FastQCFastQC Report
Fri 27 May 2016
SRR2139913_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139913_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32026301
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA422000.049.5195581
GTATCAA757200.036.638221
ATCAACG915750.029.5804843
TCAACGC916250.029.5502474
CAACGCA929650.029.1196775
AACGCAG960950.028.2553426
TATCAAC972900.028.1391352
ACGCAGA1091250.024.8462457
CGCAGAG1110500.024.4117858
GTACATG963000.022.022041
TACATGG973900.021.205352
GCAGAGT1322500.020.5139969
ACATGGG983800.020.4976253
GAGTACT761100.019.21912-13
AGAGTAC1172350.019.14972910-11
CATGGGG713400.018.6172524
CAGAGTA1270450.018.57365410-11
AGTACTT828950.017.00485812-13
GTACTTT859750.016.90407814-15
GTGGTAT194300.014.5351821
Produced by FastQC (version 0.11.5)