##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139912_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30179726
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.637450883417564	34.0	31.0	34.0	31.0	34.0
2	32.683953260543184	34.0	31.0	34.0	31.0	34.0
3	32.77624336284564	34.0	31.0	34.0	31.0	34.0
4	36.235234673767415	37.0	37.0	37.0	35.0	37.0
5	36.19433420303418	37.0	37.0	37.0	35.0	37.0
6	36.27895892759265	37.0	37.0	37.0	35.0	37.0
7	36.22460250301808	37.0	37.0	37.0	35.0	37.0
8	36.24113442911973	37.0	37.0	37.0	35.0	37.0
9	38.04212397422031	39.0	39.0	39.0	35.0	39.0
10-11	38.02090284053607	39.0	38.5	39.0	35.0	39.0
12-13	38.02185198765555	39.0	39.0	39.0	35.0	39.0
14-15	39.48855587688238	41.0	39.0	41.0	36.0	41.0
16-17	39.506209963602714	41.0	39.0	41.0	36.0	41.0
18-19	39.57015393380311	41.0	39.5	41.0	36.5	41.0
20-21	39.593834731545336	41.0	40.0	41.0	37.0	41.0
22-23	39.5515505104535	41.0	40.0	41.0	36.5	41.0
24-25	39.52525096924764	41.0	39.5	41.0	36.0	41.0
26-27	39.447350510652186	41.0	39.0	41.0	36.0	41.0
28-29	39.38909895304343	41.0	39.0	41.0	36.0	41.0
30-31	39.30437568306018	41.0	39.0	41.0	36.0	41.0
32-33	39.21814977190259	41.0	39.0	41.0	35.0	41.0
34-35	39.16361738043669	41.0	39.0	41.0	35.0	41.0
36-37	39.11885457687909	41.0	39.0	41.0	35.0	41.0
38-39	39.06894795680637	41.0	39.0	41.0	35.0	41.0
40-41	39.04472698169495	41.0	39.0	41.0	35.0	41.0
42-43	38.97995201722566	41.0	39.0	41.0	35.0	41.0
44-45	38.60816020183139	40.5	38.0	41.0	34.5	41.0
46-47	38.66898221200941	40.0	38.0	41.0	34.5	41.0
48-49	38.757093018083566	40.0	38.0	41.0	35.0	41.0
50-51	38.71827933542944	40.0	38.0	41.0	35.0	41.0
52-53	38.63582093013203	40.0	38.0	41.0	34.0	41.0
54-55	38.52314562416851	40.0	38.0	41.0	34.0	41.0
56-57	38.367200722942734	40.0	37.0	41.0	34.0	41.0
58-59	38.223710886217546	40.0	37.0	41.0	34.0	41.0
60-61	38.05043339750877	40.0	36.5	41.0	34.0	41.0
62-63	37.83257550489391	39.5	36.0	41.0	33.0	41.0
64-65	37.59554021225614	39.0	36.0	41.0	33.0	41.0
66-67	37.33828023477785	39.0	35.0	41.0	33.0	41.0
68-69	36.98067757033988	38.0	35.0	40.0	32.5	41.0
70-71	36.674040037988746	37.0	35.0	40.0	32.0	41.0
72-73	36.39138835411809	37.0	35.0	39.5	32.0	41.0
74-75	36.05404121470412	36.0	35.0	39.0	32.0	41.0
76-77	35.713940482670736	36.0	35.0	39.0	32.0	40.0
78-79	35.373004098608426	35.5	35.0	37.5	31.0	39.5
80-81	35.06032799641922	35.0	34.0	37.0	31.0	39.0
82-83	34.76444042214737	35.0	34.0	37.0	31.0	39.0
84-85	34.505585568455054	35.0	34.0	36.0	31.0	37.5
86-87	34.28340883078728	35.0	34.0	36.0	31.0	37.0
88-89	34.23073821601888	35.0	34.0	36.0	31.0	37.0
90-91	34.195424409515276	35.0	34.0	35.0	31.0	36.0
92-93	34.117061804958325	35.0	34.0	35.0	31.0	36.0
94-95	34.15971839595102	35.0	34.0	35.0	32.0	36.0
96-97	34.20964022296956	35.0	34.0	35.0	32.0	36.0
98-99	34.31365894916158	35.0	35.0	35.0	32.0	36.0
100	34.09751578082254	35.0	34.0	35.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	21.0
20	89.0
21	534.0
22	2453.0
23	8464.0
24	22453.0
25	48250.0
26	88952.0
27	143866.0
28	209417.0
29	283266.0
30	367114.0
31	465018.0
32	586416.0
33	754953.0
34	1045728.0
35	1524490.0
36	2497291.0
37	5498999.0
38	9480723.0
39	6679573.0
40	471654.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.36478575053995	20.775778414953137	14.126384050007612	23.733051784499303
2	14.18543693869189	22.27751835785388	41.32262831014437	22.214416393309865
3	16.767547856464965	28.51533178266761	31.68380322604652	23.033317134820905
4	11.650718763980827	19.49417963569318	40.31282457633976	28.542277023986234
5	12.165947044633631	39.036417797536814	36.02732099077269	12.770314167056865
6	27.339512625131192	39.82132574696006	19.907228448661197	12.931933179247551
7	24.554271963900533	34.131032203539554	24.409661638412487	16.905034194147422
8	24.33758383293017	35.935236265687216	22.2231371133705	17.504042788012114
9	26.115238198998313	15.926372071718154	21.38117591984379	36.57721380943974
10-11	23.341714070318965	26.972882075182632	29.95126579104458	19.734138063453827
12-13	24.775106773699896	24.970102734464717	29.597967328890494	20.65682316294489
14-15	22.0268193754075	25.876015694142108	27.532273303191175	24.564891627259218
16-17	21.068133620563685	29.07044450966851	27.96179627343204	21.899625596335763
18-19	21.167798209963866	28.011667501553855	29.94331194391891	20.877222344563368
20-21	22.418364251402863	27.06926457204595	29.618164174626138	20.894207001925054
22-23	22.033234742771178	27.177867573098574	29.4325965566108	21.356301127519448
24-25	21.61780765392674	27.426571925383346	29.57368974947897	21.381930671210938
26-27	21.5994754546189	27.555470536442257	29.481745336951153	21.36330867198769
28-29	21.485082958277193	27.440129374460508	29.56056187815798	21.514225789104312
30-31	21.930052970187354	27.295132918063636	29.440191668561315	21.334622443187694
32-33	21.73398855557277	27.492899785993302	29.421842249846115	21.351269408587818
34-35	21.720065345353675	27.561015687070046	29.459388761850374	21.259530205725905
36-37	21.764373579854375	27.42181957386786	29.3393555081319	21.474451338145865
38-39	21.60010265215774	27.407171510734017	29.43380615661983	21.558919680488412
40-41	21.79576701323697	27.313186275146773	29.344195089686437	21.54685162192982
42-43	21.798015389053987	27.36043369282839	29.14197745931203	21.69957345880559
44-45	21.930794419615538	27.603575155384068	28.788045020864278	21.677585404136117
46-47	22.060624244258804	27.54625802886191	28.34069375109675	22.05242397578254
48-49	21.990983836441952	27.677115937286622	28.255834536530216	22.076065689741206
50-51	21.934693039077388	27.851349401201613	28.12040124178759	22.09355631793341
52-53	22.01543215165884	27.852036033895573	28.14685604252173	21.985675771923862
54-55	22.021873684645833	27.81847819658807	28.059512894392974	22.100135224373123
56-57	22.10983189155313	27.943688576204718	27.90783155701149	22.038647975230656
58-59	21.91979028568858	28.083222882637614	28.060827538900128	21.93615929277367
60-61	22.121981841082967	28.14663657360287	27.829168840404794	21.902212744909374
62-63	21.960976372176198	28.247360145607963	27.818634684721033	21.973028797494802
64-65	21.904173527269997	28.197022593304755	27.831415855842355	22.067388023582893
66-67	22.045436872178517	28.191989953141768	27.668899602398223	22.093673572281496
68-69	22.08566127574949	28.07701924990501	27.76959642493324	22.067723049412262
70-71	22.10144973356364	27.718747287721325	27.889056743870988	22.290746234844047
72-73	22.148837816717226	27.73394278342936	27.850104028324203	22.267115371529208
74-75	21.985921881175884	27.911182471942826	27.804754701878686	22.2981409450026
76-77	22.132154849273768	27.87483522398172	27.725186020397807	22.267823906346706
78-79	22.13084445634413	27.902520331926794	27.688697895736624	22.277937315992453
80-81	22.118056201423407	27.872233916301287	27.66759643732362	22.342113444951686
82-83	22.180390624398505	27.816782569170527	27.767576115349556	22.23525069108141
84-85	22.250647048532795	27.82341944892341	27.678207374559232	22.247726127984564
86-87	22.05817162455544	27.901221582168894	27.77673157343673	22.263875219838937
88-89	22.177408256624414	27.85302625712529	27.724419016893364	22.24514646935693
90-91	22.227129868503013	27.833549790165296	27.787418010786823	22.151902330544864
92-93	22.27174050646217	27.853469555804427	27.68685539540026	22.187934542333142
94-95	22.101353278716886	27.878819544715423	27.77133003507023	22.248497141497463
96-97	22.153676806565233	27.997046847141355	27.90348920552178	21.94578714077163
98-99	23.209133742248277	29.946268911613494	28.0891583649586	18.75543898117963
100	22.170533079166017	30.895838327264517	21.935375042646587	24.99825355092288
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7228.0
1	7157.0
2	10659.0
3	14528.0
4	19235.0
5	33817.5
6	45570.5
7	51447.0
8	52914.0
9	47525.5
10	38782.5
11	29919.5
12	31679.0
13	46339.0
14	83583.5
15	138825.5
16	186513.5
17	214806.5
18	219262.5
19	200216.5
20	169071.5
21	139215.0
22	121481.5
23	125603.0
24	151363.5
25	196339.5
26	257972.0
27	325402.0
28	389756.0
29	462244.5
30	537527.5
31	611088.0
32	693056.5
33	772176.0
34	849512.5
35	923697.0
36	999724.5
37	1067506.0
38	1109141.0
39	1140494.0
40	1174643.5
41	1194231.5
42	1202077.0
43	1222030.0
44	1241723.5
45	1265957.5
46	1284923.5
47	1279942.0
48	1272690.0
49	1243350.5
50	1189103.5
51	1131501.0
52	1065455.5
53	995464.0
54	923649.0
55	846522.5
56	768779.0
57	687985.0
58	608369.0
59	532455.5
60	447355.0
61	363378.5
62	286813.0
63	219787.0
64	165730.0
65	122271.5
66	89686.5
67	68026.5
68	51566.5
69	37349.5
70	26297.0
71	17986.5
72	12227.5
73	8404.5
74	5633.5
75	3710.5
76	2582.5
77	1705.0
78	1169.5
79	864.5
80	622.5
81	438.0
82	325.0
83	234.0
84	176.5
85	121.0
86	86.5
87	77.5
88	60.5
89	47.0
90	37.0
91	22.5
92	14.0
93	10.0
94	7.0
95	5.5
96	5.5
97	8.0
98	12.0
99	15.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.06597806752784965
6	0.0
7	0.0
8	1.9218199661587385E-4
9	8.416246058695165E-4
10-11	4.970224050410531E-6
12-13	4.191555615846214E-4
14-15	0.006103435133904132
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.013955753764892677
24-25	0.0
26-27	1.8237325970316927E-5
28-29	0.0026492780684085403
30-31	0.01389041921042651
32-33	0.0
34-35	4.509154033603619E-5
36-37	9.222396842251322E-5
38-39	5.5657191771863396E-5
40-41	0.0
42-43	0.004820805425670188
44-45	0.021490640654118372
46-47	0.0
48-49	0.002715755095852391
50-51	3.1875124729232836E-4
52-53	0.0
54-55	1.4459404787277575E-5
56-57	0.015169027003454525
58-59	1.8367377451112747E-6
60-61	0.0
62-63	0.0
64-65	0.0
66-67	3.0480113992578322E-5
68-69	0.01591304848617361
70-71	0.02363142738256295
72-73	0.0
74-75	0.013256099615275668
76-77	0.0
78-79	0.017882704733024554
80-81	8.984006650941796E-5
82-83	0.014430214259994693
84-85	0.0
86-87	0.015389858407616742
88-89	0.031175144408482493
90-91	2.5828451899415743E-5
92-93	1.721037659353927E-4
94-95	0.014242824449158323
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	763.0
20-21	3377.0
22-23	6773.0
24-25	8065.0
26-27	14154.0
28-29	30938.0
30-31	66894.0
32-33	84665.0
34-35	109765.0
36-37	160130.0
38-39	205154.0
40-41	213642.0
42-43	251585.0
44-45	317700.0
46-47	303412.0
48-49	264527.0
50-51	225913.0
52-53	197950.0
54-55	207824.0
56-57	225663.0
58-59	244340.0
60-61	252798.0
62-63	231263.0
64-65	243455.0
66-67	258579.0
68-69	269635.0
70-71	276724.0
72-73	233016.0
74-75	227367.0
76-77	236795.0
78-79	252370.0
80-81	263971.0
82-83	248773.0
84-85	239140.0
86-87	243058.0
88-89	259658.0
90-91	278298.0
92-93	282757.0
94-95	320324.0
96-97	912434.0
98-99	1794652.0
100-101	1.9711425E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.90163528290013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.93378790614982	67.80316391849126
2	9.046267123969786	14.275305385743078
3	2.6021692888645225	6.1594623652305645
4	1.050540120136238	3.3155733363617412
5	0.5300463859093325	2.091076331201874
6	0.27783175609209965	1.3152827933511906
7	0.16599398875596633	0.9168038011983956
8	0.11088696217360121	0.6999330117640179
9	0.06953887474383846	0.49380578397194563
>10	0.2076611251385598	2.447014803153806
>50	0.003794207131379371	0.2014167687718301
>100	0.0013760063986064802	0.17813489438593108
>500	5.335208575828464E-5	0.030896671123386253
>1k	5.2902450444546107E-5	0.07213013525099518
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.313482700273687E-6
2	0.0	1.3253930801094748E-5	0.0	1.6567413501368436E-5	6.626965400547374E-6
3	0.0	1.9880896201642123E-5	0.0	3.9761792403284245E-5	6.626965400547374E-6
4	3.313482700273687E-6	7.62101021062948E-5	0.0	6.626965400547374E-5	9.940448100821061E-6
5	6.626965400547374E-6	8.946403290738955E-5	0.0	6.958313670574743E-5	9.940448100821061E-6
6	4.638875780383162E-5	8.946403290738955E-5	0.0	6.958313670574743E-5	1.6567413501368436E-5
7	4.638875780383162E-5	9.277751560766324E-5	0.0	9.940448100821061E-5	1.6567413501368436E-5
8	4.638875780383162E-5	1.2591234261040012E-4	0.0	1.0934492910903167E-4	1.9880896201642123E-5
9	4.638875780383162E-5	2.0212244471669492E-4	0.0	1.5573368691286328E-4	3.9761792403284245E-5
10-11	5.301572320437899E-5	2.054359274169686E-4	0.0	2.054359274169686E-4	4.804549915396846E-5
12-13	5.798594725478952E-5	2.054359274169686E-4	0.0	2.3360053036929494E-4	6.295617130520006E-5
14-15	6.295617130520006E-5	2.733623227725792E-4	0.0	2.5679490927121076E-4	7.289661940602112E-5
16-17	7.289661940602112E-5	2.8330277087340025E-4	0.0	2.700488400723055E-4	8.283706750684218E-5
18-19	7.62101021062948E-5	3.164375978761371E-4	0.0	2.849595122235371E-4	9.609099830793692E-5
20-21	8.283706750684218E-5	3.3797523542791606E-4	0.0	3.247213046268213E-4	1.0768818775889482E-4
22-23	8.283706750684218E-5	3.7111006243065295E-4	0.0	3.6116961432983185E-4	1.1597189450957904E-4
24-25	8.283706750684218E-5	4.0258814808325296E-4	0.0	3.992746653829793E-4	1.2591234261040012E-4
26-27	8.449380885697903E-5	4.2412578563503193E-4	0.0	5.069628531418741E-4	1.3419604936108434E-4
28-29	8.78072915572527E-5	4.2743926833530563E-4	0.0	9.592532417292323E-4	1.3916627341149486E-4
30-31	8.946403290738955E-5	5.450679041950216E-4	0.0	0.002612681109165802	1.4744998016217909E-4
32-33	9.11207742575264E-5	5.483813868952952E-4	0.0	0.005803564949529363	1.7064435906409488E-4
34-35	1.027179637084843E-4	6.196212649511795E-4	0.0	0.009797968344709293	1.7395784176436858E-4
36-37	1.0437470505862115E-4	6.925178843572007E-4	0.0	0.01643487419335749	1.8389828986518962E-4
38-39	1.0603144640875798E-4	7.157122632591164E-4	0.0	0.035021855400542735	1.9715222066628437E-4
40-41	1.0934492910903167E-4	7.654145037632217E-4	0.0	0.05680303393079182	2.0212244471669492E-4
42-43	1.1597189450957904E-4	7.935791067155481E-4	0.0	0.06947047829393813	2.2200334091833704E-4
44-45	1.1762863585971589E-4	8.697892088218428E-4	0.0	0.08416577406965192	2.286303063188844E-4
46-47	1.1928537720985273E-4	9.145212252755377E-4	0.0	0.09887929400021724	2.3194378901915809E-4
48-49	1.2094211855998957E-4	9.194914493259481E-4	0.0	0.11340891564091735	2.402274957698423E-4
50-51	1.4579323881204224E-4	0.0010238661543845694	0.0	0.12811746534743224	2.43540978470116E-4
52-53	1.524202042125896E-4	0.0010304931197851167	0.0	0.16307139435261936	2.5016794387066336E-4
54-55	1.5407694556272643E-4	0.0010437470505862113	0.0	0.20174139420616344	2.551381679210739E-4
56-57	1.5904716961313698E-4	0.0010636279467878536	0.0	0.23804059718766168	2.700488400723055E-4
58-59	1.5904716961313698E-4	0.001078538618939085	0.0	0.25833733546818816	2.849595122235371E-4
60-61	1.6898761771395803E-4	0.001101732997841001	0.0	0.2732827991877726	2.8827299492381077E-4
62-63	2.0212244471669492E-4	0.0011199571526925062	0.0	0.2852080234260576	2.96556701674495E-4
64-65	2.0212244471669492E-4	0.0012475262366530432	0.0	0.2928290336366871	3.015269257249055E-4
66-67	2.0709266876710544E-4	0.0012524964607034538	0.0	0.2995603737422931	3.031836670750424E-4
68-69	2.087494101172423E-4	0.0012872880290563274	0.0	0.3060498296107791	3.164375978761371E-4
70-71	2.1040615146737912E-4	0.0013088256666081064	0.0	0.3113928204649704	3.296915286772319E-4
72-73	2.3194378901915809E-4	0.0013154526320086537	0.0	0.3155379873230128	3.4791568352873716E-4
74-75	2.3194378901915809E-4	0.0013585279071122117	0.0	0.3187305279047265	3.545426489292845E-4
76-77	2.3525727171943179E-4	0.0014330812678683695	0.0	0.3216795275079701	3.628263556799687E-4
78-79	2.3857075441970546E-4	0.001461245870820696	0.0	0.32362122837033047	3.661398383802424E-4
80-81	2.3857075441970546E-4	0.001461245870820696	0.0	0.3243203732200882	3.7939376918133717E-4
82-83	2.402274957698423E-4	0.001471186318921517	0.0	0.32470473721331994	4.0093140673311614E-4
84-85	2.4851120252052653E-4	0.0014910672151231593	0.0	0.32486212764158295	4.108718548339372E-4
86-87	2.5348142657093706E-4	0.0015391127142771275	0.0	0.3249068596580367	4.224690442848951E-4
88	2.683920987221686E-4	0.001597098661531917	0.0	0.3249134866234372	4.3075275103557933E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	46905	0.0	54.986755	1
GTATCAA	91630	0.0	40.256313	1
ATCAACG	114375	0.0	31.698488	3
TCAACGC	114340	0.0	31.674803	4
CAACGCA	116300	0.0	31.133694	5
TATCAAC	117720	0.0	31.017399	2
AACGCAG	119560	0.0	30.353191	6
ACGCAGA	135100	0.0	26.81822	7
CGCAGAG	136950	0.0	26.45904	8
GCAGAGT	158725	0.0	22.738152	9
GTACATG	127545	0.0	21.867573	1
TACATGG	126255	0.0	21.701357	2
ACATGGG	127785	0.0	20.897287	3
GAGTACT	86595	0.0	20.244167	12-13
CTTATAC	27940	0.0	20.083328	1
AGAGTAC	146250	0.0	19.753073	10-11
CAGAGTA	155455	0.0	19.604631	10-11
CATGGGG	81615	0.0	19.072216	4
AGTACTT	93050	0.0	17.9879	12-13
GTACTTT	97350	0.0	17.95161	14-15
>>END_MODULE