##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139919_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32688308
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.756297725780115	34.0	31.0	34.0	31.0	34.0
2	32.80883540989641	34.0	31.0	34.0	31.0	34.0
3	32.89717237735278	34.0	31.0	34.0	31.0	34.0
4	36.312108323257355	37.0	37.0	37.0	35.0	37.0
5	36.255606530628626	37.0	37.0	37.0	35.0	37.0
6	36.32292671740611	37.0	37.0	37.0	35.0	37.0
7	36.2963913886274	37.0	37.0	37.0	35.0	37.0
8	36.30335415341779	37.0	37.0	37.0	35.0	37.0
9	38.09727218062189	39.0	39.0	39.0	35.0	39.0
10-11	38.07303193851453	39.0	39.0	39.0	35.0	39.0
12-13	38.06322228424916	39.0	39.0	39.0	35.0	39.0
14-15	39.56967162387237	41.0	40.0	41.0	37.0	41.0
16-17	39.58471935898304	41.0	40.0	41.0	37.0	41.0
18-19	39.629909721849174	41.0	40.0	41.0	37.0	41.0
20-21	39.64398998525526	41.0	40.0	41.0	37.0	41.0
22-23	39.602946451868554	41.0	40.0	41.0	37.0	41.0
24-25	39.57847191591368	41.0	40.0	41.0	37.0	41.0
26-27	39.50729776301027	41.0	40.0	41.0	36.0	41.0
28-29	39.45571315658043	41.0	39.0	41.0	36.0	41.0
30-31	39.37598847927454	41.0	39.0	41.0	36.0	41.0
32-33	39.30061086248956	41.0	39.0	41.0	35.5	41.0
34-35	39.2491956393709	41.0	39.0	41.0	35.0	41.0
36-37	39.20300170097978	41.0	39.0	41.0	35.0	41.0
38-39	39.15863250025397	41.0	39.0	41.0	35.0	41.0
40-41	39.123303565357006	41.0	39.0	41.0	35.0	41.0
42-43	39.051253277759145	41.0	39.0	41.0	35.0	41.0
44-45	38.67581317905143	40.5	38.5	41.0	34.5	41.0
46-47	38.73380738539767	40.0	38.0	41.0	34.5	41.0
48-49	38.816462445447	40.0	38.0	41.0	35.0	41.0
50-51	38.7800428925129	40.0	38.0	41.0	35.0	41.0
52-53	38.68727684156478	40.0	38.0	41.0	34.5	41.0
54-55	38.56793738161849	40.0	38.0	41.0	34.0	41.0
56-57	38.412784891282726	40.0	37.5	41.0	34.0	41.0
58-59	38.25930865274017	40.0	37.0	41.0	34.0	41.0
60-61	38.077889079674804	40.0	36.5	41.0	34.0	41.0
62-63	37.85046275995398	39.0	36.0	41.0	33.5	41.0
64-65	37.59942164551653	39.0	35.5	41.0	33.0	41.0
66-67	37.33565716889587	39.0	35.0	41.0	33.0	41.0
68-69	36.971185024879645	38.0	35.0	40.0	32.5	41.0
70-71	36.65805002042893	37.0	35.0	40.0	32.0	41.0
72-73	36.371961520470606	37.0	35.0	39.0	32.0	41.0
74-75	36.03296308737713	36.0	35.0	39.0	32.0	41.0
76-77	35.69487340913591	36.0	35.0	39.0	32.0	40.0
78-79	35.36012298262824	35.0	35.0	37.0	31.5	39.5
80-81	35.050329602931974	35.0	34.0	37.0	31.0	39.0
82-83	34.762854171784944	35.0	34.0	36.5	31.0	39.0
84-85	34.50855437610476	35.0	34.0	36.0	31.0	37.5
86-87	34.28882959973974	35.0	34.0	36.0	31.0	37.0
88-89	34.23793856453892	35.0	34.0	35.5	31.0	37.0
90-91	34.20587126598684	35.0	34.0	35.0	31.0	36.0
92-93	34.128874692752305	35.0	34.0	35.0	31.0	36.0
94-95	34.171702959827726	35.0	34.0	35.0	32.0	36.0
96-97	34.22084421406335	35.0	34.0	35.0	32.0	36.0
98-99	34.322186002260764	35.0	35.0	35.0	32.5	36.0
100	34.107897218216394	35.0	34.0	35.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	7.0
19	19.0
20	97.0
21	495.0
22	2253.0
23	8314.0
24	22491.0
25	49116.0
26	92073.0
27	148992.0
28	218888.0
29	297437.0
30	384921.0
31	488417.0
32	619273.0
33	800726.0
34	1105014.0
35	1623966.0
36	2711332.0
37	6055191.0
38	1.0486759E7
39	7073245.0
40	499282.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.55840063670472	20.328718145949924	13.881835058578131	24.231046158767228
2	14.292789336174879	21.771983426000514	41.377846782403054	22.55738045542155
3	17.295312440154444	27.435867895028398	31.00498502400308	24.263834640814082
4	11.847272731277496	18.88951853977881	40.044581077735806	29.21862765120789
5	12.469866933898457	38.58830618464537	36.13886306244156	12.802963819014623
6	28.310584322688097	39.493335048115675	19.50650367097618	12.689576958220048
7	25.223425452305452	33.254801686278775	24.349443232118347	17.172329629297423
8	24.031173897527996	36.80562538612887	21.81572611194024	17.347474604402898
9	25.676961252749976	16.32754908426245	21.31090032578599	36.68458933720158
10-11	23.57318510825882	26.83557497847128	29.867322738178558	19.723917175091344
12-13	24.791411547620697	24.6191642230457	29.619837096422437	20.96958713291117
14-15	21.978125391983795	25.70028421693492	27.24470649782937	25.07688389325191
16-17	21.38509432791688	28.792952513785664	27.77009902133815	22.051854136959307
18-19	21.46769113898462	28.00228295695207	29.48257982640154	21.047446077661775
20-21	22.403052221153658	27.19282639125443	29.239240874084864	21.164880513507043
22-23	22.072508716154363	27.134796272586232	29.12931910543061	21.663375905828797
24-25	21.779288000695754	27.267496370207667	29.25072964594293	21.70248598315365
26-27	21.802884777629373	27.382173278609567	29.108481498207272	21.706460445553784
28-29	21.77711641968714	27.394166861452717	29.115561582162442	21.7131551366977
30-31	22.063461431065925	27.2883840859723	28.988542062058464	21.65961242090331
32-33	21.879050519714873	27.493346656450147	28.92381436805282	21.703788455782156
34-35	22.002918838729475	27.43710406406948	28.960121110769126	21.59985598643192
36-37	21.976418778310855	27.383677901607467	28.906454849264442	21.733448470817233
38-39	21.84375214184412	27.47879089739896	28.937572435036024	21.739884525720893
40-41	22.08130936607923	27.319076133180666	28.80508870871623	21.794525792023876
42-43	21.980517886857527	27.44247396114712	28.606700842015826	21.970307309979535
44-45	22.227361095127836	27.55326411748658	28.357102226748726	21.862272560636857
46-47	22.21704319340832	27.574205851329314	28.04725986909018	22.161491086172187
48-49	22.13873961912718	27.70914198104381	27.99583885762213	22.15627954220688
50-51	22.125890771886766	27.836236534429727	27.837344703634702	22.2005279900488
52-53	22.293381230491594	27.71273225421455	27.80162932263664	22.192257192657213
54-55	22.232139035561246	27.624082095292803	27.899012263223433	22.24476660592252
56-57	22.246504847167547	27.720725234957445	27.84171766875411	22.191052249120897
58-59	22.228541790419882	27.782725508021333	27.824136897709266	22.16459580384952
60-61	22.29101305502445	27.791111379546823	27.784922713513282	22.13295285191544
62-63	22.233747816071357	27.853115648331755	27.739877306567568	22.17325922902932
64-65	22.226499718727172	27.79605301674655	27.729582689545218	22.247864574981058
66-67	22.2634207966303	27.87375060473294	27.592732528386072	22.270096070250688
68-69	22.272415923911776	27.8121382139342	27.684958308090362	22.230487554063657
70-71	22.282124437946766	27.548078860498933	27.738382166404264	22.431414535150036
72-73	22.28940140366181	27.60042086879996	27.684864708779678	22.425313018758548
74-75	22.155922739031595	27.789821459602525	27.668487205037458	22.385768596328422
76-77	22.32135384087157	27.718329739918108	27.5887632496641	22.371553169546225
78-79	22.30061996600843	27.73542477845744	27.556824822865572	22.407130432668563
80-81	22.2936022392453	27.770400048780242	27.527598582179095	22.408399129795363
82-83	22.332889185638905	27.68924001266913	27.63539767210216	22.342473129589802
84-85	22.399266163518995	27.7062859757098	27.58033233470415	22.314115526067056
86-87	22.258721796936054	27.74774515538871	27.66633116144131	22.327201886233926
88-89	22.327539964574417	27.74096981921587	27.574422024187406	22.357068192022304
90-91	22.368311483389967	27.731865958674888	27.65344693939799	22.24637561853715
92-93	22.401322194363658	27.704578182280287	27.5945332460939	22.299566377262156
94-95	22.244445531400046	27.731273213230622	27.686817291712	22.337463963657328
96-97	22.347816919821696	27.887165052295487	27.68971977932706	22.075298248555757
98-99	23.398233535037715	29.82304787813425	27.91193363108783	18.8667849557402
100	22.335241971817386	30.7107461109427	21.848925287069594	25.10508663017032
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5960.0
1	5956.5
2	9397.5
3	13240.5
4	17499.5
5	30342.5
6	41232.0
7	47774.5
8	50841.0
9	46915.0
10	38343.5
11	29662.0
12	30600.0
13	42911.0
14	75521.0
15	123423.5
16	162184.0
17	182598.5
18	185970.0
19	173456.5
20	152241.5
21	132765.5
22	123917.0
23	133555.0
24	163687.5
25	212821.5
26	276878.5
27	347302.5
28	415982.5
29	489492.0
30	562524.0
31	633814.0
32	713525.0
33	788770.0
34	863399.0
35	931751.5
36	1004025.5
37	1074637.0
38	1121461.0
39	1157523.0
40	1194216.0
41	1229088.5
42	1252006.0
43	1273254.0
44	1299888.5
45	1326382.0
46	1332229.0
47	1323605.0
48	1313520.0
49	1287593.5
50	1257299.5
51	1219490.0
52	1161690.5
53	1097636.0
54	1032923.5
55	959319.5
56	881822.5
57	799794.5
58	714137.0
59	628230.5
60	529170.5
61	430430.5
62	341801.5
63	263035.5
64	197574.0
65	145546.5
66	107796.0
67	82112.0
68	62390.0
69	45452.0
70	32776.0
71	23806.0
72	16963.5
73	11853.0
74	8304.5
75	5746.5
76	3907.0
77	2582.0
78	1775.0
79	1225.5
80	816.5
81	569.0
82	423.0
83	320.5
84	258.5
85	197.5
86	144.5
87	102.5
88	62.5
89	36.0
90	28.5
91	23.5
92	20.0
93	14.0
94	7.5
95	5.0
96	5.5
97	6.5
98	8.5
99	10.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.06588288387395272
6	0.0
7	0.0
8	1.9272946155548948E-4
9	8.718713737034049E-4
10-11	4.588796703702131E-6
12-13	4.1911009893812797E-4
14-15	0.00614286918735592
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.013984039133892165
24-25	0.0
26-27	1.2243775092564853E-5
28-29	0.0027775859957237733
30-31	0.014041471034840936
32-33	0.0
34-35	2.770201234650988E-5
36-37	7.101275316490333E-5
38-39	6.357687105064547E-5
40-41	0.0
42-43	0.00480474648736808
44-45	0.02164304070727094
46-47	1.5931660314634043E-6
48-49	0.0027720051315179117
50-51	2.9443241091271994E-4
52-53	0.0
54-55	1.1466206044053425E-5
56-57	0.015017290163955239
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	1.53886919411797E-5
68-69	0.015760955044577835
70-71	0.023547351259448568
72-73	0.0
74-75	0.013215203498487784
76-77	0.0
78-79	0.01740705751365875
80-81	7.984854328310062E-5
82-83	0.0143390421629769
84-85	0.0
86-87	0.015221286022536348
88-89	0.030643736983758142
90-91	1.5226991715736124E-5
92-93	1.674372307652632E-4
94-95	0.01401963165067836
96-97	5.93481202472047E-6
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	682.0
20-21	2728.0
22-23	5240.0
24-25	7361.0
26-27	12924.0
28-29	25229.0
30-31	55175.0
32-33	69745.0
34-35	90735.0
36-37	133409.0
38-39	169803.0
40-41	180350.0
42-43	213991.0
44-45	269340.0
46-47	259858.0
48-49	231974.0
50-51	203055.0
52-53	184671.0
54-55	195190.0
56-57	209827.0
58-59	217004.0
60-61	221533.0
62-63	211368.0
64-65	217351.0
66-67	234057.0
68-69	245824.0
70-71	252013.0
72-73	230284.0
74-75	226506.0
76-77	237413.0
78-79	255220.0
80-81	265261.0
82-83	252028.0
84-85	241723.0
86-87	250271.0
88-89	268241.0
90-91	288543.0
92-93	292160.0
94-95	339414.0
96-97	1013065.0
98-99	2018833.0
100-101	2.2388909E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.9897809525967
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.79324026660335	66.90996015605896
2	9.117880276010736	14.222029709561584
3	2.510848004701803	5.874614576758744
4	1.0602205453125277	3.3074547236146667
5	0.5334186123907325	2.0800600368195656
6	0.3108445067318169	1.4545616994199422
7	0.1980644681275751	1.081290313863351
8	0.11844207563031274	0.7389817227183187
9	0.08236514750623747	0.5781275830925799
>10	0.2684544196768533	3.188499685030831
>50	0.004612517340422193	0.23969838947438044
>100	0.0015014328048373242	0.20096920739817717
>500	5.4226705986443316E-5	0.028961668271934922
>1k	5.3500456735388794E-5	0.09479052791696818
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.8355186814808526E-5	0.0	3.0591978024680875E-6	0.0
3	0.0	1.8355186814808526E-5	0.0	3.0591978024680875E-6	0.0
4	0.0	9.177593407404262E-5	0.0	3.0591978024680875E-6	0.0
5	0.0	9.177593407404262E-5	0.0	3.0591978024680875E-6	0.0
6	3.0591978024680875E-6	9.177593407404262E-5	0.0	3.0591978024680875E-6	9.177593407404263E-6
7	3.0591978024680875E-6	9.483513187651071E-5	0.0	1.5295989012340437E-5	9.177593407404263E-6
8	6.118395604936175E-6	1.0095352748144689E-4	0.0	1.5295989012340437E-5	9.177593407404263E-6
9	6.118395604936175E-6	2.0802545056782993E-4	0.0	2.7532780222212785E-5	1.5295989012340437E-5
10-11	1.223679120987235E-5	2.0802545056782993E-4	0.0	3.671037362961705E-5	1.5295989012340437E-5
12-13	1.223679120987235E-5	2.1261424727153205E-4	0.0	3.671037362961705E-5	1.8355186814808526E-5
14-15	1.3766390111106393E-5	3.365117582714896E-4	0.0	4.588796703702131E-5	2.7532780222212785E-5
16-17	1.5295989012340437E-5	3.6251493959246837E-4	0.0	5.9654357148127704E-5	3.365117582714896E-5
18-19	1.8355186814808526E-5	4.068733077282556E-4	0.0	6.424315385182984E-5	3.365117582714896E-5
20-21	1.8355186814808526E-5	4.588796703702131E-4	0.0	6.424315385182984E-5	3.365117582714896E-5
22-23	1.988478571604257E-5	5.292412198269791E-4	0.0	6.730235165429793E-5	4.282876923455322E-5
24-25	2.141438461727661E-5	5.93484373678809E-4	0.0	7.495034616046813E-5	5.506556044442557E-5
26-27	2.141438461727661E-5	6.271355495059579E-4	0.0	1.1013112088885114E-4	5.812475824689366E-5
28-29	2.2943983518510656E-5	6.28665148407192E-4	0.0	3.0286058244434065E-4	5.812475824689366E-5
30-31	2.7532780222212785E-5	6.913787033577878E-4	0.0	0.0010569528407527243	6.118395604936175E-5
32-33	2.7532780222212785E-5	7.097338901725963E-4	0.0	0.002805284384863236	8.565753846910644E-5
34-35	2.7532780222212785E-5	8.259834066663836E-4	0.0	0.005064501961985919	9.78943296789788E-5
36-37	2.7532780222212785E-5	9.46821719863873E-4	0.0	0.008678944165601964	9.78943296789788E-5
38-39	2.7532780222212785E-5	9.712953022836177E-4	0.0	0.018873720842326865	1.0401272528391496E-4
40-41	3.0591978024680874E-5	0.0010294200605305115	0.0	0.030616451607100616	1.0707192308638305E-4
42-43	3.671037362961705E-5	0.0010676600330613625	0.0	0.03784992481103641	1.1013112088885114E-4
44-45	3.671037362961705E-5	0.0011716727583452775	0.0	0.046256600372218716	1.1319031869131923E-4
46-47	3.671037362961705E-5	0.0012405047089008095	0.0	0.05506097164772187	1.2083831319748945E-4
48-49	3.671037362961705E-5	0.0012466231045057457	0.0	0.06367261346166954	1.3766390111106394E-4
50-51	3.671037362961705E-5	0.0013949941979254478	0.0	0.07261005984158006	1.422526978147661E-4
52-53	3.8239972530851096E-5	0.0014026421924316181	0.0	0.09327647059615322	1.575486868271065E-4
54-55	3.9769571432085134E-5	0.0014255861759501286	0.0	0.11575698564758996	1.7284467583944692E-4
56-57	3.9769571432085134E-5	0.0014424117638637032	0.0	0.13779850581437253	1.7590387364191504E-4
58-59	3.9769571432085134E-5	0.0014470005605674054	0.0	0.1512681537386395	1.789630714443831E-4
60-61	3.9769571432085134E-5	0.0015112437144192351	0.0	0.1621038323549815	1.8202226924685121E-4
62-63	4.282876923455322E-5	0.0015525428847525543	0.0	0.17103974913599077	1.927294615554895E-4
64-65	4.282876923455322E-5	0.001748331544110512	0.0	0.17572338097156942	1.927294615554895E-4
66-67	4.282876923455322E-5	0.0017758643243327246	0.0	0.18036877283461722	1.9731825825919163E-4
68-69	4.282876923455322E-5	0.0018141042968635758	0.0	0.18512582541745506	2.0190705496289378E-4
70-71	4.588796703702131E-5	0.0018431666759870227	0.0	0.189517303862898	2.0955504946906399E-4
72-73	4.89471648394894E-5	0.0018538738682956609	0.0	0.1929084246269339	2.2943983518510655E-4
74-75	4.89471648394894E-5	0.0019181170221474909	0.0	0.19563111067113048	2.2943983518510655E-4
76-77	4.89471648394894E-5	0.00202365934633264	0.0	0.19821919201201849	2.2943983518510655E-4
78-79	4.89471648394894E-5	0.002090961697986938	0.0	0.20002411871547465	2.2943983518510655E-4
80-81	5.200636264195748E-5	0.00209555049469064	0.0	0.20058089271552387	2.3555823079004273E-4
82-83	5.200636264195748E-5	0.002101668890295576	0.0	0.2009250524683015	2.493246209011491E-4
84-85	5.200636264195748E-5	0.0021139056815054486	0.0	0.20104894997930148	2.5085421980238317E-4
86-87	5.200636264195748E-5	0.0021521456540362994	0.0	0.20108413075402984	2.523838187036172E-4
88	5.200636264195748E-5	0.002337227121085619	0.0	0.2010871899518323	2.5697261540731935E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	42025	0.0	51.528908	1
GTATCAA	77330	0.0	38.599567	1
TCAACGC	94900	0.0	30.7985	4
ATCAACG	94940	0.0	30.767334	3
CAACGCA	96715	0.0	30.216055	5
AACGCAG	99860	0.0	29.332218	6
TATCAAC	100980	0.0	29.230215	2
ACGCAGA	114175	0.0	25.617058	7
CGCAGAG	115665	0.0	25.287174	8
GTACATG	98465	0.0	21.712002	1
GCAGAGT	136850	0.0	21.346483	9
TACATGG	97575	0.0	21.34374	2
ACATGGG	98220	0.0	20.592865	3
AGAGTAC	121940	0.0	19.805521	10-11
GAGTACT	80520	0.0	19.667063	12-13
CAGAGTA	132510	0.0	18.927422	10-11
CATGGGG	69745	0.0	18.477293	4
AGTACTT	86810	0.0	17.744986	12-13
GTACTTT	90900	0.0	17.345673	14-15
GTGGTAT	18770	0.0	15.617324	1
>>END_MODULE