##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139918_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32666323
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74348533197324	34.0	31.0	34.0	31.0	34.0
2	32.79719443783128	34.0	31.0	34.0	31.0	34.0
3	32.8839700752362	34.0	31.0	34.0	31.0	34.0
4	36.303155546462946	37.0	37.0	37.0	35.0	37.0
5	36.24806667098712	37.0	37.0	37.0	35.0	37.0
6	36.31682623110045	37.0	37.0	37.0	35.0	37.0
7	36.290384840681334	37.0	37.0	37.0	35.0	37.0
8	36.297686488926225	37.0	37.0	37.0	35.0	37.0
9	38.08664611563413	39.0	39.0	39.0	35.0	39.0
10-11	38.064188981416734	39.0	39.0	39.0	35.0	39.0
12-13	38.052366637653094	39.0	39.0	39.0	35.0	39.0
14-15	39.5488084012394	41.0	40.0	41.0	37.0	41.0
16-17	39.56585312647523	41.0	40.0	41.0	37.0	41.0
18-19	39.61593372477215	41.0	40.0	41.0	37.0	41.0
20-21	39.63239596562211	41.0	40.0	41.0	37.0	41.0
22-23	39.58953342563004	41.0	40.0	41.0	37.0	41.0
24-25	39.56386943213616	41.0	40.0	41.0	36.0	41.0
26-27	39.489997426577304	41.0	39.5	41.0	36.0	41.0
28-29	39.43788317238834	41.0	39.0	41.0	36.0	41.0
30-31	39.35541040440137	41.0	39.0	41.0	36.0	41.0
32-33	39.27452260196699	41.0	39.0	41.0	35.0	41.0
34-35	39.22381803677	41.0	39.0	41.0	35.0	41.0
36-37	39.17899124604806	41.0	39.0	41.0	35.0	41.0
38-39	39.135171056420496	41.0	39.0	41.0	35.0	41.0
40-41	39.10196103848791	41.0	39.0	41.0	35.0	41.0
42-43	39.030983307109196	41.0	39.0	41.0	35.0	41.0
44-45	38.65438747600737	40.5	38.5	41.0	34.5	41.0
46-47	38.7151520327512	40.0	38.0	41.0	34.5	41.0
48-49	38.8007934156055	40.0	38.0	41.0	35.0	41.0
50-51	38.767815619160544	40.0	38.0	41.0	35.0	41.0
52-53	38.67893066280084	40.0	38.0	41.0	34.5	41.0
54-55	38.56287285269835	40.0	38.0	41.0	34.0	41.0
56-57	38.40909946019985	40.0	37.5	41.0	34.0	41.0
58-59	38.25947581951611	40.0	37.0	41.0	34.0	41.0
60-61	38.08075981477603	40.0	37.0	41.0	34.0	41.0
62-63	37.8589988760351	39.5	36.0	41.0	33.5	41.0
64-65	37.61368600797479	39.0	36.0	41.0	33.0	41.0
66-67	37.354688374476254	39.0	35.0	41.0	33.0	41.0
68-69	36.99530038021521	38.0	35.0	40.5	32.5	41.0
70-71	36.68695980380099	37.0	35.0	40.0	32.0	41.0
72-73	36.406212612080196	37.0	35.0	39.5	32.0	41.0
74-75	36.07100832921508	36.0	35.0	39.0	32.0	41.0
76-77	35.734552157185725	36.0	35.0	39.0	32.0	40.5
78-79	35.39977596753821	35.5	35.0	37.5	31.5	39.5
80-81	35.08763085333066	35.0	34.0	37.0	31.0	39.0
82-83	34.79671096133954	35.0	34.0	37.0	31.0	39.0
84-85	34.54003820740883	35.0	34.0	36.0	31.0	37.5
86-87	34.317887765899215	35.0	34.0	36.0	31.0	37.0
88-89	34.26278992439558	35.0	34.0	36.0	31.0	37.0
90-91	34.22715413981945	35.0	34.0	35.0	31.0	36.5
92-93	34.14693420207911	35.0	34.0	35.0	31.0	36.0
94-95	34.18592080128546	35.0	34.0	35.0	32.0	36.0
96-97	34.230880798780525	35.0	34.0	35.0	32.0	36.0
98-99	34.33036115767513	35.0	35.0	35.0	32.5	36.0
100	34.11772474398493	35.0	34.0	35.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	24.0
20	103.0
21	510.0
22	2391.0
23	8353.0
24	23045.0
25	49734.0
26	92443.0
27	149544.0
28	219402.0
29	296735.0
30	386249.0
31	491516.0
32	622541.0
33	803860.0
34	1110715.0
35	1631852.0
36	2679898.0
37	5971374.0
38	1.0299199E7
39	7279128.0
40	547702.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.62091950171435	20.502084057639422	13.927398562733858	23.949597877912367
2	14.212557685173199	21.88437002842346	41.57154755372988	22.331524732673465
3	17.198651344995273	27.84082861116631	31.098495536213242	23.862024507625176
4	11.671074825287192	18.989933455320333	40.244737676781064	29.094254042611407
5	12.235535751089127	38.87228986406526	36.127241450379	12.764932934466607
6	27.919414131795612	39.712014725379404	19.632650421046776	12.735920721778204
7	24.969085133946663	33.53655383864294	24.454521557262506	17.039839470147893
8	23.97777878867453	36.84943972476071	21.837172941651794	17.33560854491297
9	25.733369831974816	16.2450128659548	21.415168699888348	36.606448602182034
10-11	23.501026531239116	27.005042073607182	29.82886013307528	19.665071262078417
12-13	24.80263887463486	24.849277299569376	29.641368573737424	20.706715252058338
14-15	21.90913940514613	25.82699780264861	27.429806065179672	24.834056727025587
16-17	21.147577583188657	28.972530823257948	27.9310668054069	21.948824788146496
18-19	21.21910843776326	28.073960145437855	29.81614888213773	20.890782534661156
20-21	22.298445380544397	27.19226715810511	29.540834080088878	20.96845338126161
22-23	21.968559239697786	27.167199785075614	29.42438660251373	21.439854372712873
24-25	21.574941071003412	27.350448197220555	29.528329172513633	21.546281559262397
26-27	21.56522361607469	27.46178974369083	29.43994864268813	21.53303799754636
28-29	21.516686652267268	27.462467829716093	29.477306910220424	21.54353860779621
30-31	21.85093763461423	27.341134573447917	29.31096631835764	21.496961473580214
32-33	21.653787938525802	27.52877058832662	29.25773119495875	21.559710278188827
34-35	21.784072717524797	27.496743225309206	29.27933753093008	21.439846526235925
36-37	21.757799907074478	27.458636932560964	29.208705930921237	21.57485722944332
38-39	21.668286111212883	27.5305370872378	29.232659847628963	21.568516953920362
40-41	21.858238989611543	27.390738173538054	29.140016797398633	21.611006039451773
42-43	21.781876186671738	27.50408756688456	28.909798743116244	21.804237503327457
44-45	22.020039488650507	27.636211397953765	28.619480413144387	21.72426870025134
46-47	22.014044321430287	27.686169225588465	28.29928639407123	22.00050005891002
48-49	21.97660911848761	27.815970777167603	28.1766133462943	22.030806758050485
50-51	21.954932685352123	27.965474425512436	27.996410124185545	22.083182764949893
52-53	22.081969218707197	27.83933666683574	27.964200016372242	22.114494098084815
54-55	22.051401496595304	27.754572989657415	28.05704359010551	22.13698192364177
56-57	22.044256192816416	27.843068776449424	28.000745054422225	22.111929976311934
58-59	22.014926777828418	27.90357173493655	28.003978943716028	22.077522543519006
60-61	22.10508513748152	27.88978292881733	27.980956967513226	22.024174966187925
62-63	22.037325658259466	27.957390544009513	27.94050312871027	22.06478066902075
64-65	22.009893460560235	27.919592738634492	27.911753780464743	22.15876002034053
66-67	22.120580689252346	27.952857508868668	27.790046873421737	22.136514928457252
68-69	22.11662662507538	27.92718731568215	27.816693701837885	22.13949235740458
70-71	22.09901187804693	27.682874278358266	27.937766835069876	22.280347008524924
72-73	22.107587116632015	27.72219696229039	27.860671910561123	22.309544010516472
74-75	22.027413956582418	27.887052916318154	27.822780998349256	22.262752128750172
76-77	22.143625226630707	27.855948210465815	27.75784956455683	22.24257699834665
78-79	22.11948730301498	27.844271589418323	27.720347681617685	22.315893425949014
80-81	22.115480743513537	27.878298269569395	27.70749086781361	22.298730119103453
82-83	22.15085500725282	27.829700199941236	27.817142472138954	22.202302320666984
84-85	22.21630484871905	27.85763805402766	27.736280136597536	22.189776960655756
86-87	22.058510089659578	27.937763478507726	27.805574507345458	22.198151924487235
88-89	22.13974079458939	27.90696482917988	27.731293490561644	22.222000885669082
90-91	22.214091716318688	27.841379471713033	27.804707180351567	22.13982163161671
92-93	22.2129881903063	27.8471624651778	27.75879398517293	22.181055359342967
94-95	22.04210882963875	27.91140789074892	27.819919346672194	22.226563932940138
96-97	22.125095963777156	28.07483104372614	27.868628949828043	21.93144404266866
98-99	23.185109158003804	29.986753462415667	28.05405885478604	18.774078524794486
100	22.12274698180292	30.917463918778893	21.9774602976187	24.982328801799486
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7202.0
1	7236.5
2	10984.0
3	15094.0
4	19760.0
5	34134.0
6	46297.0
7	54098.5
8	58101.0
9	53602.5
10	43611.5
11	33201.5
12	34020.5
13	48116.5
14	85442.5
15	140179.0
16	184451.0
17	207435.0
18	209833.0
19	194484.5
20	169723.0
21	146607.5
22	135746.0
23	145158.0
24	175805.0
25	226298.0
26	292947.5
27	365259.5
28	438322.0
29	519011.5
30	597895.0
31	673127.5
32	756479.0
33	835187.0
34	907422.0
35	972475.5
36	1035577.0
37	1095813.0
38	1134838.0
39	1160007.0
40	1190047.5
41	1212425.5
42	1225208.0
43	1248766.0
44	1279371.0
45	1307504.5
46	1313373.5
47	1310338.5
48	1305235.0
49	1280160.0
50	1251021.0
51	1215157.5
52	1161700.5
53	1098380.5
54	1028800.0
55	951796.0
56	873399.0
57	789037.5
58	700751.0
59	611959.5
60	515306.5
61	419041.0
62	328296.0
63	250961.0
64	188750.0
65	138287.5
66	100523.5
67	74631.0
68	55371.5
69	39404.0
70	28249.5
71	20671.0
72	15068.5
73	10900.0
74	7809.5
75	5435.5
76	3746.5
77	2497.0
78	1639.5
79	1084.5
80	746.0
81	548.5
82	415.5
83	294.0
84	218.0
85	159.5
86	119.5
87	96.0
88	62.0
89	36.0
90	22.0
91	15.0
92	13.5
93	11.5
94	8.5
95	7.0
96	7.0
97	8.0
98	8.5
99	9.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.06684253994549677
6	0.0
7	0.0
8	1.7449163164155328E-4
9	8.173555376893813E-4
10-11	3.0612566954658473E-6
12-13	3.8418771528096377E-4
14-15	0.0061163908775407625
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.014100625344593503
24-25	0.0
26-27	1.2252434769377398E-5
28-29	0.002831764459716427
30-31	0.01398610946305198
32-33	0.0
34-35	3.698339855304302E-5
36-37	7.576284799905448E-5
38-39	5.9041404991534394E-5
40-41	0.0
42-43	0.0050398907985908315
44-45	0.021818489699440436
46-47	0.0
48-49	0.0028163052771569786
50-51	3.3857424562077E-4
52-53	0.0
54-55	1.484396641008321E-5
56-57	0.015177498931853828
58-59	1.6731467080955639E-6
60-61	0.0
62-63	0.0
64-65	0.0
66-67	3.2800698330320156E-5
68-69	0.0162379717333219
70-71	0.023778738338910935
72-73	0.0
74-75	0.013588013505033268
76-77	0.0
78-79	0.017616831636764557
80-81	9.006591354407532E-5
82-83	0.014758073160863795
84-85	0.0
86-87	0.015682998165384404
88-89	0.03131589721153971
90-91	2.318584796123651E-5
92-93	1.6810840145779698E-4
94-95	0.014099503003894466
96-97	6.032769764047516E-6
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	650.0
20-21	2755.0
22-23	5497.0
24-25	8078.0
26-27	13666.0
28-29	28194.0
30-31	61251.0
32-33	76604.0
34-35	99319.0
36-37	145410.0
38-39	186474.0
40-41	197863.0
42-43	232792.0
44-45	295515.0
46-47	284636.0
48-49	253291.0
50-51	217334.0
52-53	192098.0
54-55	203006.0
56-57	221852.0
58-59	230915.0
60-61	237697.0
62-63	222116.0
64-65	226768.0
66-67	245898.0
68-69	256118.0
70-71	265784.0
72-73	238232.0
74-75	234599.0
76-77	245863.0
78-79	264462.0
80-81	277573.0
82-83	259727.0
84-85	247999.0
86-87	256011.0
88-89	277626.0
90-91	298941.0
92-93	300267.0
94-95	342401.0
96-97	1003793.0
98-99	1975635.0
100-101	2.2031613E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.08172033022359
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.61848789805133	65.99620339254226
2	8.915436134929609	13.74434309549227
3	2.6536943292507815	6.136539723876272
4	1.1213457967853881	3.457410524051321
5	0.587416810194226	2.2639549140331683
6	0.3358367611901709	1.5532125181601306
7	0.21254050432053226	1.1468091399015965
8	0.13792052387838344	0.8504920999513181
9	0.1013788861945232	0.7033013057732223
>10	0.30907100678877697	3.585593371972409
>50	0.0052132048547875835	0.2733709617245289
>100	0.0015731836300856635	0.19062790481900832
>500	4.7470571556279205E-5	0.024958533068551197
>1k	3.7489359932240926E-5	0.07318251463390761
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0612566954658473E-6	0.0	0.0	3.0612566954658473E-6	0.0
2	3.0612566954658473E-6	3.061256695465847E-5	0.0	9.183770086397542E-6	0.0
3	3.0612566954658473E-6	3.367382365012432E-5	0.0	1.8367540172795083E-5	0.0
4	3.0612566954658473E-6	1.0102147095037296E-4	0.0	3.979633704105601E-5	3.0612566954658473E-6
5	6.122513390931695E-6	1.040827276458388E-4	0.0	3.979633704105601E-5	3.0612566954658473E-6
6	6.122513390931695E-6	1.040827276458388E-4	0.0	3.979633704105601E-5	1.5306283477329235E-5
7	9.183770086397542E-6	1.040827276458388E-4	0.0	5.510262051838525E-5	1.5306283477329235E-5
8	9.183770086397542E-6	1.2245026781863388E-4	0.0	7.040890399571449E-5	1.8367540172795083E-5
9	9.183770086397542E-6	1.7755288833701914E-4	0.0	1.0714398434130465E-4	2.7551310259192626E-5
10-11	2.142879686826093E-5	1.7755288833701914E-4	0.0	1.2551152451409974E-4	2.7551310259192626E-5
12-13	3.6735080345590166E-5	1.80614145032485E-4	0.0	1.3775655129596315E-4	2.7551310259192626E-5
14-15	4.4388222084254784E-5	2.20410482073541E-4	0.0	1.5765471981649113E-4	3.826570869332309E-5
16-17	5.3571992170652324E-5	2.2959425215993856E-4	0.0	1.683691182506216E-4	4.898010712745356E-5
18-19	5.510262051838525E-5	2.525536773759324E-4	0.0	1.821447733802179E-4	5.20413638229194E-5
20-21	5.969450556158402E-5	2.770437309396592E-4	0.0	2.0510419859621175E-4	5.20413638229194E-5
22-23	7.19395323434474E-5	3.1837069632844814E-4	0.0	2.4490053563726777E-4	5.81638772138511E-5
24-25	9.03070725162425E-5	3.4898326328310655E-4	0.0	3.076562978943176E-4	7.653141738664618E-5
26-27	1.040827276458388E-4	3.6888143180363456E-4	0.0	3.826570869332309E-4	7.959267408211202E-5
28-29	1.0714398434130465E-4	3.7041206015136753E-4	0.0	7.255178368254058E-4	7.959267408211202E-5
30-31	1.1938901112316804E-4	4.132696538878894E-4	0.0	0.0019102241779706886	8.41845591253108E-5
32-33	1.362259229482302E-4	4.163309105833552E-4	0.0	0.004379127702863894	8.877644416850957E-5
34-35	1.5765471981649113E-4	5.00515469708666E-4	0.0	0.007310280988772443	1.0102147095037296E-4
36-37	1.7755288833701914E-4	5.908225422249085E-4	0.0	0.012209822329865533	1.0102147095037296E-4
38-39	1.8673665842341665E-4	6.306188792659645E-4	0.0	0.027381410512594268	1.0561335599357173E-4
40-41	1.9285917181434837E-4	7.102115533480765E-4	0.0	0.04459179565450326	1.0714398434130465E-4
42-43	1.9898168520528006E-4	7.362322352595363E-4	0.0	0.05434189822956199	1.0867461268903758E-4
44-45	2.0510419859621175E-4	8.188861660371142E-4	0.0	0.06541752495375741	1.1326649773223635E-4
46-47	2.1122671198714346E-4	8.755194149032323E-4	0.0	0.07733193601251051	1.2245026781863388E-4
48-49	2.1122671198714346E-4	8.785806715986982E-4	0.0	0.08881164862050743	1.438790646868948E-4
50-51	2.142879686826093E-4	9.688877441149407E-4	0.0	0.10068779397056718	1.438790646868948E-4
52-53	2.20410482073541E-4	9.704183724626736E-4	0.0	0.13306517541016172	1.5765471981649113E-4
54-55	2.20410482073541E-4	9.857246559400027E-4	0.0	0.16916657561979043	1.7755288833701914E-4
56-57	2.20410482073541E-4	0.001004092196112798	0.0	0.20198936990857524	1.821447733802179E-4
58-59	2.20410482073541E-4	0.0010132759661991954	0.0	0.2175420845498895	1.8367540172795082E-4
60-61	2.2347173876900684E-4	0.001037766019762922	0.0	0.22863760944260547	1.8367540172795082E-4
62-63	2.2347173876900684E-4	0.001060725444978916	0.0	0.23736372165303088	1.9592042850981423E-4
64-65	2.2653299546447267E-4	0.0011755225710588853	0.0	0.24308061853181334	1.9898168520528006E-4
66-67	2.326555088554044E-4	0.0011923594828839473	0.0	0.2486214931506065	2.00512313553013E-4
68-69	2.4183927894180193E-4	0.0012275639348818049	0.0	0.2539128753487192	2.0204294190074592E-4
70-71	2.4490053563726777E-4	0.0012505233600977987	0.0	0.2589532344978037	2.0510419859621178E-4
72-73	2.464311639850007E-4	0.0012520539884455315	0.0	0.26269409017966305	2.1734922537807515E-4
74-75	2.479617923327336E-4	0.0012918503254865875	0.0	0.2655349363930553	2.1734922537807515E-4
76-77	2.510230490281995E-4	0.0013484835743527057	0.0	0.2682043522315015	2.2194111042127393E-4
78-79	2.6020681911459703E-4	0.0013729736279164324	0.0	0.26996151357469894	2.2347173876900684E-4
80-81	2.6020681911459703E-4	0.001380626769655097	0.0	0.27055233611692386	2.2653299546447267E-4
82-83	2.663293325055287E-4	0.0013928717964369606	0.0	0.2710436678165461	2.3877802224633608E-4
84-85	2.724518458964604E-4	0.0014112393366097556	0.0	0.2712319351033172	2.3877802224633608E-4
86-87	2.724518458964604E-4	0.0014326681334780164	0.0	0.27127785395374926	2.4030865059406902E-4
88	2.724518458964604E-4	0.001542873374514787	0.0	0.27128550709548793	2.479617923327336E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	46625	0.0	54.27764	1
GTATCAA	87355	0.0	38.802235	1
ATCAACG	108985	0.0	30.498814	3
TCAACGC	109485	0.0	30.379135	4
CAACGCA	111075	0.0	29.93654	5
TATCAAC	113370	0.0	29.580263	2
AACGCAG	114950	0.0	29.02716	6
ACGCAGA	131610	0.0	25.281218	7
CGCAGAG	133030	0.0	24.998579	8
GTACATG	118250	0.0	22.163485	1
TACATGG	118040	0.0	21.733839	2
GCAGAGT	155740	0.0	21.34485	9
ACATGGG	118745	0.0	20.979563	3
GAGTACT	88065	0.0	19.89427	12-13
AGAGTAC	141645	0.0	19.533068	10-11
CAGAGTA	153000	0.0	18.855865	10-11
CATGGGG	79375	0.0	18.612791	4
AGTACTT	95120	0.0	17.879782	12-13
GTACTTT	100090	0.0	17.474508	14-15
GTGGTAT	18895	0.0	16.855412	1
>>END_MODULE