FastQCFastQC Report
Fri 27 May 2016
SRR2139915_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139915_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34552957
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA456550.052.499161
GTATCAA841600.038.5942341
TCAACGC1036900.030.6597144
ATCAACG1039400.030.5735993
CAACGCA1053300.030.166065
AACGCAG1084350.029.3709416
TATCAAC1096700.029.2847442
ACGCAGA1237250.025.720687
CGCAGAG1253050.025.3861778
GTACATG1167950.022.0962161
TACATGG1172850.021.6823022
GCAGAGT1488150.021.3578039
ACATGGG1183150.020.9430473
GAGTACT836000.019.64719812-13
AGAGTAC1346850.019.3917110-11
CATGGGG817750.018.9847354
CAGAGTA1447750.018.9309110-11
AGTACTT916700.017.26566112-13
GTACTTT951350.017.2294114-15
GTGGTAT205100.016.1960681
Produced by FastQC (version 0.11.5)