31.0	36.0	26.0	39.0
78-79	33.179351635870674	34.5	32.0	36.0	26.5	39.0
80-81	33.06541739598474	34.0	32.0	36.0	27.0	37.5
82-83	32.64760085749968	34.0	31.5	35.5	26.0	37.0
84-85	32.4126320042915	34.0	31.0	35.0	26.0	37.0
86-87	32.32601889582941	34.0	31.0	35.0	26.0	36.0
88-89	32.1739456944795	34.0	31.0	35.0	26.0	36.0
90-91	32.052263207136164	34.0	31.0	35.0	26.0	35.5
92-93	31.97427244926548	34.0	31.0	35.0	26.0	35.0
94-95	31.92652916594181	34.0	31.0	35.0	26.0	35.0
96-97	31.83560067478811	34.0	31.0	35.0	26.0	35.0
98-99	32.05406986359388	34.0	31.0	35.0	26.5	35.0
100-101	31.142200640312993	33.0	29.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	0.0
15	0.0
16	0.0
17	0.0
18	9.0
19	45.0
20	174.0
21	916.0
22	3809.0
23	12525.0
24	32311.0
25	66609.0
26	117966.0
27	183896.0
28	262160.0
29	350760.0
30	449133.0
31	562129.0
32	703190.0
33	890030.0
34	1153781.0
35	1524473.0
36	2112786.0
37	2805153.0
38	2531761.0
39	1253504.0
40	35905.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.09028396747046	21.558906391385598	14.233761754363425	25.117047886780515
2	15.22499321391146	22.611532670150126	38.63751020238421	23.5259639135542
3	17.787167842532124	26.278842539699326	31.133640505237953	24.800349112530597
4	12.962505936515209	18.19037279090054	37.684138262460635	31.16298301012361
5	13.069922747099305	37.875159594146744	35.827644499674385	13.227273159079566
6	31.11447285652248	37.6998128017707	17.858022841518853	13.327691500187969
7	26.56774614168964	32.429603693662415	23.27864687946152	17.72400328518643
8	25.04263760371917	36.3959155856161	21.06109953831877	17.500347272345955
9	25.791643102746047	15.644979577861648	20.871582838455016	37.69179448093729
10-11	24.596315412175905	26.390203777618947	28.972352205307278	20.041128604897875
12-13	24.89943384808916	24.046296469142053	29.099346596535035	21.95492308623375
14-15	22.19106163830039	25.498516012759424	26.25506484509727	26.055357503842913
16-17	22.117711295404476	28.423174354399695	27.129708389369373	22.329405960826456
18-19	22.180923611491483	27.784200894801632	28.529505757171897	21.505369736534984
20-21	22.791126785135113	27.06607037818071	28.324353042361455	21.818449794322724
22-23	22.600582444524814	26.935893162114038	28.195174131696994	22.268350261664153
24-25	22.38222441102859	27.080982457259704	28.25538119874035	22.28141193297135
26-27	22.36517598008059	27.175839649464763	28.21950488586613	22.239479484588514
28-29	22.313505102953197	27.270422989474756	28.220923199170585	22.195148708401458
30-31	22.531046243073657	27.14124345964599	28.083454918839657	22.244255378440698
32-33	22.332722770941615	27.324918651579495	28.03926628607397	22.30309229140492
34-35	22.452535783536955	27.299578440454475	28.071496208605158	22.176389567403408
36-37	22.441906668687835	27.25604517760507	28.0438848781073	22.258163275599788
38-39	22.31901733004982	27.34800852439515	28.08978306787651	22.243191077678517
40-41	22.510064789351567	27.218233029110216	27.956970163999838	22.31473201753838
42-43	22.41575126945861	27.371646034781737	27.73089373440685	22.48170896135281
44-45	22.476213654997224	27.380733100344212	27.63125978440223	22.511793460256335
46-47	22.55566324572375	27.337892939123414	27.481739910760165	22.62470390439267
48-49	22.47158581383629	27.497362520709	27.41927987527174	22.61177179018297
50-51	22.480276215137817	27.60091542508248	27.34992487451857	22.568883485261136
52-53	22.655799591673105	27.4428637621419	27.200570905162262	22.700765741022728
54-55	22.542007992236922	27.314964518781558	27.31760079564048	22.82542669334104
56-57	22.503466046269967	27.39483752256191	27.368370825132043	22.733325606036082
58-59	22.52921066506528	27.405354919635982	27.295551847254586	22.769882568044153
60-61	22.590615143512807	27.336838459967773	27.392536557761808	22.680009838757606
62-63	22.553041120372622	27.464671421798354	27.304714251645194	22.677573206183833
64-65	22.457751542591787	27.459573843491647	27.303538054105736	22.779136559810837
66-67	22.510940961031892	27.53559393898125	27.111989206907456	22.8414758930794
68-69	22.458994854844427	27.582186667883356	27.12044496998624	22.83837350728598
70-71	22.434165082803347	27.350751627656745	27.216368135644505	22.998715153895407
72-73	22.480456934003477	27.34016586573918	27.120986952768078	23.058390247489267
74-75	22.367462279653523	27.383587495127266	27.134431961177725	23.11451826404149
76-77	22.61340021774734	27.272624719713413	27.06803683282467	23.045938229714576
78-79	22.55394568185183	27.2752282779901	27.003244588289594	23.167581451868475
80-81	22.581766455328136	27.34256147985138	26.951018653300185	23.1246534115203
82-83	22.492041012606734	27.307750562099177	27.017330840568494	23.1828775847256
84-85	22.586052051204284	27.31617830141378	27.036921227103157	23.06084842027878
86-87	22.500427648562937	27.31043361870374	27.122359266963592	23.066779465769734
88-89	22.46207051956368	27.214513987232163	27.201233271520813	23.122182221683342
90-91	22.446795529274414	27.154235436837688	27.29470228231097	23.104266751576937
92-93	22.406265125123486	27.034774146923173	27.430163676404813	23.12879705154853
94-95	22.252877096255194	27.116818642982597	27.52822370036573	23.10208056039648
96-97	22.10621873057372	27.159320615509415	27.619017878573597	23.11544277534327
98-99	22.190173787920905	27.84061463573339	27.70782884404803	22.261382732297673
100-101	25.28903171404403	35.61309410358226	28.868913505412912	10.2289606769608
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3644.0
1	3300.0
2	4158.0
3	5744.0
4	7485.5
5	11012.0
6	14192.0
7	17223.0
8	19509.0
9	19743.5
10	18698.0
11	17544.0
12	19145.5
13	24614.0
14	37487.5
15	54962.0
16	68114.0
17	74101.0
18	75350.5
19	74344.0
20	72079.5
21	70789.0
22	72704.0
23	80638.5
24	95477.5
25	117223.0
26	144398.5
27	172461.5
28	202112.0
29	233037.5
30	262072.0
31	291850.0
32	323974.5
33	355308.5
34	385001.0
35	415330.5
36	450056.5
37	483152.5
38	510051.0
39	533806.0
40	557414.0
41	581538.0
42	601534.0
43	620581.5
44	638906.0
45	654176.0
46	665398.5
47	672395.0
48	675812.5
49	674164.0
50	668500.0
51	658709.0
52	640877.5
53	616956.5
54	589149.0
55	554711.5
56	516353.0
57	473390.5
58	425112.5
59	373638.0
60	320572.0
61	267532.5
62	217930.5
63	172408.0
64	131574.5
65	100049.0
66	75928.5
67	57962.0
68	44802.0
69	33188.0
70	24161.0
71	17935.5
72	12964.5
73	9107.0
74	6319.0
75	4420.0
76	3076.0
77	2094.0
78	1479.0
79	1045.0
80	737.0
81	518.5
82	360.0
83	245.5
84	179.0
85	128.5
86	92.0
87	72.5
88	45.0
89	25.5
90	19.0
91	15.0
92	12.5
93	9.0
94	5.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0011492705088484861
3	6.643182132072174E-6
4	2.6572728528288697E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0014282841583955173
18-19	0.0012854557425559657
20-21	2.325237553742468E-5
22-23	0.0024650527282087348
24-25	1.4621559642554707E-4
26-27	0.0011569475866173079
28-29	4.7567195732524E-4
30-31	1.4659873769824188E-4
32-33	8.959604386662424E-4
34-35	0.015759922922520642
36-37	0.03356956788724307
38-39	0.007699950068233245
40-41	0.004564415923942053
42-43	0.007380850672717055
44-45	0.005228097323118664
46-47	0.0030730064848693743
48-49	0.0013731750294422203
50-51	2.0675848441748068E-4
52-53	1.1968020103880171E-4
54-55	0.004586759831043615
56-57	0.012027473191910838
58-59	0.009875182217376688
60-61	0.008189062427121795
62-63	0.0018879096618356131
64-65	0.0013054145413226117
66-67	0.0012697399435662837
68-69	0.0
70-71	0.0
72-73	7.493165116012381E-4
74-75	8.926386854788484E-4
76-77	8.187693596309316E-5
78-79	8.768202951701861E-4
80-81	0.002610375289533731
82-83	0.0050581405423468975
84-85	0.012895493741902922
86-87	0.008610378889029558
88-89	0.07655687178469556
90-91	0.0
92-93	5.479496491259217E-6
94-95	1.758041238089767E-4
96-97	0.008655085750351116
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	511.0
20-21	1513.0
22-23	3008.0
24-25	6462.0
26-27	7892.0
28-29	16340.0
30-31	46396.0
32-33	66802.0
34-35	95796.0
36-37	139266.0
38-39	149476.0
40-41	157151.0
42-43	177145.0
44-45	205274.0
46-47	204391.0
48-49	188250.0
50-51	177273.0
52-53	163803.0
54-55	182772.0
56-57	185849.0
58-59	189163.0
60-61	195604.0
62-63	186745.0
64-65	202985.0
66-67	214260.0
68-69	214739.0
70-71	212189.0
72-73	207162.0
74-75	210566.0
76-77	211215.0
78-79	222913.0
80-81	224930.0
82-83	218701.0
84-85	220224.0
86-87	232424.0
88-89	248427.0
90-91	270323.0
92-93	293496.0
94-95	376291.0
96-97	452309.0
98-99	1219363.0
100-101	6853628.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.99335786947572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.37794661482097	79.49274411240968
2	6.060387477155698	10.544269132557895
3	1.4154787662927657	3.6941175261825165
4	0.5165559600958887	1.7974774998492906
5	0.2391039592312698	1.0400228146707193
6	0.11997012992387864	0.6261962667669283
7	0.07686860645504666	0.4680940733190236
8	0.048155842010661525	0.3351390718031529
9	0.033872367172869754	0.2652003863420166
>10	0.10835510853161462	1.4928863960251975
>50	0.002712811540325883	0.1540520953799866
>100	5.672734786624959E-4	0.07433073228732573
>500	2.5083290362331096E-5	0.015469892406353393
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.643182132072174E-6	0.0
3	0.0	0.0	0.0	6.643182132072174E-6	0.0
4	0.0	1.9929546396216522E-5	0.0	1.3286364264144348E-5	0.0
5	0.0	3.321591066036087E-5	0.0	1.9929546396216522E-5	0.0
6	0.0	3.321591066036087E-5	0.0	1.9929546396216522E-5	6.643182132072174E-6
7	0.0	3.321591066036087E-5	0.0	2.6572728528288697E-5	6.643182132072174E-6
8	0.0	4.6502274924505216E-5	0.0	2.6572728528288697E-5	1.9929546396216522E-5
9	0.0	1.0629091411315479E-4	0.0	2.6572728528288697E-5	1.9929546396216522E-5
10-11	0.0	1.0629091411315479E-4	0.0	3.321591066036087E-5	1.9929546396216522E-5
12-13	0.0	1.0961250517919087E-4	0.0	3.9859092792433045E-5	1.9929546396216522E-5
14-15	0.0	1.5943637116973218E-4	0.0	3.9859092792433045E-5	2.9894319594324782E-5
16-17	1.3286364264144348E-5	1.5943637116973218E-4	0.0	6.643182132072173E-5	3.9859092792433045E-5
18-19	1.3286364264144348E-5	1.6607955330180435E-4	0.0	1.1957727837729913E-4	3.9859092792433045E-5
20-21	1.3286364264144348E-5	1.7272273543387652E-4	0.0	2.7901364954703134E-4	3.9859092792433045E-5
22-23	1.6607955330180434E-5	2.059386460942374E-4	0.0	6.942125328015422E-4	4.982386599054131E-5
24-25	2.325113746225261E-5	2.3915455675459826E-4	0.0	0.0017272273543387652	5.9788639188649564E-5
26-27	2.6572728528288697E-5	2.590841031508148E-4	0.0	0.005337796843119992	5.9788639188649564E-5
28-29	2.6572728528288697E-5	2.590841031508148E-4	0.0	0.03060846167352254	5.9788639188649564E-5
30-31	2.6572728528288697E-5	3.1555115127342826E-4	0.0	0.044851444164685283	6.311023025468565E-5
32-33	2.6572728528288697E-5	3.255159244715365E-4	0.0	0.05132190356132358	6.643182132072173E-5
34-35	2.6572728528288697E-5	3.9526933685829436E-4	0.0	0.06106413015800742	7.307500345279392E-5
36-37	2.6572728528288697E-5	4.6834434031108827E-4	0.0	0.07594817972491513	7.307500345279392E-5
38-39	2.6572728528288697E-5	4.783091135091965E-4	0.0	0.10268698780650563	7.307500345279392E-5
40-41	2.6572728528288697E-5	5.181682063016296E-4	0.0	0.15577597781496041	7.307500345279392E-5
42-43	2.6572728528288697E-5	5.281329794997378E-4	0.0	0.1785189118441095	7.639659451883E-5
44-45	2.9894319594324782E-5	5.77956845490279E-4	0.0	0.21313985552540363	8.303977665090218E-5
46-47	3.321591066036087E-5	6.178159382827121E-4	0.0	0.2536267290293175	8.968295878297435E-5
48-49	3.321591066036087E-5	6.244591204147843E-4	0.0	0.2876929670025836	9.96477319810826E-5
50-51	3.321591066036087E-5	7.141420791977587E-4	0.0	0.32514058468107443	9.96477319810826E-5
52-53	3.321591066036087E-5	7.174636702637948E-4	0.0	0.36901548107234516	1.0961250517919086E-4
54-55	3.321591066036087E-5	7.274284434619031E-4	0.0	0.4939239131106322	1.1957727837729913E-4
56-57	3.653750172639695E-5	7.440363987920835E-4	0.0	0.5978066737009108	1.1957727837729913E-4
58-59	3.9859092792433045E-5	7.440363987920835E-4	0.0	0.7075487209316771	1.1957727837729913E-4
60-61	4.6502274924505216E-5	7.639659451882999E-4	0.0	0.7692705261207596	1.1957727837729913E-4
62-63	4.6502274924505216E-5	7.739307183864082E-4	0.0	0.7975870899587173	1.262204605093713E-4
64-65	4.6502274924505216E-5	8.4036253970713E-4	0.0	0.8261095924427692	1.262204605093713E-4
66-67	4.982386599054131E-5	8.436841307731661E-4	0.0	0.8402462840198188	1.2954205157540737E-4
68-69	5.314545705657739E-5	8.503273129052383E-4	0.0	0.8518319936581527	1.3286364264144347E-4
70-71	5.314545705657739E-5	8.569704950373104E-4	0.0	0.8628032089492699	1.3286364264144347E-4
72-73	5.9788639188649564E-5	8.569704950373104E-4	0.0	0.8719076900612748	1.5943637116973218E-4
74-75	5.9788639188649564E-5	8.868648146316352E-4	0.0	0.8807530870701288	1.5943637116973218E-4
76-77	6.643182132072173E-5	9.532966359523569E-4	0.0	0.8891234965565398	1.6275796223576825E-4
78-79	6.643182132072173E-5	9.765477734146096E-4	0.0	0.8969989889741112	1.6607955330180435E-4
80-81	6.643182132072173E-5	9.765477734146096E-4	0.0	0.9020079483016937	1.6607955330180435E-4
82-83	6.643182132072173E-5	9.831909555466818E-4	0.0	0.9054524382371731	1.6607955330180435E-4
84-85	6.643182132072173E-5	9.831909555466818E-4	0.0	0.9086677383890962	1.6607955330180435E-4
86-87	7.307500345279392E-5	9.831909555466818E-4	0.0	0.909122796365143	1.6607955330180435E-4
88-89	7.307500345279392E-5	9.96477319810826E-4	0.0	0.9092257656881901	1.7272273543387652E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15000	0.0	44.7519	1
GTATCAA	25230	0.0	31.259703	1
TCAACGC	30965	0.0	24.721891	4
ATCAACG	31560	0.0	24.281116	3
CAACGCA	32010	0.0	23.927296	5
TATCAAC	33265	0.0	23.348934	2
AACGCAG	33025	0.0	23.046804	6
GTACATG	38265	0.0	20.214495	1
ACGCAGA	39160	0.0	19.425983	7
TACATGG	38935	0.0	19.425615	2
CGCAGAG	40030	0.0	18.973856	8
ACATGGG	39755	0.0	18.512465	3
GTGGTAT	7620	0.0	17.398752	1
CATGGGG	30835	0.0	16.317636	4
GAGTACT	26540	0.0	15.972312	12-13
AGAGTAC	42450	0.0	15.959281	10-11
GCAGAGT	48120	0.0	15.634571	9
TGGTATC	7675	0.0	15.296917	2
CAGAGTA	46455	0.0	15.159336	10-11
GTACTTT	30340	0.0	13.986009	14-15
>>END_MODULE