##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139913_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32026301
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75905528396801	34.0	31.0	34.0	31.0	34.0
2	32.815829339766715	34.0	31.0	34.0	31.0	34.0
3	32.903111945397626	34.0	31.0	34.0	31.0	34.0
4	36.31757567007192	37.0	37.0	37.0	35.0	37.0
5	36.25851040368352	37.0	37.0	37.0	35.0	37.0
6	36.323911962233794	37.0	37.0	37.0	35.0	37.0
7	36.29747300507792	37.0	37.0	37.0	35.0	37.0
8	36.304408554706335	37.0	37.0	37.0	35.0	37.0
9	38.097304118886534	39.0	39.0	39.0	35.0	39.0
10-11	38.0754303470763	39.0	39.0	39.0	35.0	39.0
12-13	38.0634313965887	39.0	39.0	39.0	35.0	39.0
14-15	39.571457456170165	41.0	40.0	41.0	37.0	41.0
16-17	39.587169011494645	41.0	40.0	41.0	37.0	41.0
18-19	39.630009675485155	41.0	40.0	41.0	37.0	41.0
20-21	39.643550438586445	41.0	40.0	41.0	37.0	41.0
22-23	39.60163975093232	41.0	40.0	41.0	37.0	41.0
24-25	39.57758506798643	41.0	40.0	41.0	36.5	41.0
26-27	39.505159138813056	41.0	40.0	41.0	36.0	41.0
28-29	39.453956722521426	41.0	39.0	41.0	36.0	41.0
30-31	39.37836190244845	41.0	39.0	41.0	36.0	41.0
32-33	39.30530660445993	41.0	39.0	41.0	35.5	41.0
34-35	39.25634273650666	41.0	39.0	41.0	35.0	41.0
36-37	39.21108793798841	41.0	39.0	41.0	35.0	41.0
38-39	39.16885822500044	41.0	39.0	41.0	35.0	41.0
40-41	39.13261722311955	41.0	39.0	41.0	35.0	41.0
42-43	39.060416415672165	41.0	39.0	41.0	35.0	41.0
44-45	38.68344113169325	40.5	38.5	41.0	34.5	41.0
46-47	38.74241031149165	40.0	38.0	41.0	34.5	41.0
48-49	38.823677873066615	40.0	38.0	41.0	35.0	41.0
50-51	38.78597873301692	40.0	38.0	41.0	35.0	41.0
52-53	38.6934631671522	40.0	38.0	41.0	35.0	41.0
54-55	38.57336405557902	40.0	38.0	41.0	34.0	41.0
56-57	38.417989153547495	40.0	37.5	41.0	34.0	41.0
58-59	38.26360327021815	40.0	37.0	41.0	34.0	41.0
60-61	38.07968451633768	40.0	36.5	41.0	34.0	41.0
62-63	37.8507582685474	39.0	36.0	41.0	33.5	41.0
64-65	37.59816289171039	39.0	35.5	41.0	33.0	41.0
66-67	37.333584013842525	39.0	35.0	41.0	33.0	41.0
68-69	36.96830368784343	38.0	35.0	40.0	32.5	41.0
70-71	36.655475379839814	37.0	35.0	40.0	32.0	41.0
72-73	36.369341394531276	37.0	35.0	39.0	32.0	41.0
74-75	36.031149080466804	36.0	35.0	39.0	32.0	41.0
76-77	35.69427780479948	36.0	35.0	39.0	32.0	40.0
78-79	35.36057165914195	35.0	35.0	37.0	31.5	39.5
80-81	35.05279596861239	35.0	34.0	37.0	31.0	39.0
82-83	34.76804737013765	35.0	34.0	36.5	31.0	39.0
84-85	34.515838311117136	35.0	34.0	36.0	31.0	37.0
86-87	34.2970064780076	35.0	34.0	36.0	31.0	37.0
88-89	34.246830259011546	35.0	34.0	35.5	31.0	37.0
90-91	34.21453336764668	35.0	34.0	35.0	31.0	36.0
92-93	34.136697275190386	35.0	34.0	35.0	31.0	36.0
94-95	34.180207521873434	35.0	34.0	35.0	32.0	36.0
96-97	34.228795985661066	35.0	34.0	35.0	32.0	36.0
98-99	34.32845142304306	35.0	35.0	35.0	32.5	36.0
100	34.11610252199531	35.0	34.0	35.0	32.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	5.0
19	24.0
20	108.0
21	514.0
22	2261.0
23	8151.0
24	21928.0
25	47803.0
26	89193.0
27	144528.0
28	211867.0
29	288366.0
30	374292.0
31	477273.0
32	604750.0
33	780165.0
34	1082381.0
35	1594590.0
36	2650938.0
37	5915857.0
38	1.0227777E7
39	6989155.0
40	514373.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.38082009533352	20.408950755817852	13.979794294695475	24.230434854153156
2	14.265044220998234	21.70455464088719	41.37730111260742	22.65310002550716
3	17.27126401516054	27.412791130639786	31.011417771911905	24.304527082287773
4	11.95025301236006	18.853838287475035	39.93883027577865	29.257078424386258
5	12.531699177129013	38.596973064081006	36.09449640231798	12.776831356472007
6	28.398955595902255	39.32104116550956	19.53415413163075	12.745849106957435
7	25.26669252249893	33.21805412370289	24.408728938131194	17.106524415666986
8	24.13028127399887	36.7476080945487	21.781482655865524	17.340627975586898
9	25.692743592578626	16.396429691388196	21.386986278379144	36.52384043765404
10-11	23.702714932939095	26.8314640491927	29.750605732679524	19.71521528518868
12-13	24.868747502529377	24.57446301112817	29.54649485291747	21.01029463342498
14-15	22.040228112427076	25.63440230451276	27.183480829937984	25.14188875312218
16-17	21.490806884004492	28.709712682710375	27.722964322354933	22.0765161109302
18-19	21.551639697634766	27.995182771809958	29.36135677985416	21.091820750701118
20-21	22.470209609037003	27.189741958817297	29.091563546758113	21.248484885387587
22-23	22.190476937086963	27.072547958858884	28.992513922688335	21.74446118136582
24-25	21.887523436111703	27.24198148323662	29.046298748545173	21.824196332106503
26-27	21.914448782436814	27.328431856867567	28.953674460145567	21.803444900550055
28-29	21.85245067273734	27.368963745597757	28.97418054312165	21.80440503854325
30-31	22.15194621839925	27.23086969828766	28.837121259712717	21.780062823600378
32-33	21.949351433778205	27.413725876737296	28.800914279810456	21.83600840967404
34-35	22.05171802055842	27.4079540530034	28.82252494984032	21.71780297659786
36-37	22.063939343573548	27.34060797827157	28.771240483545018	21.82421219460986
38-39	21.93729805708018	27.453284956637624	28.796374075705472	21.813042910576723
40-41	22.16660915357318	27.29109029136242	28.67527820714605	21.86702234791835
42-43	22.048971982152935	27.41759661070029	28.473872991548372	22.05955841559841
44-45	22.26846661824399	27.491859878591953	28.26153946962995	21.978134033534104
46-47	22.265470168324306	27.554416395206495	27.976974505944007	22.203138930525196
48-49	22.23078462627249	27.641980424945373	27.895965936957072	22.231269011825066
50-51	22.192403501470196	27.781626261939742	27.768780946472756	22.257189290117307
52-53	22.354753515920944	27.653328409935934	27.701814967493466	22.290103106649653
54-55	22.294947107753046	27.574823209289878	27.792916257829887	22.33731342512719
56-57	22.320965899701477	27.680645428482904	27.745894085810757	22.25249458600486
58-59	22.296293774541002	27.734297064274756	27.75057883216034	22.218830329023906
60-61	22.357990517364108	27.711984673990266	27.723559904423677	22.206464904221956
62-63	22.321813493007426	27.73972940614355	27.696200053186224	22.2422570476628
64-65	22.31915810786454	27.69063560621245	27.67244765306388	22.31775863285913
66-67	22.360094082488224	27.752320268336394	27.58608700984773	22.301498639327647
68-69	22.340191244299753	27.7121437609433	27.63080942927008	22.316855565486865
70-71	22.32834950855012	27.477809368367385	27.709074021305756	22.48476710177674
72-73	22.36661509795391	27.533584826212287	27.618460432713487	22.481339643120315
74-75	22.207383870591162	27.726031426643672	27.62423518717952	22.442349515585647
76-77	22.36571345633288	27.638489797386335	27.56092407155726	22.43487267472353
78-79	22.31151572576214	27.676595178793562	27.525477886411764	22.486411209032536
80-81	22.336108036870307	27.68590232159034	27.51979263538813	22.458197006151227
82-83	22.369613478386793	27.625826207375447	27.64302037173256	22.3615399425052
84-85	22.431165568450993	27.638829749227494	27.542649508913186	22.38735517340832
86-87	22.25354162924415	27.7089237583859	27.63666955761505	22.400865054754902
88-89	22.33146299058075	27.632875640499062	27.579111773472892	22.4565495954473
90-91	22.39607002591848	27.638433767853837	27.59255012587158	22.3729460803561
92-93	22.43838379961111	27.645691836910004	27.566255873841445	22.349668489637445
94-95	22.254748975194822	27.681192558586936	27.623900718837813	22.440157747380425
96-97	22.357719868090733	27.801203203399005	27.66222163818201	22.17885529032825
98-99	23.435009769996316	29.754336449004455	27.83791417969474	18.972739601304493
100	22.36058452032912	30.670934085335094	21.72277603783644	25.24570535649935
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6648.0
1	6597.0
2	9525.0
3	12813.0
4	16515.0
5	27945.5
6	37737.0
7	43925.0
8	46930.5
9	43311.5
10	35980.0
11	28294.0
12	28820.0
13	39644.0
14	68404.0
15	111249.5
16	146332.5
17	165238.5
18	169068.0
19	158734.5
20	140382.5
21	123901.5
22	117571.5
23	128500.0
24	158442.0
25	205967.0
26	268164.0
27	336447.0
28	406668.5
29	483308.0
30	557685.5
31	625809.5
32	700419.0
33	773541.0
34	841614.5
35	907632.0
36	977935.5
37	1044398.5
38	1090390.5
39	1125547.0
40	1165953.5
41	1197719.5
42	1215055.5
43	1239439.0
44	1267092.5
45	1298415.5
46	1318660.0
47	1322378.0
48	1318302.5
49	1290828.0
50	1254634.5
51	1216167.5
52	1162047.5
53	1098686.5
54	1034797.0
55	962261.5
56	884451.5
57	804947.5
58	721374.0
59	632547.5
60	529287.5
61	427622.0
62	336980.0
63	258873.5
64	194973.5
65	143636.5
66	105831.5
67	79804.5
68	60602.5
69	44952.0
70	32695.0
71	23734.5
72	17454.5
73	12425.0
74	8671.0
75	5903.5
76	3945.0
77	2607.0
78	1768.5
79	1244.0
80	867.5
81	627.5
82	460.0
83	339.5
84	253.5
85	172.0
86	109.0
87	69.5
88	54.0
89	45.0
90	34.0
91	25.5
92	17.0
93	8.5
94	5.0
95	5.5
96	6.5
97	7.0
98	7.5
99	9.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.06721662923233002
6	0.0
7	0.0
8	1.7485628452689557E-4
9	8.555468207208819E-4
10-11	1.0928517782930973E-5
12-13	4.6368139736149985E-4
14-15	0.006258918255967181
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.014221358600675775
24-25	0.0
26-27	2.8117268190579904E-5
28-29	0.002777136195588154
30-31	0.014140890894586644
32-33	0.0
34-35	3.613270808457404E-5
36-37	8.983318025706884E-5
38-39	5.3842178661049216E-5
40-41	0.0
42-43	0.004948423977440956
44-45	0.021673119282988314
46-47	1.6284678854652286E-6
48-49	0.002769951838303905
50-51	3.143485660361463E-4
52-53	0.0
54-55	1.3415020523472212E-5
56-57	0.015046932751153826
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	1.92955858698565E-5
68-69	0.01571917053579006
70-71	0.02311719337465342
72-73	0.0
74-75	0.013180692133716834
76-77	0.0
78-79	0.01655739940318017
80-81	8.203769400842549E-5
82-83	0.014429339488746827
84-85	0.0
86-87	0.015279771874771026
88-89	0.02989346927767427
90-91	2.7416594606717176E-5
92-93	1.544904888821811E-4
94-95	0.01327894350753453
96-97	6.110153729227437E-6
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	652.0
20-21	2371.0
22-23	4732.0
24-25	7234.0
26-27	12733.0
28-29	24950.0
30-31	55688.0
32-33	70040.0
34-35	91622.0
36-37	137685.0
38-39	182215.0
40-41	184627.0
42-43	214386.0
44-45	266896.0
46-47	258715.0
48-49	235995.0
50-51	212597.0
52-53	195868.0
54-55	205781.0
56-57	217330.0
58-59	224449.0
60-61	228419.0
62-63	213248.0
64-65	216845.0
66-67	232901.0
68-69	244531.0
70-71	249091.0
72-73	230763.0
74-75	227493.0
76-77	237786.0
78-79	255040.0
80-81	265736.0
82-83	252722.0
84-85	243516.0
86-87	249264.0
88-89	268355.0
90-91	287683.0
92-93	290019.0
94-95	332943.0
96-97	993546.0
98-99	1958041.0
100-101	2.1741793E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.71134124821013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.97879434623384	66.81527427549858
2	8.859744537072377	13.770052621847938
3	2.5810175415152337	6.017230048089201
4	1.0457555443611966	3.2506826388024277
5	0.5140743120093817	1.9974702143749954
6	0.3154875016873606	1.4710174141903047
7	0.1891276233714836	1.0288152895499028
8	0.1223389555900766	0.7605699460648054
9	0.08692206664032551	0.6079347344417397
>10	0.2979312658131649	3.5718432225011427
>50	0.006338859848435188	0.32928202422055064
>100	0.0023681195416530142	0.2949764559602554
>500	5.975283291043485E-5	0.03198877363805383
>1k	3.957348252028357E-5	0.052862340820033854
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.244867304531984E-6
2	0.0	3.122433652265992E-5	0.0	0.0	6.244867304531984E-6
3	0.0	3.122433652265992E-5	0.0	1.561216826132996E-5	6.244867304531984E-6
4	3.122433652265992E-6	1.1553004513384172E-4	0.0	1.561216826132996E-5	6.244867304531984E-6
5	3.122433652265992E-6	1.1553004513384172E-4	0.0	1.8734601913595952E-5	6.244867304531984E-6
6	7.493840765438381E-5	1.1553004513384172E-4	0.0	2.1857035565861946E-5	9.367300956797976E-6
7	7.493840765438381E-5	1.186524787861077E-4	0.0	3.122433652265992E-5	9.367300956797976E-6
8	7.493840765438381E-5	1.3114221339517168E-4	0.0	3.7469203827191904E-5	9.367300956797976E-6
9	7.493840765438381E-5	2.2481522296315145E-4	0.0	4.371407113172389E-5	1.561216826132996E-5
10-11	8.742814226344778E-5	2.2481522296315145E-4	0.0	4.6836504783989886E-5	1.561216826132996E-5
12-13	9.679544322024575E-5	2.2793765661541744E-4	0.0	5.464258891465486E-5	1.561216826132996E-5
14-15	1.0147909369864474E-4	2.9975363061753523E-4	0.0	5.932623939305385E-5	2.6540686044260934E-5
16-17	1.608053330916986E-4	3.0443728109593425E-4	0.0	6.713232352371884E-5	3.7469203827191904E-5
18-19	1.6548898357009759E-4	3.3722283444472716E-4	0.0	8.586692543731479E-5	4.9958938436255874E-5
20-21	1.6861141722236358E-4	3.8249812240258405E-4	0.0	1.202136956122407E-4	5.308137208852187E-5
22-23	1.8422358548369355E-4	4.2465097670817494E-4	0.0	1.4050951435196963E-4	6.088745621918685E-5
24-25	1.920296696143585E-4	5.011506011886918E-4	0.0	1.592441162655656E-4	6.869354034985183E-5
26-27	1.967133200927575E-4	5.448646723204156E-4	0.0	2.0295818739728952E-4	7.025475717598483E-5
28-29	1.967133200927575E-4	5.479871059726816E-4	0.0	3.7937568875031806E-4	8.742814226344778E-5
30-31	1.967133200927575E-4	6.198030799747995E-4	0.0	9.726380826808566E-4	9.367300956797977E-5
32-33	1.998357537450235E-4	6.276091641054645E-4	0.0	0.0022184891099349875	9.679544322024577E-5
34-35	2.0920305470182148E-4	7.165985231950452E-4	0.0	0.003737553081762393	1.186524787861077E-4
36-37	2.2013157248475246E-4	8.08710315936892E-4	0.0	0.006057521285396024	1.186524787861077E-4
38-39	2.2325400613701845E-4	8.36812218807286E-4	0.0	0.009882502509421865	1.233361292645067E-4
40-41	2.3106009026768344E-4	9.117506264616698E-4	0.0	0.01452243891668913	1.248973460906397E-4
42-43	2.404273912244814E-4	9.336076620275317E-4	0.0	0.018896968463513783	1.2645856291677269E-4
44-45	2.4823347535514635E-4	0.0010257194547693784	0.0	0.023585302592391173	1.4363194800423565E-4
46-47	2.5603955948581136E-4	0.0011022190792498953	0.0	0.028988674027637475	1.545604657871666E-4
48-49	2.576007763119444E-4	0.0011100251633805601	0.0	0.0347339519478069	1.639277667439646E-4
50-51	2.7165172774714134E-4	0.001289565098385855	0.0	0.04099443142060021	1.6705020039623058E-4
52-53	2.8258024553007234E-4	0.0013020548329949187	0.0	0.0466101283442006	2.013969705711565E-4
54-55	2.857026791823383E-4	0.0013114221339517167	0.0	0.05221489675001806	2.2013157248475246E-4
56-57	2.872638960084713E-4	0.0013301567358653128	0.0	0.057711941194832334	2.2481522296315145E-4
58-59	2.903863296607373E-4	0.0013395240368221108	0.0	0.06324333240982155	2.3262130709381643E-4
60-61	3.028760642698013E-4	0.0013707483733447707	0.0	0.06905730387034081	2.419886080506144E-4
62-63	3.3410040079246117E-4	0.0013926054089106327	0.0	0.07454810344785057	2.576007763119444E-4
64-65	3.3410040079246117E-4	0.001570584127089794	0.0	0.07971104749187238	2.5916199313807736E-4
66-67	3.419064849231262E-4	0.00157370656074206	0.0	0.08493956264259178	2.5916199313807736E-4
68-69	3.4971256905379114E-4	0.00162054306552605	0.0	0.0904053827508834	2.6228442679034335E-4
70-71	3.590798700105891E-4	0.001637716450613513	0.0	0.0950562476759336	2.778965950516733E-4
72-73	3.809369055764511E-4	0.001643961317918045	0.0	0.0986470463760395	2.9194754648687027E-4
74-75	3.809369055764511E-4	0.0016892366058759017	0.0	0.10159774617743086	2.935087633130033E-4
76-77	3.8874298970711604E-4	0.0017829096154438817	0.0	0.1043017737202932	2.935087633130033E-4
78-79	3.99671507490047E-4	0.0018281849034017385	0.0	0.10621114189865385	2.935087633130033E-4
80-81	3.99671507490047E-4	0.0018313073370540044	0.0	0.10678410847384467	3.0131484744366825E-4
82-83	4.02793941142313E-4	0.0018359909875324035	0.0	0.10713382104289845	3.138045820527322E-4
84-85	4.05916374794579E-4	0.0018594092399243983	0.0	0.10724154500390164	3.153657988788652E-4
86-87	4.07477591620712E-4	0.0018968784437515902	0.0	0.10728682029185949	3.169270157049982E-4
88	4.1528367575137696E-4	0.002026459440320629	0.0	0.10729306515916402	3.184882325311312E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	42200	0.0	49.519558	1
GTATCAA	75720	0.0	36.63822	1
ATCAACG	91575	0.0	29.580484	3
TCAACGC	91625	0.0	29.550247	4
CAACGCA	92965	0.0	29.119677	5
AACGCAG	96095	0.0	28.255342	6
TATCAAC	97290	0.0	28.139135	2
ACGCAGA	109125	0.0	24.846245	7
CGCAGAG	111050	0.0	24.411785	8
GTACATG	96300	0.0	22.02204	1
TACATGG	97390	0.0	21.20535	2
GCAGAGT	132250	0.0	20.513996	9
ACATGGG	98380	0.0	20.497625	3
GAGTACT	76110	0.0	19.219	12-13
AGAGTAC	117235	0.0	19.149729	10-11
CATGGGG	71340	0.0	18.617252	4
CAGAGTA	127045	0.0	18.573654	10-11
AGTACTT	82895	0.0	17.004858	12-13
GTACTTT	85975	0.0	16.904078	14-15
GTGGTAT	19430	0.0	14.535182	1
>>END_MODULE