##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139916_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32110960
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.791700528417714	34.0	31.0	34.0	31.0	34.0
2	32.8480706275988	34.0	31.0	34.0	31.0	34.0
3	32.93661042833973	34.0	31.0	34.0	31.0	34.0
4	36.33830981695969	37.0	37.0	37.0	35.0	37.0
5	36.2820006938441	37.0	37.0	37.0	35.0	37.0
6	36.34668353110589	37.0	37.0	37.0	35.0	37.0
7	36.32226034350888	37.0	37.0	37.0	35.0	37.0
8	36.32831696716635	37.0	37.0	37.0	35.0	37.0
9	38.13095351244559	39.0	39.0	39.0	37.0	39.0
10-11	38.10727377817418	39.0	39.0	39.0	36.0	39.0
12-13	38.096786268613585	39.0	39.0	39.0	35.0	39.0
14-15	39.616121255795534	41.0	40.0	41.0	37.0	41.0
16-17	39.630707724091714	41.0	40.0	41.0	37.0	41.0
18-19	39.671640041281854	41.0	40.0	41.0	37.0	41.0
20-21	39.68485390456362	41.0	40.0	41.0	37.0	41.0
22-23	39.64444657707003	41.0	40.0	41.0	37.0	41.0
24-25	39.621380171333826	41.0	40.0	41.0	37.0	41.0
26-27	39.55216779238831	41.0	40.0	41.0	36.5	41.0
28-29	39.50324063661215	41.0	40.0	41.0	36.0	41.0
30-31	39.43049989119843	41.0	39.0	41.0	36.0	41.0
32-33	39.36073795714201	41.0	39.0	41.0	36.0	41.0
34-35	39.31136328998885	41.0	39.0	41.0	35.0	41.0
36-37	39.26682807615486	41.0	39.0	41.0	35.0	41.0
38-39	39.22249830519554	41.0	39.0	41.0	35.0	41.0
40-41	39.186991279962115	41.0	39.0	41.0	35.0	41.0
42-43	39.1125439802034	41.0	39.0	41.0	35.0	41.0
44-45	38.738135974634716	41.0	38.5	41.0	34.5	41.0
46-47	38.796486506956214	41.0	38.0	41.0	34.5	41.0
48-49	38.876729004953525	40.0	38.0	41.0	35.0	41.0
50-51	38.83657138702047	40.0	38.0	41.0	35.0	41.0
52-53	38.74211264472622	40.0	38.0	41.0	35.0	41.0
54-55	38.621519455657435	40.0	38.0	41.0	34.5	41.0
56-57	38.46280410030522	40.0	37.5	41.0	34.0	41.0
58-59	38.31153990206663	40.0	37.0	41.0	34.0	41.0
60-61	38.128806849815774	40.0	37.0	41.0	34.0	41.0
62-63	37.90440077439237	39.5	36.0	41.0	34.0	41.0
64-65	37.662443003795474	39.0	36.0	41.0	33.0	41.0
66-67	37.39947831629428	39.0	35.0	41.0	33.0	41.0
68-69	37.033089692978436	38.0	35.0	40.5	33.0	41.0
70-71	36.716730979728254	37.0	35.0	40.0	32.0	41.0
72-73	36.43305366647107	37.0	35.0	39.5	32.0	41.0
74-75	36.085992425749275	36.0	35.0	39.0	32.0	41.0
76-77	35.73984072072041	36.0	35.0	39.0	32.0	40.5
78-79	35.395165992668325	35.5	35.0	37.5	32.0	39.5
80-81	35.06910056158807	35.0	34.5	37.0	31.0	39.0
82-83	34.76872548832434	35.0	34.0	36.5	31.0	39.0
84-85	34.517288925370835	35.0	34.0	36.0	31.0	37.0
86-87	34.29145683318727	35.0	34.0	36.0	31.0	37.0
88-89	34.24045850485078	35.0	34.0	35.5	31.0	37.0
90-91	34.21516398135232	35.0	34.0	35.0	31.0	36.0
92-93	34.13805911888282	35.0	34.0	35.0	31.0	36.0
94-95	34.17039165550044	35.0	34.0	35.0	32.0	36.0
96-97	34.216044864608804	35.0	34.0	35.0	32.0	36.0
98-99	34.323487454227006	35.0	35.0	35.0	32.5	36.0
100	34.0991200380988	35.0	34.0	35.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	23.0
20	96.0
21	485.0
22	2214.0
23	7993.0
24	21398.0
25	47048.0
26	87717.0
27	142964.0
28	210427.0
29	285035.0
30	369130.0
31	466917.0
32	591347.0
33	765416.0
34	1066946.0
35	1568784.0
36	2628714.0
37	5975326.0
38	1.0581672E7
39	6838551.0
40	452755.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34428556480404	20.484002969702555	13.920879350850923	24.250832114642478
2	14.14057381031274	21.71843507637268	41.56227344184042	22.578717671474163
3	17.220867890589382	27.568113815345292	30.927184986060833	24.283833308004493
4	11.82020718159781	18.866231342818775	40.08749349131885	29.226067984264564
5	12.470537898186729	38.7035833806229	36.15266412440444	12.67321459678593
6	28.34071295283604	39.371130604628455	19.667499819376314	12.620656623159196
7	25.288403087294803	33.175526362338594	24.46068258314295	17.07538796722365
8	24.077264523596707	36.7931812025593	21.89719671979113	17.23235755405286
9	25.776581748214745	16.372629064421506	21.385377311586183	36.46541187577756
10-11	23.64969111713062	26.840302820608734	29.858311454639384	19.65169460762126
12-13	24.813497560325423	24.601986431996174	29.633024237298063	20.951491770380347
14-15	21.990319998461487	25.68105980935397	27.171008922503226	25.15761126968131
16-17	21.456424846843568	28.737424854317656	27.703374486468174	22.102775812370602
18-19	21.496551333251947	28.045218828711445	29.35444627005857	21.103783567978034
20-21	22.493099554717507	27.222144719698566	29.02253033398515	21.26222539159878
22-23	22.15533867776609	27.195311340386663	28.927450189727832	21.721899792119416
24-25	21.810388838582366	27.361161908258484	29.025359005475256	21.803090247683894
26-27	21.849466593046234	27.455903376890216	28.89432629436875	21.800303735694797
28-29	21.81556276591558	27.46022882913564	28.92448435903021	21.799724045918573
30-31	22.102172530796857	27.315432616383482	28.79689084434787	21.785504008471786
32-33	21.910739191727775	27.53211855232729	28.75543040726779	21.80171184867715
34-35	22.02985168500064	27.498458202258274	28.772142332171054	21.699547780570033
36-37	22.012282133661273	27.432616829986774	28.75538656041514	21.79971447593681
38-39	21.89401909402171	27.501398873446885	28.766864590547513	21.837717441983887
40-41	22.164744051368583	27.315531702676505	28.726257809593434	21.793466436361477
42-43	21.965080358406226	27.42610385732795	28.548982774474702	22.059833009791124
44-45	22.21216347105387	27.527109276216006	28.296119835223493	21.96460741750663
46-47	22.16651330396292	27.52680865427465	28.14398198517597	22.162696056586462
48-49	22.10958612319934	27.72866108819287	28.00458351089294	22.157169277714853
50-51	22.05180511951152	27.85429343451974	27.77485821241246	22.31904323355628
52-53	22.131730370686288	27.666316573211986	27.742384268616654	22.459568787485075
54-55	22.24440601685916	27.43473515126383	28.005817062012227	22.315041769864784
56-57	22.113908902197917	27.26546585989853	28.10114727615388	22.519477961749672
58-59	21.977734169002986	27.19878262925809	28.11610306943636	22.707380132302564
60-61	22.15610083654719	27.354045655904603	28.313471805938427	22.176381701609778
62-63	21.79664165474652	27.578565612712836	28.27865457878091	22.346138153759735
64-65	21.678354060910358	27.796277765309263	27.99595573450745	22.529412439272928
66-67	21.752007894928397	28.23937867487188	27.628474562695487	22.380138867504236
68-69	21.833472944524125	28.209310543477134	27.499366498878658	22.457850013120087
70-71	21.84917403589758	27.71243016839731	27.53765636065139	22.90073943505372
72-73	21.979581432608285	27.396138763935728	27.505968574432732	23.118311229023256
74-75	21.9824122676201	27.503515876657268	27.669210450326354	22.84486140539628
76-77	22.288052465341245	27.394038333422806	27.18232357323331	23.135585628002637
78-79	22.388706197283856	27.473478948157894	26.8416263487744	23.296188505783853
80-81	22.334936244996012	27.74947584949811	26.92893921465985	22.98664869084603
82-83	22.294162487047515	27.541835089479022	27.048535237209375	23.115467186264084
84-85	22.34527579068716	27.833967202859572	26.99049972562187	22.830257280831397
86-87	22.647128006234052	27.78089821747961	27.07539101499188	22.49658276129446
88-89	22.497017838003817	27.854385628202976	27.263568895114897	22.38502763867831
90-91	22.48591810560838	27.55036670282364	27.23286220498703	22.73085298658095
92-93	22.47309376453904	27.26603545702777	27.330781371822148	22.93008940661104
94-95	21.906270576389893	27.590058320518317	27.837602920056113	22.666068183035676
96-97	22.03461247729177	27.537404968998352	27.94681166962719	22.481170884082687
98-99	23.165388230916857	29.479114805379915	28.234465013175154	19.121031950528078
100	22.136249958683962	30.52982801068469	22.020051784536435	25.31387024609491
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4819.0
1	4950.0
2	8156.5
3	11536.0
4	15238.0
5	26505.0
6	35806.0
7	41112.0
8	43097.0
9	39374.5
10	32838.5
11	26022.0
12	26898.5
13	37023.0
14	64009.0
15	104282.5
16	137248.0
17	154414.0
18	156417.0
19	145730.0
20	128221.5
21	111695.5
22	105011.0
23	115186.5
24	142777.5
25	186565.5
26	244912.5
27	310736.5
28	380438.0
29	455813.5
30	528213.0
31	598134.0
32	672618.0
33	745634.0
34	821984.5
35	894837.0
36	965630.0
37	1036677.5
38	1084539.0
39	1117166.5
40	1160022.0
41	1196363.5
42	1214474.0
43	1234467.0
44	1258513.0
45	1287541.0
46	1304397.0
47	1304284.5
48	1298188.5
49	1274589.0
50	1244854.0
51	1209031.0
52	1154004.0
53	1090703.5
54	1026208.0
55	948503.5
56	865311.5
57	779145.0
58	692320.5
59	606466.0
60	505791.0
61	406265.0
62	317860.0
63	241300.5
64	177979.0
65	128858.0
66	94670.5
67	70767.0
68	52871.5
69	38356.0
70	28040.5
71	20459.0
72	14525.0
73	10047.5
74	6903.5
75	4798.0
76	3220.5
77	2129.5
78	1434.5
79	1003.5
80	706.5
81	500.5
82	378.0
83	278.5
84	204.5
85	153.5
86	122.0
87	96.0
88	64.0
89	39.0
90	24.5
91	18.5
92	14.5
93	9.5
94	5.0
95	2.5
96	3.0
97	3.5
98	4.5
99	7.0
100	14.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.06673733827951578
6	0.0
7	0.0
8	2.4290771748960478E-4
9	8.439486082010629E-4
10-11	4.671302259415477E-6
12-13	3.7370418075323815E-4
14-15	0.006158333478662737
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.013912840028315292
24-25	0.0
26-27	7.789532199176406E-6
28-29	0.00281323413916516
30-31	0.01387806571713407
32-33	0.0
34-35	3.288833330227213E-5
36-37	6.439774156177936E-5
38-39	6.776681307240105E-5
40-41	0.0
42-43	0.005038324766881598
44-45	0.021607633569393953
46-47	0.0
48-49	0.0028243870331740527
50-51	3.079827540797517E-4
52-53	0.0
54-55	1.1505263732119855E-5
56-57	0.015215285681670956
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	1.6912288912225595E-5
68-69	0.015753215935000068
70-71	0.023249516937016136
72-73	0.0
74-75	0.013189129391017799
76-77	0.0
78-79	0.017496279245302306
80-81	1.0387186309659789E-4
82-83	0.014319394584239397
84-85	0.0
86-87	0.015190939048187415
88-89	0.03075785037601602
90-91	2.250394620761717E-5
92-93	1.8008639521593478E-4
94-95	0.01410753399251436
96-97	1.947052326330331E-6
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	513.0
20-21	2372.0
22-23	4639.0
24-25	6776.0
26-27	11377.0
28-29	22619.0
30-31	52019.0
32-33	64998.0
34-35	84872.0
36-37	125206.0
38-39	101175.0
40-41	169865.0
42-43	193604.0
44-45	244481.0
46-47	239192.0
48-49	216051.0
50-51	115702.0
52-53	27858.0
54-55	27315.0
56-57	26202.0
58-59	119807.0
60-61	218948.0
62-63	204933.0
64-65	210339.0
66-67	227607.0
68-69	238428.0
70-71	245345.0
72-73	224711.0
74-75	220023.0
76-77	230800.0
78-79	249173.0
80-81	259657.0
82-83	246777.0
84-85	236990.0
86-87	243494.0
88-89	263588.0
90-91	285471.0
92-93	288561.0
94-95	334344.0
96-97	1067057.0
98-99	2157888.0
100-101	2.2600183E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.16620396360157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.49879009845678	63.5145329121816
2	9.561074834096349	14.373394021818884
3	2.8221726609257134	6.363960175549272
4	1.2131768699525245	3.6475960020310083
5	0.6572786139103597	2.4702569177049716
6	0.39427549615725227	1.7781715417203736
7	0.2292140361770396	1.2060404296222595
8	0.15478845618467588	0.9307888535030683
9	0.10457446382863404	0.7074418929774643
>10	0.3544747824612322	4.098205764601563
>50	0.0068281250428608034	0.35161812465048964
>100	0.0032282490454599326	0.42372915234066466
>500	5.6124638879756885E-5	0.0308504279429015
>1k	6.718912218560159E-5	0.10341378335536244
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.294142560670874E-5	0.0	9.342604518830954E-6	0.0
3	0.0	5.6055627112985724E-5	0.0	9.342604518830954E-6	0.0
4	0.0	1.8062368736406512E-4	0.0	1.2456806025107937E-5	0.0
5	0.0	1.8685209037661908E-4	0.0	1.5571007531384923E-5	0.0
6	8.408344066947858E-5	1.8685209037661908E-4	0.0	1.5571007531384923E-5	6.228403012553969E-6
7	8.408344066947858E-5	1.8996629188289607E-4	0.0	1.5571007531384923E-5	6.228403012553969E-6
8	8.408344066947858E-5	2.05537299414281E-4	0.0	1.8685209037661907E-5	1.2456806025107937E-5
9	8.408344066947858E-5	3.6747577774068417E-4	0.0	2.179941054393889E-5	1.5571007531384923E-5
10-11	9.342604518830952E-5	3.6747577774068417E-4	0.0	3.4256216569046834E-5	1.5571007531384923E-5
12-13	1.0588285121341747E-4	3.6747577774068417E-4	0.0	3.737041807532382E-5	1.7128108284523413E-5
14-15	1.0899705271969447E-4	5.574420696235802E-4	0.0	4.204172033473929E-5	2.179941054393889E-5
16-17	1.7906658661092663E-4	5.792414801675191E-4	0.0	4.359882108787778E-5	2.8027813556492862E-5
18-19	1.8062368736406512E-4	6.446397117993359E-4	0.0	4.9827224100431757E-5	3.7370418075323814E-5
20-21	1.821807881172036E-4	7.05366641171737E-4	0.0	5.916982861926271E-5	3.7370418075323814E-5
22-23	1.8996629188289607E-4	8.190349961508469E-4	0.0	7.474083615064764E-5	4.671302259415477E-5
24-25	2.008659971548655E-4	9.218036458579874E-4	0.0	9.031184368203256E-5	5.9169828619262705E-5
26-27	2.05537299414281E-4	9.731879707115577E-4	0.0	1.3391066476991034E-4	5.9169828619262705E-5
28-29	2.1020860167369647E-4	9.872018774898042E-4	0.0	4.0796039732228495E-4	5.9169828619262705E-5
30-31	2.1332280317997343E-4	0.001141354852050515	0.0	0.001502602226778645	5.9169828619262705E-5
32-33	2.179941054393889E-4	0.0011460261543099304	0.0	0.0038039971399173363	6.228403012553969E-5
34-35	2.2733670995821986E-4	0.0013468921514647957	0.0	0.006440168714980804	6.851243313809367E-5
36-37	2.3356511297077385E-4	0.0015524294508790768	0.0	0.012654557820756526	6.851243313809367E-5
38-39	2.3356511297077385E-4	0.001591356969707539	0.0	0.12236320558463527	7.006953389123216E-5
40-41	2.5225032200843574E-4	0.0017439528435151113	0.0	0.2371277594939547	7.162663464437065E-5
42-43	2.5692162426785124E-4	0.0018140223774063434	0.0	0.2526442684989798	8.09692391632016E-5
44-45	2.678213295398207E-4	0.0020024315685361013	0.0	0.2726763696881065	9.186894443517104E-5
46-47	2.7716393405865164E-4	0.0021176570242683497	0.0	0.2926555294516265	1.01211548954002E-4
48-49	2.802781355649286E-4	0.002120771225774627	0.0	0.313259086617155	1.0899705271969445E-4
50-51	3.00520445355729E-4	0.0023683502455236465	0.0	0.3348514027609265	1.1211125422597144E-4
52-53	3.1142015062769843E-4	0.002382364152301893	0.0	0.7204020060440424	1.2612516100421787E-4
54-55	3.129772513808369E-4	0.0023963780590801394	0.0	1.2140294155017477	1.3858196702932582E-4
56-57	3.176485536402524E-4	0.0024228487718834938	0.0	1.751581080104737	1.416961685356028E-4
58-59	3.176485536402524E-4	0.0024306342756491863	0.0	2.320956769900371	1.4481037004187978E-4
60-61	3.3789086343105287E-4	0.0024680046937245103	0.0	2.4722991776016663	1.4636747079501826E-4
62-63	3.9238938979090006E-4	0.0025302887238500497	0.0	2.4782255030681113	1.5259587380757225E-4
64-65	4.0017489355659254E-4	0.002868179587281103	0.0	2.483695598013887	1.5259587380757225E-4
66-67	4.4688791615074726E-4	0.002897764501590734	0.0	2.488721919245018	1.5259587380757225E-4
68-69	4.5934472217585523E-4	0.0029834050430133513	0.0	2.493481976247362	1.5259587380757225E-4
70-71	4.7647283046037866E-4	0.0030472461738920292	0.0	2.497413655649037	1.619384783264032E-4
72-73	5.263000545608104E-4	0.0030628171814234143	0.0	2.500395503591297	2.02423097908004E-4
74-75	5.294142560670874E-4	0.0031515719243523084	0.0	2.5026984556051888	2.02423097908004E-4
76-77	5.434281628453339E-4	0.0033321956117163733	0.0	2.5051104046718002	2.05537299414281E-4
78-79	5.589991703767187E-4	0.0034271787576578214	0.0	2.506750031764855	2.05537299414281E-4
80-81	5.621133718829957E-4	0.00342873585841096	0.0	2.5073214877412573	2.1020860167369647E-4
82-83	5.683417748955496E-4	0.0034302929591640986	0.0	2.507712320030295	2.2889381071135838E-4
84-85	5.730130771549652E-4	0.0034427497651892064	0.0	2.5078384451912994	2.3045091146449686E-4
86-87	5.823556816737961E-4	0.003508147996821023	0.0	2.5078898295161527	2.3045091146449686E-4
88	5.948124876989041E-4	0.0037339276060261045	0.0	2.5078976150199184	2.3356511297077385E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	38665	0.0	51.018013	1
GTATCAA	70140	0.0	39.08007	1
ATCTCGT	26080	0.0	37.655746	94
TCAACGC	85605	0.0	31.304337	4
ATCAACG	86040	0.0	31.151157	3
CAACGCA	87260	0.0	30.6956	5
AACGCAG	90275	0.0	29.772905	6
TATCAAC	91335	0.0	29.63734	2
TATCTCG	31225	0.0	29.378315	94
ACGCAGA	104000	0.0	25.806072	7
CGCAGAG	105495	0.0	25.423813	8
GTACATG	102295	0.0	23.388536	1
TACATGG	101825	0.0	22.997702	2
TATGCCG	14810	0.0	22.772831	86-87
TCGTATG	15795	0.0	22.157501	84-85
ACATGGG	103005	0.0	22.10109	3
CGCTATC	45580	0.0	21.818972	94
GCCGTCT	16545	0.0	21.69261	90-91
TCTCGTA	16030	0.0	21.386719	82-83
CTCGTAT	15510	0.0	21.262018	82-83
>>END_MODULE