##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139915_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34552957
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73524072049752	34.0	31.0	34.0	31.0	34.0
2	32.7883712239158	34.0	31.0	34.0	31.0	34.0
3	32.87714646245761	34.0	31.0	34.0	31.0	34.0
4	36.29903790868029	37.0	37.0	37.0	35.0	37.0
5	36.24269685514904	37.0	37.0	37.0	35.0	37.0
6	36.310971793239	37.0	37.0	37.0	35.0	37.0
7	36.28385941035379	37.0	37.0	37.0	35.0	37.0
8	36.28991877598204	37.0	37.0	37.0	35.0	37.0
9	38.078708169607594	39.0	39.0	39.0	35.0	39.0
10-11	38.055035492331385	39.0	39.0	39.0	35.0	39.0
12-13	38.04464975313111	39.0	39.0	39.0	35.0	39.0
14-15	39.54297088090029	41.0	40.0	41.0	37.0	41.0
16-17	39.55937778349911	41.0	40.0	41.0	37.0	41.0
18-19	39.60460619911633	41.0	40.0	41.0	37.0	41.0
20-21	39.62065337098838	41.0	40.0	41.0	37.0	41.0
22-23	39.57873564760662	41.0	40.0	41.0	37.0	41.0
24-25	39.55429256661601	41.0	40.0	41.0	36.0	41.0
26-27	39.48119586146241	41.0	39.0	41.0	36.0	41.0
28-29	39.42930083252277	41.0	39.0	41.0	36.0	41.0
30-31	39.352056583040465	41.0	39.0	41.0	36.0	41.0
32-33	39.27783399918087	41.0	39.0	41.0	35.0	41.0
34-35	39.22714531844289	41.0	39.0	41.0	35.0	41.0
36-37	39.18263004904657	41.0	39.0	41.0	35.0	41.0
38-39	39.13973884888087	41.0	39.0	41.0	35.0	41.0
40-41	39.10330053331294	41.0	39.0	41.0	35.0	41.0
42-43	39.03139408331842	41.0	39.0	41.0	35.0	41.0
44-45	38.65610361218741	40.5	38.5	41.0	34.5	41.0
46-47	38.7141796202357	40.0	38.0	41.0	34.5	41.0
48-49	38.79852044657769	40.0	38.0	41.0	35.0	41.0
50-51	38.76279385606078	40.0	38.0	41.0	35.0	41.0
52-53	38.67183952749862	40.0	38.0	41.0	34.5	41.0
54-55	38.55418454228918	40.0	38.0	41.0	34.0	41.0
56-57	38.40057040872733	40.0	37.5	41.0	34.0	41.0
58-59	38.24885247472453	40.0	37.0	41.0	34.0	41.0
60-61	38.06818421781878	40.0	36.5	41.0	34.0	41.0
62-63	37.84349549505821	39.0	36.0	41.0	33.5	41.0
64-65	37.59709629720054	39.0	35.5	41.0	33.0	41.0
66-67	37.337308929173325	39.0	35.0	41.0	33.0	41.0
68-69	36.97671196794016	38.0	35.0	40.0	32.5	41.0
70-71	36.668636555571524	37.0	35.0	40.0	32.0	41.0
72-73	36.386762350393475	37.0	35.0	39.5	32.0	41.0
74-75	36.0521575589123	36.0	35.0	39.0	32.0	41.0
76-77	35.717045875501285	36.0	35.0	39.0	32.0	40.5
78-79	35.38344771171897	35.5	35.0	37.5	31.5	39.5
80-81	35.072726451942955	35.0	34.0	37.0	31.0	39.0
82-83	34.78262694235384	35.0	34.0	37.0	31.0	39.0
84-85	34.52460289740722	35.0	34.0	36.0	31.0	37.5
86-87	34.30297919495014	35.0	34.0	36.0	31.0	37.0
88-89	34.24893782541412	35.0	34.0	36.0	31.0	37.0
90-91	34.21494999618308	35.0	34.0	35.0	31.0	36.5
92-93	34.133965670958546	35.0	34.0	35.0	31.0	36.0
94-95	34.174934906893206	35.0	34.0	35.0	32.0	36.0
96-97	34.22134959516494	35.0	34.0	35.0	32.0	36.0
98-99	34.32087508939968	35.0	35.0	35.0	32.0	36.0
100	34.1039169917127	35.0	34.0	35.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	32.0
20	108.0
21	535.0
22	2581.0
23	8947.0
24	24491.0
25	53141.0
26	97923.0
27	158933.0
28	231914.0
29	316014.0
30	410073.0
31	519534.0
32	660701.0
33	852116.0
34	1181830.0
35	1730918.0
36	2855430.0
37	6314282.0
38	1.0854392E7
39	7717796.0
40	561261.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.43680669645727	20.50852840178049	13.990903875462815	24.063761026299428
2	14.19357828043487	21.75672258672391	41.64465576708818	22.405043365753038
3	17.264615008203204	27.68924234183488	30.968324939599235	24.077817710362677
4	11.777706897849583	18.96044092550458	40.1364635738701	29.125388602775732
5	12.37253563094468	38.76154575013355	36.14271352546137	12.723205093460404
6	28.087306681161905	39.55281453914349	19.692323293777722	12.667555485916878
7	25.16813828697787	33.35057546594348	24.44286027386889	17.038425973209762
8	24.004581035310277	36.94650357446243	21.8073956654506	17.241519724776698
9	25.707910431506527	16.459711429873046	21.43149636521272	36.40088177340771
10-11	23.57139966135168	26.997579903044183	29.78205959203865	19.64896084356549
12-13	24.813429839110956	24.73778188691348	29.625629098453587	20.823159175521972
14-15	21.926251666096107	25.811695453434574	27.28719410139751	24.974858779071806
16-17	21.304626692297276	28.914040555197634	27.814095621396458	21.967237131108632
18-19	21.35793327326515	28.10068759093469	29.57874632842567	20.96263280737449
20-21	22.36170647513913	27.256407718406827	29.30627561198888	21.07561019446516
22-23	22.05420460753595	27.21195496516453	29.19895120802533	21.534889219274184
24-25	21.684134484530627	27.366837608084698	29.301113149357327	21.647914758027344
26-27	21.685358804576556	27.46308955410121	29.205211228528267	21.646340412793965
28-29	21.638342196782908	27.462175952013613	29.252046274799326	21.647435576404156
30-31	21.903632709747413	27.349560125519545	29.118540732775166	21.628266431957872
32-33	21.733448446433037	27.54966533685628	29.054841234344615	21.662044982366073
34-35	21.869361501634287	27.53819186591746	29.089437583408078	21.50300904904017
36-37	21.853185846943408	27.4623907791079	28.98088825209581	21.703535121852884
38-39	21.734666491504683	27.559667096659567	29.042556547905118	21.66310986393063
40-41	21.95430046640691	27.410991013860365	28.917328818710352	21.71737970102238
42-43	21.838999133943027	27.52031682085876	28.75538652990776	21.885297515290453
44-45	22.106535379289763	27.6179798815334	28.487702528628407	21.787782210548436
46-47	22.077463839386397	27.682039329610035	28.170776458517093	22.06972037248648
48-49	22.02623748410102	27.78387080219747	28.117383369667092	22.072508344034414
50-51	22.02153881576909	27.921008737754814	27.958294696094356	22.099157750381735
52-53	22.15056385320204	27.8211747473593	27.903128775916862	22.125132623521797
54-55	22.0762006793976	27.753491110362898	27.982298364553344	22.188009845686153
56-57	22.10480538994674	27.815631258743995	27.968243965997104	22.111319385312164
58-59	22.0914175425356	27.867558740865395	27.943657034092784	22.09736668250622
60-61	22.163275639275412	27.84579759215816	27.934430738238973	22.056496030327455
62-63	22.124495082484685	27.91648588957814	27.872896444457133	22.086122583480037
64-65	22.10112364383999	27.871668947004842	27.84779661867121	22.179410790483956
66-67	22.141824285112065	27.896968524232168	27.76422927693526	22.196977913720502
68-69	22.16370301717026	27.872735790396785	27.788289644500285	22.175271547932667
70-71	22.16230735403799	27.643529195903216	27.869187712362887	22.324975737695908
72-73	22.142744864926044	27.669571951057936	27.84757717153954	22.340106012476483
74-75	22.032800897385922	27.83338580846942	27.839161128265662	22.294652165878997
76-77	22.163810693997902	27.789957377619583	27.732500384952264	22.313731543430247
78-79	22.11652854082765	27.806880542482347	27.722025662091234	22.354565254598768
80-81	22.14814950926566	27.822538476130877	27.721248648022044	22.308063366581422
82-83	22.182681288491636	27.736389757566137	27.838666196047523	22.2422627578947
84-85	22.239255266369405	27.773816791008304	27.748792349990286	22.238135592632005
86-87	22.080852035988595	27.82899530128481	27.867085899898527	22.22306676282807
88-89	22.14486507872848	27.775366305450543	27.792812059033565	22.28695655678741
90-91	22.209651483219055	27.769930776400614	27.794885690473514	22.225532049906818
92-93	22.229327929381775	27.738446679914023	27.789160709726847	22.24306468097736
94-95	22.0477956237185	27.836340052820773	27.853865669847554	22.261998653613173
96-97	22.16675187751944	27.9402318282684	27.87402137229516	22.018994921917002
98-99	23.234945557176832	29.88801868871721	28.073531273468866	18.80350448063709
100	22.15906619212123	30.793634862059633	21.972874753814573	25.074424192004564
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11255.0
1	10827.5
2	12292.5
3	14371.5
4	18446.0
5	31586.0
6	42751.0
7	49764.0
8	53226.5
9	49165.0
10	40127.0
11	30655.5
12	31528.5
13	44518.5
14	77878.5
15	127129.5
16	168013.0
17	190226.5
18	194170.5
19	182203.0
20	161872.0
21	143033.0
22	136171.0
23	149359.0
24	182561.0
25	235137.0
26	305258.5
27	382197.0
28	462397.5
29	551508.5
30	636465.5
31	712986.5
32	797011.5
33	878716.5
34	955221.5
35	1027780.0
36	1100070.5
37	1168771.0
38	1217260.5
39	1249403.5
40	1280134.0
41	1307193.0
42	1324186.0
43	1347397.0
44	1370684.5
45	1390040.0
46	1397454.0
47	1393212.5
48	1384226.0
49	1355384.0
50	1321606.5
51	1282299.5
52	1224493.0
53	1156917.5
54	1089848.0
55	1015152.0
56	935235.5
57	851592.5
58	760669.0
59	667560.5
60	561327.0
61	452024.5
62	354298.0
63	272435.5
64	204235.5
65	149096.0
66	109028.0
67	81772.0
68	61579.5
69	44391.5
70	31773.0
71	23178.0
72	16716.5
73	11829.5
74	8527.0
75	6035.5
76	4176.5
77	2835.5
78	1947.0
79	1400.5
80	990.0
81	702.5
82	526.0
83	372.5
84	262.0
85	198.0
86	149.0
87	104.0
88	68.0
89	42.5
90	30.5
91	27.0
92	20.0
93	14.0
94	8.5
95	6.0
96	7.5
97	6.5
98	6.5
99	7.5
100	10.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.06575124670227211
6	0.0
7	0.0
8	1.9969347341242024E-4
9	9.145382260626782E-4
10-11	7.235270775812328E-6
12-13	3.993869468248405E-4
14-15	0.006161556592681777
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.014094866163174525
24-25	0.0
26-27	1.7374967345920743E-5
28-29	0.0027929817178920712
30-31	0.013743508541531824
32-33	0.0
34-35	4.369868463463355E-5
36-37	9.499953990992057E-5
38-39	8.078517431655999E-5
40-41	0.0
42-43	0.004931825396485447
44-45	0.021374384142968144
46-47	3.0259736872221664E-6
48-49	0.0027658258586584225
50-51	3.324008514817145E-4
52-53	0.0
54-55	1.5605776852840723E-5
56-57	0.015269315959797792
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	2.782312310367004E-5
68-69	0.016039783418341377
70-71	0.023474083721542964
72-73	0.0
74-75	0.013415678152688609
76-77	0.0
78-79	0.017806901524500204
80-81	9.074108505920987E-5
82-83	0.014659148700659074
84-85	0.0
86-87	0.015535415842199423
88-89	0.03129959986061559
90-91	3.486603441945557E-5
92-93	1.3181620187469373E-4
94-95	0.01426501224842579
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	771.0
20-21	2836.0
22-23	5746.0
24-25	8236.0
26-27	14530.0
28-29	27866.0
30-31	63919.0
32-33	79484.0
34-35	104154.0
36-37	155777.0
38-39	203038.0
40-41	211194.0
42-43	244497.0
44-45	306970.0
46-47	297969.0
48-49	272315.0
50-51	239653.0
52-53	218586.0
54-55	229410.0
56-57	244190.0
58-59	256213.0
60-61	261061.0
62-63	243239.0
64-65	245922.0
66-67	266057.0
68-69	277698.0
70-71	283568.0
72-73	260254.0
74-75	251699.0
76-77	264935.0
78-79	284585.0
80-81	294620.0
82-83	277583.0
84-85	264291.0
86-87	270335.0
88-89	293742.0
90-91	315354.0
92-93	316951.0
94-95	359015.0
96-97	1056266.0
98-99	2084021.0
100-101	2.3194407E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.13204190807348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.01319939551877	67.20386899818152
2	8.980050828484853	14.032594153356168
3	2.493240876467345	5.844060019412053
4	1.0554920656586875	3.2987100123073447
5	0.49871856949865273	1.9482950086201594
6	0.2921978333187511	1.3698008014985357
7	0.17954955802963105	0.9820001514783041
8	0.12068450818661103	0.7543461641033222
9	0.0790303998472262	0.5557325861587752
>10	0.27946272665352667	3.3551338213485145
>50	0.0062296710802921375	0.321801626951635
>100	0.002080752902034928	0.27090309305523763
>500	3.6038968680226347E-5	0.02030665496765509
>1k	2.6775384871788746E-5	0.042446908560706084
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.788216620649862E-6	0.0
2	2.894108310324931E-6	2.3152866482599448E-5	0.0	1.7364649861949588E-5	0.0
3	2.894108310324931E-6	2.3152866482599448E-5	0.0	2.0258758172274516E-5	0.0
4	2.894108310324931E-6	5.498805789617369E-5	0.0	4.6305732965198896E-5	0.0
5	2.894108310324931E-6	5.788216620649862E-5	0.0	4.6305732965198896E-5	0.0
6	7.814092437877315E-5	5.788216620649862E-5	0.0	5.788216620649862E-5	2.894108310324931E-6
7	7.814092437877315E-5	5.788216620649862E-5	0.0	6.0776274516823555E-5	2.894108310324931E-6
8	7.814092437877315E-5	6.945859944779835E-5	0.0	6.656449113747342E-5	8.682324930974794E-6
9	7.814092437877315E-5	1.4181130720592163E-4	0.0	9.261146593039779E-5	1.7364649861949588E-5
10-11	7.814092437877315E-5	1.4181130720592163E-4	0.0	1.4325836136108407E-4	1.7364649861949588E-5
12-13	7.814092437877315E-5	1.4181130720592163E-4	0.0	1.7075239030917092E-4	1.881170401711205E-5
14-15	7.95879785339356E-5	2.0692874418823257E-4	0.0	1.9824641925725778E-4	2.4599920637761914E-5
16-17	1.2444665734397204E-4	2.185051774295323E-4	0.0	2.315286648259945E-4	3.183519141357424E-5
18-19	1.302348739646219E-4	2.488933146879441E-4	0.0	2.51787422998269E-4	4.051751634454903E-5
20-21	1.3747014474043423E-4	2.8796377687733066E-4	0.0	3.0098726427379286E-4	4.919984127552383E-5
22-23	1.4904657798173396E-4	3.472929972389917E-4	0.0	3.35716563997692E-4	5.788216620649862E-5
24-25	1.5338774044722136E-4	3.9215167604902815E-4	0.0	3.7044586372159116E-4	6.367038282714849E-5
26-27	1.5338774044722136E-4	4.167515966867901E-4	0.0	4.167515966867901E-4	6.656449113747342E-5
28-29	1.5338774044722136E-4	4.167515966867901E-4	0.0	6.511743698231094E-4	6.656449113747342E-5
30-31	1.5483479460238383E-4	4.5148089641068926E-4	0.0	0.0015367715127825384	6.945859944779835E-5
32-33	1.6062301122303366E-4	4.543750047210142E-4	0.0	0.003558306167544503	7.524681606844821E-5
34-35	1.7364649861949586E-4	5.310688749446249E-4	0.0	0.006108015588940767	8.3929140999423E-5
36-37	1.765406069298208E-4	6.12103907633723E-4	0.0	0.01041734286301459	8.3929140999423E-5
38-39	1.7943471524014572E-4	6.439390990472971E-4	0.0	0.021464154283524852	9.261146593039779E-5
40-41	1.7943471524014572E-4	6.974801027883084E-4	0.0	0.03393486699271498	9.261146593039779E-5
42-43	1.7943471524014572E-4	7.235270775812327E-4	0.0	0.04196312344555633	9.261146593039779E-5
44-45	1.8377587770563312E-4	8.146914893564681E-4	0.0	0.05113165857266572	9.695262839588519E-5
46-47	1.8811704017112051E-4	8.855971429594289E-4	0.0	0.06061420445144536	9.984673670621012E-5
48-49	1.8956409432628298E-4	8.870441971145914E-4	0.0	0.07002873878493236	1.0129379086137259E-4
50-51	2.054816900330701E-4	0.0010158320169240509	0.0	0.07966033124169372	1.0418789917169752E-4
52-53	2.1126990665371997E-4	0.0010216202335447007	0.0	0.09887142220563062	1.0708200748202245E-4
54-55	2.1271696080888243E-4	0.0010404319375618128	0.0	0.11939643834245503	1.1142316994750985E-4
56-57	2.141640149640449E-4	0.00105056131664795	0.0	0.14021375941862227	1.1287022410267231E-4
58-59	2.141640149640449E-4	0.0010563495332686	0.0	0.15582023848204946	1.1287022410267231E-4
60-61	2.2429339405018216E-4	0.0010795023997511994	0.0	0.16463569239529918	1.1721138656815971E-4
62-63	2.662579645498937E-4	0.0010954199954579865	0.0	0.17425570841881927	1.2155254903364711E-4
64-65	2.7204618117054354E-4	0.0012271019235777709	0.0	0.1802841360292261	1.2155254903364711E-4
66-67	2.95199047653143E-4	0.0012444665734397205	0.0	0.18573084786925761	1.258937114991345E-4
68-69	3.0098726427379286E-4	0.0012661723857671575	0.0	0.19128753582508148	1.302348739646219E-4
70-71	3.1401075167025505E-4	0.0012980075771807315	0.0	0.1963840605595637	1.345760364301093E-4
72-73	3.5018710554931664E-4	0.0013168192811978435	0.0	0.20032149491576076	1.4470541551624656E-4
74-75	3.530812138596416E-4	0.001358783851697555	0.0	0.20319968563037888	1.475995238265715E-4
76-77	3.559753221699665E-4	0.0014282424511453534	0.0	0.2059679002292047	1.5049363213689643E-4
78-79	3.5886943048029144E-4	0.0014528423717831153	0.0	0.2077058122695548	1.5049363213689643E-4
80-81	3.5886943048029144E-4	0.0014528423717831153	0.0	0.20834541020613662	1.591759570678712E-4
82-83	3.603164846354539E-4	0.0014571835342486027	0.0	0.20871296196154787	1.765406069298208E-4
84-85	3.6176353879061637E-4	0.00146731291333474	0.0	0.2088634555936848	1.7798766108498325E-4
86-87	3.6176353879061637E-4	0.0015034892672138016	0.0	0.20891989070573613	1.8088176939530818E-4
88	3.7044586372159116E-4	0.0016120183288509866	0.0	0.20892278481404644	1.8232882355047065E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	45655	0.0	52.49916	1
GTATCAA	84160	0.0	38.594234	1
TCAACGC	103690	0.0	30.659714	4
ATCAACG	103940	0.0	30.573599	3
CAACGCA	105330	0.0	30.16606	5
AACGCAG	108435	0.0	29.370941	6
TATCAAC	109670	0.0	29.284744	2
ACGCAGA	123725	0.0	25.72068	7
CGCAGAG	125305	0.0	25.386177	8
GTACATG	116795	0.0	22.096216	1
TACATGG	117285	0.0	21.682302	2
GCAGAGT	148815	0.0	21.357803	9
ACATGGG	118315	0.0	20.943047	3
GAGTACT	83600	0.0	19.647198	12-13
AGAGTAC	134685	0.0	19.39171	10-11
CATGGGG	81775	0.0	18.984735	4
CAGAGTA	144775	0.0	18.93091	10-11
AGTACTT	91670	0.0	17.265661	12-13
GTACTTT	95135	0.0	17.22941	14-15
GTGGTAT	20510	0.0	16.196068	1
>>END_MODULE