FastQCFastQC Report
Fri 27 May 2016
SRR2139918_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139918_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32666323
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA466250.054.277641
GTATCAA873550.038.8022351
ATCAACG1089850.030.4988143
TCAACGC1094850.030.3791354
CAACGCA1110750.029.936545
TATCAAC1133700.029.5802632
AACGCAG1149500.029.027166
ACGCAGA1316100.025.2812187
CGCAGAG1330300.024.9985798
GTACATG1182500.022.1634851
TACATGG1180400.021.7338392
GCAGAGT1557400.021.344859
ACATGGG1187450.020.9795633
GAGTACT880650.019.8942712-13
AGAGTAC1416450.019.53306810-11
CAGAGTA1530000.018.85586510-11
CATGGGG793750.018.6127914
AGTACTT951200.017.87978212-13
GTACTTT1000900.017.47450814-15
GTGGTAT188950.016.8554121
Produced by FastQC (version 0.11.5)