FastQCFastQC Report
Fri 27 May 2016
SRR2139910_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139910_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15367658
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA234650.051.763981
GTATCAA449700.039.1421
TCAACGC557500.031.0185764
ATCAACG559550.030.8983863
CAACGCA565950.030.5250745
TATCAAC577350.030.1875062
AACGCAG584500.029.574026
ACGCAGA667550.025.8051787
CGCAGAG674450.025.544778
GCAGAGT777200.021.896359
GTACATG557350.020.6321741
TACATGG555550.020.272712
GAGTACT446850.019.79686412-13
ACATGGG566550.019.498393
AGAGTAC713600.019.26262310-11
CAGAGTA760500.018.87031710-11
AGTACTT484650.017.63946212-13
GTACTTT503150.017.56547714-15
CATGGGG355550.017.259034
GTGGTAT97300.016.0824681
Produced by FastQC (version 0.11.5)