##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139921_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14621190
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.823577355878694	31.0	31.0	34.0	30.0	34.0
2	32.00335047968052	31.0	31.0	34.0	30.0	34.0
3	32.03367010482731	33.0	31.0	34.0	30.0	34.0
4	35.75995346480006	37.0	35.0	37.0	35.0	37.0
5	35.58400991984921	37.0	35.0	37.0	33.0	37.0
6	35.59000847400246	37.0	35.0	37.0	33.0	37.0
7	35.47865973973391	37.0	35.0	37.0	33.0	37.0
8	35.448475124117806	37.0	35.0	37.0	33.0	37.0
9	37.027741654407066	39.0	37.0	39.0	33.0	39.0
10-11	36.87169392504988	39.0	37.0	39.0	33.0	39.0
12-13	36.83332355300766	39.0	37.0	39.0	32.5	39.0
14-15	37.976944455273475	40.0	37.0	41.0	33.0	41.0
16-17	37.98744780691585	40.0	37.0	41.0	33.0	41.0
18-19	37.95951075117689	40.0	37.0	41.0	33.0	41.0
20-21	37.92290629829433	40.0	37.0	41.0	33.0	41.0
22-23	37.843119721643106	40.0	37.0	41.0	32.5	41.0
24-25	37.74993269372568	39.5	37.0	41.0	32.0	41.0
26-27	37.592003073740635	39.0	37.0	41.0	32.0	41.0
28-29	37.450021763995714	39.0	36.0	41.0	32.0	41.0
30-31	37.31920877778307	39.0	36.0	40.0	31.5	41.0
32-33	37.496101104609224	39.0	36.0	40.5	32.0	41.0
34-35	37.46828848240831	39.0	36.0	41.0	32.0	41.0
36-37	37.36155547140659	39.0	36.0	40.5	31.5	41.0
38-39	37.19949189341264	39.0	36.0	40.0	31.0	41.0
40-41	37.17551485006871	39.0	36.0	40.0	31.0	41.0
42-43	37.052593249412936	39.0	36.0	40.0	31.0	41.0
44-45	37.104845347796555	39.0	35.5	40.0	31.0	41.0
46-47	37.25746409289523	39.0	36.0	41.0	31.0	41.0
48-49	37.194403454625046	39.0	36.0	41.0	31.0	41.0
50-51	37.07745159985326	39.0	35.5	40.5	31.0	41.0
52-53	36.931727624818876	39.0	35.0	40.0	31.0	41.0
54-55	36.791760539515025	39.0	35.0	40.0	30.5	41.0
56-57	36.62617532358463	38.5	35.0	40.0	30.0	41.0
58-59	36.47217697211812	38.0	35.0	40.0	30.0	41.0
60-61	36.26391165463109	38.0	34.5	40.0	30.0	41.0
62-63	35.99609706718397	38.0	34.0	40.0	29.0	41.0
64-65	35.755011543973595	37.0	34.0	40.0	29.0	41.0
66-67	35.468957008943214	37.0	34.0	39.5	29.0	41.0
68-69	35.13955922771993	36.0	34.0	39.0	28.5	40.5
70-71	34.786862024478324	36.0	33.0	39.0	28.0	40.0
72-73	34.48848018035903	35.0	33.0	38.5	28.0	40.0
74-75	34.12785037535491	35.0	32.5	37.5	28.0	39.5
76-77	33.05965655132759	34.0	31.0	36.0	26.0	39.0
78-79	33.40777143772965	35.0	32.0	36.0	27.0	39.0
80-81	33.28168468166331	35.0	32.0	36.0	27.0	38.5
82-83	32.8417971256124	34.0	31.5	36.0	26.5	37.0
84-85	32.587059363456305	34.0	31.0	35.0	26.0	37.0
86-87	32.48492799774915	34.0	31.0	35.0	26.0	36.5
88-89	32.315137480074206	34.0	31.0	35.0	26.0	36.0
90-91	32.173133077879385	34.0	31.0	35.0	26.0	36.0
92-93	32.07591201812739	34.0	31.0	35.0	26.0	35.0
94-95	32.01495390406261	34.0	31.0	35.0	26.0	35.0
96-97	31.916470331303422	34.0	31.0	35.0	26.0	35.0
98-99	32.1213236412757	34.0	31.0	35.0	26.5	35.0
100-101	31.231243637042063	33.0	29.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	7.0
19	27.0
20	148.0
21	768.0
22	3431.0
23	11103.0
24	28582.0
25	58782.0
26	105161.0
27	167116.0
28	241564.0
29	325825.0
30	420580.0
31	529709.0
32	667885.0
33	846124.0
34	1099579.0
35	1455132.0
36	2025168.0
37	2740166.0
38	2585512.0
39	1268325.0
40	40496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.77673431505917	21.944574962776628	14.381811603569888	24.896879118594313
2	14.915001733123114	22.689700378989023	39.32266894941004	23.072628938477823
3	17.714156860178313	26.47237190797163	31.25254468053791	24.56092655131215
4	12.588490580348836	18.457390845573503	38.296988021972766	30.657130552104896
5	12.697591646097205	38.156887366896946	36.22662040504227	12.918900581963575
6	30.557122915439848	38.36737639002023	18.057004935986743	13.01849575855317
7	26.210732505356948	32.61987567359429	23.488341236246846	17.681050584801923
8	24.49320472547036	37.13904271813717	21.175109549906676	17.192643006485792
9	25.386634056461887	15.935898514416404	21.22783439651629	37.44963303260542
10-11	24.231847749738563	26.80270894503115	29.26764168990349	19.697801615326796
12-13	24.563544417383262	24.355394465156394	29.417119947145203	21.663941170315137
14-15	21.75805457695304	25.942053964143824	26.578907052025176	25.72098440687796
16-17	21.644039555279424	28.93826846890176	27.48462549234127	21.93306648347755
18-19	21.70915711286793	28.211966467621547	28.917788166355272	21.161088253155246
20-21	22.334099326235172	27.47223881332031	28.776558462688325	21.417103397756193
22-23	22.05838084311482	27.47071471450641	28.640361223809425	21.83054321856935
24-25	21.908837389559274	27.5409206005708	28.755771590798407	21.794470419071523
26-27	21.909889918516374	27.660200979074055	28.64184553105102	21.788063571358553
28-29	21.81580646096043	27.73150993933049	28.72501826154728	21.727665338161795
30-31	22.060531388821346	27.640240260927172	28.511752865692436	21.787475484559046
32-33	21.900838690327777	27.770387336552087	28.502139098256045	21.826634874864084
34-35	21.96185677873509	27.808139120538566	28.572038150206225	21.657965950520115
36-37	21.98996458704032	27.729903928853727	28.478120222993702	21.802011261112252
38-39	21.81434392176229	27.808873179977244	28.57667384043838	21.80010905782208
40-41	22.026144157305254	27.696359744013314	28.443930309121836	21.833565789559596
42-43	21.94063758946151	27.823994567871697	28.265609587580233	21.96975825508656
44-45	22.05583828760765	27.884499155046637	28.085586221651848	21.97407633569387
46-47	22.127010808856365	27.827968396753494	27.890283641129137	22.154737153261003
48-49	22.046966010530202	27.93623785947731	27.878843617056297	22.13795251293619
50-51	22.02296937754079	28.01695579904872	27.834842550377935	22.125232273032555
52-53	22.169345343721737	27.930337277649546	27.782933438200104	22.11738394042861
54-55	22.126443721254805	27.840442138986198	27.791657655653808	22.241456484105193
56-57	22.118913638198283	27.972669928720194	27.780876781926295	22.127539651155228
58-59	22.10036024428299	28.035357180712595	27.755560792155247	22.108721782849166
60-61	22.19756276169387	28.025325754455814	27.7064017694897	22.07070971436062
62-63	22.144219236993205	28.122638280274824	27.658174006550546	22.07496847618142
64-65	22.127105889320465	28.027781918333535	27.684964737536248	22.160147454809753
66-67	22.17694960658782	28.01601324935391	27.582564558926798	22.22447258513147
68-69	22.133940245491484	27.993091335161825	27.662280061761884	22.210688357584804
70-71	22.08880230289244	27.764100670439074	27.779082328965167	22.368014697703327
72-73	22.169946858873264	27.836991718064684	27.687122019017224	22.305939404044825
74-75	22.0270879653794	27.932146859328988	27.672870019591013	22.367895155700598
76-77	22.172928077713294	27.89684914572551	27.60951517478358	22.320707601777613
78-79	22.132537167656025	27.879160202927096	27.60725850701012	22.381044122406767
80-81	22.098249078896945	27.879886534839248	27.67671997845138	22.345144407812427
82-83	22.039521032261995	27.88295301190547	27.69622886632487	22.381297089507665
84-85	22.17254188222575	27.815857041285945	27.70620807676851	22.305392999719793
86-87	22.037123814442513	27.797708351562516	27.776371949933694	22.388795884061278
88-89	22.067165766280148	27.762132597840004	27.78259474598596	22.388106889893884
90-91	22.077489671124777	27.7510447406574	27.866342450125604	22.30512313809222
92-93	22.05289297809449	27.665096515131232	27.913250424015406	22.368760082758868
94-95	21.863006499692446	27.702153646657525	28.031430902846665	22.40340895080336
96-97	21.775723898734135	27.767027518944776	28.070703316735475	22.386545265585617
98-99	21.85377382318935	28.406264781712075	28.202765881460444	21.53719551363813
100-101	24.55548873715067	36.18660084595356	29.468053271127665	9.789857145768103
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3158.0
1	2843.5
2	3764.5
3	5343.0
4	7069.0
5	10671.0
6	13746.5
7	16378.0
8	18292.0
9	18507.0
10	17584.0
11	16380.5
12	17852.5
13	23481.5
14	36732.0
15	54420.0
16	67342.0
17	72970.0
18	74470.0
19	74521.0
20	73664.0
21	72978.5
22	74939.0
23	83823.5
24	99772.5
25	122181.5
26	151060.5
27	181320.5
28	213361.5
29	247628.5
30	279368.5
31	311281.5
32	344598.0
33	377511.5
34	411309.5
35	443992.5
36	477548.5
37	508144.0
38	533491.5
39	555672.0
40	576764.5
41	596895.0
42	611389.5
43	625574.5
44	638193.5
45	647150.5
46	653644.5
47	654714.5
48	649317.0
49	636685.0
50	621630.0
51	603393.5
52	576653.5
53	546239.0
54	514715.5
55	479083.5
56	441647.5
57	401855.5
58	358134.0
59	312151.5
60	265522.5
61	219581.0
62	177172.0
63	139560.5
64	105958.0
65	79889.5
66	60312.0
67	46088.5
68	35344.5
69	25866.5
70	18792.0
71	13732.5
72	9903.5
73	7058.0
74	4877.0
75	3312.5
76	2291.5
77	1584.5
78	1110.5
79	780.0
80	541.0
81	375.5
82	275.0
83	193.0
84	139.5
85	103.0
86	80.5
87	65.0
88	47.0
89	33.0
90	21.5
91	12.0
92	7.5
93	4.5
94	2.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0012721262769993414
3	2.051816575805389E-5
4	6.839388586017964E-6
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0016004169291282037
18-19	0.0014841473231658982
20-21	2.3939053631756207E-5
22-23	0.0026507157650824185
24-25	1.676380435997129E-4
26-27	0.001211642861078985
28-29	5.172051725860571E-4
30-31	1.5094847099490826E-4
32-33	9.635872425867017E-4
34-35	0.01707220647896805
36-37	0.03223037502072487
38-39	0.0075367765709058
40-41	0.004595211378126602
42-43	0.007945606158676623
44-45	0.0056334310448076285
46-47	0.0032213211849396206
48-49	0.001451911045254649
50-51	1.8525852543665718E-4
52-53	1.3011381744449727E-4
54-55	0.004841388139552606
56-57	0.01265434000029798
58-59	0.010287304182221943
60-61	0.008516868682554971
62-63	0.0020327598760303935
64-65	0.0014834938597314052
66-67	0.0014820276204217308
68-69	4.3056442518271425E-6
70-71	1.751728255096478E-5
72-73	7.704522220532374E-4
74-75	8.422860185955017E-4
76-77	1.0137339757457696E-4
78-79	8.586443788235774E-4
80-81	0.002363402963458537
82-83	0.004563443876375671
84-85	0.012005336700667685
86-87	0.007880483700126407
88-89	0.07140789005741523
90-91	0.0
92-93	0.0
94-95	1.244240157805848E-4
96-97	0.007995177167520557
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	473.0
20-21	1458.0
22-23	2868.0
24-25	6190.0
26-27	9037.0
28-29	17402.0
30-31	41380.0
32-33	60704.0
34-35	87896.0
36-37	128280.0
38-39	140023.0
40-41	142885.0
42-43	161611.0
44-45	190881.0
46-47	189470.0
48-49	174999.0
50-51	160473.0
52-53	162464.0
54-55	177677.0
56-57	184312.0
58-59	183253.0
60-61	179036.0
62-63	174506.0
64-65	186205.0
66-67	196611.0
68-69	195111.0
70-71	192819.0
72-73	185003.0
74-75	190063.0
76-77	191260.0
78-79	203686.0
80-81	208251.0
82-83	199652.0
84-85	202071.0
86-87	214455.0
88-89	231594.0
90-91	253105.0
92-93	274733.0
94-95	349017.0
96-97	431177.0
98-99	1194236.0
100-101	6944863.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.50194930469254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.90911821931712	78.63815935541672
2	6.411603997260978	11.092324878656665
3	1.5143073667441087	3.929716172094641
4	0.5436163837067446	1.8809550745840447
5	0.2259875929736615	0.9774183655448583
6	0.13849719877592268	0.7188166600414075
7	0.07125873070677591	0.43148133777799874
8	0.045944409186913254	0.31794247634563366
9	0.027826751135781043	0.21663613944554652
>10	0.10821391680217231	1.5170536654079314
>50	0.0028925652620460274	0.1697323937146565
>100	7.065415383143843E-4	0.09128458004862157
>500	2.6326589489226623E-5	0.01847890092118231
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.367877717203593E-5	0.0
2	0.0	1.367877717203593E-5	0.0	1.367877717203593E-5	0.0
3	0.0	1.367877717203593E-5	0.0	4.103633151610778E-5	0.0
4	0.0	4.103633151610778E-5	0.0	4.787572010212575E-5	0.0
5	0.0	4.787572010212575E-5	0.0	4.787572010212575E-5	0.0
6	8.207266303221557E-5	4.787572010212575E-5	0.0	5.471510868814372E-5	6.839388586017965E-6
7	8.207266303221557E-5	4.787572010212575E-5	0.0	6.839388586017964E-5	6.839388586017965E-6
8	8.207266303221557E-5	7.523327444619761E-5	0.0	7.523327444619761E-5	1.367877717203593E-5
9	8.207266303221557E-5	1.8466349182248503E-4	0.0	1.0943021737628744E-4	1.367877717203593E-5
10-11	8.549235732522456E-5	1.8466349182248503E-4	0.0	1.6072563177142217E-4	1.367877717203593E-5
12-13	9.233174591124252E-5	1.8466349182248503E-4	0.0	1.7782410323646707E-4	1.367877717203593E-5
14-15	1.0601052308327846E-4	2.3595890621761975E-4	0.0	2.0518165758053893E-4	2.3937860051062874E-5
16-17	1.4362716030637726E-4	2.462179890966467E-4	0.0	2.1886043475257484E-4	3.419694293008982E-5
18-19	1.5046654889239522E-4	2.769952377337276E-4	0.0	2.4279829480363773E-4	4.103633151610778E-5
20-21	1.5730593747841318E-4	3.077724863708084E-4	0.0	2.735755434407186E-4	4.103633151610778E-5
22-23	1.6756502035744013E-4	3.5564820647293415E-4	0.0	3.2829065212886227E-4	4.103633151610778E-5
24-25	1.7782410323646707E-4	3.9668453798904195E-4	0.0	3.966845379890419E-4	4.103633151610778E-5
26-27	1.8466349182248503E-4	4.1720270374709584E-4	0.0	5.916071126905539E-4	4.103633151610778E-5
28-29	1.91502880408503E-4	4.240420923331138E-4	0.0	0.001131918810985973	4.103633151610778E-5
30-31	1.91502880408503E-4	4.7191781243523955E-4	0.0	0.0022569982333859283	4.103633151610778E-5
32-33	1.9834226899452095E-4	4.7191781243523955E-4	0.0	0.004777312927333548	4.787572010212575E-5
34-35	2.1202104616655691E-4	5.539904754674551E-4	0.0	0.008617629618382635	5.471510868814372E-5
36-37	2.1886043475257487E-4	6.360631384996707E-4	0.0	0.014540540133874192	5.471510868814372E-5
38-39	2.1886043475257487E-4	6.497419156717067E-4	0.0	0.02278200338002584	5.471510868814372E-5
40-41	2.2569982333859283E-4	7.249751901179043E-4	0.0	0.031221808895172006	6.839388586017964E-5
42-43	2.2569982333859283E-4	7.283948844109133E-4	0.0	0.039610318995923044	6.839388586017964E-5
44-45	2.2911951763160182E-4	7.933690759780838E-4	0.0	0.049253856902208376	6.839388586017964E-5
46-47	2.3253921192461079E-4	8.549235732522455E-4	0.0	0.06056620562348208	6.839388586017964E-5
48-49	2.3253921192461079E-4	8.549235732522455E-4	0.0	0.07091762024842027	6.839388586017964E-5
50-51	2.4963768338965566E-4	9.50675013456497E-4	0.0	0.08089970788971348	6.839388586017964E-5
52-53	2.5989676626868264E-4	9.64353790628533E-4	0.0	0.09018417789523288	7.181358015318862E-5
54-55	2.701558491477096E-4	9.78032567800569E-4	0.0	0.10099725124972728	7.523327444619761E-5
56-57	2.7357554344071855E-4	9.917113449726049E-4	0.0	0.11199498809604416	7.865296873920659E-5
58-59	2.7357554344071855E-4	9.917113449726049E-4	0.0	0.12253790560139086	8.207266303221557E-5
60-61	2.8383462631974553E-4	0.0010122295107306586	0.0	0.1328995793092081	8.207266303221557E-5
62-63	3.077724863708084E-4	0.0010224885936096855	0.0	0.14216011145467639	8.549235732522456E-5
64-65	3.077724863708084E-4	0.0010943021737628742	0.0	0.15162924495201827	8.891205161823354E-5
66-67	3.077724863708084E-4	0.0010943021737628742	0.0	0.1613480161327498	8.891205161823354E-5
68-69	3.2145126354284434E-4	0.0011079809509349102	0.0	0.17202088202123084	8.891205161823354E-5
70-71	3.3854973500788924E-4	0.001125079422399955	0.0	0.18205084538262617	8.891205161823354E-5
72-73	3.7616637223098805E-4	0.001138758199571991	0.0	0.19038806006898207	9.57514402042515E-5
74-75	3.7616637223098805E-4	0.001169535448209072	0.0	0.19815076611411248	9.57514402042515E-5
76-77	3.7616637223098805E-4	0.0012071520854321709	0.0	0.2055031088440818	9.57514402042515E-5
78-79	3.7958606652399704E-4	0.0012310899454832337	0.0	0.21193897350352467	9.57514402042515E-5
80-81	3.83005760817006E-4	0.0012345096397762426	0.0	0.2159776324635683	9.57514402042515E-5
82-83	3.83005760817006E-4	0.0012413490283622606	0.0	0.21889463169550494	9.57514402042515E-5
84-85	3.83005760817006E-4	0.0012584474998273054	0.0	0.2218116309274416	9.57514402042515E-5
86-87	3.8642545511001497E-4	0.0012618671941203143	0.0	0.22213650188527745	9.57514402042515E-5
88-89	3.8984514940302396E-4	0.0012858050541713773	0.0	0.22221173515972367	9.57514402042515E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15445	0.0	43.004795	1
GTATCAA	24980	0.0	29.971718	1
TCAACGC	30150	0.0	23.911257	4
ATCAACG	30465	0.0	23.73005	3
CAACGCA	30730	0.0	23.420683	5
TATCAAC	32160	0.0	22.72985	2
AACGCAG	31850	0.0	22.559204	6
GTACATG	36030	0.0	19.852957	1
TACATGG	36010	0.0	19.271862	2
ACGCAGA	38060	0.0	18.814947	7
CGCAGAG	38590	0.0	18.55654	8
ACATGGG	36530	0.0	18.413584	3
GTGGTAT	7700	0.0	16.144531	1
CATGGGG	26960	0.0	16.056252	4
AGAGTAC	41205	0.0	15.855978	10-11
GAGTACT	26830	0.0	15.542378	12-13
GCAGAGT	46420	0.0	15.235807	9
CAGAGTA	45065	0.0	15.011167	10-11
TGGTATC	7755	0.0	14.733105	2
TATACTG	7575	0.0	13.91459	5
>>END_MODULE