##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139914_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	35578963
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73842461344363	34.0	31.0	34.0	31.0	34.0
2	32.79533372009746	34.0	31.0	34.0	31.0	34.0
3	32.88180529601158	34.0	31.0	34.0	31.0	34.0
4	36.30286883291118	37.0	37.0	37.0	35.0	37.0
5	36.24730788246976	37.0	37.0	37.0	35.0	37.0
6	36.313660350359285	37.0	37.0	37.0	35.0	37.0
7	36.286657792696204	37.0	37.0	37.0	35.0	37.0
8	36.293808619436156	37.0	37.0	37.0	35.0	37.0
9	38.08207510151434	39.0	39.0	39.0	35.0	39.0
10-11	38.0590460997978	39.0	39.0	39.0	35.0	39.0
12-13	38.04817588415941	39.0	39.0	39.0	35.0	39.0
14-15	39.54758834595601	41.0	40.0	41.0	37.0	41.0
16-17	39.56366381448498	41.0	40.0	41.0	37.0	41.0
18-19	39.60926236101935	41.0	40.0	41.0	37.0	41.0
20-21	39.62496770507625	41.0	40.0	41.0	37.0	41.0
22-23	39.58364328076637	41.0	40.0	41.0	37.0	41.0
24-25	39.55955899714765	41.0	40.0	41.0	36.0	41.0
26-27	39.487253002632215	41.0	39.0	41.0	36.0	41.0
28-29	39.434508662678255	41.0	39.0	41.0	36.0	41.0
30-31	39.35625803698567	41.0	39.0	41.0	36.0	41.0
32-33	39.281301655751605	41.0	39.0	41.0	35.0	41.0
34-35	39.2316388879872	41.0	39.0	41.0	35.0	41.0
36-37	39.18761107801872	41.0	39.0	41.0	35.0	41.0
38-39	39.14555591462869	41.0	39.0	41.0	35.0	41.0
40-41	39.111721649180126	41.0	39.0	41.0	35.0	41.0
42-43	39.04119251954418	41.0	39.0	41.0	35.0	41.0
44-45	38.664727322428455	40.5	38.5	41.0	34.5	41.0
46-47	38.7263649571518	40.0	38.0	41.0	34.5	41.0
48-49	38.81261218599904	40.0	38.0	41.0	35.0	41.0
50-51	38.779267990402126	40.0	38.0	41.0	35.0	41.0
52-53	38.691803737143246	40.0	38.0	41.0	35.0	41.0
54-55	38.57705550342715	40.0	38.0	41.0	34.0	41.0
56-57	38.42792563223466	40.0	37.5	41.0	34.0	41.0
58-59	38.28012000483783	40.0	37.0	41.0	34.0	41.0
60-61	38.10185013409601	40.0	37.0	41.0	34.0	41.0
62-63	37.878891787660585	39.5	36.0	41.0	33.5	41.0
64-65	37.63418543163044	39.0	36.0	41.0	33.0	41.0
66-67	37.375413710787214	39.0	35.0	41.0	33.0	41.0
68-69	37.01766745461224	38.0	35.0	40.5	32.5	41.0
70-71	36.710175178980464	37.0	35.0	40.0	32.0	41.0
72-73	36.42670966079605	37.0	35.0	39.5	32.0	41.0
74-75	36.08966906579275	36.5	35.0	39.0	32.0	41.0
76-77	35.7511435845269	36.0	35.0	39.0	32.0	40.5
78-79	35.413092886752054	35.5	35.0	37.5	31.5	39.5
80-81	35.09996700735226	35.0	34.0	37.0	31.0	39.0
82-83	34.80728031522839	35.0	34.0	37.0	31.0	39.0
84-85	34.54693929114603	35.0	34.0	36.0	31.0	37.5
86-87	34.32134133225357	35.0	34.0	36.0	31.0	37.0
88-89	34.264524017166224	35.0	34.0	36.0	31.0	37.0
90-91	34.22539734016219	35.0	34.0	35.0	31.0	36.5
92-93	34.14354680650059	35.0	34.0	35.0	31.0	36.0
94-95	34.18240265411103	35.0	34.0	35.0	32.0	36.0
96-97	34.22660575920844	35.0	34.0	35.0	32.0	36.0
98-99	34.32431324321893	35.0	35.0	35.0	32.0	36.0
100	34.10963150170931	35.0	34.0	35.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	6.0
19	27.0
20	101.0
21	543.0
22	2533.0
23	9024.0
24	24656.0
25	53990.0
26	99677.0
27	163388.0
28	238079.0
29	324381.0
30	421508.0
31	535214.0
32	680667.0
33	877905.0
34	1217001.0
35	1779466.0
36	2926626.0
37	6487478.0
38	1.1311465E7
39	7869748.0
40	555480.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.23172448842874	20.650773323550773	14.063169856861766	24.054332331158722
2	14.11775548376719	21.884499556662178	41.67180757910229	22.325937380468343
3	17.14229276440688	27.769016202074244	31.140865460300233	23.947825573218644
4	11.726260824409076	19.11104885209836	40.264540593833495	28.898149729659067
5	12.298265773308865	38.82655775582496	36.24327965078991	12.631896820076275
6	27.945257988547894	39.69767752927481	19.75365611414813	12.603408368029164
7	24.987945826301907	33.46570837379381	24.582251034129353	16.964094765774934
8	23.889611040076467	37.028904560356644	21.921286232586723	17.160198166980166
9	25.636914850383473	16.500243390299975	21.567191251766474	36.29565050755008
10-11	23.453503883938602	27.071046284909784	29.91772722087078	19.557722610280834
12-13	24.739260820800833	24.815213351129888	29.73505445370328	20.710471374365998
14-15	21.854503469006954	25.87212570725962	27.434833005553738	24.83853781817969
16-17	21.19538166415924	28.971154949063582	27.95538335392181	21.878080032855372
18-19	21.2492547913777	28.177735253273124	29.72811911353347	20.84489084181571
20-21	22.23540552208903	27.31538648776879	29.461487825005676	20.9877201651365
22-23	21.93733617042667	27.307462877573062	29.32520616260935	21.429994789390914
24-25	21.587391378771063	27.48339071516297	29.412411498362495	21.516806407703477
26-27	21.610725896702135	27.574668624424536	29.29266316192069	21.521942316952643
28-29	21.57298773977046	27.571943974763947	29.34498836223682	21.510079923228773
30-31	21.872944624734654	27.431317317478875	29.194444291874944	21.501293765911534
32-33	21.70745715396338	27.597643506778528	29.150862941378374	21.54403639787972
34-35	21.78244587961688	27.648208358712168	29.16527893372117	21.404066827949784
36-37	21.79617651119321	27.542109664274932	29.094228206878892	21.567485617652963
38-39	21.679120188552513	27.63588624691155	29.15363946049801	21.531354104037923
40-41	21.881239221555298	27.503455899434236	29.049663002085595	21.56564187692487
42-43	21.797376989990706	27.60852076502518	28.83643342158395	21.757668823400163
44-45	22.049783968219522	27.723252438694306	28.55457105686906	21.67239253621711
46-47	22.0311764236979	27.796066190224977	28.22456798575667	21.94818940032045
48-49	22.00562303165998	27.859676642472326	28.161593812368707	21.973106513498987
50-51	21.97331792132523	27.995497351464984	28.00833309311537	22.022851634094422
52-53	22.08278722094773	27.915964807193582	27.973278737806652	22.027969234052033
54-55	22.05754492779778	27.85389683712803	28.01402483352598	22.07453340154821
56-57	22.068378309954383	27.918578192011083	28.024563435454848	21.988480062579686
58-59	22.045988249997745	27.99101051451202	28.007048883994628	21.955952351495604
60-61	22.13241082668253	27.979290809710538	27.968680238376788	21.919618125230144
62-63	22.093557799662552	28.04426931337024	27.900255138088752	21.961917748878452
64-65	22.070680567258936	28.005201630173186	27.894394682963863	22.029723119604018
66-67	22.11993141534914	28.029295555089845	27.79242031287323	22.058352716687786
68-69	22.106680188993757	27.970954822297532	27.85011336453122	22.07225162417749
70-71	22.11357025309745	27.732854618475233	27.941823812572526	22.21175131585479
72-73	22.137875768864244	27.80474947711043	27.898712642273406	22.158662111751916
74-75	22.011418753903406	27.947903953828256	27.878645823552144	22.16203146871619
76-77	22.131790331696603	27.871572837785756	27.8200724630396	22.17656436747804
78-79	22.0790396837637	27.911174395253784	27.79995070122413	22.209835219758386
80-81	22.12368529886181	27.91483026739269	27.816236485193425	22.145247948552075
82-83	22.133115001651962	27.880470978397586	27.903795372788892	22.08261864716156
84-85	22.221170639540567	27.90225088187888	27.78164248093485	22.094935997645706
86-87	22.02507103223761	27.921288768786944	27.92640127660846	22.12723892236699
88-89	22.13864391723251	27.856919286931465	27.833387310888497	22.17104948494753
90-91	22.206789973472834	27.88334988936117	27.84754006466675	22.06232007249925
92-93	22.1826108264228	27.89022029368013	27.82403300060821	22.103135879288857
94-95	22.040791877564466	27.919120740005983	27.87704950642874	22.16303787600081
96-97	22.12602707637746	28.036227532304064	27.933298746432524	21.90444664488595
98-99	23.182465670405776	29.999837248754236	28.120845718144967	18.69685136269503
100	22.110565260857467	30.94661610740471	21.99687616164231	24.945942470095513
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6183.0
1	6293.0
2	10445.5
3	14846.5
4	19421.0
5	33379.5
6	45257.0
7	52482.0
8	55907.5
9	51742.5
10	42668.0
11	32951.0
12	33674.5
13	47037.0
14	82330.5
15	135128.5
16	178603.5
17	201823.5
18	205618.5
19	192191.0
20	169639.5
21	149140.5
22	140709.0
23	151880.0
24	186188.5
25	241292.5
26	312852.5
27	391558.0
28	474741.0
29	564977.0
30	649035.5
31	728934.0
32	819610.5
33	903899.5
34	982149.0
35	1059640.0
36	1136396.0
37	1211277.5
38	1263363.5
39	1295351.0
40	1328224.5
41	1356553.0
42	1372367.0
43	1389118.5
44	1411638.0
45	1436663.5
46	1441913.0
47	1435220.0
48	1422618.5
49	1386427.0
50	1345362.5
51	1296975.0
52	1231568.0
53	1160374.0
54	1086877.5
55	1004218.0
56	919907.0
57	833118.0
58	741931.0
59	649783.5
60	541978.0
61	436091.0
62	342994.5
63	262300.0
64	196383.0
65	142233.0
66	102517.0
67	76507.0
68	57725.5
69	41826.0
70	30010.0
71	21392.0
72	14989.0
73	10395.5
74	7239.5
75	4892.0
76	3327.0
77	2273.5
78	1534.5
79	1078.5
80	749.0
81	521.5
82	387.5
83	280.5
84	211.0
85	158.5
86	125.5
87	98.5
88	68.5
89	44.5
90	30.0
91	21.0
92	18.5
93	11.5
94	4.5
95	5.0
96	5.0
97	6.0
98	8.0
99	9.0
100	12.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0661036691822637
6	0.0
7	0.0
8	2.220413225646852E-4
9	8.600587937315654E-4
10-11	5.6212993054350685E-6
12-13	4.539199189138818E-4
14-15	0.006214346382158469
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.013969194265335843
24-25	0.0
26-27	2.249714071886998E-5
28-29	0.002819082034302489
30-31	0.01422355705773366
32-33	0.0
34-35	2.827658978162937E-5
36-37	6.0980419357522214E-5
38-39	5.983294129935437E-5
40-41	0.0
42-43	0.00495534976260647
44-45	0.021835627941800766
46-47	0.0
48-49	0.0028247484446394908
50-51	3.3003535347626313E-4
52-53	0.0
54-55	9.034018726767985E-6
56-57	0.014631620643502404
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	1.8860241055884308E-5
68-69	0.01569615016388194
70-71	0.022816336100370017
72-73	0.0
74-75	0.013186417775278167
76-77	0.0
78-79	0.016668552124938996
80-81	8.501037409931926E-5
82-83	0.01423363024818416
84-85	0.0
86-87	0.015081594149006356
88-89	0.029942864295752533
90-91	1.748014177234024E-5
92-93	1.520000681525887E-4
94-95	0.013531985042981975
96-97	1.8164116754655765E-6
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	662.0
20-21	2500.0
22-23	5057.0
24-25	7867.0
26-27	13798.0
28-29	27141.0
30-31	59903.0
32-33	74848.0
34-35	97490.0
36-37	144832.0
38-39	192187.0
40-41	198322.0
42-43	233697.0
44-45	294084.0
46-47	282728.0
48-49	253106.0
50-51	225591.0
52-53	204660.0
54-55	216616.0
56-57	229698.0
58-59	237708.0
60-61	242340.0
62-63	226937.0
64-65	232616.0
66-67	251092.0
68-69	263029.0
70-71	270458.0
72-73	247852.0
74-75	243144.0
76-77	255314.0
78-79	275309.0
80-81	287454.0
82-83	272851.0
84-85	263662.0
86-87	269919.0
88-89	292623.0
90-91	313426.0
92-93	317075.0
94-95	365369.0
96-97	1096149.0
98-99	2182056.0
100-101	2.4407793E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.24883038167437
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.60060679377133	66.12546754780443
2	9.019546019079053	13.934987610950882
3	2.619786194752664	6.071262581841021
4	1.102116556138288	3.405488596238468
5	0.5783758659959922	2.233942958458921
6	0.34084264327871977	1.579781732248762
7	0.21347058893130952	1.1543247321081604
8	0.1237321422207362	0.764653061373669
9	0.08760465648792372	0.6090621524712422
>10	0.3063419842530642	3.5016390987576944
>50	0.005711028939165974	0.2925410392006498
>100	0.0017704185869914187	0.22673322542229898
>500	5.2182599082016535E-5	0.02823411848558436
>1k	4.292496578579504E-5	0.07188154463823601
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.810649652717534E-5	0.0	0.0	0.0
3	0.0	3.091714617989288E-5	0.0	0.0	0.0
4	0.0	7.307689097065589E-5	0.0	1.1242598610870137E-5	5.6212993054350685E-6
5	0.0	9.275143853967863E-5	0.0	1.405324826358767E-5	5.6212993054350685E-6
6	0.0	9.275143853967863E-5	0.0	1.405324826358767E-5	1.1242598610870137E-5
7	0.0	1.0118338749783124E-4	0.0	4.778104409619808E-5	1.1242598610870137E-5
8	8.431948958152602E-6	1.1804728541413643E-4	0.0	5.059169374891562E-5	1.405324826358767E-5
9	8.431948958152602E-6	1.8269222742663973E-4	0.0	7.588754062337342E-5	1.405324826358767E-5
10-11	8.431948958152602E-6	1.8550287707935726E-4	0.0	8.713013923424356E-5	1.405324826358767E-5
12-13	8.431948958152602E-6	1.9112417638479233E-4	0.0	9.83727378451137E-5	1.405324826358767E-5
14-15	9.83727378451137E-6	2.8528093975082973E-4	0.0	1.292898840250066E-4	2.6701171700816577E-5
16-17	1.1242598610870137E-5	2.951182135353411E-4	0.0	1.3350585850408286E-4	2.810649652717534E-5
18-19	1.2647923437228905E-5	3.246300348888752E-4	0.0	1.4193780746223547E-4	2.810649652717534E-5
20-21	1.6863897916305204E-5	3.696004293323558E-4	0.0	1.5458573089946438E-4	3.091714617989287E-5
22-23	2.529584687445781E-5	3.9630160103317234E-4	0.0	1.6863897916305205E-4	3.5133120658969175E-5
24-25	2.6701171700816577E-5	4.328400465185003E-4	0.0	2.1360937360653261E-4	3.653844548532794E-5
26-27	2.810649652717534E-5	4.52514594087523E-4	0.0	2.8387561492447094E-4	4.215974479076301E-5
28-29	2.810649652717534E-5	4.5391991891388173E-4	0.0	4.1035484929676E-4	4.215974479076301E-5
30-31	3.091714617989288E-5	4.974849885310036E-4	0.0	8.727067171687944E-4	4.215974479076301E-5
32-33	3.091714617989288E-5	4.988903133573623E-4	0.0	0.0018086530515237333	4.778104409619808E-5
34-35	3.091714617989288E-5	5.480766822799192E-4	0.0	0.0028935638174727015	5.059169374891562E-5
36-37	3.091714617989288E-5	6.085056498133462E-4	0.0	0.004640382576636649	5.059169374891562E-5
38-39	3.091714617989288E-5	6.33801496687804E-4	0.0	0.007651993679523487	5.480766822799192E-5
40-41	3.091714617989288E-5	6.829878656103609E-4	0.0	0.011260867833612801	6.323961718614453E-5
42-43	3.232247100625164E-5	7.096890373111774E-4	0.0	0.014656132614095582	6.605026683886205E-5
44-45	3.372779583261041E-5	7.799552786291158E-4	0.0	0.018371811454988164	6.886091649157958E-5
46-47	3.5133120658969175E-5	8.291416475516726E-4	0.0	0.022769072836664746	7.167156614429712E-5
48-49	3.934909513804548E-5	8.347629468571077E-4	0.0	0.02721973656174296	8.150883992880849E-5
50-51	3.934909513804548E-5	9.696741301875493E-4	0.0	0.032010488894799995	8.150883992880849E-5
52-53	3.934909513804548E-5	9.83727378451137E-4	0.0	0.03672254303758095	9.134611371331986E-5
54-55	3.934909513804548E-5	0.0010357243970264113	0.0	0.04136854691352303	1.0258871232419E-4
56-57	3.934909513804548E-5	0.0010596149190745105	0.0	0.04604125196116593	1.0539936197690754E-4
58-59	3.934909513804548E-5	0.0010778841418171742	0.0	0.050803897797695785	1.0680468680326631E-4
60-61	3.934909513804548E-5	0.0010835054411226096	0.0	0.055612919353495494	1.152366357614189E-4
62-63	3.934909513804548E-5	0.0011059906383443497	0.0	0.06018725166329328	1.2085793506685397E-4
64-65	3.934909513804548E-5	0.0012057687010158223	0.0	0.06459575564358074	1.2226325989321275E-4
66-67	3.934909513804548E-5	0.0012170112996266924	0.0	0.06879486622474072	1.2507390954593028E-4
68-69	3.934909513804548E-5	0.0012409018216747913	0.0	0.07306424304721866	1.2647923437228905E-4
70-71	3.934909513804548E-5	0.0012633870188965316	0.0	0.07697526203897512	1.292898840250066E-4
72-73	3.934909513804548E-5	0.001271818967854684	0.0	0.08006557133213804	1.5177508124674684E-4
74-75	3.934909513804548E-5	0.001298520139555501	0.0	0.08251645782930772	1.5177508124674684E-4
76-77	3.934909513804548E-5	0.001368786380873439	0.0	0.08497577627543557	1.5177508124674684E-4
78-79	3.934909513804548E-5	0.0014011088518796908	0.0	0.08656379332922098	1.5177508124674684E-4
80-81	3.934909513804548E-5	0.001417972749795996	0.0	0.08713997650802807	1.5458573089946438E-4
82-83	3.934909513804548E-5	0.0014264046987541485	0.0	0.08743368939673705	1.6020703020489945E-4
84-85	3.934909513804548E-5	0.0014502952208022477	0.0	0.08752222486079766	1.6020703020489945E-4
86-87	3.934909513804548E-5	0.0014980762648984457	0.0	0.08756438460558842	1.6020703020489945E-4
88	3.934909513804548E-5	0.0015739638055218192	0.0	0.08756578993041478	1.6301767985761698E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	47530	0.0	52.450615	1
GTATCAA	87050	0.0	38.66365	1
ATCAACG	107665	0.0	30.635878	3
TCAACGC	107730	0.0	30.601377	4
CAACGCA	109970	0.0	29.993742	5
TATCAAC	113190	0.0	29.445217	2
AACGCAG	113505	0.0	29.139256	6
ACGCAGA	130095	0.0	25.353943	7
CGCAGAG	132065	0.0	24.975845	8
GTACATG	117580	0.0	22.303057	1
TACATGG	117205	0.0	21.866615	2
ACATGGG	117715	0.0	21.167324	3
GCAGAGT	155675	0.0	21.121187	9
GAGTACT	88620	0.0	19.641062	12-13
AGAGTAC	140190	0.0	19.277464	10-11
CATGGGG	81000	0.0	19.231495	4
CAGAGTA	151870	0.0	18.711872	10-11
AGTACTT	96475	0.0	17.37822	12-13
GTACTTT	101230	0.0	17.224632	14-15
GTGGTAT	20135	0.0	16.325596	1
>>END_MODULE