FastQCFastQC Report
Fri 27 May 2016
SRR2139919_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139919_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32688308
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA420250.051.5289081
GTATCAA773300.038.5995671
TCAACGC949000.030.79854
ATCAACG949400.030.7673343
CAACGCA967150.030.2160555
AACGCAG998600.029.3322186
TATCAAC1009800.029.2302152
ACGCAGA1141750.025.6170587
CGCAGAG1156650.025.2871748
GTACATG984650.021.7120021
GCAGAGT1368500.021.3464839
TACATGG975750.021.343742
ACATGGG982200.020.5928653
AGAGTAC1219400.019.80552110-11
GAGTACT805200.019.66706312-13
CAGAGTA1325100.018.92742210-11
CATGGGG697450.018.4772934
AGTACTT868100.017.74498612-13
GTACTTT909000.017.34567314-15
GTGGTAT187700.015.6173241
Produced by FastQC (version 0.11.5)