##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139910_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15367658
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.806363858435684	33.0	31.0	34.0	30.0	34.0
2	31.844641128791388	33.0	31.0	34.0	29.0	34.0
3	31.912520567545165	34.0	31.0	34.0	29.0	34.0
4	35.402727728584274	37.0	35.0	37.0	33.0	37.0
5	35.231720409186615	37.0	35.0	37.0	32.0	37.0
6	35.32507588339095	37.0	35.0	37.0	32.0	37.0
7	35.23857961961413	37.0	35.0	37.0	32.0	37.0
8	35.23253992247875	37.0	35.0	37.0	32.0	37.0
9	36.80096075797626	39.0	37.0	39.0	32.0	39.0
10-11	36.76829660706921	39.0	37.0	39.0	32.0	39.0
12-13	36.73371528049362	39.0	37.0	39.0	32.0	39.0
14-15	37.984080593152186	40.0	38.0	41.0	32.0	41.0
16-17	38.011592527631734	40.0	38.0	41.0	32.0	41.0
18-19	38.05305190289893	40.0	38.0	41.0	32.0	41.0
20-21	38.0943231509622	40.0	38.0	41.0	32.0	41.0
22-23	38.050259972639026	40.0	38.0	41.0	32.0	41.0
24-25	38.03027787931009	40.0	38.0	41.0	32.0	41.0
26-27	37.9682303282774	40.0	37.0	41.0	32.0	41.0
28-29	37.87958573351245	40.0	37.0	41.0	32.0	41.0
30-31	37.83496510341203	40.0	37.0	41.0	32.0	41.0
32-33	37.734461718684486	40.0	37.0	41.0	32.0	41.0
34-35	37.63834963835518	40.0	37.0	41.0	31.0	41.0
36-37	37.596678568224576	40.0	37.0	41.0	31.0	41.0
38-39	37.57594960009683	40.0	37.0	41.0	31.0	41.0
40-41	37.54462814996512	40.0	36.5	41.0	31.0	41.0
42-43	37.507661352182524	40.0	36.0	41.0	31.0	41.0
44-45	37.46484822671768	40.0	36.0	41.0	31.0	41.0
46-47	37.402268919428984	40.0	36.0	41.0	31.0	41.0
48-49	37.34897256817732	40.0	36.0	41.0	31.0	41.0
50-51	37.26446750871054	40.0	36.0	41.0	31.0	41.0
52-53	37.15982796123097	39.5	36.0	41.0	30.5	41.0
54-55	37.21146486265656	39.0	36.0	41.0	30.5	41.0
56-57	37.289772782265686	39.0	36.0	41.0	31.0	41.0
58-59	37.19571348818779	39.0	35.0	41.0	31.0	41.0
60-61	37.08295563671026	39.0	35.0	41.0	31.0	41.0
62-63	36.90513232249933	39.0	35.0	41.0	31.0	41.0
64-65	36.69789105601619	38.5	35.0	41.0	31.0	41.0
66-67	36.46416128175461	38.0	35.0	40.0	30.0	41.0
68-69	36.232771194806446	37.0	35.0	40.0	30.0	41.0
70-71	35.96521090366104	37.0	34.5	39.5	30.0	41.0
72-73	35.68744565132691	36.5	34.0	39.0	30.0	41.0
74-75	35.401012151230006	36.0	34.0	39.0	30.0	41.0
76-77	35.09993028899028	35.5	34.0	38.5	30.0	40.0
78-79	34.793240120195804	35.0	34.0	37.0	29.5	39.0
80-81	34.52615488280979	35.0	34.0	37.0	29.5	39.0
82-83	34.24969862333532	35.0	34.0	36.5	29.0	39.0
84-85	34.018131854795726	35.0	34.0	36.0	29.0	37.0
86-87	33.823332530357405	35.0	34.0	36.0	29.0	37.0
88-89	33.66955422860992	35.0	33.0	35.0	29.0	37.0
90-91	33.557087653218176	35.0	33.0	35.0	29.0	36.0
92-93	33.47997746969894	35.0	33.0	35.0	29.0	36.0
94-95	33.518410149021676	35.0	33.5	35.0	29.5	36.0
96-97	33.669595943958896	35.0	34.0	35.0	30.0	36.0
98-99	33.748717625509066	35.0	34.0	35.0	30.0	36.0
100	33.10795126254897	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	14.0
16	37.0
17	189.0
18	1343.0
19	6827.0
20	21129.0
21	46561.0
22	77804.0
23	106229.0
24	133593.0
25	160059.0
26	184913.0
27	210008.0
28	238088.0
29	273432.0
30	316752.0
31	373826.0
32	452519.0
33	562287.0
34	733380.0
35	989755.0
36	1475095.0
37	2638164.0
38	3886076.0
39	2356673.0
40	122900.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.3071242215307	21.231101056517527	14.441823210797638	24.01995151115414
2	14.218822133252498	22.68392999172357	40.67573243295199	22.421515442071943
3	16.773989161109213	28.397565870119173	31.598491697463803	23.229953271307814
4	11.866437972038078	19.71201275445125	40.1034251502879	28.31812412322277
5	12.129441859071791	39.110846712799614	36.14750169644729	12.6122097316813
6	27.41184062105038	40.252630995331685	19.645906116471153	12.689622267146778
7	24.35534098810187	34.09344773575716	24.671660514165332	16.879550761975644
8	23.990777770786178	36.397163105799315	22.169816323913132	17.44224279950137
9	25.799477590199505	16.083541579511063	21.55243987214944	36.56454095813999
10-11	23.152378182177852	27.238560687992024	30.044801992761407	19.56425913706872
12-13	24.531108936556436	25.193835853281847	29.784234302178564	20.49082090798315
14-15	21.808550788936092	26.158650849726094	27.66393877323402	24.368859588103795
16-17	20.900601770289263	29.21491030058061	28.168000615318224	21.716487313811903
18-19	20.95828931498613	28.281037855832825	30.116621642418213	20.644051186762837
20-21	22.124510767988433	27.34806293428499	29.85789641631446	20.669529881412114
22-23	21.703594486257188	27.385815345440463	29.710640275575663	21.199949892726693
24-25	21.36806032476663	27.57310955868668	29.801387210692155	21.257442905854536
26-27	21.330005812044092	27.692049410509238	29.691015584713163	21.286929192733503
28-29	21.319776176625417	27.62334100462951	29.776068978386146	21.280813840358928
30-31	21.60929089491354	27.51915477356136	29.63584153755577	21.235712793969334
32-33	21.427989473437595	27.683723235751774	29.5476286935959	21.34065859721473
34-35	21.53502222422204	27.760949480054993	29.520849254913806	21.183179040809165
36-37	21.54941941086173	27.694457901872692	29.388985179346715	21.36713750791886
38-39	21.411122632304565	27.748673069813755	29.456443943337106	21.383760354544577
40-41	21.657803927871075	27.652724257441935	29.306275497451416	21.383196317235573
42-43	21.600891249275097	27.747442067101442	29.105963027398797	21.54570365622467
44-45	21.690472673344914	27.92730008953891	28.81080656394997	21.571420673166212
46-47	21.790806587250167	27.958619491963688	28.433014992005273	21.817558928780873
48-49	21.78914869858872	28.02785241147217	28.3724977572603	21.81050113267881
50-51	21.773516430978827	28.189984550889214	28.159627499755874	21.87687151837609
52-53	21.89804024337329	28.130280884301705	28.133981435815407	21.837697436509593
54-55	21.88700774598554	28.056537671963888	28.201393475149672	21.8550611069009
56-57	21.87808033469565	28.138699502005636	28.1146488840831	21.868571279215615
58-59	21.850342751359367	28.204911903343987	28.172755535764534	21.771989809532112
60-61	21.964192102907138	28.16975599465037	28.081328338789973	21.784723563652516
62-63	21.86393649940654	28.25691285471023	28.069452571264037	21.809698074619195
64-65	21.85098013815218	28.19518799279368	28.079763934369467	21.874067934684668
66-67	21.922733960203946	28.211427991887174	27.977726041372858	21.888112006536023
68-69	21.894652689361806	28.15406741994258	28.0374497936416	21.913830097054014
70-71	21.91822487563074	27.939661007774465	28.09221214794405	22.049901968650744
72-73	21.940230866128584	28.01300792469663	28.06468189549242	21.982079313682366
74-75	21.81108001137084	28.105430983046826	28.04215724250821	22.041331763074126
76-77	21.897741444337406	28.074750756006388	28.033708748496984	21.993799051159225
78-79	21.913045166788685	28.099721816253247	27.964193238285862	22.023039778672203
80-81	21.90674599983919	28.085032564123182	27.962330143925385	22.045891292112245
82-83	21.90955116075221	28.07254848079774	28.047945899838687	21.96995445861137
84-85	21.969421685175035	28.07462390053847	27.970435162668554	21.985519251617937
86-87	21.84692904877353	28.10786994489012	28.04788402789088	21.997316978445475
88-89	21.888790272923877	28.072520204621604	28.00904573114976	22.029643791304757
90-91	21.954038222998314	28.101058036679728	28.03222379762751	21.912679942694453
92-93	21.953193053121257	28.102866994842973	27.991847844200763	21.95209210783501
94-95	21.778547828189218	28.159869651931864	28.05071570383038	22.010866816048537
96-97	21.882169705387824	28.312647193657213	28.089327007801916	21.715856093153043
98-99	22.904401371472346	30.262185458649522	28.28272674801734	18.550686421860792
100	21.88080596439204	31.23749853934824	22.10840482271832	24.7732906735414
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2890.0
1	3042.0
2	5386.0
3	7747.0
4	9810.5
5	16492.0
6	22259.0
7	25721.0
8	27558.5
9	25752.5
10	21557.5
11	16995.5
12	17569.5
13	24841.5
14	43676.5
15	71010.5
16	93851.5
17	107234.0
18	109913.5
19	103043.0
20	91502.5
21	80673.0
22	75751.0
23	80593.0
24	95378.5
25	118843.5
26	149419.5
27	182881.0
28	216960.0
29	254948.0
30	292420.5
31	329334.0
32	371042.0
33	411901.5
34	452092.0
35	488549.0
36	523245.0
37	556216.5
38	578438.0
39	594738.5
40	611143.0
41	623710.5
42	630851.5
43	638631.0
44	646878.5
45	652723.5
46	650154.0
47	642380.0
48	633529.5
49	618078.5
50	597183.0
51	569175.0
52	535742.5
53	499713.0
54	460938.5
55	421151.0
56	383327.0
57	344577.0
58	305943.5
59	267635.0
60	224828.0
61	182563.0
62	143598.5
63	110249.5
64	84387.0
65	64076.0
66	47876.0
67	36646.0
68	28467.5
69	21439.5
70	16021.0
71	11846.5
72	8849.5
73	6692.0
74	4882.0
75	3542.5
76	2506.0
77	1725.0
78	1284.5
79	986.0
80	721.0
81	533.5
82	405.0
83	276.5
84	196.5
85	146.0
86	118.5
87	96.0
88	63.5
89	38.0
90	27.5
91	21.5
92	17.5
93	11.0
94	5.5
95	4.0
96	3.0
97	3.5
98	3.0
99	1.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007366119157518993
3	0.00982583032495908
4	0.21532233473701717
5	0.07833334135884595
6	0.0042947337844192
7	0.010086117220984486
8	4.555020680444607E-5
9	1.887079996184194E-4
10-11	2.4727255122413577E-4
12-13	1.0411475841016243E-4
14-15	0.0
16-17	0.0
18-19	8.459324120825698E-5
20-21	0.003985331894793489
22-23	0.011101120681925612
24-25	1.957714540552784E-5
26-27	7.187054024921764E-5
28-29	9.848461300618555E-4
30-31	0.0022989397624326156
32-33	0.0038859742619519787
34-35	0.014966646937954842
36-37	0.008782378923405805
38-39	0.004265153611494606
40-41	0.004021014122642815
42-43	0.002492587096843113
44-45	0.0036218212057494664
46-47	0.0013912408376668005
48-49	5.5932998420522045E-5
50-51	0.02481571264900185
52-53	1.0664412446862343E-5
54-55	0.0
56-57	0.01259412156651813
58-59	3.712015977681322E-4
60-61	2.899499671145354E-4
62-63	4.7742909539510753E-4
64-65	5.559247833021985E-4
66-67	1.2418728827948974E-4
68-69	0.0011050621720889396
70-71	0.0
72-73	1.4318386743495775E-4
74-75	1.1714465525753217E-5
76-77	1.1821988283463852E-5
78-79	0.0
80-81	3.61823459981863E-5
82-83	0.0
84-85	4.516020171009368E-5
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	8.72149358019579E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	7703.0
20-21	15116.0
22-23	16642.0
24-25	17853.0
26-27	21876.0
28-29	30672.0
30-31	48587.0
32-33	56111.0
34-35	68874.0
36-37	87296.0
38-39	110091.0
40-41	112580.0
42-43	130068.0
44-45	157538.0
46-47	150194.0
48-49	130484.0
50-51	114316.0
52-53	104682.0
54-55	107238.0
56-57	112253.0
58-59	115195.0
60-61	115204.0
62-63	108529.0
64-65	112797.0
66-67	118810.0
68-69	122608.0
70-71	124449.0
72-73	116703.0
74-75	115213.0
76-77	121066.0
78-79	126763.0
80-81	132209.0
82-83	127822.0
84-85	125588.0
86-87	129658.0
88-89	140266.0
90-91	149590.0
92-93	157539.0
94-95	180619.0
96-97	488977.0
98-99	945035.0
100-101	9892844.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.82188257101632
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.88654361370614	74.50637420931596
2	7.632924444126879	12.796121928580865
3	1.952228584535648	4.909184254941853
4	0.7059769768416941	2.367052770026624
5	0.32600736314306417	1.3663275455332298
6	0.16662338464642834	0.838001146885092
7	0.09803793167844427	0.5752406797659113
8	0.061888078916198984	0.41500602267675374
9	0.0397347066362177	0.29975741222893404
>10	0.12587702535179676	1.5906717546359552
>50	0.0031086291111671674	0.16828743155252113
>100	0.0010083726497178867	0.1429108368352733
>500	4.0888656582882626E-5	0.025064007020948454
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.507172400635152E-6	0.0
2	0.0	1.3014344801270304E-5	0.0	6.507172400635152E-6	0.0
3	0.0	1.9521517201905457E-5	0.0	6.507172400635152E-6	0.0
4	0.0	7.808606880762183E-5	0.0	6.507172400635152E-6	0.0
5	0.0	7.808606880762183E-5	0.0	6.507172400635152E-6	0.0
6	0.0	8.459324120825698E-5	0.0	1.9521517201905457E-5	1.3014344801270304E-5
7	0.0	8.459324120825698E-5	0.0	3.253586200317576E-5	1.3014344801270304E-5
8	6.507172400635152E-6	8.459324120825698E-5	0.0	3.253586200317576E-5	1.9521517201905457E-5
9	6.507172400635152E-6	1.3014344801270304E-4	0.0	8.459324120825698E-5	3.253586200317576E-5
10-11	2.6028689602540607E-5	1.3014344801270304E-4	0.0	1.2038268941175032E-4	3.253586200317576E-5
12-13	2.6028689602540607E-5	1.3014344801270304E-4	0.0	1.4315779281397336E-4	3.253586200317576E-5
14-15	4.229662060412849E-5	1.724400686168315E-4	0.0	1.496649652146085E-4	4.555020680444606E-5
16-17	7.483248260730425E-5	1.8220082721778425E-4	0.0	1.496649652146085E-4	5.856455160571637E-5
18-19	9.43539998092097E-5	2.0497593062000728E-4	0.0	1.5942572381556124E-4	6.181813780603394E-5
20-21	1.0411475841016243E-4	2.277510340222303E-4	0.0	1.9196158581873699E-4	6.507172400635152E-5
22-23	1.1062193081079759E-4	2.6679406842604125E-4	0.0	2.0822951682032486E-4	6.83253102066691E-5
24-25	1.1387551701111517E-4	3.0258351662953455E-4	0.0	2.3751179262318305E-4	7.808606880762183E-5
26-27	1.333970342130206E-4	3.253586200317576E-4	0.0	3.1234427523048726E-4	7.808606880762183E-5
28-29	1.7894724101746667E-4	3.253586200317576E-4	0.0	4.587556542447782E-4	8.13396550079394E-5
30-31	1.887079996184194E-4	3.774159992368388E-4	0.0	9.760758600952729E-4	8.784682740857456E-5
32-33	1.887079996184194E-4	3.936839302384267E-4	0.0	0.0020172234441968974	1.0411475841016243E-4
34-35	1.887079996184194E-4	4.4248772324319036E-4	0.0	0.003292629234721387	1.0411475841016243E-4
36-37	1.887079996184194E-4	4.8803793004763643E-4	0.0	0.005173202058504946	1.0411475841016243E-4
38-39	1.887079996184194E-4	5.335881368520825E-4	0.0	0.008475592051827286	1.3014344801270304E-4
40-41	1.9521517201905457E-4	6.019134470587515E-4	0.0	0.012253005630395991	1.3014344801270304E-4
42-43	1.9521517201905457E-4	6.279421366612921E-4	0.0	0.015421998589505312	1.3339703421302062E-4
44-45	1.9521517201905457E-4	7.027746192685964E-4	0.0	0.018971661134051784	1.366506204133382E-4
46-47	1.9521517201905457E-4	7.483248260730425E-4	0.0	0.022866203815831924	1.3990420661365576E-4
48-49	1.9521517201905457E-4	7.515784122733601E-4	0.0	0.026484191670585067	1.496649652146085E-4
50-51	1.9521517201905457E-4	8.622003430841577E-4	0.0	0.030219308628549647	1.496649652146085E-4
52-53	1.9521517201905457E-4	8.654539292844752E-4	0.0	0.03416916227573519	1.6593289621619637E-4
54-55	1.9521517201905457E-4	8.719611016851103E-4	0.0	0.03769604971687944	1.8220082721778425E-4
56-57	1.9521517201905457E-4	8.784682740857455E-4	0.0	0.04130753039923195	1.9196158581873699E-4
58-59	1.9521517201905457E-4	8.817218602860631E-4	0.0	0.044997097150392074	2.0172234441968973E-4
60-61	1.9521517201905457E-4	8.882290326866983E-4	0.0	0.04888187907357126	2.0822951682032486E-4
62-63	1.9521517201905457E-4	8.97989791287651E-4	0.0	0.05252914920412727	2.2124386162159518E-4
64-65	1.9521517201905457E-4	0.0010086117220984485	0.0	0.05575996030104262	2.2124386162159518E-4
66-67	1.9521517201905457E-4	0.0010248796531000365	0.0	0.05916971863897544	2.2449744782191273E-4
68-69	1.9521517201905457E-4	0.0010574155151032122	0.0	0.06276167780412605	2.3425820642286547E-4
70-71	1.9521517201905457E-4	0.0010801906185054352	0.0	0.06595995303903822	2.4727255122413577E-4
72-73	1.9521517201905457E-4	0.0010866977909060704	0.0	0.06869947261970562	2.863155856279467E-4
74-75	1.9521517201905457E-4	0.0011159800667089287	0.0	0.07057353827108853	3.0258351662953455E-4
76-77	1.9521517201905457E-4	0.0011647838597136921	0.0	0.07251918281887845	3.0909068903016976E-4
78-79	1.9521517201905457E-4	0.0011843053769155978	0.0	0.07365468440278929	3.123442752304873E-4
80-81	1.9521517201905457E-4	0.0011843053769155978	0.0	0.07407114343642993	3.286122062320752E-4
82-83	1.9521517201905457E-4	0.0011843053769155978	0.0	0.07432817674625503	3.578944820349334E-4
84-85	1.9521517201905457E-4	0.0011875589631159152	0.0	0.07444855943566678	3.578944820349334E-4
86-87	1.9521517201905457E-4	0.001203826894117503	0.0	0.07450061681487186	3.578944820349334E-4
88	1.9521517201905457E-4	0.001281912962925125	0.0	0.0745071239872725	3.644016544355685E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	23465	0.0	51.76398	1
GTATCAA	44970	0.0	39.142	1
TCAACGC	55750	0.0	31.018576	4
ATCAACG	55955	0.0	30.898386	3
CAACGCA	56595	0.0	30.525074	5
TATCAAC	57735	0.0	30.187506	2
AACGCAG	58450	0.0	29.57402	6
ACGCAGA	66755	0.0	25.805178	7
CGCAGAG	67445	0.0	25.54477	8
GCAGAGT	77720	0.0	21.89635	9
GTACATG	55735	0.0	20.632174	1
TACATGG	55555	0.0	20.27271	2
GAGTACT	44685	0.0	19.796864	12-13
ACATGGG	56655	0.0	19.49839	3
AGAGTAC	71360	0.0	19.262623	10-11
CAGAGTA	76050	0.0	18.870317	10-11
AGTACTT	48465	0.0	17.639462	12-13
GTACTTT	50315	0.0	17.565477	14-15
CATGGGG	35555	0.0	17.25903	4
GTGGTAT	9730	0.0	16.082468	1
>>END_MODULE