##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139917_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34889224
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.722624269315936	34.0	31.0	34.0	31.0	34.0
2	32.776552181269494	34.0	31.0	34.0	31.0	34.0
3	32.86536464668862	34.0	31.0	34.0	31.0	34.0
4	36.29101487037946	37.0	37.0	37.0	35.0	37.0
5	36.23294338676033	37.0	37.0	37.0	35.0	37.0
6	36.30117729187671	37.0	37.0	37.0	35.0	37.0
7	36.273788519916636	37.0	37.0	37.0	35.0	37.0
8	36.281225114092535	37.0	37.0	37.0	35.0	37.0
9	38.06814895051836	39.0	39.0	39.0	35.0	39.0
10-11	38.043684763524695	39.0	39.0	39.0	35.0	39.0
12-13	38.03277213904213	39.0	39.0	39.0	35.0	39.0
14-15	39.527517364673976	41.0	39.0	41.0	36.0	41.0
16-17	39.54252529376979	41.0	39.5	41.0	36.0	41.0
18-19	39.587921803591854	41.0	40.0	41.0	37.0	41.0
20-21	39.602033965159706	41.0	40.0	41.0	37.0	41.0
22-23	39.56160830660809	41.0	40.0	41.0	36.5	41.0
24-25	39.53601605390627	41.0	39.5	41.0	36.0	41.0
26-27	39.46094410598779	41.0	39.0	41.0	36.0	41.0
28-29	39.40763861351518	41.0	39.0	41.0	36.0	41.0
30-31	39.328775001544955	41.0	39.0	41.0	36.0	41.0
32-33	39.251779150789254	41.0	39.0	41.0	35.0	41.0
34-35	39.20072865969702	41.0	39.0	41.0	35.0	41.0
36-37	39.15455706085599	41.0	39.0	41.0	35.0	41.0
38-39	39.10955666116222	41.0	39.0	41.0	35.0	41.0
40-41	39.07520292657675	41.0	39.0	41.0	35.0	41.0
42-43	39.00262050696441	41.0	39.0	41.0	35.0	41.0
44-45	38.62643999235506	40.5	38.0	41.0	34.5	41.0
46-47	38.68563505486307	40.0	38.0	41.0	34.5	41.0
48-49	38.76831362410586	40.0	38.0	41.0	35.0	41.0
50-51	38.7326883420254	40.0	38.0	41.0	35.0	41.0
52-53	38.64053156053555	40.0	38.0	41.0	34.0	41.0
54-55	38.520348736002774	40.0	38.0	41.0	34.0	41.0
56-57	38.36733935952896	40.0	37.0	41.0	34.0	41.0
58-59	38.21764515665234	40.0	37.0	41.0	34.0	41.0
60-61	38.04033203299325	40.0	36.5	41.0	34.0	41.0
62-63	37.813019535590065	39.0	36.0	41.0	33.0	41.0
64-65	37.57042616152696	39.0	35.5	41.0	33.0	41.0
66-67	37.31291234764054	39.0	35.0	41.0	33.0	41.0
68-69	36.952002583977105	38.0	35.0	40.0	32.5	41.0
70-71	36.64420760563644	37.0	35.0	40.0	32.0	41.0
72-73	36.360288303796864	37.0	35.0	39.0	32.0	41.0
74-75	36.023716294298815	36.0	35.0	39.0	32.0	41.0
76-77	35.68501534331183	36.0	35.0	39.0	32.0	40.0
78-79	35.3533489515757	35.5	35.0	37.5	31.0	39.5
80-81	35.03673071628186	35.0	34.0	37.0	31.0	39.0
82-83	34.742277539500776	35.0	34.0	36.5	31.0	39.0
84-85	34.49094509054049	35.0	34.0	36.0	31.0	37.5
86-87	34.26696536974396	35.0	34.0	36.0	31.0	37.0
88-89	34.215611920188266	35.0	34.0	36.0	31.0	37.0
90-91	34.18196073062178	35.0	34.0	35.0	31.0	36.0
92-93	34.10854619854869	35.0	34.0	35.0	31.0	36.0
94-95	34.14644475037779	35.0	34.0	35.0	32.0	36.0
96-97	34.19566377533254	35.0	34.0	35.0	32.0	36.0
98-99	34.30417089024502	35.0	35.0	35.0	32.0	36.0
100	34.086712708434334	35.0	34.0	35.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	22.0
20	108.0
21	598.0
22	2662.0
23	9649.0
24	25567.0
25	55682.0
26	102384.0
27	166348.0
28	241646.0
29	328371.0
30	425267.0
31	536248.0
32	679086.0
33	876527.0
34	1210232.0
35	1768095.0
36	2921152.0
37	6410890.0
38	1.1006335E7
39	7601096.0
40	521257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.36806826084754	20.519332846153297	13.925950316349828	24.18664857664934
2	14.2111071315315	21.78808562781448	41.532480057452695	22.46832718320132
3	17.257784237333567	27.55308057295857	31.036124506523848	24.15301068318401
4	11.834238560307332	18.898760832284488	40.135790925014554	29.13120968239362
5	12.410291698272014	38.670022505177585	36.17773764212153	12.741948154428878
6	28.229404013113047	39.57948448495157	19.547333583572968	12.643777918362414
7	25.084220847101673	33.321985034691515	24.436192676569704	17.15760144163711
8	23.920781957199157	36.969182387900105	21.84905615274974	17.260979502150995
9	25.612835381026372	16.328259012305246	21.388728467135586	36.6701771395328
10-11	23.5487466587819	26.907844204723162	29.862301028094006	19.681108108400927
12-13	24.722728123019213	24.66204582341243	29.68252837459645	20.932697678971905
14-15	21.91076621008112	25.741884692396198	27.31508753971148	25.032261557811204
16-17	21.334667689943462	28.88090890184316	27.800773098306802	21.983650309906576
18-19	21.402774679081425	28.04991592819605	29.57429061764171	20.97301877508081
20-21	22.323139738235717	27.221740409934625	29.317721793537153	21.1373980582925
22-23	22.012919898863316	27.15652705182667	29.225454012711754	21.605099036598258
24-25	21.745232548683024	27.308909473792735	29.290978562121783	21.654879415402455
26-27	21.75409462811028	27.444333617918414	29.144966803234812	21.656604950736497
28-29	21.691274380512045	27.41900226483957	29.192734435745216	21.696988918903166
30-31	22.031627608838466	27.286037201688256	29.072999600468385	21.609335589004896
32-33	21.826178999270333	27.484841610124676	29.010461086024907	21.67851830458008
34-35	21.893878466768072	27.49247335443693	29.04611333792629	21.567534840868714
36-37	21.91555849507608	27.41387026104543	28.968528935713362	21.70204230816513
38-39	21.78024386143446	27.530742901115623	28.974771750273987	21.714241487175922
40-41	22.018040730281896	27.383544499970025	28.883209269114495	21.71520550063358
42-43	21.885908295161986	27.520982643689546	28.673884135885196	21.919224925263272
44-45	22.135865664245436	27.586798682474356	28.445988251928927	21.831347401351277
46-47	22.105568838435065	27.64288723932446	28.141230003579633	22.110313918660847
48-49	22.056356095013367	27.77453942071807	28.05373730141339	22.115367182855174
50-51	22.045037153370455	27.911259985761088	27.88480224333637	22.158900617532083
52-53	22.201170130308537	27.761206414783345	27.860566619028333	22.177056835879785
54-55	22.117712839221902	27.66768342199138	27.99283382160417	22.221769917182552
56-57	22.128578962870204	27.746632659544062	27.948872944743382	22.175915432842356
58-59	22.084583217554833	27.793348807561923	27.839627385627175	22.282440589256076
60-61	22.30610527759003	27.733263734090706	27.974012850051704	21.986618138267556
62-63	22.058215959106185	27.787037154934964	27.917752332580513	22.236994553378338
64-65	22.04004144229184	27.725735599002512	27.902748759405156	22.331474199300498
66-67	22.102262515544066	27.81901396050248	27.92158960815017	22.157133915803286
68-69	22.090885152129466	27.955512610340406	27.826713793030443	22.126888444499677
70-71	22.085879380340025	27.68172673116644	27.73086164046682	22.501532248026713
72-73	22.128078413579626	27.724889508220503	27.63954459001531	22.50748748818456
74-75	21.99617155648412	27.89056352452367	27.837301443973605	22.2759634750186
76-77	22.15408346294821	27.666193493922712	27.741128633491684	22.43859440963739
78-79	22.102718867243436	27.697275261591557	27.5385228987575	22.661482972407512
80-81	22.307160097585978	27.89840907233716	27.510457165105656	22.283973664971203
82-83	22.350854718068465	27.657797042240922	27.63934191621667	22.35200632347394
84-85	22.227876496160736	27.650973891925645	27.558891167378913	22.562258444534702
86-87	22.06954628494869	27.891298319717333	27.649383775192042	22.389771620141936
88-89	22.354539291270857	27.867317046534318	27.57891218342714	22.199231478767683
90-91	22.395144629940877	27.855930496924756	27.619969290990536	22.128955582143828
92-93	22.2448234902068	27.626103454863028	27.58068336355147	22.548389691378702
94-95	22.256071551681234	27.891977533185003	27.661191286827936	22.190759628305827
96-97	22.541252642174914	27.79944088580486	27.731179099926447	21.92812737209378
98-99	23.30198178852267	29.87514755853199	27.98903103927568	18.83383961366966
100	22.237541726582002	30.763809418871528	21.915992131082472	25.08265672346399
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8600.0
1	8392.0
2	11182.0
3	14496.0
4	19092.0
5	33137.5
6	44800.0
7	51654.5
8	54893.5
9	51177.5
10	42980.0
11	33726.0
12	33854.5
13	45870.5
14	80013.0
15	131442.0
16	173722.0
17	196133.5
18	199600.5
19	186811.5
20	165184.5
21	145273.0
22	136240.0
23	146530.5
24	179019.0
25	231344.5
26	300684.5
27	377281.0
28	452281.5
29	534432.5
30	614558.5
31	692325.5
32	781567.5
33	863832.5
34	940806.5
35	1014435.5
36	1089553.5
37	1164385.0
38	1216482.5
39	1252179.5
40	1289120.5
41	1317147.0
42	1336729.0
43	1365613.5
44	1389723.5
45	1411967.0
46	1427799.0
47	1427760.5
48	1415857.0
49	1381393.5
50	1340658.0
51	1296756.5
52	1236920.0
53	1166117.5
54	1093571.0
55	1015834.0
56	934398.0
57	849542.0
58	760394.5
59	669851.5
60	564478.5
61	457512.0
62	360936.5
63	278898.5
64	212434.0
65	156324.5
66	114061.0
67	85534.5
68	64504.0
69	47544.5
70	34959.0
71	25391.5
72	18521.0
73	13436.5
74	9454.0
75	6563.0
76	4584.5
77	3141.0
78	2112.5
79	1444.5
80	1026.5
81	755.0
82	566.0
83	427.5
84	337.5
85	242.5
86	179.5
87	126.0
88	71.0
89	46.0
90	35.0
91	28.5
92	23.5
93	16.0
94	7.5
95	4.0
96	3.0
97	2.0
98	4.5
99	7.5
100	14.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.06599458904560332
6	0.0
7	0.0
8	1.4617693990557086E-4
9	8.2260356378233E-4
10-11	5.732429015904739E-6
12-13	3.9410449484345077E-4
14-15	0.0060606105770652854
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.013755460421145844
24-25	0.0
26-27	2.0075533333788378E-5
28-29	0.0026914851824702134
30-31	0.013810906117521905
32-33	0.0
34-35	3.6065794800602104E-5
36-37	8.684807869686542E-5
38-39	7.12736696402284E-5
40-41	0.0
42-43	0.005040758352715513
44-45	0.021324110615914906
46-47	2.9956313659319636E-6
48-49	0.0027360187440398868
50-51	3.28912435500043E-4
52-53	0.0
54-55	1.2344942697476456E-5
56-57	0.015241887859080363
58-59	1.5629902172286005E-6
60-61	0.0
62-63	0.0
64-65	0.0
66-67	3.0601317499460046E-5
68-69	0.015876362369125603
70-71	0.022755338553897497
72-73	0.0
74-75	0.013311245562104957
76-77	0.0
78-79	0.01752553055089224
80-81	6.514001666590184E-5
82-83	0.014430217311384904
84-85	0.0
86-87	0.015287155948573761
88-89	0.03074842894662339
90-91	2.8812422879498415E-5
92-93	1.7303208920164834E-4
94-95	0.014011223945104312
96-97	3.746736007509958E-6
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	771.0
20-21	2828.0
22-23	5824.0
24-25	8543.0
26-27	14758.0
28-29	28549.0
30-31	64001.0
32-33	80988.0
34-35	105332.0
36-37	155514.0
38-39	200326.0
40-41	211425.0
42-43	244863.0
44-45	307271.0
46-47	294888.0
48-49	265972.0
50-51	235008.0
52-53	207689.0
54-55	217452.0
56-57	239555.0
58-59	145256.0
60-61	259668.0
62-63	239878.0
64-65	243813.0
66-67	262685.0
68-69	273700.0
70-71	280123.0
72-73	256366.0
74-75	250186.0
76-77	262116.0
78-79	281900.0
80-81	294286.0
82-83	276725.0
84-85	263888.0
86-87	271255.0
88-89	291529.0
90-91	313644.0
92-93	317894.0
94-95	366038.0
96-97	1186294.0
98-99	2118176.0
100-101	2.3542247E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.97714909652746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.62104524319997	65.90863965486328
2	9.06889513563419	13.961953859929713
3	2.5522213185320117	5.893881628919235
4	1.134008390835855	3.4917093191233914
5	0.5439490801599427	2.0935824722195435
6	0.3062489565995557	1.4144502955691978
7	0.19761601939461676	1.0648342452161172
8	0.13967727748544007	0.8601566891515006
9	0.09587601068966374	0.6642235772674655
>10	0.3323615136518903	3.9057483058793934
>50	0.005970636277000573	0.3058816054190148
>100	0.001956529394992265	0.2594320962641508
>500	1.1007389516191593E-4	0.06419900272417606
>1k	6.381424960459839E-5	0.1113072474537159
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1464858031809478E-5
2	0.0	3.439457409542843E-5	0.0	1.7197287047714216E-5	1.1464858031809478E-5
3	0.0	3.7260788603380804E-5	0.0	2.2929716063618956E-5	1.1464858031809478E-5
4	0.0	1.1751479482604714E-4	0.0	2.2929716063618956E-5	1.7197287047714216E-5
5	0.0	1.1751479482604714E-4	0.0	2.5795930571571324E-5	1.7197287047714216E-5
6	5.732429015904739E-6	1.1751479482604714E-4	0.0	2.8662145079523696E-5	2.2929716063618956E-5
7	5.732429015904739E-6	1.1751479482604714E-4	0.0	4.0127003111333176E-5	3.152835958747606E-5
8	5.732429015904739E-6	1.3471208187376137E-4	0.0	4.0127003111333176E-5	4.0127003111333176E-5
9	5.732429015904739E-6	2.8375523628728456E-4	0.0	5.732429015904739E-5	4.585943212723791E-5
10-11	1.7197287047714216E-5	2.8375523628728456E-4	0.0	7.882089896869016E-5	4.585943212723791E-5
12-13	2.2929716063618956E-5	2.8375523628728456E-4	0.0	1.031837222862853E-4	4.72925393812141E-5
14-15	3.2961466841452253E-5	3.869389585735699E-4	0.0	1.0891615130219004E-4	7.308846995278542E-5
16-17	5.875739741302357E-5	4.0127003111333176E-4	0.0	1.4331072539761847E-4	9.45850787624282E-5
18-19	6.735604093688067E-5	4.3566460520876016E-4	0.0	1.805715140009993E-4	1.1894790208002333E-4
20-21	7.165536269880924E-5	4.8725646635190277E-4	0.0	1.9203637203280878E-4	1.4044451088966612E-4
22-23	9.3151971508452E-5	5.632111508126407E-4	0.0	2.0923365908052298E-4	1.5620869068340413E-4
24-25	1.2324722384195189E-4	6.391658352733784E-4	0.0	2.3359648239811812E-4	1.7627219223907074E-4
26-27	1.3614518912773756E-4	6.807259456386878E-4	0.0	2.937869870651179E-4	1.777052994930469E-4
28-29	1.4044451088966612E-4	6.835921601466401E-4	0.0	5.861408668762595E-4	1.8487083576292784E-4
30-31	1.4474383265159467E-4	7.796103461630444E-4	0.0	0.0017125631685015407	1.9203637203280878E-4
32-33	1.6624044146123742E-4	7.91075204194854E-4	0.0	0.004009834096625365	1.977688010487135E-4
34-35	2.1783230260438008E-4	9.171886425447583E-4	0.0	0.006777164204053378	2.2643094612823718E-4
36-37	2.593924129696895E-4	0.0010447351881486388	0.0	0.01179877202198593	2.2643094612823718E-4
38-39	2.6082552022366564E-4	0.0010676649042122577	0.0	0.029014689464001837	2.3216337514414193E-4
40-41	2.679910564935466E-4	0.0011808803772763762	0.0	0.048874689789603805	2.3789580416004667E-4
42-43	2.7515659276342746E-4	0.0012167080586257808	0.0	0.05939226392653502	2.3789580416004667E-4
44-45	2.851883435412608E-4	0.0013643181057853278	0.0	0.07192048754079483	2.4219512592197522E-4
46-47	2.9522009431909405E-4	0.001481832900611375	0.0	0.08508501077582006	2.507937694458323E-4
48-49	2.9808630882704643E-4	0.0014947308658971605	0.0	0.09724492582580799	2.7515659276342746E-4
50-51	3.009525233349988E-4	0.0016423409130567079	0.0	0.10969146232659116	2.7802280727137985E-4
52-53	3.1814981038271297E-4	0.0016538057710885172	0.0	0.14789236928858035	3.2244913214464157E-4
54-55	3.267484539065701E-4	0.0016724361653902076	0.0	0.19127395897369343	3.697416715258557E-4
56-57	3.3248088292247484E-4	0.0017082638467396122	0.0	0.2303418958243382	3.869389585735699E-4
58-59	3.3391399017645106E-4	0.0017168624902634694	0.0	0.4012972601511573	3.984038166053793E-4
60-61	3.41079526446332E-4	0.001761288815136731	0.0	0.5645482398806003	4.127348891451412E-4
62-63	3.41079526446332E-4	0.001792817174724207	0.0	0.5719244429168158	4.3853081971671255E-4
64-65	3.41079526446332E-4	0.001977688010487135	0.0	0.5784565457804393	4.4426324873261726E-4
66-67	3.439457409542843E-4	0.0019934521902808732	0.0	0.5845587164678698	4.571612140184029E-4
68-69	3.6114302800199853E-4	0.0020593751239637776	0.0	0.590560569647522	4.7005917930418857E-4
70-71	3.640092425099509E-4	0.0020980690198211345	0.0	0.5952224675447066	4.872564663519028E-4
72-73	3.6544234976392713E-4	0.0021124000923608962	0.0	0.5987565100330119	5.546125072887836E-4
74-75	3.72607886033808E-4	0.002174023704281872	0.0	0.6015568016072814	5.617780435586645E-4
76-77	3.812065295576651E-4	0.0022929716063618956	0.0	0.604285437818852	5.675104725745691E-4
78-79	3.869389585735699E-4	0.002351729003774919	0.0	0.6060983184951318	5.718097943364978E-4
80-81	3.8980517308152227E-4	0.0023545952182828715	0.0	0.6067116883998337	5.933064031461404E-4
82-83	3.9983692385935554E-4	0.002367493183568657	0.0	0.6070484686045181	6.248347627336165E-4
84-85	4.141679963991174E-4	0.002381824256108419	0.0	0.6071917793299157	6.277009772415688E-4
86-87	4.156011036530936E-4	0.002430549902743609	0.0	0.607231906333027	6.405989425273546E-4
88	4.156011036530936E-4	0.0026283187037923227	0.0	0.607236205654789	6.534969078131402E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	45075	0.0	51.116222	1
GTATCAA	82255	0.0	38.28881	1
TCAACGC	101340	0.0	30.38695	4
ATCAACG	101425	0.0	30.361485	3
CAACGCA	103455	0.0	29.749111	5
TATCAAC	106940	0.0	29.09697	2
AACGCAG	106470	0.0	28.969137	6
ACGCAGA	122170	0.0	25.218384	7
CGCAGAG	123570	0.0	24.922314	8
CGAGACC	27460	0.0	22.990824	86-87
GAGACCG	27300	0.0	22.91553	88-89
AGACCGA	28040	0.0	22.744564	88-89
GACCGAG	28370	0.0	22.44512	90-91
ACCGAGG	29030	0.0	22.292828	90-91
ACGAGAC	28850	0.0	21.693737	86-87
GTACATG	106440	0.0	21.614296	1
ATCTCCG	27555	0.0	21.454958	74-75
CACGAGA	29430	0.0	21.227777	84-85
TACATGG	105915	0.0	21.218296	2
GCAGAGT	146345	0.0	21.055017	9
>>END_MODULE