FastQCFastQC Report
Fri 27 May 2016
SRR2139912_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139912_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30179726
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA469050.054.9867551
GTATCAA916300.040.2563131
ATCAACG1143750.031.6984883
TCAACGC1143400.031.6748034
CAACGCA1163000.031.1336945
TATCAAC1177200.031.0173992
AACGCAG1195600.030.3531916
ACGCAGA1351000.026.818227
CGCAGAG1369500.026.459048
GCAGAGT1587250.022.7381529
GTACATG1275450.021.8675731
TACATGG1262550.021.7013572
ACATGGG1277850.020.8972873
GAGTACT865950.020.24416712-13
CTTATAC279400.020.0833281
AGAGTAC1462500.019.75307310-11
CAGAGTA1554550.019.60463110-11
CATGGGG816150.019.0722164
AGTACTT930500.017.987912-13
GTACTTT973500.017.9516114-15
Produced by FastQC (version 0.11.5)