##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139920_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12464832
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81918713384986	31.0	31.0	34.0	30.0	34.0
2	32.003764832129306	31.0	31.0	34.0	30.0	34.0
3	32.03244905346498	31.0	31.0	34.0	30.0	34.0
4	35.75841391203668	37.0	35.0	37.0	35.0	37.0
5	35.5845997763949	37.0	35.0	37.0	33.0	37.0
6	35.58824065980191	37.0	35.0	37.0	33.0	37.0
7	35.4756862346801	37.0	35.0	37.0	33.0	37.0
8	35.44594255261523	37.0	35.0	37.0	33.0	37.0
9	37.02350781783501	39.0	37.0	39.0	33.0	39.0
10-11	36.865913555834524	39.0	37.0	39.0	33.0	39.0
12-13	36.82400998264558	39.0	37.0	39.0	32.5	39.0
14-15	37.96001313936682	40.0	37.0	41.0	33.0	41.0
16-17	37.96941944343895	40.0	37.0	41.0	33.0	41.0
18-19	37.950222273352736	40.0	37.0	41.0	33.0	41.0
20-21	37.913703665603514	40.0	37.0	41.0	33.0	41.0
22-23	37.832439872047935	40.0	37.0	41.0	32.0	41.0
24-25	37.73131082306482	39.0	37.0	41.0	32.0	41.0
26-27	37.5686325635172	39.0	36.5	41.0	32.0	41.0
28-29	37.42475539722746	39.0	36.0	41.0	32.0	41.0
30-31	37.28373922091539	39.0	36.0	40.0	31.0	41.0
32-33	37.45906810813222	39.0	36.0	40.5	32.0	41.0
34-35	37.44215642629531	39.0	36.0	41.0	32.0	41.0
36-37	37.33306422792148	39.0	36.0	40.5	31.5	41.0
38-39	37.16203506487625	39.0	36.0	40.0	31.0	41.0
40-41	37.15170004500401	39.0	36.0	40.0	31.0	41.0
42-43	37.030481074072426	39.0	35.5	40.0	31.0	41.0
44-45	37.079303091063665	39.0	35.5	40.0	31.0	41.0
46-47	37.22627800433824	39.0	36.0	41.0	31.0	41.0
48-49	37.15835449060717	39.0	36.0	41.0	31.0	41.0
50-51	37.028139312055565	39.0	35.5	40.0	31.0	41.0
52-53	36.89258751511835	39.0	35.0	40.0	31.0	41.0
54-55	36.75741828861973	39.0	35.0	40.0	30.5	41.0
56-57	36.583354535855236	38.0	35.0	40.0	30.0	41.0
58-59	36.43405014040462	38.0	35.0	40.0	30.0	41.0
60-61	36.22891513265601	38.0	34.0	40.0	30.0	41.0
62-63	35.97230231451771	38.0	34.0	40.0	29.0	41.0
64-65	35.741948039721024	37.0	34.0	40.0	29.0	41.0
66-67	35.466218333765696	37.0	34.0	39.5	29.0	41.0
68-69	35.13736339717521	36.0	34.0	39.0	28.5	40.5
70-71	34.792975127538426	36.0	33.0	39.0	28.0	40.0
72-73	34.50518808412379	35.0	33.0	38.5	28.0	40.0
74-75	34.147551797078	35.0	33.0	37.5	28.0	39.5
76-77	33.07651793557992	34.0	31.0	36.0	26.0	39.0
78-79	33.4305819095313	35.0	32.0	36.0	27.0	39.0
80-81	33.312240793764516	35.0	32.0	36.0	27.0	38.5
82-83	32.8773687005967	34.0	31.5	36.0	26.5	37.0
84-85	32.62928486061944	34.0	31.0	35.0	26.0	37.0
86-87	32.53068276908793	34.0	31.0	35.0	26.0	36.5
88-89	32.36145460219423	34.0	31.0	35.0	26.0	36.0
90-91	32.221225123603645	34.0	31.0	35.0	26.0	36.0
92-93	32.12507386206531	34.0	31.0	35.0	26.0	35.5
94-95	32.06373434501705	34.0	31.0	35.0	26.0	35.0
96-97	31.96667829613756	34.0	31.0	35.0	26.0	35.0
98-99	32.16747107328592	34.0	31.0	35.0	26.5	35.0
100-101	31.277553397993824	33.0	29.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	1.0
18	2.0
19	13.0
20	129.0
21	685.0
22	2966.0
23	9326.0
24	24347.0
25	50933.0
26	91068.0
27	144238.0
28	207626.0
29	278801.0
30	360040.0
31	454560.0
32	568426.0
33	720488.0
34	937540.0
35	1238689.0
36	1724382.0
37	2335098.0
38	2195838.0
39	1083765.0
40	35870.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.453466520848416	22.284295528411455	14.387349945831602	24.87488800490853
2	14.676354422247396	22.31551435501416	40.11509651800622	22.893034704732234
3	17.339836751872006	27.046283587203646	31.58240678632655	24.0314728745978
4	12.188438365259563	18.58223646710276	38.67367133556345	30.55565383207423
5	12.256771691748433	38.64684257276792	36.52175977983498	12.57462595564866
6	29.958703013406033	38.9206930346113	18.101800329117953	13.018803622864713
7	25.670927614587985	32.932694159054854	23.682316777313968	17.714061449043196
8	24.471970420459737	37.19402716378368	21.15231075717667	17.181691658579915
9	25.276257233150034	15.811877769391517	21.279099469611783	37.632765527846665
10-11	24.167409556743323	26.97003056278657	29.212230858787347	19.650329021682765
12-13	24.571943689253093	24.35938567001946	29.262889383507133	21.805781257220314
14-15	21.77539576947367	25.885824213274596	26.58102812777581	25.757751889475927
16-17	21.595235325500514	28.85352978222413	27.46779091270112	22.083443979574234
18-19	21.534975375386217	28.17680382441161	28.955320691595137	21.33290010860703
20-21	22.40532574915862	27.51274560391945	28.59855139513773	21.4833772517842
22-23	21.95214044095707	27.52810320878677	28.567407278960065	21.952349071296087
24-25	21.81368271417748	27.577692932766173	28.865803511960657	21.742820841095682
26-27	21.88107789875874	27.75043612725104	28.609558232764044	21.758927741226188
28-29	21.686793168761522	27.862619557481327	28.680504545890294	21.770082727866853
30-31	21.964740449277308	27.786194079497307	28.628455216952904	21.620610254272478
32-33	21.631674653964406	27.7832471899238	28.75856789143859	21.826510264673203
34-35	21.69248240002337	27.830074619356893	28.696453551965778	21.780989428653964
36-37	21.822496227120887	27.723730658823246	28.81784619319715	21.635926920858715
38-39	21.683600904185983	27.775294107941328	28.816588842872935	21.724516144999754
40-41	21.89955578377198	27.716229271923847	28.73813499205179	21.646079952252382
42-43	21.98422932942225	27.80651943885097	28.501139897438517	21.708111334288265
44-45	21.794379285571644	28.187881022985884	28.274012314457188	21.74372737698528
46-47	22.131597573726737	27.77803906039422	27.827594700946022	22.26276866493302
48-49	22.07271722324034	27.777915667799892	28.00810219317033	22.141264915789442
50-51	21.81467243355302	27.96881998783575	27.978958345021166	22.237549233590066
52-53	21.91580075525686	28.032226072387456	28.013048395654096	22.03892477670159
54-55	21.98153847840947	27.895663434087925	27.857089984610827	22.265708102891775
56-57	22.14413050934759	28.06646247524622	27.61731710841015	22.172089906996042
58-59	21.924707987873504	28.20278734522731	27.9796222275359	21.892882439363284
60-61	22.127088775826387	28.409572029675513	27.546531550689217	21.916807643808887
62-63	22.04216691595569	28.37919351902023	27.52598573692436	22.05265382809972
64-65	21.906187730741568	28.297966646214757	27.623557497024215	22.172288126019456
66-67	22.13213168684979	28.291727664215877	27.391357343644167	22.184783305290168
68-69	22.106872722121498	28.063340350788877	27.624162331851824	22.2056245952378
70-71	22.032810921892406	27.71742986334517	27.799137821498327	22.450621393264093
72-73	22.17799707099191	27.843107459440226	27.75395590815664	22.22493956141122
74-75	21.956531337021858	28.02570085910024	27.727293285062338	22.290474518815564
76-77	22.00093541792683	28.055991632293324	27.587734872014387	22.355338077765463
78-79	22.054820432434354	28.039253125842134	27.58165104575357	22.324275395969938
80-81	21.98294573846268	27.87014922834396	27.737571467832357	22.409333565361006
82-83	22.044606585993595	27.81648363532571	27.821963517106546	22.31694626157415
84-85	22.300285895788054	27.79056845839473	27.64370718445636	22.26543846136085
86-87	21.848888939379346	27.92475603340475	27.782993334494343	22.443361692721556
88-89	22.138950253852173	27.750772110383114	27.784127748818378	22.326149886946332
90-91	22.09812868562808	27.74757646615262	27.940859933749117	22.21343491447018
92-93	22.116214222625743	27.72960794593946	27.839089802799162	22.31508802863564
94-95	21.78452866401267	27.824622261885885	27.990743702572736	22.40010537152872
96-97	21.673395856915633	27.831922459993486	28.130685331794915	22.363996351295967
98-99	21.674291459793977	28.510707638899667	28.24403884331953	21.57096205798683
100-101	24.547888457277452	36.32898911565223	29.38352016474729	9.739602262323034
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2614.0
1	2411.5
2	3572.0
3	5241.0
4	7349.5
5	11814.5
6	15282.5
7	17542.0
8	18442.5
9	17385.0
10	15761.5
11	14458.0
12	16357.0
13	22875.0
14	36981.5
15	55296.5
16	68551.0
17	73814.5
18	74117.5
19	72215.5
20	68954.5
21	66530.5
22	67601.0
23	75778.0
24	90749.5
25	111920.0
26	141757.5
27	172248.0
28	197116.0
29	221048.0
30	241293.0
31	261037.0
32	287305.0
33	315543.0
34	345762.0
35	378605.5
36	414028.5
37	442813.5
38	457219.5
39	467629.5
40	483521.5
41	494985.0
42	501659.0
43	516110.5
44	531151.0
45	543504.5
46	552087.0
47	559696.0
48	574628.0
49	573363.5
50	551791.5
51	534182.0
52	514644.5
53	484088.0
54	446594.0
55	409298.0
56	376628.0
57	341111.5
58	301450.5
59	262656.0
60	222171.0
61	178877.5
62	139335.0
63	107150.0
64	79503.5
65	57867.0
66	42045.0
67	31379.0
68	23807.0
69	17577.5
70	12844.0
71	9168.0
72	6414.5
73	4337.0
74	2912.0
75	1966.0
76	1397.5
77	944.5
78	648.5
79	467.5
80	329.5
81	241.0
82	171.5
83	108.5
84	70.0
85	47.0
86	37.0
87	29.0
88	22.5
89	18.5
90	13.5
91	11.0
92	8.5
93	5.0
94	2.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0013076790766213295
3	2.4067713066650236E-5
4	2.4067713066650236E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0014601079260434476
18-19	0.0013598257882657383
20-21	1.6045953685841808E-5
22-23	0.0025115252328246074
24-25	1.7657248330537267E-4
26-27	0.001260629940796161
28-29	4.8615473501654034E-4
30-31	1.3683570491074368E-4
32-33	0.0010416964859015063
34-35	0.016161128397244432
36-37	0.032885296526853576
38-39	0.007543938907046175
40-41	0.004484473884524631
42-43	0.007099757192517521
44-45	0.005035915251350426
46-47	0.0029014520511663707
48-49	0.001256369761745289
50-51	1.4693249694013076E-4
52-53	9.920255574642528E-5
54-55	0.004326874684708135
56-57	0.011571105720021908
58-59	0.009279235172465183
60-61	0.007480250917192186
62-63	0.0016870176745973353
64-65	0.001249722640607628
66-67	0.0013269539686202529
68-69	0.0
70-71	5.205322921577021E-6
72-73	7.094799332072294E-4
74-75	8.076209264273489E-4
76-77	1.0408129603782774E-4
78-79	8.423011444139259E-4
80-81	0.002713489627301915
82-83	0.004881732626168989
84-85	0.013384217432620587
86-87	0.008694747943244987
88-89	0.06912417483325214
90-91	0.0
92-93	1.3024249655069027E-5
94-95	1.54562590221712E-4
96-97	0.008128750437270713
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	404.0
20-21	1343.0
22-23	2674.0
24-25	4717.0
26-27	7951.0
28-29	15721.0
30-31	36581.0
32-33	52699.0
34-35	75696.0
36-37	112741.0
38-39	126795.0
40-41	127102.0
42-43	143420.0
44-45	167738.0
46-47	167598.0
48-49	155847.0
50-51	142548.0
52-53	141385.0
54-55	158487.0
56-57	167052.0
58-59	166323.0
60-61	166136.0
62-63	160650.0
64-65	168582.0
66-67	179596.0
68-69	167739.0
70-71	164595.0
72-73	157242.0
74-75	158530.0
76-77	160665.0
78-79	172661.0
80-81	177604.0
82-83	167871.0
84-85	168785.0
86-87	179166.0
88-89	195006.0
90-91	213011.0
92-93	226871.0
94-95	290528.0
96-97	354711.0
98-99	997700.0
100-101	5862361.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.59755332480555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.42107396230038	74.75382998924786
2	6.936695088619188	11.597814751375186
3	1.9085222904271477	4.786433818366904
4	0.7773458835444972	2.5993685580571673
5	0.36853756234559154	1.540441926018972
6	0.20442482962668257	1.025364935737853
7	0.11336571660675396	0.6633967577466535
8	0.07093134324073627	0.4743749399174028
9	0.050528832087973034	0.3801678061423024
>10	0.14542391256769635	1.785677424464631
>50	0.0022441537818599093	0.12387737524949445
>100	7.935746851623336E-4	0.12923890609372674
>500	4.8375222673441884E-5	0.023819021028706568
>1k	6.447494357265025E-5	0.1161937905531604
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.022571022216745E-6	0.0
5	0.0	0.0	0.0	8.022571022216745E-6	0.0
6	0.0	0.0	0.0	8.022571022216745E-6	0.0
7	0.0	0.0	0.0	2.4067713066650236E-5	0.0
8	0.0	0.0	0.0	2.4067713066650236E-5	0.0
9	0.0	2.4067713066650236E-5	0.0	3.209028408886698E-5	0.0
10-11	0.0	2.4067713066650236E-5	0.0	3.209028408886698E-5	0.0
12-13	0.0	2.4067713066650236E-5	0.0	4.41241406221921E-5	0.0
14-15	8.022571022216745E-6	2.8078998577758606E-5	0.0	6.418056817773396E-5	0.0
16-17	8.022571022216745E-6	3.209028408886698E-5	0.0	7.22031391999507E-5	0.0
18-19	8.022571022216745E-6	4.41241406221921E-5	0.0	1.0028213777770931E-4	0.0
20-21	8.022571022216745E-6	5.615799715551722E-5	0.0	1.2033856533325119E-4	0.0
22-23	1.2033856533325117E-5	5.615799715551722E-5	0.0	1.2033856533325119E-4	0.0
24-25	1.604514204443349E-5	5.615799715551722E-5	0.0	1.9655299004431027E-4	0.0
26-27	1.604514204443349E-5	5.615799715551722E-5	0.0	3.409592684442117E-4	0.0
28-29	1.604514204443349E-5	5.615799715551722E-5	0.0	9.546859516437927E-4	0.0
30-31	1.604514204443349E-5	5.615799715551722E-5	0.0	0.0025030421589316244	0.0
32-33	1.604514204443349E-5	6.418056817773396E-5	0.0	0.005980826697062584	0.0
34-35	2.4067713066650236E-5	9.225956675549258E-5	0.0	0.010990922300436943	0.0
36-37	2.4067713066650236E-5	1.0429342328881769E-4	0.0	0.01841982306700965	0.0
38-39	2.4067713066650236E-5	1.0429342328881769E-4	0.0	0.028367811134558413	0.0
40-41	2.4067713066650236E-5	1.0429342328881769E-4	0.0	0.03921432715659545	0.0
42-43	2.4067713066650236E-5	1.0429342328881769E-4	0.0	0.04890158166592217	0.0
44-45	2.4067713066650236E-5	1.0429342328881769E-4	0.0	0.06076295292226963	0.0
46-47	2.4067713066650236E-5	1.0429342328881769E-4	0.0	0.07330624271550551	0.0
48-49	2.4067713066650236E-5	1.0429342328881769E-4	0.0	0.0853240541067862	0.0
50-51	2.4067713066650236E-5	1.0429342328881769E-4	0.0	0.0964433375435786	0.0
52-53	3.209028408886698E-5	1.0429342328881769E-4	0.0	0.10728985356561564	0.0
54-55	3.209028408886698E-5	1.0429342328881769E-4	0.0	0.11836902414729697	0.0
56-57	3.209028408886698E-5	1.0429342328881769E-4	0.0	0.12970491700168923	0.0
58-59	3.209028408886698E-5	1.0429342328881769E-4	0.0	0.14060759102088177	0.0
60-61	3.209028408886698E-5	1.0429342328881769E-4	0.0	0.15049942109127504	0.0
62-63	3.209028408886698E-5	1.0429342328881769E-4	0.0	0.15956893763189106	0.0
64-65	3.209028408886698E-5	1.0429342328881769E-4	0.0	0.16914387614690674	0.0
66-67	3.209028408886698E-5	1.0429342328881769E-4	0.0	0.17853028424290035	0.0
68-69	3.209028408886698E-5	1.0429342328881769E-4	0.0	0.18821753875222708	0.0
70-71	3.209028408886698E-5	1.0429342328881769E-4	0.0	0.19735925843204305	0.0
72-73	3.209028408886698E-5	1.0429342328881769E-4	0.0	0.2053016037440376	0.0
74-75	3.209028408886698E-5	1.0429342328881769E-4	0.0	0.21289496721656576	0.0
76-77	3.209028408886698E-5	1.0429342328881769E-4	0.0	0.220380025980294	0.0
78-79	3.209028408886698E-5	1.0830470879992607E-4	0.0	0.226930455219934	0.0
80-81	4.0112855111083726E-5	1.1231599431103444E-4	0.0	0.23097784230064233	0.0
82-83	4.0112855111083726E-5	1.1231599431103444E-4	0.0	0.2341788481385068	0.0
84-85	4.0112855111083726E-5	1.1231599431103444E-4	0.0	0.23717527841530475	0.0
86-87	4.0112855111083726E-5	1.1231599431103444E-4	0.0	0.2375362941113045	0.0
88-89	4.0112855111083726E-5	1.1231599431103444E-4	0.0	0.23763657624908222	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16815	0.0	39.89718	1
CTTATAC	12615	0.0	29.255577	1
GTATCAA	25875	0.0	28.495369	1
TATACAC	15585	0.0	26.633821	3
ATCAACG	31805	0.0	22.477478	3
CAACGCA	32175	0.0	22.194115	5
TCAACGC	32550	0.0	21.999908	4
TATCAAC	33855	0.0	21.506756	2
AACGCAG	33235	0.0	21.462166	6
TTATACA	20100	0.0	19.974222	2
TACATGG	34730	0.0	19.651	2
ACACATC	19505	0.0	19.228931	6
ACATGGG	35425	0.0	18.858538	3
ACGCAGA	37730	0.0	18.841597	7
GTACATG	37670	0.0	18.744246	1
ACATCTC	20360	0.0	18.73599	8
TACACAT	22235	0.0	18.650229	5
CGCAGAG	38360	0.0	18.521719	8
TATGCCG	11380	0.0	17.432777	42-43
CATGGGG	25625	0.0	17.057693	4
>>END_MODULE