FastQCFastQC Report
Fri 27 May 2016
SRR2139911_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139911_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9910523
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177500.043.2730061
GTATCAA318600.037.298151
TCAACGC395100.029.7561174
ATCAACG396100.029.6916123
CAACGCA399950.029.3637285
TATCAAC405450.029.1221942
AACGCAG405350.029.066096
ACGCAGA459650.025.5866577
CGCAGAG464600.025.3140498
GCAGAGT537500.021.7866299
GTACATG433450.020.9327241
TACATGG432350.020.733232
ACATGGG433650.020.1270543
AGAGTAC484450.019.2624710-11
GAGTACT271700.019.243512-13
CAGAGTA524300.018.2035510-11
CATGGGG270800.017.940514
AGTACTT292050.017.85220512-13
GTACTTT310050.016.7828514-15
ATGGGGG143500.015.2423845
Produced by FastQC (version 0.11.5)