FastQCFastQC Report
Fri 27 May 2016
SRR2139917_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139917_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34889224
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA450750.051.1162221
GTATCAA822550.038.288811
TCAACGC1013400.030.386954
ATCAACG1014250.030.3614853
CAACGCA1034550.029.7491115
TATCAAC1069400.029.096972
AACGCAG1064700.028.9691376
ACGCAGA1221700.025.2183847
CGCAGAG1235700.024.9223148
CGAGACC274600.022.99082486-87
GAGACCG273000.022.9155388-89
AGACCGA280400.022.74456488-89
GACCGAG283700.022.4451290-91
ACCGAGG290300.022.29282890-91
ACGAGAC288500.021.69373786-87
GTACATG1064400.021.6142961
ATCTCCG275550.021.45495874-75
CACGAGA294300.021.22777784-85
TACATGG1059150.021.2182962
GCAGAGT1463450.021.0550179
Produced by FastQC (version 0.11.5)