FastQCFastQC Report
Fri 27 May 2016
SRR2139921_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139921_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14621190
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154450.043.0047951
GTATCAA249800.029.9717181
TCAACGC301500.023.9112574
ATCAACG304650.023.730053
CAACGCA307300.023.4206835
TATCAAC321600.022.729852
AACGCAG318500.022.5592046
GTACATG360300.019.8529571
TACATGG360100.019.2718622
ACGCAGA380600.018.8149477
CGCAGAG385900.018.556548
ACATGGG365300.018.4135843
GTGGTAT77000.016.1445311
CATGGGG269600.016.0562524
AGAGTAC412050.015.85597810-11
GAGTACT268300.015.54237812-13
GCAGAGT464200.015.2358079
CAGAGTA450650.015.01116710-11
TGGTATC77550.014.7331052
TATACTG75750.013.914595
Produced by FastQC (version 0.11.5)