FastQCFastQC Report
Fri 27 May 2016
SRR2139914_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139914_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35578963
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA475300.052.4506151
GTATCAA870500.038.663651
ATCAACG1076650.030.6358783
TCAACGC1077300.030.6013774
CAACGCA1099700.029.9937425
TATCAAC1131900.029.4452172
AACGCAG1135050.029.1392566
ACGCAGA1300950.025.3539437
CGCAGAG1320650.024.9758458
GTACATG1175800.022.3030571
TACATGG1172050.021.8666152
ACATGGG1177150.021.1673243
GCAGAGT1556750.021.1211879
GAGTACT886200.019.64106212-13
AGAGTAC1401900.019.27746410-11
CATGGGG810000.019.2314954
CAGAGTA1518700.018.71187210-11
AGTACTT964750.017.3782212-13
GTACTTT1012300.017.22463214-15
GTGGTAT201350.016.3255961
Produced by FastQC (version 0.11.5)