FastQCFastQC Report
Fri 27 May 2016
SRR2139920_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139920_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12464832
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA168150.039.897181
CTTATAC126150.029.2555771
GTATCAA258750.028.4953691
TATACAC155850.026.6338213
ATCAACG318050.022.4774783
CAACGCA321750.022.1941155
TCAACGC325500.021.9999084
TATCAAC338550.021.5067562
AACGCAG332350.021.4621666
TTATACA201000.019.9742222
TACATGG347300.019.6512
ACACATC195050.019.2289316
ACATGGG354250.018.8585383
ACGCAGA377300.018.8415977
GTACATG376700.018.7442461
ACATCTC203600.018.735998
TACACAT222350.018.6502295
CGCAGAG383600.018.5217198
TATGCCG113800.017.43277742-43
CATGGGG256250.017.0576934
Produced by FastQC (version 0.11.5)