##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139911_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9910523
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4116395269957	33.0	31.0	34.0	31.0	34.0
2	32.55906464270352	34.0	31.0	34.0	31.0	34.0
3	32.667981296244406	34.0	31.0	34.0	31.0	34.0
4	36.18840186335272	37.0	35.0	37.0	35.0	37.0
5	36.042127141019705	37.0	35.0	37.0	35.0	37.0
6	36.08851914273344	37.0	35.0	37.0	35.0	37.0
7	36.059566886631515	37.0	35.0	37.0	35.0	37.0
8	36.05269207286033	37.0	35.0	37.0	35.0	37.0
9	37.8907780144398	39.0	38.0	39.0	35.0	39.0
10-11	37.76724876174547	39.0	37.5	39.0	35.0	39.0
12-13	37.786031423366865	39.0	37.5	39.0	35.0	39.0
14-15	39.092320102581866	40.0	38.5	41.0	36.0	41.0
16-17	39.04820083662588	40.0	38.0	41.0	36.0	41.0
18-19	39.135849641840295	40.0	38.5	41.0	36.0	41.0
20-21	39.142625472249456	40.0	39.0	41.0	36.0	41.0
22-23	39.10163038210569	40.0	39.0	41.0	36.0	41.0
24-25	39.08009373766458	40.0	39.0	41.0	36.0	41.0
26-27	38.986791724404995	40.0	38.5	41.0	35.5	41.0
28-29	38.904740966351326	40.0	38.0	41.0	35.0	41.0
30-31	38.749059721321856	40.0	38.0	41.0	35.0	41.0
32-33	38.64574012417893	40.0	38.0	41.0	35.0	41.0
34-35	38.60275557541468	40.0	38.0	41.0	35.0	41.0
36-37	38.50659358014404	40.0	38.0	41.0	34.5	41.0
38-39	38.47027103386183	40.0	38.0	41.0	34.0	41.0
40-41	38.473661786512295	40.0	38.0	41.0	34.0	41.0
42-43	38.44168115035287	40.0	38.0	41.0	34.0	41.0
44-45	38.3409554566229	40.0	38.0	41.0	34.0	41.0
46-47	38.28992247678693	40.0	38.0	41.0	34.0	41.0
48-49	38.275098422224985	40.0	38.0	41.0	34.0	41.0
50-51	38.20174875752279	40.0	38.0	41.0	33.5	41.0
52-53	38.07632465141974	40.0	37.0	41.0	33.0	41.0
54-55	37.908822355794946	40.0	37.0	41.0	33.0	41.0
56-57	37.78710007482562	39.5	37.0	41.0	33.0	41.0
58-59	37.570245552783206	39.0	36.0	41.0	33.0	41.0
60-61	37.41169251418439	39.0	36.0	40.5	32.0	41.0
62-63	37.20602907137296	39.0	35.5	40.0	32.0	41.0
64-65	36.90242867771681	38.5	35.0	40.0	31.5	41.0
66-67	36.834744489823024	38.0	35.0	40.0	32.0	41.0
68-69	36.60622577162614	37.5	35.0	40.0	31.5	41.0
70-71	36.29484776591963	37.0	35.0	39.5	31.0	41.0
72-73	35.98006463276057	36.5	35.0	39.0	31.0	41.0
74-75	35.582554565196496	36.0	34.0	39.0	31.0	40.0
76-77	33.52625098759941	34.5	32.0	36.5	28.0	39.0
78-79	34.72121698830895	35.0	34.0	37.0	30.0	39.0
80-81	34.72067669693332	35.0	34.0	37.0	31.0	39.0
82-83	34.42605999143758	35.0	34.0	36.5	30.0	39.0
84-85	34.158832434296045	35.0	34.0	36.0	30.0	37.0
86-87	33.94510797233497	35.0	34.0	36.0	30.0	37.0
88-89	33.7052684902499	35.0	34.0	35.0	30.0	37.0
90-91	33.53791663359714	35.0	33.5	35.0	30.0	36.0
92-93	33.47020334374314	35.0	33.0	35.0	30.0	36.0
94-95	33.43953463193479	35.0	33.0	35.0	30.0	36.0
96-97	33.46899467099939	35.0	33.0	35.0	30.0	35.5
98-99	33.49292713610339	35.0	33.0	35.0	30.0	35.0
100-101	32.20602199365142	33.5	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	3.0
18	7.0
19	17.0
20	65.0
21	201.0
22	792.0
23	2636.0
24	6909.0
25	15156.0
26	28356.0
27	47091.0
28	70237.0
29	97941.0
30	131251.0
31	173571.0
32	230199.0
33	317054.0
34	468909.0
35	729651.0
36	1316468.0
37	2423244.0
38	2777221.0
39	1058052.0
40	15490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.656671398673915	20.970053749938323	14.171815150421427	23.201459700966335
2	14.277764256240793	22.518091614669295	41.321161124652825	21.882983004437083
3	16.999372081320868	28.57128874885362	31.70208341258414	22.727255757241373
4	11.642796247988123	19.426290620585814	40.63822867874884	28.292684452677218
5	12.152709738364731	39.09518719210729	36.15013308088763	12.601969988640347
6	27.337558270133677	39.90527038784936	19.938473479149383	12.818697862867582
7	24.53561734330267	34.08810009320396	24.60531094070414	16.770971622789233
8	24.24428973052561	35.94672224689869	22.381120264834856	17.42786775774085
9	26.090864537661073	15.912902977454946	21.555528266907732	36.44070421797625
10-11	23.24533272401439	27.057903176307075	30.116986311059307	19.579777788619232
12-13	24.645246999466323	25.10543124671875	29.816118017207426	20.433203736607496
14-15	21.9412380035318	26.094859190551134	27.71000636596199	24.253896439955074
16-17	20.945304118714024	29.331226605554207	28.12428106350535	21.59918821222642
18-19	21.01377919485767	28.387332008075056	30.001480245084995	20.597408551982284
20-21	22.279034034598922	27.42755085427185	29.636487761256653	20.65692734987258
22-23	21.840662251949713	27.525232697110546	29.437150838270735	21.196954212669
24-25	21.377630748334838	27.785187803259582	29.61090097530675	21.226280473098832
26-27	21.340404394087045	27.90120141444107	29.5128686040883	21.245525587383586
28-29	21.238508925290287	27.94152994163393	29.529898786430543	21.290062346645243
30-31	21.51415426261553	27.84075680232172	29.42403453268402	21.221054402378726
32-33	21.289616866221746	28.048399724193455	29.36651484043629	21.2954685691485
34-35	21.393687311069527	28.164966624564826	29.388714753501493	21.052631310864157
36-37	21.434927079662238	28.088873669158726	29.184914346788204	21.291284904390835
38-39	21.36945475270384	28.12401604150055	29.234596532424572	21.271932673371037
40-41	21.524612681627115	28.04606843863311	29.14266575829288	21.2866531214469
42-43	21.542692235852257	28.129355303174986	28.911877006474306	21.41607545449845
44-45	21.566786999400485	28.314048983155804	28.610834037875843	21.50832997956787
46-47	21.729467335731147	28.26861994264196	28.246799356789005	21.75511336483789
48-49	21.696239323075222	28.384307814702897	28.156223410613624	21.76322945160826
50-51	21.66745068368477	28.519715560818888	28.038778687895434	21.77405506760091
52-53	21.811589007252756	28.45547103522723	27.946606359074046	21.78633359844597
54-55	21.77354581733767	28.361471641200815	27.975539299899886	21.889443241561633
56-57	21.86551932030866	28.424959456848747	27.869205353109294	21.840315869733296
58-59	21.79158544862208	28.451673562643858	27.95298917841924	21.80375181031482
60-61	21.88068461101159	28.4764485856896	27.847614133852144	21.79525266944667
62-63	21.805791175018562	28.493369786298018	27.83427100144068	21.866568037242743
64-65	21.782547992057683	28.396970000505817	27.83862001517919	21.981861992257315
66-67	21.835769904243456	28.46538028548436	27.746036364291154	21.95281344598103
68-69	21.907191193788204	28.390065292269316	27.738280606361425	21.96446290758105
70-71	21.907883381688936	28.150302671069692	27.8184340709221	22.123379876319273
72-73	21.898816382546023	28.157743025912417	27.808686299583236	22.13475429195832
74-75	21.843821995111046	28.170256807898898	27.80568240933948	22.180238787650573
76-77	21.921109757782588	28.136758503014047	27.758162656726277	22.183969082477084
78-79	21.939387322377808	28.159347175014172	27.686766714837507	22.214498787770513
80-81	21.95829329597767	28.087790255873312	27.716628586313018	22.237287861836
82-83	21.969696428815986	28.02837262606524	27.81305439624928	22.188876548869494
84-85	22.080748438879898	28.017327864852327	27.710629177095925	22.19129451917185
86-87	21.96100816744187	28.007164670080364	27.823704524738318	22.208122637739443
88-89	22.084570477295816	27.923606954165887	27.763744402401596	22.2280781661367
90-91	22.06459313370919	27.909404769844	27.88447996662101	22.141522129825802
92-93	22.02111062365257	27.805402874287577	27.957565478963236	22.215921023096612
94-95	21.912199795066243	27.839290980970265	27.98467218271419	22.2638370412493
96-97	21.910068320314874	27.8419724297093	28.04375955939574	22.204199690580086
98-99	22.152349801371518	28.471756005957317	28.11445095019937	21.2614432424718
100-101	22.954638726452185	31.027462045168207	25.22054107398104	20.79735815439857
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1283.0
1	1411.5
2	3155.5
3	4983.5
4	7055.0
5	12457.0
6	16596.0
7	18489.5
8	19008.5
9	17527.0
10	15240.5
11	13210.0
12	14716.0
13	21282.5
14	35960.5
15	55992.0
16	72168.0
17	80915.5
18	81766.0
19	75610.0
20	66121.5
21	57207.5
22	52427.0
23	54957.0
24	64973.5
25	81058.0
26	102085.5
27	124749.5
28	148028.0
29	171738.5
30	193693.0
31	216322.0
32	240154.0
33	263726.5
34	287730.0
35	310304.0
36	333277.0
37	352907.0
38	365382.0
39	375586.5
40	387132.0
41	396419.0
42	401387.0
43	407311.0
44	412656.5
45	415423.5
46	416428.5
47	417061.0
48	414432.0
49	406658.5
50	394330.0
51	375354.5
52	351527.5
53	325897.0
54	299386.5
55	271406.0
56	244640.5
57	218454.5
58	191428.0
59	164654.5
60	137523.5
61	110696.5
62	86448.5
63	66621.5
64	50052.0
65	37069.5
66	27528.0
67	20410.0
68	15293.5
69	11106.5
70	8009.5
71	5857.0
72	4217.5
73	3029.0
74	2142.5
75	1489.0
76	1004.5
77	656.0
78	442.5
79	311.0
80	231.5
81	173.0
82	133.5
83	102.5
84	76.0
85	57.5
86	39.5
87	28.0
88	20.5
89	17.0
90	16.0
91	13.5
92	11.0
93	9.5
94	6.5
95	4.0
96	3.0
97	2.5
98	2.5
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004106745930562898
3	4.0361139366711526E-5
4	0.0
5	9.081256357510093E-5
6	0.0
7	0.0
8	4.5406281787550466E-4
9	1.2108341810013456E-4
10-11	4.5406281787550466E-5
12-13	3.632502543004037E-4
14-15	2.0180569683355763E-5
16-17	5.14604526925572E-4
18-19	2.0180569683355763E-5
20-21	7.063491250629974E-5
22-23	0.0
24-25	0.0
26-27	1.0604358017467802E-4
28-29	5.052770631195139E-6
30-31	7.085325026633231E-5
32-33	4.4154024870287403E-4
34-35	0.0
36-37	3.839042757722618E-4
38-39	0.0011340846884958036
40-41	0.0
42-43	0.0
44-45	3.498186428849353E-4
46-47	0.0
48-49	0.0
50-51	0.0
52-53	5.80898690106648E-4
54-55	0.0033585859572053665
56-57	0.003710558007113911
58-59	0.004411532792753807
60-61	9.648042288747643E-4
62-63	1.1605280727678953E-5
64-65	5.626664811430242E-4
66-67	0.0
68-69	0.0
70-71	6.058207010629852E-6
72-73	1.5319426276455962E-4
74-75	2.4781165273583134E-5
76-77	0.0
78-79	0.0
80-81	4.6147432843948353E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0011832985548436308
92-93	1.3903092249311085E-4
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	213.0
20-21	1336.0
22-23	2613.0
24-25	3606.0
26-27	5321.0
28-29	11690.0
30-31	26279.0
32-33	33170.0
34-35	43725.0
36-37	63110.0
38-39	79125.0
40-41	82603.0
42-43	96457.0
44-45	114029.0
46-47	107493.0
48-49	97590.0
50-51	85715.0
52-53	74573.0
54-55	80509.0
56-57	84785.0
58-59	86781.0
60-61	92109.0
62-63	85489.0
64-65	87436.0
66-67	93687.0
68-69	93721.0
70-71	95282.0
72-73	89451.0
74-75	88989.0
76-77	85707.0
78-79	92566.0
80-81	98029.0
82-83	93943.0
84-85	92629.0
86-87	95546.0
88-89	104473.0
90-91	119642.0
92-93	117151.0
94-95	131324.0
96-97	196405.0
98-99	831065.0
100-101	5945156.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.64939972782784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.27311291640852	75.56915420208577
2	7.505884050356936	12.707371585787836
3	1.81616386000651	4.612115416707774
4	0.6782602437950541	2.29657289986006
5	0.3001674003879421	1.2704495130350928
6	0.14355844598807613	0.7291281767250264
7	0.08389754526805451	0.49713137919053546
8	0.050542440652569666	0.34227098096155084
9	0.03540511687672207	0.26973197018172995
>10	0.10949304087047547	1.4382340556871251
>50	0.002791074802505499	0.1625808588349112
>100	7.04040375974459E-4	0.09223684649813171
>500	1.9824210648899303E-5	0.013022114444496947
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.0180569683355763E-5	0.0	1.0090284841677881E-5	0.0
3	0.0	3.0270854525033643E-5	0.0	1.0090284841677881E-5	0.0
4	0.0	7.063199389174517E-5	0.0	1.0090284841677881E-5	1.0090284841677881E-5
5	0.0	9.081256357510093E-5	0.0	1.0090284841677881E-5	1.0090284841677881E-5
6	0.0	9.081256357510093E-5	0.0	2.0180569683355763E-5	2.0180569683355763E-5
7	0.0	1.0090284841677881E-4	0.0	3.0270854525033643E-5	2.0180569683355763E-5
8	0.0	1.0090284841677881E-4	0.0	4.0361139366711526E-5	2.0180569683355763E-5
9	0.0	1.4126398778349034E-4	0.0	7.063199389174517E-5	3.0270854525033643E-5
10-11	1.0090284841677881E-5	1.4126398778349034E-4	0.0	1.0594799083761774E-4	3.0270854525033643E-5
12-13	1.0090284841677881E-5	1.4126398778349034E-4	0.0	1.261285605209735E-4	3.5315996945872586E-5
14-15	1.0090284841677881E-5	2.472119786211081E-4	0.0	1.5639941504600716E-4	5.5496566629228346E-5
16-17	2.0180569683355763E-5	2.724376907253028E-4	0.0	1.8162512715020186E-4	6.0541709050067285E-5
18-19	2.0180569683355763E-5	2.875731179878196E-4	0.0	2.774828331461417E-4	9.081256357510093E-5
20-21	2.0180569683355763E-5	3.027085452503364E-4	0.0	4.1370167850879314E-4	1.1099313325845669E-4
22-23	2.0180569683355763E-5	3.279342573545311E-4	0.0	9.686673448010767E-4	1.2612856052097353E-4
24-25	2.0180569683355763E-5	3.4306968461704794E-4	0.0	0.003052311164607559	1.6648969988768505E-4
26-27	3.0270854525033643E-5	3.632502543004037E-4	0.0	0.006876529119603476	1.7153484230852397E-4
28-29	3.0270854525033643E-5	3.834308239837595E-4	0.0	0.02674934511528806	1.7153484230852397E-4
30-31	3.0270854525033643E-5	4.3388224819214887E-4	0.0	0.03882237092835565	1.7153484230852397E-4
32-33	3.5315996945872586E-5	4.3388224819214887E-4	0.0	0.04339326996163573	1.7153484230852397E-4
34-35	4.0361139366711526E-5	4.944239572422162E-4	0.0	0.04922545460012555	1.7153484230852397E-4
36-37	4.0361139366711526E-5	5.448753814506056E-4	0.0	0.056899116222221574	1.7153484230852397E-4
38-39	4.0361139366711526E-5	5.600108087131224E-4	0.0	0.07200427263021336	1.9171541199187973E-4
40-41	4.0361139366711526E-5	5.852365208173172E-4	0.0	0.10690152275515633	1.9171541199187973E-4
42-43	4.0361139366711526E-5	5.95326805658995E-4	0.0	0.11944879195578276	1.9676055441271868E-4
44-45	4.0361139366711526E-5	6.407330874465454E-4	0.0	0.13696552644093557	2.0685083925439657E-4
46-47	4.0361139366711526E-5	6.810942268132569E-4	0.0	0.15772124236026697	2.1694112409607446E-4
48-49	4.0361139366711526E-5	6.861393692340959E-4	0.0	0.17466283060944413	2.3207655135859128E-4
50-51	4.0361139366711526E-5	7.315456510216464E-4	0.0	0.1933147221392857	2.3207655135859128E-4
52-53	4.0361139366711526E-5	7.416359358633243E-4	0.0	0.21713788465048717	2.371216937794302E-4
54-55	4.0361139366711526E-5	7.517262207050021E-4	0.0	0.2627056109955045	2.4216683620026914E-4
56-57	4.0361139366711526E-5	7.567713631258411E-4	0.0	0.3139945288457532	2.472119786211081E-4
58-59	4.0361139366711526E-5	7.668616479675189E-4	0.0	0.3674579030793834	2.5730226346278593E-4
60-61	4.0361139366711526E-5	7.870422176508747E-4	0.0	0.4029706605796687	2.623474058836249E-4
62-63	4.0361139366711526E-5	8.021776449133916E-4	0.0	0.4188527689204697	2.774828331461417E-4
64-65	4.0361139366711526E-5	9.484867751177208E-4	0.0	0.43372584877710285	2.8757311798781966E-4
66-67	4.0361139366711526E-5	9.585770599593987E-4	0.0	0.4397396585427429	2.926182604086586E-4
68-69	4.0361139366711526E-5	9.636222023802377E-4	0.0	0.4452439089238782	2.926182604086586E-4
70-71	4.0361139366711526E-5	9.888479144844324E-4	0.0	0.4514090729621434	2.926182604086586E-4
72-73	4.0361139366711526E-5	0.001014073626588627	0.0	0.456151506837732	3.228891149336922E-4
74-75	4.0361139366711526E-5	0.0010645250507970165	0.0	0.4599656345078862	3.38024542196209E-4
76-77	4.0361139366711526E-5	0.0011200216174262448	0.0	0.46316425480269807	3.5315996945872583E-4
78-79	4.0361139366711526E-5	0.0011402021871096005	0.0	0.4660753019795222	3.5820511187956475E-4
80-81	4.0361139366711526E-5	0.0011402021871096005	0.0	0.46748289671493626	3.7838568156292054E-4
82-83	4.0361139366711526E-5	0.0011654278992137951	0.0	0.46814381037206615	4.0361139366711525E-4
84-85	4.0361139366711526E-5	0.001200743896159668	0.0	0.46864832461415	4.1370167850879314E-4
86-87	4.0361139366711526E-5	0.0012411050355263793	0.0	0.46889553659277117	4.2883710577130995E-4
88-89	4.0361139366711526E-5	0.0013117370294181245	0.0	0.4690065297260296	4.4397253303382676E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17750	0.0	43.273006	1
GTATCAA	31860	0.0	37.29815	1
TCAACGC	39510	0.0	29.756117	4
ATCAACG	39610	0.0	29.691612	3
CAACGCA	39995	0.0	29.363728	5
TATCAAC	40545	0.0	29.122194	2
AACGCAG	40535	0.0	29.06609	6
ACGCAGA	45965	0.0	25.586657	7
CGCAGAG	46460	0.0	25.314049	8
GCAGAGT	53750	0.0	21.786629	9
GTACATG	43345	0.0	20.932724	1
TACATGG	43235	0.0	20.73323	2
ACATGGG	43365	0.0	20.127054	3
AGAGTAC	48445	0.0	19.26247	10-11
GAGTACT	27170	0.0	19.2435	12-13
CAGAGTA	52430	0.0	18.20355	10-11
CATGGGG	27080	0.0	17.94051	4
AGTACTT	29205	0.0	17.852205	12-13
GTACTTT	31005	0.0	16.78285	14-15
ATGGGGG	14350	0.0	15.242384	5
>>END_MODULE