FastQCFastQC Report
Fri 27 May 2016
SRR2139916_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139916_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32110960
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA386650.051.0180131
GTATCAA701400.039.080071
ATCTCGT260800.037.65574694
TCAACGC856050.031.3043374
ATCAACG860400.031.1511573
CAACGCA872600.030.69565
AACGCAG902750.029.7729056
TATCAAC913350.029.637342
TATCTCG312250.029.37831594
ACGCAGA1040000.025.8060727
CGCAGAG1054950.025.4238138
GTACATG1022950.023.3885361
TACATGG1018250.022.9977022
TATGCCG148100.022.77283186-87
TCGTATG157950.022.15750184-85
ACATGGG1030050.022.101093
CGCTATC455800.021.81897294
GCCGTCT165450.021.6926190-91
TCTCGTA160300.021.38671982-83
CTCGTAT155100.021.26201882-83
Produced by FastQC (version 0.11.5)